(Circular) (Six-base) MAPSORT of: pFN21AB7356.seq Check: 2984 from: 1 to: 8236 pFN21AB7356 8236 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4058 4058 Size: 8236 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6046 278 Size: 53 83 186 936 4510 2468 Fragments arranged by size: 4510 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5579 1937 Size: 3642 4594 Acc65I G'GTAC_C Cuts at: 5557 5557 Size: 8236 AclI AA'CG_TT Cuts at: 1563 6363 6736 1563 Size: 4800 373 3063 Fragments arranged by size: 4800 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 3218 3305 4246 4556 6299 7683 7951 Size: 413 1173 87 941 310 1743 1384 268 Cuts at: 7951 1632 Size: 1917 Fragments arranged by size: 1917 1743 1384 1173 941 413 310 268 87 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8039 AflIII A'CryG_T Cuts at: 1203 2141 4247 7135 8112 1203 Size: 938 2106 2888 977 1327 Fragments arranged by size: 2888 2106 1327 977 938 AgeI A'CCGG_T Cuts at: 7067 7067 Size: 8236 AhdI GACnn_n'nnGTC Cuts at: 6965 6965 Size: 8236 AleI CACnn'nnGTG Cuts at: 1166 4250 1166 Size: 3084 5152 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3419 1184 Size: 2235 6001 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2745 2777 2745 Size: 32 8204 AlwNI CAG_nnn'CTG Cuts at: 2017 2269 3196 3691 3708 4951 5979 7551 2017 Size: 252 927 495 17 1243 1028 1572 2702 Fragments arranged by size: 2702 1572 1243 1028 927 495 252 17 ApaI G_GGCC'C Cuts at: 3320 4885 3320 Size: 1565 6671 ApaLI G'TGCA_C Cuts at: 5147 6293 7449 5147 Size: 1146 1156 5934 Fragments arranged by size: 5934 1156 1146 ApoI r'AATT_y Cuts at: 1493 2375 2791 5208 5545 5771 5923 8032 1493 Size: 882 416 2417 337 226 152 2109 1697 Fragments arranged by size: 2417 2109 1697 882 416 337 226 152 AseI AT'TA_AT Cuts at: 160 1032 4590 6790 160 Size: 872 3558 2200 1606 Fragments arranged by size: 3558 2200 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8236 AvaI C'yCGr_G Cuts at: 1870 1943 5561 1870 Size: 73 3618 4545 Fragments arranged by size: 4545 3618 73 BamHI G'GATC_C Cuts at: 5566 5933 5566 Size: 367 7869 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5557 7012 618 Size: 325 217 627 3770 1455 1842 Fragments arranged by size: 3770 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3320 3594 4885 4932 5426 5477 5555 1391 Size: 1929 274 1291 47 494 51 78 4072 Fragments arranged by size: 4072 1929 1291 494 274 78 51 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2244 4878 928 Size: 971 345 2634 4286 Fragments arranged by size: 4286 2634 971 345 BbvCI CC'TCA_GC Cuts at: 3668 3668 Size: 8236 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6427 6461 1903 Size: 34 50 34 4406 34 3678 Fragments arranged by size: 4406 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4640 6128 7338 4640 Size: 1488 1210 5538 Fragments arranged by size: 5538 1488 1210 BclI T'GATC_A Cuts at: 1548 3909 4315 1548 Size: 2361 406 5469 Fragments arranged by size: 5469 2361 406 BfrBI ATG'CAT Cuts at: 4120 4120 Size: 8236 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6847 136 Size: 107 122 71 631 605 5175 1525 Fragments arranged by size: 5175 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8231 8231 Size: 8236 BlpI GC'TnA_GC Cuts at: 5653 5653 Size: 8236 Bme1580I G_kGCm'C Cuts at: 1790 3320 3500 4885 5151 6297 7453 1790 Size: 1530 180 1385 266 1146 1156 2573 Fragments arranged by size: 2573 1530 1385 1156 1146 266 180 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8236 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5967 6925 442 Size: 416 53 83 399 24 4550 958 1753 Fragments arranged by size: 4550 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8236 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1994 3164 3541 5379 6896 1239 Size: 755 1170 377 1838 1517 2579 Fragments arranged by size: 2579 1838 1517 1170 755 377 Bpu10I CC'TnA_GC Cuts at: 3668 5306 3668 Size: 1638 6598 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5331 6361 7226 7524 5331 Size: 1030 865 298 6043 Fragments arranged by size: 6043 1030 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6899 882 Size: 617 5400 2219 Fragments arranged by size: 5400 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2142 4248 6027 493 Size: 711 938 2106 1779 2702 Fragments arranged by size: 2702 2106 1779 938 711 BsaBI GATnn'nnATC Cuts at: 8230 8230 Size: 8236 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4297 4416 4443 4875 Size: 53 83 186 936 2764 119 27 432 Cuts at: 4875 5390 6043 6425 7060 275 Size: 515 653 382 635 1451 Fragments arranged by size: 2764 1451 936 653 635 515 432 382 186 119 83 53 27 BsaWI w'CCGG_w Cuts at: 3228 6669 7067 7341 7488 7899 3228 Size: 3441 398 274 147 411 3565 Fragments arranged by size: 3565 3441 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2201 2231 1178 Size: 30 165 30 798 30 7183 Fragments arranged by size: 7183 798 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 5384 1186 Size: 1036 3162 4038 Fragments arranged by size: 4038 3162 1036 BseYI C'CCAG_C Cuts at: 3320 5427 7439 7935 3320 Size: 2107 2012 496 3621 Fragments arranged by size: 3621 2107 2012 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2245 2374 3804 5466 1163 Size: 573 509 129 1430 1662 3933 Fragments arranged by size: 3933 1662 1430 573 509 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5686 6447 7475 1540 Size: 275 186 3685 761 1028 2301 Fragments arranged by size: 3685 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3228 5151 5477 6297 6382 7453 3228 Size: 1923 326 820 85 1071 4011 Fragments arranged by size: 4011 1923 1071 820 326 85 BsmI GAATG_Cn' Cuts at: 4684 5747 5840 4684 Size: 1063 93 7080 Fragments arranged by size: 7080 1063 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3228 3320 3500 3594 4885 4932 5151 Size: 399 1438 92 180 94 1291 47 219 Cuts at: 5151 5426 5477 5555 6297 6382 7453 1391 Size: 275 51 78 742 85 1071 2174 Fragments arranged by size: 2174 1438 1291 1071 742 399 275 219 180 94 92 85 78 51 47 BspEI T'CCGG_A Cuts at: 3228 7899 3228 Size: 4671 3565 BspHI T'CATG_A Cuts at: 2086 3888 6125 7855 2086 Size: 1802 2237 1730 2467 Fragments arranged by size: 2467 2237 1802 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2455 4058 5029 5591 844 Size: 1611 1603 971 562 3489 Fragments arranged by size: 3489 1611 1603 971 562 BsrBI CCG'CTC Cuts at: 1394 1823 4439 6123 1394 Size: 429 2616 1684 3507 Fragments arranged by size: 3507 2616 1684 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2938 3949 6731 6905 65 Size: 1183 1690 1011 2782 174 1396 Fragments arranged by size: 2782 1690 1396 1183 1011 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6880 7067 1700 Size: 110 5070 187 2869 Fragments arranged by size: 5070 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8236 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8236 BssSI C'ACGA_G Cuts at: 6296 7308 6296 Size: 1012 7224 BstAPI GCAn_nnn'nTGC Cuts at: 794 5405 794 Size: 4611 3625 BstBI TT'CG_AA Cuts at: 7040 7040 Size: 8236 BstEII G'GTnAC_C Cuts at: 1184 2840 1184 Size: 1656 6580 BstXI CCAn_nnnn'nTGG Cuts at: 5224 5492 5224 Size: 268 7968 BstYI r'GATC_y Cuts at: 1972 2035 4566 4720 5566 5933 6318 6335 7787 Size: 63 2531 154 846 367 385 17 1452 Cuts at: 7787 7873 7885 8231 1972 Size: 86 12 346 1977 Fragments arranged by size: 2531 1977 1452 846 385 367 346 154 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 2846 3701 5433 2846 Size: 855 1732 5649 Fragments arranged by size: 5649 1732 855 BtgI C'CryG_G Cuts at: 513 1065 3216 3918 5217 513 Size: 552 2151 702 1299 3532 Fragments arranged by size: 3532 2151 1299 702 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7497 Fragments arranged by size: 7497 683 56 BtsI GCAGTG_nn' Cuts at: 5752 6546 6566 5752 Size: 794 20 7422 Fragments arranged by size: 7422 794 20 ClaI AT'CG_AT Cuts at: 1554 4911 1554 Size: 3357 4879 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8201 DraI TTT'AAA Cuts at: 2952 3141 3787 5172 5540 2952 Size: 189 646 1385 368 5648 Fragments arranged by size: 5648 1385 646 368 189 DraIII CAC_nnn'GTG Cuts at: 2786 2786 Size: 8236 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4217 4448 7243 809 Size: 507 2901 231 2795 1802 Fragments arranged by size: 2901 2795 1802 507 231 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2735 2990 4230 Size: 54 1425 50 275 18 905 255 1240 Cuts at: 4230 4696 5683 6572 8201 8 Size: 466 987 889 1629 43 Fragments arranged by size: 1629 1425 1240 987 905 889 466 275 255 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5683 1537 Size: 275 3871 4090 Fragments arranged by size: 4090 3871 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3300 3570 3918 6166 1353 Size: 1947 270 348 2248 3423 Fragments arranged by size: 3423 2248 1947 348 270 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6823 7197 7343 387 Size: 1064 138 5234 374 146 1280 Fragments arranged by size: 5234 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5475 5475 Size: 8236 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1994 2045 3164 3218 3305 3541 4246 Size: 393 362 51 1119 54 87 236 705 Cuts at: 4246 4556 5379 6299 6896 7683 7951 1239 Size: 310 823 920 597 787 268 1524 Fragments arranged by size: 1524 1119 920 823 787 705 597 393 362 310 268 236 87 54 51 EcoNI CCTnn'n_nnAGG Cuts at: 3287 3725 3287 Size: 438 7798 EcoO109I rG'GnC_Cy Cuts at: 3202 3317 4450 4882 3202 Size: 115 1133 432 6556 Fragments arranged by size: 6556 1133 432 115 EcoRI G'AATT_C Cuts at: 5545 5923 5545 Size: 378 7858 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3265 3417 3265 Size: 152 8084 FspI TGC'GCA Cuts at: 6742 6742 Size: 8236 HaeII r_GCGC'y Cuts at: 1678 7383 1678 Size: 5705 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2020 2052 3155 3187 5348 Size: 32 135 32 448 32 1103 32 2161 Cuts at: 5348 5380 6877 6909 6951 6983 1373 Size: 32 1497 32 42 32 2626 Fragments arranged by size: 2626 2161 1497 1103 448 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3426 5580 5826 669 Size: 988 281 1488 2154 246 3079 Fragments arranged by size: 3079 2154 1488 988 281 246 HindIII A'AGCT_T Cuts at: 748 3620 748 Size: 2872 5364 HpaI GTT'AAC Cuts at: 5826 5826 Size: 8236 KpnI G_GTAC'C Cuts at: 5561 5561 Size: 8236 MfeI C'AATT_G Cuts at: 3585 5835 3585 Size: 2250 5986 MluI A'CGCG_T Cuts at: 8112 8112 Size: 8236 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3823 3973 4833 5062 7350 7534 1305 Size: 2518 150 860 229 2288 184 2007 Fragments arranged by size: 2518 2288 2007 860 229 184 150 MscI TGG'CCA Cuts at: 10 64 1489 1832 2737 2992 4232 10 Size: 54 1425 343 905 255 1240 4014 Fragments arranged by size: 4014 1425 1240 905 343 255 54 MslI CAynn'nnrTG Cuts at: 518 1166 3917 4250 5222 6194 6553 6712 518 Size: 648 2751 333 972 972 359 159 2042 Fragments arranged by size: 2751 2042 972 972 648 359 333 159 MspA1I CmG'CkG Cuts at: 1606 3271 4948 5652 6329 7477 7722 1606 Size: 1665 1677 704 677 1148 245 2120 Fragments arranged by size: 2120 1677 1665 1148 704 677 245 NcoI C'CATG_G Cuts at: 513 1065 3918 5217 513 Size: 552 2853 1299 3532 Fragments arranged by size: 3532 2853 1299 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8236 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8236 NotI GC'GGCC_GC Cuts at: 5683 5683 Size: 8236 NsiI A_TGCA'T Cuts at: 4122 4122 Size: 8236 NspI r_CATG'y Cuts at: 1132 1229 3553 4312 5594 7139 1132 Size: 97 2324 759 1282 1545 2229 Fragments arranged by size: 2324 2229 1545 1282 759 97 PasI CC'CwG_GG Cuts at: 1348 3500 1348 Size: 2152 6084 PciI A'CATG_T Cuts at: 7135 7135 Size: 8236 PflMI CCAn_nnn'nTGG Cuts at: 1108 2017 2269 2457 4411 1108 Size: 909 252 188 1954 4933 Fragments arranged by size: 4933 1954 909 252 188 PfoI T'CCnGG_A Cuts at: 3720 3720 Size: 8236 PmeI GTTT'AAAC Cuts at: 5540 5540 Size: 8236 PmlI CAC'GTG Cuts at: 2142 4248 2142 Size: 2106 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6305 6337 7818 7850 6305 Size: 32 1481 32 6691 Fragments arranged by size: 6691 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4450 4450 Size: 8236 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8236 PsiI TTA'TAA Cuts at: 2060 5193 5806 2060 Size: 3133 613 4490 Fragments arranged by size: 4490 3133 613 PspOMI G'GGCC_C Cuts at: 3316 4881 3316 Size: 1565 6671 PstI C_TGCA'G Cuts at: 830 3135 3210 4348 5421 5588 830 Size: 2305 75 1138 1073 167 3478 Fragments arranged by size: 3478 2305 1138 1073 167 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8236 PvuII CAG'CTG Cuts at: 4948 4948 Size: 8236 SacI G_AGCT'C Cuts at: 5477 5477 Size: 8236 SalI G'TCGA_C Cuts at: 1936 5578 1936 Size: 3642 4594 SapI GCTCTTCn'nnn_ Cuts at: 3300 3300 Size: 8236 SbfI CC_TGCA'GG Cuts at: 5588 5588 Size: 8236 ScaI AGT'ACT Cuts at: 2434 2480 6484 8196 2434 Size: 46 4004 1712 2474 Fragments arranged by size: 4004 2474 1712 46 SfcI C'TryA_G Cuts at: 826 1045 2362 3131 3206 4056 4344 5008 5417 Size: 219 1317 769 75 850 288 664 409 Cuts at: 5417 5584 6719 7400 7591 826 Size: 167 1135 681 191 1471 Fragments arranged by size: 1471 1317 1135 850 769 681 664 409 288 219 191 167 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8236 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8236 SmaI CCC'GGG Cuts at: 1872 5563 1872 Size: 3691 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5346 6340 7241 7503 820 Size: 197 926 3403 994 901 262 1553 Fragments arranged by size: 3403 1553 994 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6027 493 Size: 5534 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8236 SphI G_CATG'C Cuts at: 1132 5594 1132 Size: 4462 3774 SspI AAT'ATT Cuts at: 5 52 3796 4104 6160 5 Size: 47 3744 308 2056 2081 Fragments arranged by size: 3744 2081 2056 308 47 StuI AGG'CCT Cuts at: 1517 3562 4519 1517 Size: 2045 957 5234 Fragments arranged by size: 5234 2045 957 StyI C'CwwG_G Cuts at: 513 1065 3626 3918 4446 5217 5675 513 Size: 552 2561 292 528 771 458 3074 Fragments arranged by size: 3074 2561 771 552 528 458 292 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3492 4593 6263 6280 6433 6618 7063 Size: 1255 1912 1101 1670 17 153 185 445 Cuts at: 7063 325 Size: 1498 Fragments arranged by size: 1912 1670 1498 1255 1101 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2385 2432 2478 Size: 274 80 33 51 1443 408 47 46 Cuts at: 2478 2985 3958 6482 8194 96 Size: 507 973 2524 1712 138 Fragments arranged by size: 2524 1712 1443 973 507 408 274 138 80 51 47 46 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2201 2233 8196 8228 2201 Size: 32 5963 32 2209 Fragments arranged by size: 5963 2209 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4217 1316 Size: 2901 5335 XbaI T'CTAG_A Cuts at: 5572 5572 Size: 8236 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2046 5492 2046 Size: 3446 4790 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8236 XmaI C'CCGG_G Cuts at: 1870 5561 1870 Size: 3691 4545 XmnI GAAnn'nnTTC Cuts at: 2126 6365 2126 Size: 4239 3997 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6044 276 Size: 53 83 186 936 4510 2468 Fragments arranged by size: 4510 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI