(Circular) (Six-base) MAPSORT of: pFN21AB7351.seq Check: 9767 from: 1 to: 8473 pFN21AB7351 8473 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2590 2590 Size: 8473 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5355 6283 278 Size: 53 83 186 936 3819 928 2468 Fragments arranged by size: 3819 2468 936 928 186 83 53 AccI GT'mk_AC Cuts at: 1937 3047 4873 5759 5816 1937 Size: 1110 1826 886 57 4594 Fragments arranged by size: 4594 1826 1110 886 57 Acc65I G'GTAC_C Cuts at: 4771 5794 4771 Size: 1023 7450 AclI AA'CG_TT Cuts at: 1563 5680 6600 6973 1563 Size: 4117 920 373 3063 Fragments arranged by size: 4117 3063 920 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2718 2997 3836 3875 4274 4542 4571 4652 Size: 1086 279 839 39 399 268 29 81 Cuts at: 4652 4689 5649 6536 7920 8188 1632 Size: 37 960 887 1384 268 1917 Fragments arranged by size: 1917 1384 1086 960 887 839 399 279 268 268 81 39 37 29 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8276 AflIII A'CryG_T Cuts at: 1203 7372 8349 1203 Size: 6169 977 1327 Fragments arranged by size: 6169 1327 977 AgeI A'CCGG_T Cuts at: 7304 7304 Size: 8473 AhdI GACnn_n'nnGTC Cuts at: 7202 7202 Size: 8473 AleI CACnn'nnGTG Cuts at: 1166 4596 1166 Size: 3430 5043 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5211 1184 Size: 4027 4446 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3994 4026 3994 Size: 32 8441 AlwNI CAG_nnn'CTG Cuts at: 3356 5755 6216 7788 3356 Size: 2399 461 1572 4041 Fragments arranged by size: 4041 2399 1572 461 ApaLI G'TGCA_C Cuts at: 6530 7686 6530 Size: 1156 7317 ApoI r'AATT_y Cuts at: 1493 2132 2443 2698 3135 3841 4168 4318 4431 Size: 639 311 255 437 706 327 150 113 Cuts at: 4431 4911 5087 5537 5782 6008 6160 8269 1493 Size: 480 176 450 245 226 152 2109 1697 Fragments arranged by size: 2109 1697 706 639 480 450 437 327 311 255 245 226 176 152 150 113 AseI AT'TA_AT Cuts at: 160 1032 5556 7027 160 Size: 872 4524 1471 1606 Fragments arranged by size: 4524 1606 1471 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8473 AvaI C'yCGr_G Cuts at: 1870 1943 4254 5798 1870 Size: 73 2311 1544 4545 Fragments arranged by size: 4545 2311 1544 73 BamHI G'GATC_C Cuts at: 5101 5803 6170 5101 Size: 702 367 7404 Fragments arranged by size: 7404 702 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3409 4771 5195 5794 7249 Size: 325 217 627 1622 1362 424 599 1455 Cuts at: 7249 618 Size: 1842 Fragments arranged by size: 1842 1622 1455 1362 627 599 424 325 217 BanII G_rGCy'C Cuts at: 1391 2092 2622 4244 5646 5792 1391 Size: 701 530 1622 1402 146 4072 Fragments arranged by size: 4072 1622 1402 701 530 146 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2172 2352 3037 4996 5533 928 Size: 971 273 180 685 1959 537 3868 Fragments arranged by size: 3868 1959 971 685 537 273 180 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3905 3939 6664 6698 1903 Size: 34 50 34 1884 34 2725 34 3678 Fragments arranged by size: 3678 2725 1884 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6365 7575 6365 Size: 1210 7263 BclI T'GATC_A Cuts at: 1548 3829 1548 Size: 2281 6192 BfrBI ATG'CAT Cuts at: 3662 3662 Size: 8473 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2159 7084 136 Size: 107 122 71 631 605 487 4925 1525 Fragments arranged by size: 4925 1525 631 605 487 122 107 71 BglII A'GATC_T Cuts at: 8468 8468 Size: 8473 BlpI GC'TnA_GC Cuts at: 3300 5890 3300 Size: 2590 5883 Bme1580I G_kGCm'C Cuts at: 1790 4429 4759 6534 7690 1790 Size: 2639 330 1775 1156 2573 Fragments arranged by size: 2639 2573 1775 1156 330 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8473 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 6204 7162 Size: 416 53 83 399 24 2276 2511 958 Cuts at: 7162 442 Size: 1753 Fragments arranged by size: 2511 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8473 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2003 3147 3378 7133 1239 Size: 764 1144 231 3755 2579 Fragments arranged by size: 3755 2579 1144 764 231 Bpu10I CC'TnA_GC Cuts at: 2265 2517 4219 2265 Size: 252 1702 6519 Fragments arranged by size: 6519 1702 252 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6598 7463 7761 6598 Size: 865 298 7310 Fragments arranged by size: 7310 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5462 7136 882 Size: 617 3963 1674 2219 Fragments arranged by size: 3963 2219 1674 617 BsaAI yAC'GTr Cuts at: 493 1204 6264 493 Size: 711 5060 2702 Fragments arranged by size: 5060 2702 711 BsaBI GATnn'nnATC Cuts at: 2252 8467 2252 Size: 6215 2258 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4870 5352 6280 6662 Size: 53 83 186 936 3337 482 928 382 Cuts at: 6662 7297 275 Size: 635 1451 Fragments arranged by size: 3337 1451 936 928 635 482 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6906 7304 7578 7725 8136 6906 Size: 398 274 147 411 7243 Fragments arranged by size: 7243 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8248 Fragments arranged by size: 8248 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2789 4029 4493 4496 4820 1186 Size: 1603 1240 464 3 324 4839 Fragments arranged by size: 4839 1603 1240 464 324 3 BseYI C'CCAG_C Cuts at: 7676 8172 7676 Size: 496 7977 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4818 5367 5748 1163 Size: 573 3082 549 381 3888 Fragments arranged by size: 3888 3082 573 549 381 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5923 6684 7712 1540 Size: 275 186 3922 761 1028 2301 Fragments arranged by size: 3922 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2067 3163 3364 3760 4039 4244 6534 6619 7690 Size: 1096 201 396 279 205 2290 85 1071 Cuts at: 7690 2067 Size: 2850 Fragments arranged by size: 2850 2290 1096 1071 396 279 205 201 85 BsmI GAATG_Cn' Cuts at: 2898 5253 5526 5984 6077 2898 Size: 2355 273 458 93 5294 Fragments arranged by size: 5294 2355 458 273 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3313 3313 Size: 8473 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2092 2622 3163 3364 3760 4039 Size: 399 277 25 530 541 201 396 279 Cuts at: 4039 4244 4429 4759 5646 5792 6534 6619 7690 Size: 205 185 330 887 146 742 85 1071 Cuts at: 7690 1391 Size: 2174 Fragments arranged by size: 2174 1071 887 742 541 530 399 396 330 279 277 205 201 185 146 85 25 BspEI T'CCGG_A Cuts at: 8136 8136 Size: 8473 BspHI T'CATG_A Cuts at: 4735 5035 5598 6362 8092 4735 Size: 300 563 764 1730 5116 Fragments arranged by size: 5116 1730 764 563 300 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2590 4587 4783 5828 844 Size: 1746 1997 196 1045 3489 Fragments arranged by size: 3489 1997 1746 1045 196 BsrBI CCG'CTC Cuts at: 1394 1823 2017 2094 6360 1394 Size: 429 194 77 4266 3507 Fragments arranged by size: 4266 3507 429 194 77 BsrDI GCAATG_nn' Cuts at: 65 1248 6968 7142 65 Size: 1183 5720 174 1396 Fragments arranged by size: 5720 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7117 7304 1700 Size: 110 5307 187 2869 Fragments arranged by size: 5307 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8473 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8473 BssSI C'ACGA_G Cuts at: 5073 6533 7545 5073 Size: 1460 1012 6001 Fragments arranged by size: 6001 1460 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5146 5248 794 Size: 4352 102 4019 Fragments arranged by size: 4352 4019 102 BstBI TT'CG_AA Cuts at: 7277 7277 Size: 8473 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8473 BstYI r'GATC_y Cuts at: 1972 2467 2971 5101 5803 6170 6555 6572 8024 Size: 495 504 2130 702 367 385 17 1452 Cuts at: 8024 8110 8122 8468 1972 Size: 86 12 346 1977 Fragments arranged by size: 2130 1977 1452 702 504 495 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3048 3048 Size: 8473 Bsu36I CC'TnA_GG Cuts at: 2308 5001 2308 Size: 2693 5780 BtgI C'CryG_G Cuts at: 513 1065 4941 513 Size: 552 3876 4045 Fragments arranged by size: 4045 3876 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7734 Fragments arranged by size: 7734 683 56 BtsI GCAGTG_nn' Cuts at: 3556 5989 6783 6803 3556 Size: 2433 794 20 5226 Fragments arranged by size: 5226 2433 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8473 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8438 DraI TTT'AAA Cuts at: 5777 5777 Size: 8473 DraIII CAC_nnn'GTG Cuts at: 2071 5771 2071 Size: 3700 4773 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7480 809 Size: 507 6164 1802 Fragments arranged by size: 6164 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2160 5920 Size: 54 1425 50 275 18 195 135 3760 Cuts at: 5920 6809 8438 8 Size: 889 1629 43 Fragments arranged by size: 3760 1629 1425 889 275 195 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5920 1537 Size: 275 4108 4090 Fragments arranged by size: 4108 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2044 2136 2496 3420 4443 4497 4671 4797 Size: 691 92 360 924 1023 54 174 126 Cuts at: 4797 4857 5159 6403 1353 Size: 60 302 1244 3423 Fragments arranged by size: 3423 1244 1023 924 691 360 302 174 126 92 60 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5642 7060 7434 7580 387 Size: 1064 138 4053 1418 374 146 1280 Fragments arranged by size: 4053 1418 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4242 4242 Size: 8473 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2003 2718 2997 3147 3378 3836 3875 Size: 393 371 715 279 150 231 458 39 Cuts at: 3875 4274 4542 4571 4652 4689 5649 6536 7133 Size: 399 268 29 81 37 960 887 597 Cuts at: 7133 7920 8188 1239 Size: 787 268 1524 Fragments arranged by size: 1524 960 887 787 715 597 458 399 393 371 279 268 268 231 150 81 39 37 29 EcoNI CCTnn'n_nnAGG Cuts at: 3213 3213 Size: 8473 EcoO109I rG'GnC_Cy Cuts at: 2708 5752 2708 Size: 3044 5429 EcoRI G'AATT_C Cuts at: 4431 5782 6160 4431 Size: 1351 378 6744 Fragments arranged by size: 6744 1351 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4719 4719 Size: 8473 FspI TGC'GCA Cuts at: 3548 6979 3548 Size: 3431 5042 HaeII r_GCGC'y Cuts at: 1678 3567 7620 1678 Size: 1889 4053 2531 Fragments arranged by size: 4053 2531 1889 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2495 2527 3583 3615 5444 Size: 32 135 32 923 32 1056 32 1829 Cuts at: 5444 5476 5606 5638 7114 7146 7188 7220 1373 Size: 32 130 32 1476 32 42 32 2626 Fragments arranged by size: 2626 1829 1476 1056 923 135 130 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2291 4711 5206 5584 5760 5817 Size: 988 281 353 2420 495 378 176 57 Cuts at: 5817 6063 669 Size: 246 3079 Fragments arranged by size: 3079 2420 988 495 378 353 281 246 176 57 HindIII A'AGCT_T Cuts at: 748 2228 748 Size: 1480 6993 HpaI GTT'AAC Cuts at: 6063 6063 Size: 8473 KpnI G_GTAC'C Cuts at: 4775 5798 4775 Size: 1023 7450 MfeI C'AATT_G Cuts at: 6072 6072 Size: 8473 MluI A'CGCG_T Cuts at: 8349 8349 Size: 8473 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2160 2886 3267 3781 4304 7587 7771 1305 Size: 855 726 381 514 523 3283 184 2007 Fragments arranged by size: 3283 2007 855 726 523 514 381 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6651 Fragments arranged by size: 6651 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 4596 6431 6790 6949 518 Size: 648 1038 2392 1835 359 159 2042 Fragments arranged by size: 2392 2042 1835 1038 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 5698 5889 6566 7714 7959 1606 Size: 424 3668 191 677 1148 245 2120 Fragments arranged by size: 3668 2120 1148 677 424 245 191 NcoI C'CATG_G Cuts at: 513 1065 4941 513 Size: 552 3876 4045 Fragments arranged by size: 4045 3876 552 NdeI CA'TA_TG Cuts at: 387 2743 4132 4227 387 Size: 2356 1389 95 4633 Fragments arranged by size: 4633 2356 1389 95 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8473 NotI GC'GGCC_GC Cuts at: 5920 5920 Size: 8473 NsiI A_TGCA'T Cuts at: 3664 3664 Size: 8473 NspI r_CATG'y Cuts at: 1132 1229 5831 7376 1132 Size: 97 4602 1545 2229 Fragments arranged by size: 4602 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4014 1348 Size: 2666 5807 PciI A'CATG_T Cuts at: 7372 7372 Size: 8473 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8473 PfoI T'CCnGG_A Cuts at: 3680 5722 3680 Size: 2042 6431 PmeI GTTT'AAAC Cuts at: 5777 5777 Size: 8473 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6542 6574 8055 8087 6542 Size: 32 1481 32 6928 Fragments arranged by size: 6928 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2708 2708 Size: 8473 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8473 PsiI TTA'TAA Cuts at: 6043 6043 Size: 8473 PstI C_TGCA'G Cuts at: 830 3745 5825 830 Size: 2915 2080 3478 Fragments arranged by size: 3478 2915 2080 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8473 PvuII CAG'CTG Cuts at: 5698 5698 Size: 8473 RsrII CG'GwC_CG Cuts at: 2044 2044 Size: 8473 SacI G_AGCT'C Cuts at: 4244 4244 Size: 8473 SalI G'TCGA_C Cuts at: 1936 5758 5815 1936 Size: 3822 57 4594 Fragments arranged by size: 4594 3822 57 SapI GCTCTTCn'nnn_ Cuts at: 2136 4497 4671 2136 Size: 2361 174 5938 Fragments arranged by size: 5938 2361 174 SbfI CC_TGCA'GG Cuts at: 5825 5825 Size: 8473 ScaI AGT'ACT Cuts at: 2614 6721 8433 2614 Size: 4107 1712 2654 Fragments arranged by size: 4107 2654 1712 SfcI C'TryA_G Cuts at: 826 1045 3202 3741 4653 5631 5821 6956 7637 Size: 219 2157 539 912 978 190 1135 681 Cuts at: 7637 7828 826 Size: 191 1471 Fragments arranged by size: 2157 1471 1135 978 912 681 539 219 191 190 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8473 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8473 SmaI CCC'GGG Cuts at: 1872 5800 1872 Size: 3928 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6577 7478 7740 820 Size: 197 926 4634 901 262 1553 Fragments arranged by size: 4634 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6264 493 Size: 5771 2702 SpeI A'CTAG_T Cuts at: 152 5264 152 Size: 5112 3361 SphI G_CATG'C Cuts at: 1132 5831 1132 Size: 4699 3774 SspI AAT'ATT Cuts at: 5 52 6397 5 Size: 47 6345 2081 Fragments arranged by size: 6345 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8473 StyI C'CwwG_G Cuts at: 513 1065 3568 4941 5912 513 Size: 552 2503 1373 971 3074 Fragments arranged by size: 3074 2503 1373 971 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2032 3517 3782 6500 6517 6670 6855 Size: 1255 452 1485 265 2718 17 153 185 Cuts at: 6855 7300 325 Size: 445 1498 Fragments arranged by size: 2718 1498 1485 1255 452 445 265 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2612 6719 8431 Size: 274 80 33 51 1443 635 4107 1712 Cuts at: 8431 96 Size: 138 Fragments arranged by size: 4107 1712 1443 635 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8433 8465 8433 Size: 32 8441 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8473 XbaI T'CTAG_A Cuts at: 2059 4159 5809 2059 Size: 2100 1650 4723 Fragments arranged by size: 4723 2100 1650 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8473 XmaI C'CCGG_G Cuts at: 1870 5798 1870 Size: 3928 4545 XmnI GAAnn'nnTTC Cuts at: 2751 3034 3249 3689 5681 6602 2751 Size: 283 215 440 1992 921 4622 Fragments arranged by size: 4622 1992 921 440 283 215 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5353 6281 276 Size: 53 83 186 936 3819 928 2468 Fragments arranged by size: 3819 2468 936 928 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI