Restriction map for
pFN21AB7356
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Aor51HI AscI Asp718I
AspEI AvaI AviII AvrII BamHI
BanI BbeI BcgI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
Cfr10I CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV FseI
FspI HaeII HpaI KasI KpnI
KspI MamI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 1223 TCCGGA T^CCGGA
Alw44I 3142 GTGCAC G^TGCAC
ApaLI 3142 GTGCAC G^TGCAC
AseI 2584 ATTAAT AT^TAAT
AsnI 2584 ATTAAT AT^TAAT
Asp700I 117 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2209 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1662 GCTGAGG CCTCAGC(-5/-2)
BciVI 2641 GGATAC GTATCC(6/5)
BcnI 1309 CCGGG CC^[GC]GG
BsaWI 1223 TCCGGA [AT]^CCGG[AT]
BseAI 1223 TCCGGA T^CCGGA
BsmAI 422 GAGAC GTCTC(1/5)
BsmI 2673 GAATGC GAATGC(1/-1)
BspDI 2905 ATCGAT AT^CGAT
BspEI 1223 TCCGGA T^CCGGA
BsrBI 2432 GAGCGG CCGCTC(-3/-3)
BstAPI 3394 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 835 GGTGACC G^GT[ACGT]ACC
BstPI 835 GGTGACC G^GT[ACGT]ACC
ClaI 2905 ATCGAT AT^CGAT
DraIII 776 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 835 GGTGACC G^GT[ACGT]ACC
EcoT22I 2113 ATGCAT ATGCA^T
HincII 1419 GTTGAC GT[CT]^[GA]AC
HindII 1419 GTTGAC GT[CT]^[GA]AC
HindIII 1615 AAGCTT A^AGCTT
MfeI 1580 CAATTG C^AATTG
MroI 1223 TCCGGA T^CCGGA
MunI 1580 CAATTG C^AATTG
NciI 1309 CCGGG CC^[GC]GG
NsiI 2113 ATGCAT ATGCA^T
PflFI 2209 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2444 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2584 ATTAAT AT^TAAT
PvuII 2941 CAGCTG CAG^CTG
SacI 3468 GAGCTC GAGCT^C
SapI 1288 GCTCTTC GCTCTTC(1/4)
SmlI 3341 CTCAAG C^T[CT][GA]AG
Tth111I 2209 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 117 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 1352 CGCG CG^CG
3265 CGCG
AflIII 136 ACGTGT A^C[GA][CT]GT
2242 ACGTGT
ApaI 1311 GGGCCC GGGCC^C
2876 GGGCCC
BbrPI 135 CACGTG CAC^GTG
2241 CACGTG
BbsI 2866 GAAGAC GAAGAC(2/6)
246 GTCTTC
BclI 1904 TGATCA T^GATCA
2310 TGATCA
BfaI 1966 CTAG C^TAG
3053 CTAG
BpuAI 2866 GAAGAC GAAGAC(2/6)
246 GTCTTC
BsaAI 135 CACGTG [CT]AC^GT[GA]
2241 CACGTG
BseRI 202 GAGGAG GAGGAG(10/8)
3364 GAGGAG
BspHI 81 TCATGA T^CATGA
1883 TCATGA
BsrDI 934 CATTGC GCAATG(2/0)
1937 GCAATG
BstUI 1352 CGCG CG^CG
3265 CGCG
BstXI 3212 CCATGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3480 CCAGAAGCCTGG
CfoI 1351 GCGC GCG^C
2418 GCGC
DrdI 2206 GACGACTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2437 GACGCCAAGGTC
EcoNI 1278 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1716 CCTGGATGAGG
FbaI 1904 TGATCA T^GATCA
2310 TGATCA
HhaI 1351 GCGC GCG^C
2418 GCGC
HinP1I 1351 GCGC G^CGC
2418 GCGC
MaeI 1966 CTAG C^TAG
3053 CTAG
MspA1I 1264 CCGCTG C[AC]G^C[GT]G
2941 CAGCTG
MvnI 1352 CGCG CG^CG
3265 CGCG
NcoI 1913 CCATGG C^CATGG
3212 CCATGG
NspI 1544 GCATGT [GA]CATG^[CT]
2303 GCATGT
PleI 1255 GACTC GAGTC(4/5)
3457 GAGTC
PmaCI 135 CACGTG CAC^GTG
2241 CACGTG
PmlI 135 CACGTG CAC^GTG
2241 CACGTG
RcaI 81 TCATGA T^CATGA
1883 TCATGA
ScaI 427 AGTACT AGT^ACT
473 AGTACT
SspI 1789 AATATT AAT^ATT
2097 AATATT
StuI 1555 AGGCCT AGG^CCT
2512 AGGCCT
XcmI 34 CCAGGTTGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3480 CCAGAAGCCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BalI BanII BbvI BmyI
BpmI BsaHI BsaJI BsgI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PflMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII