Restriction map for
pFN21AB7356
(Linear) (Six-base) MAPSORT of: KIBB7356.seq Check: 801 from: 1 to: 3531
KIBB7356.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2053 3531
Size: 2053 1478
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1213 1300 2241 2551 3531
Size: 40 1173 87 941 310 980
Fragments arranged by size:
1173 980 941 310 87 40
AflIII A'CryG_T
Cuts at: 0 136 2242 3531
Size: 136 2106 1289
Fragments arranged by size:
2106 1289 136
AleI CACnn'nnGTG
Cuts at: 0 2245 3531
Size: 2245 1286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1414 3531
Size: 1414 2117
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 740 772 3531
Size: 740 32 2759
Fragments arranged by size:
2759 740 32
AlwNI CAG_nnn'CTG
Cuts at: 0 12 264 1191 1686 1703 2946 3531
Size: 12 252 927 495 17 1243 585
Fragments arranged by size:
1243 927 585 495 252 17 12
ApaI G_GGCC'C
Cuts at: 0 1315 2880 3531
Size: 1315 1565 651
Fragments arranged by size:
1565 1315 651
ApaLI G'TGCA_C
Cuts at: 0 3142 3531
Size: 3142 389
ApoI r'AATT_y
Cuts at: 0 370 786 3203 3531
Size: 370 416 2417 328
Fragments arranged by size:
2417 416 370 328
AseI AT'TA_AT
Cuts at: 0 2585 3531
Size: 2585 946
BanII G_rGCy'C
Cuts at: 0 1315 1589 2880 2927 3421 3472 3531
Size: 1315 274 1291 47 494 51 59
Fragments arranged by size:
1315 1291 494 274 59 51 47
BbsI GAAGACnn'nnnn_
Cuts at: 0 239 2873 3531
Size: 239 2634 658
Fragments arranged by size:
2634 658 239
BbvCI CC'TCA_GC
Cuts at: 0 1663 3531
Size: 1663 1868
BciVI GTATCCnnnnn_n'
Cuts at: 0 2635 3531
Size: 2635 896
BclI T'GATC_A
Cuts at: 0 1904 2310 3531
Size: 1904 406 1221
Fragments arranged by size:
1904 1221 406
BfrBI ATG'CAT
Cuts at: 0 2115 3531
Size: 2115 1416
Bme1580I G_kGCm'C
Cuts at: 0 1315 1495 2880 3146 3531
Size: 1315 180 1385 266 385
Fragments arranged by size:
1385 1315 385 266 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1536 3374 3531
Size: 1159 377 1838 157
Fragments arranged by size:
1838 1159 377 157
Bpu10I CC'TnA_GC
Cuts at: 0 1663 3301 3531
Size: 1663 1638 230
Fragments arranged by size:
1663 1638 230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3326 3531
Size: 3326 205
BsaAI yAC'GTr
Cuts at: 0 137 2243 3531
Size: 137 2106 1288
Fragments arranged by size:
2106 1288 137
BsaHI Gr'CG_yC
Cuts at: 0 2292 2411 2438 2870 3385 3531
Size: 2292 119 27 432 515 146
Fragments arranged by size:
2292 515 432 146 119 27
BsaWI w'CCGG_w
Cuts at: 0 1223 3531
Size: 1223 2308
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 196 226 3531
Size: 196 30 3305
Fragments arranged by size:
3305 196 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 3379 3531
Size: 217 3162 152
Fragments arranged by size:
3162 217 152
BseYI C'CCAG_C
Cuts at: 0 1315 3422 3531
Size: 1315 2107 109
Fragments arranged by size:
2107 1315 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 369 1799 3461 3531
Size: 240 129 1430 1662 70
Fragments arranged by size:
1662 1430 240 129 70
BsiHKAI G_wGCw'C
Cuts at: 0 1223 3146 3472 3531
Size: 1223 1923 326 59
Fragments arranged by size:
1923 1223 326 59
BsmI GAATG_Cn'
Cuts at: 0 2679 3531
Size: 2679 852
Bsp1286I G_dGCh'C
Cuts at: 0 1223 1315 1495 1589 2880 2927 3146 3421
Size: 1223 92 180 94 1291 47 219 275
Cuts at: 3421 3472 3531
Size: 51 59
Fragments arranged by size:
1291 1223 275 219 180 94 92 59
51 47
BspEI T'CCGG_A
Cuts at: 0 1223 3531
Size: 1223 2308
BspHI T'CATG_A
Cuts at: 0 81 1883 3531
Size: 81 1802 1648
Fragments arranged by size:
1802 1648 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 450 2053 3024 3531
Size: 450 1603 971 507
Fragments arranged by size:
1603 971 507 450
BsrBI CCG'CTC
Cuts at: 0 2434 3531
Size: 2434 1097
BsrDI GCAATG_nn'
Cuts at: 0 933 1944 3531
Size: 933 1011 1587
Fragments arranged by size:
1587 1011 933
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3400 3531
Size: 3400 131
BstEII G'GTnAC_C
Cuts at: 0 835 3531
Size: 835 2696
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3219 3487 3531
Size: 3219 268 44
Fragments arranged by size:
3219 268 44
BstYI r'GATC_y
Cuts at: 0 30 2561 2715 3531
Size: 30 2531 154 816
Fragments arranged by size:
2531 816 154 30
Bsu36I CC'TnA_GG
Cuts at: 0 841 1696 3428 3531
Size: 841 855 1732 103
Fragments arranged by size:
1732 855 841 103
BtgI C'CryG_G
Cuts at: 0 1211 1913 3212 3531
Size: 1211 702 1299 319
Fragments arranged by size:
1299 1211 702 319
ClaI AT'CG_AT
Cuts at: 0 2906 3531
Size: 2906 625
DraI TTT'AAA
Cuts at: 0 947 1136 1782 3167 3531
Size: 947 189 646 1385 364
Fragments arranged by size:
1385 947 646 364 189
DraIII CAC_nnn'GTG
Cuts at: 0 781 3531
Size: 781 2750
DrdI GACnn_nn'nnGTC
Cuts at: 0 2212 2443 3531
Size: 2212 231 1088
Fragments arranged by size:
2212 1088 231
EaeI y'GGCC_r
Cuts at: 0 730 985 2225 2691 3531
Size: 730 255 1240 466 840
Fragments arranged by size:
1240 840 730 466 255
EarI CTCTTCn'nnn_
Cuts at: 0 1295 1565 1913 3531
Size: 1295 270 348 1618
Fragments arranged by size:
1618 1295 348 270
EcoICRI GAG'CTC
Cuts at: 0 3470 3531
Size: 3470 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1159 1213 1300 1536 2241 2551 3374
Size: 40 1119 54 87 236 705 310 823
Cuts at: 3374 3531
Size: 157
Fragments arranged by size:
1119 823 705 310 236 157 87 54
40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1282 1720 3531
Size: 1282 438 1811
Fragments arranged by size:
1811 1282 438
EcoO109I rG'GnC_Cy
Cuts at: 0 1197 1312 2445 2877 3531
Size: 1197 115 1133 432 654
Fragments arranged by size:
1197 1133 654 432 115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1260 1412 3531
Size: 1260 152 2119
Fragments arranged by size:
2119 1260 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 15 47 1150 1182 3343 3375 3531
Size: 15 32 1103 32 2161 32 156
Fragments arranged by size:
2161 1103 156 32 32 32 15
HincII GTy'rAC
Cuts at: 0 1421 3531
Size: 1421 2110
HindIII A'AGCT_T
Cuts at: 0 1615 3531
Size: 1615 1916
MfeI C'AATT_G
Cuts at: 0 1580 3531
Size: 1580 1951
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1818 1968 2828 3057 3531
Size: 1818 150 860 229 474
Fragments arranged by size:
1818 860 474 229 150
MscI TGG'CCA
Cuts at: 0 732 987 2227 3531
Size: 732 255 1240 1304
Fragments arranged by size:
1304 1240 732 255
MslI CAynn'nnrTG
Cuts at: 0 1912 2245 3217 3531
Size: 1912 333 972 314
Fragments arranged by size:
1912 972 333 314
MspA1I CmG'CkG
Cuts at: 0 1266 2943 3531
Size: 1266 1677 588
Fragments arranged by size:
1677 1266 588
NcoI C'CATG_G
Cuts at: 0 1913 3212 3531
Size: 1913 1299 319
Fragments arranged by size:
1913 1299 319
NsiI A_TGCA'T
Cuts at: 0 2117 3531
Size: 2117 1414
NspI r_CATG'y
Cuts at: 0 1548 2307 3531
Size: 1548 759 1224
Fragments arranged by size:
1548 1224 759
PasI CC'CwG_GG
Cuts at: 0 1495 3531
Size: 1495 2036
PflMI CCAn_nnn'nTGG
Cuts at: 0 12 264 452 2406 3531
Size: 12 252 188 1954 1125
Fragments arranged by size:
1954 1125 252 188 12
PfoI T'CCnGG_A
Cuts at: 0 1715 3531
Size: 1715 1816
PmlI CAC'GTG
Cuts at: 0 137 2243 3531
Size: 137 2106 1288
Fragments arranged by size:
2106 1288 137
PpuMI rG'GwC_Cy
Cuts at: 0 2445 3531
Size: 2445 1086
PsiI TTA'TAA
Cuts at: 0 55 3188 3531
Size: 55 3133 343
Fragments arranged by size:
3133 343 55
PspOMI G'GGCC_C
Cuts at: 0 1311 2876 3531
Size: 1311 1565 655
Fragments arranged by size:
1565 1311 655
PstI C_TGCA'G
Cuts at: 0 1130 1205 2343 3416 3531
Size: 1130 75 1138 1073 115
Fragments arranged by size:
1138 1130 1073 115 75
PvuII CAG'CTG
Cuts at: 0 2943 3531
Size: 2943 588
SacI G_AGCT'C
Cuts at: 0 3472 3531
Size: 3472 59
SapI GCTCTTCn'nnn_
Cuts at: 0 1295 3531
Size: 1295 2236
ScaI AGT'ACT
Cuts at: 0 429 475 3531
Size: 429 46 3056
Fragments arranged by size:
3056 429 46
SfcI C'TryA_G
Cuts at: 0 357 1126 1201 2051 2339 3003 3412 3531
Size: 357 769 75 850 288 664 409 119
Fragments arranged by size:
850 769 664 409 357 288 119 75
SmlI C'TyrA_G
Cuts at: 0 3341 3531
Size: 3341 190
SspI AAT'ATT
Cuts at: 0 1791 2099 3531
Size: 1791 308 1432
Fragments arranged by size:
1791 1432 308
StuI AGG'CCT
Cuts at: 0 1557 2514 3531
Size: 1557 957 1017
Fragments arranged by size:
1557 1017 957
StyI C'CwwG_G
Cuts at: 0 1621 1913 2441 3212 3531
Size: 1621 292 528 771 319
Fragments arranged by size:
1621 771 528 319 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1487 2588 3531
Size: 1487 1101 943
Fragments arranged by size:
1487 1101 943
TatI w'GTAC_w
Cuts at: 0 380 427 473 980 1953 3531
Size: 380 47 46 507 973 1578
Fragments arranged by size:
1578 973 507 380 47 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 196 228 3531
Size: 196 32 3303
Fragments arranged by size:
3303 196 32
Tth111I GACn'n_nGTC
Cuts at: 0 2212 3531
Size: 2212 1319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 41 3487 3531
Size: 41 3446 44
Fragments arranged by size:
3446 44 41
XmnI GAAnn'nnTTC
Cuts at: 0 121 3531
Size: 121 3410
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI BanII BbsI BbvCI BciVI BclI
BfrBI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BstAPI BstEII BstXI BstYI
Bsu36I BtgI ClaI DraI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII
MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI
PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PstI
PvuII SacI SapI ScaI SfcI SmlI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BcgI BglI BglII BlpI BmgBI BmrI BmtI BplI
BsaI BsaBI BsiEI BsiWI BsmBI BsrFI BsrGI BssHII
BssSI BstBI BstZ17I BtgZI BtsI CspCI EagI EciI
EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI
KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PciI PmeI PpiI PshAI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI XbaI XhoI XmaI ZraI