(Circular) (Six-base) MAPSORT of: pFN21AB7354.seq Check: 3679 from: 1 to: 8236 pFN21AB7354 8236 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6046 278 Size: 53 83 186 936 4510 2468 Fragments arranged by size: 4510 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2784 2930 3307 4809 5579 1937 Size: 847 146 377 1502 770 4594 Fragments arranged by size: 4594 1502 847 770 377 146 Acc65I G'GTAC_C Cuts at: 5557 5557 Size: 8236 AclI AA'CG_TT Cuts at: 1563 6363 6736 1563 Size: 4800 373 3063 Fragments arranged by size: 4800 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2573 2922 2964 3324 3672 3747 4894 6299 Size: 941 349 42 360 348 75 1147 1405 Cuts at: 6299 7683 7951 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1405 1384 1147 941 360 349 348 268 75 42 AflII C'TTAA_G Cuts at: 820 1017 4870 820 Size: 197 3853 4186 Fragments arranged by size: 4186 3853 197 AflIII A'CryG_T Cuts at: 1203 2560 3213 5249 7135 8112 1203 Size: 1357 653 2036 1886 977 1327 Fragments arranged by size: 2036 1886 1357 1327 977 653 AgeI A'CCGG_T Cuts at: 7067 7067 Size: 8236 AhdI GACnn_n'nnGTC Cuts at: 6965 6965 Size: 8236 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8236 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8236 AlwNI CAG_nnn'CTG Cuts at: 2029 2306 3486 5203 5979 7551 2029 Size: 277 1180 1717 776 1572 2714 Fragments arranged by size: 2714 1717 1572 1180 776 277 ApaLI G'TGCA_C Cuts at: 6293 7449 6293 Size: 1156 7080 ApoI r'AATT_y Cuts at: 1493 2117 2161 2176 2460 2766 3098 3716 3846 Size: 624 44 15 284 306 332 618 130 Cuts at: 3846 3860 3905 5545 5771 5923 8032 1493 Size: 14 45 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 624 618 332 306 284 226 152 130 45 44 15 14 AseI AT'TA_AT Cuts at: 160 1032 3276 3300 6790 160 Size: 872 2244 24 3490 1606 Fragments arranged by size: 3490 2244 1606 872 24 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8236 AvaI C'yCGr_G Cuts at: 1870 1943 5561 1870 Size: 73 3618 4545 Fragments arranged by size: 4545 3618 73 BamHI G'GATC_C Cuts at: 2016 4937 5473 5566 5933 2016 Size: 2921 536 93 367 4319 Fragments arranged by size: 4319 2921 536 367 93 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3556 3757 5557 7012 618 Size: 325 217 627 1769 201 1800 1455 1842 Fragments arranged by size: 1842 1800 1769 1455 627 325 217 201 BanII G_rGCy'C Cuts at: 1391 2371 5171 5555 1391 Size: 980 2800 384 4072 Fragments arranged by size: 4072 2800 980 384 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2561 4053 4540 5011 928 Size: 971 662 1492 487 471 4153 Fragments arranged by size: 4153 1492 971 662 487 471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6427 6461 1903 Size: 34 4490 34 3678 Fragments arranged by size: 4490 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6128 7338 6128 Size: 1210 7026 BclI T'GATC_A Cuts at: 1548 2676 1548 Size: 1128 7108 BfrBI ATG'CAT Cuts at: 3581 3581 Size: 8236 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6847 136 Size: 107 122 71 631 605 5175 1525 Fragments arranged by size: 5175 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8231 8231 Size: 8236 BlpI GC'TnA_GC Cuts at: 5653 5653 Size: 8236 Bme1580I G_kGCm'C Cuts at: 1790 6297 7453 1790 Size: 4507 1156 2573 Fragments arranged by size: 4507 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8236 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4131 4675 5967 Size: 416 53 83 399 24 2714 544 1292 Cuts at: 5967 6925 442 Size: 958 1753 Fragments arranged by size: 2714 1753 1292 958 544 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8236 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5168 5168 Size: 8236 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6896 1239 Size: 5657 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3150 6361 7226 7524 3150 Size: 3211 865 298 3862 Fragments arranged by size: 3862 3211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6899 882 Size: 617 5400 2219 Fragments arranged by size: 5400 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2074 3214 6027 493 Size: 711 870 1140 2813 2702 Fragments arranged by size: 2813 2702 1140 870 711 BsaBI GATnn'nnATC Cuts at: 3132 3138 3726 8230 3132 Size: 6 588 4504 3138 Fragments arranged by size: 4504 3138 588 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6043 6425 7060 275 Size: 53 83 186 936 4510 382 635 1451 Fragments arranged by size: 4510 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6669 7067 7341 7488 7899 6669 Size: 398 274 147 411 7006 Fragments arranged by size: 7006 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2358 2388 3727 3757 1178 Size: 30 165 30 955 30 1339 30 5657 Fragments arranged by size: 5657 1339 955 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 8236 BseYI C'CCAG_C Cuts at: 2029 3123 7439 7935 2029 Size: 1094 4316 496 2330 Fragments arranged by size: 4316 2330 1094 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3508 1163 Size: 573 1772 5891 Fragments arranged by size: 5891 1772 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5686 6447 7475 1540 Size: 275 186 3685 761 1028 2301 Fragments arranged by size: 3685 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2371 6297 6382 7453 2371 Size: 3926 85 1071 3154 Fragments arranged by size: 3926 3154 1071 85 BsmI GAATG_Cn' Cuts at: 2636 5415 5747 5840 2636 Size: 2779 332 93 5032 Fragments arranged by size: 5032 2779 332 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2371 5171 5555 6297 6382 7453 1391 Size: 399 581 2800 384 742 85 1071 2174 Fragments arranged by size: 2800 2174 1071 742 581 399 384 85 BspEI T'CCGG_A Cuts at: 7899 7899 Size: 8236 BspHI T'CATG_A Cuts at: 3142 6125 7855 3142 Size: 2983 1730 3523 Fragments arranged by size: 3523 2983 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5330 5591 844 Size: 4486 261 3489 Fragments arranged by size: 4486 3489 261 BsrBI CCG'CTC Cuts at: 1394 1823 6123 1394 Size: 429 4300 3507 Fragments arranged by size: 4300 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3514 6731 6905 65 Size: 1183 2266 3217 174 1396 Fragments arranged by size: 3217 2266 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6880 7067 1700 Size: 110 5070 187 2869 Fragments arranged by size: 5070 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3496 4639 96 Size: 3400 1143 3693 Fragments arranged by size: 3693 3400 1143 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8236 BssSI C'ACGA_G Cuts at: 6296 7308 6296 Size: 1012 7224 BstAPI GCAn_nnn'nTGC Cuts at: 794 5393 794 Size: 4599 3637 BstBI TT'CG_AA Cuts at: 2386 2443 7040 2386 Size: 57 4597 3582 Fragments arranged by size: 4597 3582 57 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8236 BstXI CCAn_nnnn'nTGG Cuts at: 4991 4991 Size: 8236 BstYI r'GATC_y Cuts at: 1972 2016 3127 4937 5105 5473 5566 5933 6318 Size: 44 1111 1810 168 368 93 367 385 Cuts at: 6318 6335 7787 7873 7885 8231 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1810 1452 1111 385 368 367 346 168 93 86 44 17 12 BstZ17I GTA'TAC Cuts at: 2785 2931 2785 Size: 146 8090 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7684 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3546 487 Size: 683 56 2320 5177 Fragments arranged by size: 5177 2320 683 56 BtsI GCAGTG_nn' Cuts at: 4394 5752 6546 6566 4394 Size: 1358 794 20 6064 Fragments arranged by size: 6064 1358 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8236 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8201 DraI TTT'AAA Cuts at: 3472 5540 3472 Size: 2068 6168 DraIII CAC_nnn'GTG Cuts at: 2157 2157 Size: 8236 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7243 809 Size: 507 5927 1802 Fragments arranged by size: 5927 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4165 4981 5156 Size: 54 1425 50 275 18 2335 816 175 Cuts at: 5156 5683 6572 8201 8 Size: 527 889 1629 43 Fragments arranged by size: 2335 1629 1425 889 816 527 275 175 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5683 1537 Size: 275 3871 4090 Fragments arranged by size: 4090 3871 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2191 3186 3343 3470 3972 6166 1353 Size: 838 995 157 127 502 2194 3423 Fragments arranged by size: 3423 2194 995 838 502 157 127 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6823 7197 7343 387 Size: 1064 138 5234 374 146 1280 Fragments arranged by size: 5234 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2369 2369 Size: 8236 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2573 2922 2964 3324 3672 3747 4894 Size: 393 941 349 42 360 348 75 1147 Cuts at: 4894 6299 6896 7683 7951 1239 Size: 1405 597 787 268 1524 Fragments arranged by size: 1524 1405 1147 941 787 597 393 360 349 348 268 75 42 EcoRI G'AATT_C Cuts at: 3846 5545 5923 3846 Size: 1699 378 6159 Fragments arranged by size: 6159 1699 378 EcoRV GAT'ATC Cuts at: 5266 5266 Size: 8236 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5296 5296 Size: 8236 FspI TGC'GCA Cuts at: 6742 6742 Size: 8236 HaeII r_GCGC'y Cuts at: 1678 2014 7383 1678 Size: 336 5369 2531 Fragments arranged by size: 5369 2531 336 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2661 2693 2866 2898 4774 Size: 32 135 32 1089 32 173 32 1876 Cuts at: 4774 4806 5074 5106 6877 6909 6951 6983 1373 Size: 32 268 32 1771 32 42 32 2626 Fragments arranged by size: 2626 1876 1771 1089 268 173 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5580 5826 669 Size: 988 281 3642 246 3079 Fragments arranged by size: 3642 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8236 HpaI GTT'AAC Cuts at: 5826 5826 Size: 8236 KpnI G_GTAC'C Cuts at: 5561 5561 Size: 8236 MfeI C'AATT_G Cuts at: 4235 5835 4235 Size: 1600 6636 MluI A'CGCG_T Cuts at: 8112 8112 Size: 8236 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3549 3573 5383 5465 7350 7534 1305 Size: 2244 24 1810 82 1885 184 2007 Fragments arranged by size: 2244 2007 1885 1810 184 82 24 MscI TGG'CCA Cuts at: 10 64 1489 1832 4167 4983 5158 10 Size: 54 1425 343 2335 816 175 3088 Fragments arranged by size: 3088 2335 1425 816 343 175 54 MslI CAynn'nnrTG Cuts at: 518 1166 2052 2426 2792 3855 5027 6194 6553 Size: 648 886 374 366 1063 1172 1167 359 Cuts at: 6553 6712 518 Size: 159 2042 Fragments arranged by size: 2042 1172 1167 1063 886 648 374 366 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 3973 3992 4142 4370 5652 6329 7477 Size: 423 1944 19 150 228 1282 677 1148 Cuts at: 7477 7722 1606 Size: 245 2120 Fragments arranged by size: 2120 1944 1282 1148 677 423 245 228 150 19 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7684 NdeI CA'TA_TG Cuts at: 387 387 Size: 8236 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8236 NotI GC'GGCC_GC Cuts at: 5683 5683 Size: 8236 NsiI A_TGCA'T Cuts at: 3583 3583 Size: 8236 NspI r_CATG'y Cuts at: 1132 1229 2564 4808 5253 5594 7139 1132 Size: 97 1335 2244 445 341 1545 2229 Fragments arranged by size: 2244 2229 1545 1335 445 341 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8236 PciI A'CATG_T Cuts at: 2560 5249 7135 2560 Size: 2689 1886 3661 Fragments arranged by size: 3661 2689 1886 PflMI CCAn_nnn'nTGG Cuts at: 1108 2692 1108 Size: 1584 6652 PfoI T'CCnGG_A Cuts at: 5373 5373 Size: 8236 PmeI GTTT'AAAC Cuts at: 5540 5540 Size: 8236 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3447 3479 6305 6337 7818 7850 3447 Size: 32 2826 32 1481 32 3833 Fragments arranged by size: 3833 2826 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8236 PsiI TTA'TAA Cuts at: 4202 4656 5806 4202 Size: 454 1150 6632 Fragments arranged by size: 6632 1150 454 PstI C_TGCA'G Cuts at: 830 2473 2848 4401 5588 830 Size: 1643 375 1553 1187 3478 Fragments arranged by size: 3478 1643 1553 1187 375 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8236 PvuII CAG'CTG Cuts at: 2029 3992 4142 4370 2029 Size: 1963 150 228 5895 Fragments arranged by size: 5895 1963 228 150 SacI G_AGCT'C Cuts at: 2371 2371 Size: 8236 SalI G'TCGA_C Cuts at: 1936 5578 1936 Size: 3642 4594 SapI GCTCTTCn'nnn_ Cuts at: 3186 3186 Size: 8236 SbfI CC_TGCA'GG Cuts at: 5588 5588 Size: 8236 ScaI AGT'ACT Cuts at: 3575 5308 6484 8196 3575 Size: 1733 1176 1712 3615 Fragments arranged by size: 3615 1733 1712 1176 SfcI C'TryA_G Cuts at: 826 1045 2469 2844 4397 5584 6719 7400 7591 Size: 219 1424 375 1553 1187 1135 681 191 Cuts at: 7591 826 Size: 1471 Fragments arranged by size: 1553 1471 1424 1187 1135 681 375 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8236 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8236 SmaI CCC'GGG Cuts at: 1872 5563 1872 Size: 3691 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3165 4870 6340 7241 7503 820 Size: 197 926 1222 1705 1470 901 262 1553 Fragments arranged by size: 1705 1553 1470 1222 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2074 6027 493 Size: 1581 3953 2702 Fragments arranged by size: 3953 2702 1581 SpeI A'CTAG_T Cuts at: 152 152 Size: 8236 SphI G_CATG'C Cuts at: 1132 5594 1132 Size: 4462 3774 SspI AAT'ATT Cuts at: 5 52 4756 6160 5 Size: 47 4704 1404 2081 Fragments arranged by size: 4704 2081 1404 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8236 StyI C'CwwG_G Cuts at: 513 1065 2963 5675 513 Size: 552 1898 2712 3074 Fragments arranged by size: 3074 2712 1898 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2420 5301 6263 6280 6433 6618 7063 Size: 1255 840 2881 962 17 153 185 445 Cuts at: 7063 325 Size: 1498 Fragments arranged by size: 2881 1498 1255 962 840 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 3496 3573 Size: 274 80 33 51 1443 171 1348 77 Cuts at: 3573 4639 5306 6482 8194 96 Size: 1066 667 1176 1712 138 Fragments arranged by size: 1712 1443 1348 1176 1066 667 274 171 138 80 77 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3727 3759 5348 5380 8196 8228 3727 Size: 32 1589 32 2816 32 3735 Fragments arranged by size: 3735 2816 1589 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8236 XbaI T'CTAG_A Cuts at: 5572 5572 Size: 8236 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3299 5236 3299 Size: 1937 6299 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8236 XmaI C'CCGG_G Cuts at: 1870 5561 1870 Size: 3691 4545 XmnI GAAnn'nnTTC Cuts at: 2463 4363 6365 2463 Size: 1900 2002 4334 Fragments arranged by size: 4334 2002 1900 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6044 276 Size: 53 83 186 936 4510 2468 Fragments arranged by size: 4510 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI Bsu36I EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI