################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcyI AflIII AgeI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp700I AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 1260 GGTACC G^GTACC AflII 928 CTTAAG C^TTAAG AhdI 175 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1048 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 1260 GGTACC G^GTACC AspEI 175 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1088 GAGCTC G[AT]GC[AT]^C AvaII 1633 GGACC G^G[AT]CC BanI 1260 GGTACC G^G[CT][GA]CC BbsI 1639 GAAGAC GAAGAC(2/6) BbvCI 973 GCTGAGG CCTCAGC(-5/-2) BbvI 313 GCAGC GCAGC(8/12) BfrI 928 CTTAAG C^TTAAG BpuAI 1639 GAAGAC GAAGAC(2/6) BsaWI 6 TCCGGT [AT]^CCGG[AT] BsiHKAI 1088 GAGCTC G[AT]GC[AT]^C BspHI 53 TCATGA T^CATGA BssSI 1675 CACGAG CACGAG(-5/-1) BstAPI 1614 GCAGGTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraI 1443 TTTAAA TTT^AAA Eam1105I 175 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 1264 CCTTGG C^C[AT][AT]GG EcoT22I 112 ATGCAT ATGCA^T Fnu4HI 313 GCAGC GC^[ACGT]GC HapII 7 CCGG C^CGG HpaII 7 CCGG C^CGG ItaI 313 GCAGC GC^[ACGT]GC KpnI 1260 GGTACC GGTAC^C MaeII 938 ACGT A^CGT MaeIII 571 GTCAC ^GT[ACGT]AC MslI 93 CATAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 7 CCGG C^CGG NlaIV 1260 GGTACC GG[ACGT]^[ACGT]CC NsiI 112 ATGCAT ATGCA^T PpuMI 1632 AGGACCT [GA]G^G[AT]CC[CT] RcaI 53 TCATGA T^CATGA SacI 1088 GAGCTC GAGCT^C ScaI 1779 AGTACT AGT^ACT StyI 1264 CCTTGG C^C[AT][AT]GG TseI 313 GCAGC G^C[AT]GC Tsp45I 571 GTCAC ^GT[GC]AC TspRI 1569 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AseI 958 ATTAAT AT^TAAT 1594 ATTAAT AsnI 958 ATTAAT AT^TAAT 1594 ATTAAT BanII 997 GAGCCC G[GA]GC[CT]^C 1088 GAGCTC BmyI 997 GAGCCC G[AGT]GC[ACT]^C 1088 GAGCTC BpmI 1091 CTCCAG CTGGAG(16/14) 1645 CTCCAG BsaJI 23 CCCAGG C^C[ACGT][ACGT]GG 1264 CCTTGG BsmAI 198 GAGAC GTCTC(1/5) 132 GTCTC Bsp1286I 997 GAGCCC G[AGT]GC[ACT]^C 1088 GAGCTC BssKI 24 CCAGG ^CC[ACGT]GG 866 CCAGG BstNI 24 CCAGG CC^[AT]GG 866 CCAGG Cfr13I 1023 GGCCC G^G[ACGT]CC 1633 GGACC DraII 1022 AGGCCCT [GA]G^G[ACGT]CC[CT] 1632 AGGACCT EcoO109I 1022 AGGCCCT [GA]G^G[ACGT]CC[CT] 1632 AGGACCT EcoRII 24 CCAGG ^CC[AT]GG 866 CCAGG HincII 35 GTCAAC GT[CT]^[GA]AC 236 GTCAAC HindII 35 GTCAAC GT[CT]^[GA]AC 236 GTCAAC MvaI 24 CCAGG CC^[AT]GG 866 CCAGG PshBI 958 ATTAAT AT^TAAT 1594 ATTAAT PstI 1250 CTGCAG CTGCA^G 1760 CTGCAG Sau96I 1023 GGCCC G^G[ACGT]CC 1633 GGACC ScrFI 24 CCAGG CC^[ACGT]GG 866 CCAGG SmlI 796 CTCAAG C^T[CT][GA]AG 928 CTTAAG StuI 768 AGGCCT AGG^CCT 868 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AcsI AfaI AluI ApoI BfaI BglII BspMI BsrI BstF5I BstYI Cac8I DdeI DpnII FokI HaeIII HinfI HphI MaeI MboI MboII MflI MnlI MseI MwoI NdeII NlaIII PleI RsaI Sau3AI SfaNI SfcI TfiI Tru9I Tsp509I XhoII