(Circular) (Six-base) MAPSORT of: pFN21AE2183.seq Check: 4780 from: 1 to: 6496 pFN21AE2183 6496 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2899 2899 Size: 6496 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4306 278 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3839 1937 Size: 1902 4594 Acc65I G'GTAC_C Cuts at: 3265 3817 3265 Size: 552 5944 AclI AA'CG_TT Cuts at: 1563 4623 4996 1563 Size: 3060 373 3063 Fragments arranged by size: 3063 3060 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2282 3663 4559 5943 6211 1632 Size: 650 1381 896 1384 268 1917 Fragments arranged by size: 1917 1384 1381 896 650 268 AflII C'TTAA_G Cuts at: 820 1017 2933 820 Size: 197 1916 4383 Fragments arranged by size: 4383 1916 197 AflIII A'CryG_T Cuts at: 1203 5395 6372 1203 Size: 4192 977 1327 Fragments arranged by size: 4192 1327 977 AgeI A'CCGG_T Cuts at: 5327 5327 Size: 6496 AhdI GACnn_n'nnGTC Cuts at: 2185 5225 2185 Size: 3040 3456 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6496 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2120 1184 Size: 936 5560 AlwNI CAG_nnn'CTG Cuts at: 3058 4239 5811 3058 Size: 1181 1572 3743 Fragments arranged by size: 3743 1572 1181 ApaLI G'TGCA_C Cuts at: 4553 5709 4553 Size: 1156 5340 ApoI r'AATT_y Cuts at: 1493 2209 2682 3136 3686 3805 4031 4183 6292 Size: 716 473 454 550 119 226 152 2109 Cuts at: 6292 1493 Size: 1697 Fragments arranged by size: 2109 1697 716 550 473 454 226 152 119 AseI AT'TA_AT Cuts at: 160 1032 2964 3600 5050 160 Size: 872 1932 636 1450 1606 Fragments arranged by size: 1932 1606 1450 872 636 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 AvaI C'yCGr_G Cuts at: 1870 1943 3821 1870 Size: 73 1878 4545 Fragments arranged by size: 4545 1878 73 BamHI G'GATC_C Cuts at: 3826 4193 3826 Size: 367 6129 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3265 3817 5272 618 Size: 325 217 627 1478 552 1455 1842 Fragments arranged by size: 1842 1478 1455 627 552 325 217 BanII G_rGCy'C Cuts at: 1391 3006 3097 3815 1391 Size: 1615 91 718 4072 Fragments arranged by size: 4072 1615 718 91 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3651 928 Size: 971 1752 3773 Fragments arranged by size: 3773 1752 971 BbvCI CC'TCA_GC Cuts at: 2979 2979 Size: 6496 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4687 4721 1903 Size: 34 2750 34 3678 Fragments arranged by size: 3678 2750 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4388 5598 4388 Size: 1210 5286 BclI T'GATC_A Cuts at: 1548 1548 Size: 6496 BfrBI ATG'CAT Cuts at: 2119 2119 Size: 6496 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5107 136 Size: 107 122 71 631 605 3435 1525 Fragments arranged by size: 3435 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2155 2164 2440 3148 6491 2155 Size: 9 276 708 3343 2160 Fragments arranged by size: 3343 2160 708 276 9 BlpI GC'TnA_GC Cuts at: 3913 3913 Size: 6496 Bme1580I G_kGCm'C Cuts at: 1790 4557 5713 1790 Size: 2767 1156 2573 Fragments arranged by size: 2767 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6496 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4227 5185 442 Size: 416 53 83 399 24 2810 958 1753 Fragments arranged by size: 2810 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6496 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3081 3635 5156 1239 Size: 1842 554 1521 2579 Fragments arranged by size: 2579 1842 1521 554 Bpu10I CC'TnA_GC Cuts at: 2979 2979 Size: 6496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2786 4621 5486 5784 2786 Size: 1835 865 298 3498 Fragments arranged by size: 3498 1835 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5159 882 Size: 617 3660 2219 Fragments arranged by size: 3660 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4287 493 Size: 711 3083 2702 Fragments arranged by size: 3083 2702 711 BsaBI GATnn'nnATC Cuts at: 6490 6490 Size: 6496 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4303 4685 5320 275 Size: 53 83 186 936 2770 382 635 1451 Fragments arranged by size: 2770 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2011 4929 5327 5601 5748 6159 2011 Size: 2918 398 274 147 411 2348 Fragments arranged by size: 2918 2348 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6271 Fragments arranged by size: 6271 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6496 BseYI C'CCAG_C Cuts at: 3005 3694 5699 6195 3005 Size: 689 2005 496 3306 Fragments arranged by size: 3306 2005 689 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5923 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3946 4707 5735 1540 Size: 275 186 1945 761 1028 2301 Fragments arranged by size: 2301 1945 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3097 4557 4642 5713 3097 Size: 1460 85 1071 3880 Fragments arranged by size: 3880 1460 1071 85 BsmI GAATG_Cn' Cuts at: 4007 4100 4007 Size: 93 6403 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3006 3097 3815 4557 4642 5713 1391 Size: 399 1216 91 718 742 85 1071 2174 Fragments arranged by size: 2174 1216 1071 742 718 399 91 85 BspEI T'CCGG_A Cuts at: 6159 6159 Size: 6496 BspHI T'CATG_A Cuts at: 2058 4385 6115 2058 Size: 2327 1730 2439 Fragments arranged by size: 2439 2327 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2899 3610 3619 3772 3851 844 Size: 2055 711 9 153 79 3489 Fragments arranged by size: 3489 2055 711 153 79 9 BsrBI CCG'CTC Cuts at: 1394 1823 4383 1394 Size: 429 2560 3507 Fragments arranged by size: 3507 2560 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4991 5165 65 Size: 1183 3743 174 1396 Fragments arranged by size: 3743 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5140 5327 1700 Size: 110 3330 187 2869 Fragments arranged by size: 3330 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6496 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6496 BssSI C'ACGA_G Cuts at: 3680 4556 5568 3680 Size: 876 1012 4608 Fragments arranged by size: 4608 1012 876 BstAPI GCAn_nnn'nTGC Cuts at: 794 3625 794 Size: 2831 3665 BstBI TT'CG_AA Cuts at: 5300 5300 Size: 6496 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6496 BstYI r'GATC_y Cuts at: 1972 2155 2164 2440 3148 3826 4193 4578 4595 Size: 183 9 276 708 678 367 385 17 Cuts at: 4595 6047 6133 6145 6491 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 708 678 385 367 346 276 183 86 17 12 9 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5944 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5757 Fragments arranged by size: 5757 683 56 BtsI GCAGTG_nn' Cuts at: 4012 4806 4826 4012 Size: 794 20 5682 Fragments arranged by size: 5682 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6496 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6461 DraI TTT'AAA Cuts at: 3450 3800 3450 Size: 350 6146 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5503 809 Size: 507 4187 1802 Fragments arranged by size: 4187 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3943 4832 6461 Size: 54 1425 50 275 18 2113 889 1629 Cuts at: 6461 8 Size: 43 Fragments arranged by size: 2113 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3943 1537 Size: 275 2131 4090 Fragments arranged by size: 4090 2131 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4426 1353 Size: 3073 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5083 5457 5603 387 Size: 1064 138 3494 374 146 1280 Fragments arranged by size: 3494 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3095 3095 Size: 6496 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2282 3081 3635 3663 4559 5156 5943 Size: 393 650 799 554 28 896 597 787 Cuts at: 5943 6211 1239 Size: 268 1524 Fragments arranged by size: 1524 896 799 787 650 597 554 393 268 28 EcoO109I rG'GnC_Cy Cuts at: 3028 3638 3028 Size: 610 5886 EcoRI G'AATT_C Cuts at: 3805 4183 3805 Size: 378 6118 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2430 2430 Size: 6496 FspI TGC'GCA Cuts at: 5002 5002 Size: 6496 HaeII r_GCGC'y Cuts at: 1678 5643 1678 Size: 3965 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 2384 2416 3533 Size: 32 135 32 599 32 181 32 1117 Cuts at: 3533 3565 5137 5169 5211 5243 1373 Size: 32 1572 32 42 32 2626 Fragments arranged by size: 2626 1572 1117 599 181 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2042 2243 3840 4086 669 Size: 988 281 104 201 1597 246 3079 Fragments arranged by size: 3079 1597 988 281 246 201 104 HindIII A'AGCT_T Cuts at: 748 748 Size: 6496 HpaI GTT'AAC Cuts at: 4086 4086 Size: 6496 KpnI G_GTAC'C Cuts at: 3269 3821 3269 Size: 552 5944 MfeI C'AATT_G Cuts at: 4095 4095 Size: 6496 MluI A'CGCG_T Cuts at: 6372 6372 Size: 6496 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3559 5610 5794 1305 Size: 2254 2051 184 2007 Fragments arranged by size: 2254 2051 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4674 Fragments arranged by size: 4674 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2102 4454 4813 4972 518 Size: 648 936 2352 359 159 2042 Fragments arranged by size: 2352 2042 936 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3912 4589 5737 5982 1606 Size: 2306 677 1148 245 2120 Fragments arranged by size: 2306 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5944 NdeI CA'TA_TG Cuts at: 387 387 Size: 6496 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6496 NotI GC'GGCC_GC Cuts at: 3943 3943 Size: 6496 NsiI A_TGCA'T Cuts at: 2121 2121 Size: 6496 NspI r_CATG'y Cuts at: 1132 1229 3854 5399 1132 Size: 97 2625 1545 2229 Fragments arranged by size: 2625 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6496 PciI A'CATG_T Cuts at: 5395 5395 Size: 6496 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6496 PmeI GTTT'AAAC Cuts at: 3800 3800 Size: 6496 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4565 4597 6078 6110 4565 Size: 32 1481 32 4951 Fragments arranged by size: 4951 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3638 3638 Size: 6496 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6496 PsiI TTA'TAA Cuts at: 2487 2769 2901 3111 4066 2487 Size: 282 132 210 955 4917 Fragments arranged by size: 4917 955 282 210 132 PstI C_TGCA'G Cuts at: 830 3259 3769 3848 830 Size: 2429 510 79 3478 Fragments arranged by size: 3478 2429 510 79 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6496 SacI G_AGCT'C Cuts at: 3097 3097 Size: 6496 SalI G'TCGA_C Cuts at: 1936 3838 1936 Size: 1902 4594 SbfI CC_TGCA'GG Cuts at: 3848 3848 Size: 6496 ScaI AGT'ACT Cuts at: 3786 4744 6456 3786 Size: 958 1712 3826 Fragments arranged by size: 3826 1712 958 SfcI C'TryA_G Cuts at: 826 1045 2542 2740 3255 3589 3765 3844 4979 Size: 219 1497 198 515 334 176 79 1135 Cuts at: 4979 5660 5851 826 Size: 681 191 1471 Fragments arranged by size: 1497 1471 1135 681 515 334 219 198 191 176 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6496 SmaI CCC'GGG Cuts at: 1872 3823 1872 Size: 1951 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2801 2933 4600 5501 5763 820 Size: 197 926 858 132 1667 901 262 1553 Fragments arranged by size: 1667 1553 926 901 858 262 197 132 SnaBI TAC'GTA Cuts at: 493 4287 493 Size: 3794 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6496 SphI G_CATG'C Cuts at: 1132 3854 1132 Size: 2722 3774 SspI AAT'ATT Cuts at: 5 52 4420 5 Size: 47 4368 2081 Fragments arranged by size: 4368 2081 47 StuI AGG'CCT Cuts at: 1517 2775 2875 1517 Size: 1258 100 5138 Fragments arranged by size: 5138 1258 100 StyI C'CwwG_G Cuts at: 513 1065 3269 3935 513 Size: 552 2204 666 3074 Fragments arranged by size: 3074 2204 666 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4523 4540 4693 4878 5323 325 Size: 1255 2943 17 153 185 445 1498 Fragments arranged by size: 2943 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3784 4742 6454 Size: 274 80 33 51 1443 1807 958 1712 Cuts at: 6454 96 Size: 138 Fragments arranged by size: 1807 1712 1443 958 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6456 6488 6456 Size: 32 6464 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6496 XbaI T'CTAG_A Cuts at: 3832 3832 Size: 6496 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6496 XmaI C'CCGG_G Cuts at: 1870 3821 1870 Size: 1951 4545 XmnI GAAnn'nnTTC Cuts at: 4625 4625 Size: 6496 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4304 276 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI