################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DrdI DsaI Eam1105I EarI Eco47III EcoNI EcoO65I EcoRV EcoT14I EcoT22I FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MamI MboII MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 61 GTCTAC GT^[AC][GT]AC AlwNI 981 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 15 CCTGAGG CC^T[ACGT]AGG AspHI 426 GAGCTC G[AT]GC[AT]^C BanII 426 GAGCTC G[GA]GC[CT]^C BbvCI 181 GCTGAGG CCTCAGC(-5/-2) BclI 368 TGATCA T^GATCA BfaI 1102 CTAG C^TAG BmyI 426 GAGCTC G[AGT]GC[ACT]^C BsiEI 8 CGGCCG CG[GA][CT]^CG BsiHKAI 426 GAGCTC G[AT]GC[AT]^C BsmAI 528 GAGAC GTCTC(1/5) BsmI 556 GAATGC GAATGC(1/-1) Bsp1286I 426 GAGCTC G[AGT]GC[ACT]^C BspHI 851 TCATGA T^CATGA BsrBI 26 CCGCTC CCGCTC(-3/-3) BstF5I 51 GGATG GGATG(2/0) Bsu36I 15 CCTGAGG CC^T[ACGT]AGG DpnII 369 GATC ^GATC DraI 1357 TTTAAA TTT^AAA DraIII 391 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 8 CGGCCG [CT]^GGCC[GA] EagI 8 CGGCCG C^GGCCG EclXI 8 CGGCCG C^GGCCG Eco52I 8 CGGCCG C^GGCCG Eco81I 15 CCTGAGG CC^T[ACGT]AGG FauI 25 CCCGC CCCGC(4/6) FbaI 368 TGATCA T^GATCA FokI 51 GGATG GGATG(9/13) HgaI 97 GACGC GACGC(5/10) HindIII 743 AAGCTT A^AGCTT MaeI 1102 CTAG C^TAG MaeII 550 ACGT A^CGT MboI 369 GATC ^GATC MslI 943 CATTGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 369 GATC ^GATC NotI 7 GCGGCCGC GC^GGCCGC PleI 399 GACTC GAGTC(4/5) RcaI 851 TCATGA T^CATGA SacI 426 GAGCTC GAGCT^C Sau3AI 369 GATC ^GATC SexAI 392 ACCAGGT A^CC[AT]GGT SspI 506 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AvaII 21 GGACC G^G[AT]CC 291 GGTCC BbvI 249 GCAGC GCAGC(8/12) 980 GCAGC BsaJI 71 CCCAGG C^C[ACGT][ACGT]GG 176 CCGAGG BsmFI 20 GGGAC GGGAC(10/14) 292 GTCCC BssKI 72 CCAGG ^CC[ACGT]GG 393 CCAGG BstNI 72 CCAGG CC^[AT]GG 393 CCAGG EcoRI 1092 GAATTC G^AATTC 1260 GAATTC EcoRII 72 CCAGG ^CC[AT]GG 393 CCAGG MvaI 72 CCAGG CC^[AT]GG 393 CCAGG NspI 534 ACATGC [GA]CATG^[CT] 724 ACATGC PpuMI 20 GGGACCC [GA]G^G[AT]CC[CT] 290 GGGTCCC ScrFI 72 CCAGG CC^[ACGT]GG 393 CCAGG SfaNI 126 GATGC GCATC(5/9) 774 GCATC SfcI 209 CTGTAG C^T[GA][CT]AG 915 CTATAG SmlI 385 CTCAAG C^T[CT][GA]AG 1011 CTCAAG TaqI 1197 TCGA T^CGA 1365 TCGA TfiI 1079 GATTC G^A[AT]TC 1371 GATTC TseI 249 GCAGC G^C[AT]GC 980 GCAGC TthHB8I 1197 TCGA T^CGA 1365 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AcsI AluI ApoI BpmI BseRI BsgI BsiYI BslI BsrDI BsrI Cfr13I DdeI DraII EcoO109I Fnu4HI HaeIII HinfI HphI ItaI MaeIII MnlI MseI MwoI NlaIII NlaIV Sau96I StuI Tru9I Tsp45I Tsp509I TspRI