(Circular) (Six-base) MAPSORT of: pFN21AE1156.seq Check: 3465 from: 1 to: 6097 pFN21AE1156 6097 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2067 3440 1937 Size: 130 1373 4594 Fragments arranged by size: 4594 1373 130 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4160 5544 5812 1632 Size: 2528 1384 268 1917 Fragments arranged by size: 2528 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 4996 5973 1203 Size: 3793 977 1327 Fragments arranged by size: 3793 1327 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6097 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2147 1184 Size: 963 5134 AlwNI CAG_nnn'CTG Cuts at: 2991 3840 5412 2991 Size: 849 1572 3676 Fragments arranged by size: 3676 1572 849 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 2915 2999 3097 3167 3265 3406 3632 3784 Size: 1422 84 98 70 98 141 226 152 Cuts at: 3784 5893 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1422 226 152 141 98 98 84 70 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 3422 1870 Size: 73 1479 4545 Fragments arranged by size: 4545 1479 73 BamHI G'GATC_C Cuts at: 3427 3794 3427 Size: 367 5730 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3418 4873 618 Size: 325 217 627 1631 1455 1842 Fragments arranged by size: 1842 1631 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2435 3416 1391 Size: 1044 981 4072 Fragments arranged by size: 4072 1044 981 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5126 BbvCI CC'TCA_GC Cuts at: 2187 2187 Size: 6097 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4288 4322 1903 Size: 34 2351 34 3678 Fragments arranged by size: 3678 2351 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3989 5199 3989 Size: 1210 4887 BclI T'GATC_A Cuts at: 1548 2373 1548 Size: 825 5272 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4708 136 Size: 107 122 71 631 605 337 2699 1525 Fragments arranged by size: 2699 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6092 6092 Size: 6097 BlpI GC'TnA_GC Cuts at: 3514 3514 Size: 6097 Bme1580I G_kGCm'C Cuts at: 1790 4158 5314 1790 Size: 2368 1156 2573 Fragments arranged by size: 2573 2368 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6097 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2294 3828 4786 Size: 416 53 83 399 24 877 1534 958 Cuts at: 4786 442 Size: 1753 Fragments arranged by size: 1753 1534 958 877 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2649 2733 2817 2833 2901 2985 3069 Size: 917 493 84 84 16 68 84 84 Cuts at: 3069 3153 3321 3337 3405 4757 1239 Size: 84 168 16 68 1352 2579 Fragments arranged by size: 2579 1352 917 493 168 84 84 84 84 84 68 68 16 16 Bpu10I CC'TnA_GC Cuts at: 2034 2187 2199 2380 2034 Size: 153 12 181 5751 Fragments arranged by size: 5751 181 153 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2375 3001 4222 5087 5385 2375 Size: 626 1221 865 298 3087 Fragments arranged by size: 3087 1221 865 626 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4760 882 Size: 617 3261 2219 Fragments arranged by size: 3261 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3888 493 Size: 711 2684 2702 Fragments arranged by size: 2702 2684 711 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3904 4286 4921 275 Size: 53 83 186 936 2371 382 635 1451 Fragments arranged by size: 2371 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4530 4928 5202 5349 5760 4530 Size: 398 274 147 411 4867 Fragments arranged by size: 4867 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5872 Fragments arranged by size: 5872 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2204 2323 1186 Size: 919 99 119 4960 Fragments arranged by size: 4960 919 119 99 BseYI C'CCAG_C Cuts at: 2543 5300 5796 2543 Size: 2757 496 2844 Fragments arranged by size: 2844 2757 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2836 2920 3004 3172 3340 1163 Size: 573 1100 84 84 168 168 3920 Fragments arranged by size: 3920 1100 573 168 168 84 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 3547 4308 5336 1540 Size: 275 186 15 1531 761 1028 2301 Fragments arranged by size: 2301 1531 1028 761 275 186 15 BsiHKAI G_wGCw'C Cuts at: 2435 4158 4243 5314 2435 Size: 1723 85 1071 3218 Fragments arranged by size: 3218 1723 1071 85 BsmI GAATG_Cn' Cuts at: 2567 3608 3701 2567 Size: 1041 93 4963 Fragments arranged by size: 4963 1041 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2435 3416 4158 4243 5314 1391 Size: 399 645 981 742 85 1071 2174 Fragments arranged by size: 2174 1071 981 742 645 399 85 BspEI T'CCGG_A Cuts at: 5760 5760 Size: 6097 BspHI T'CATG_A Cuts at: 2856 3986 5716 2856 Size: 1130 1730 3237 Fragments arranged by size: 3237 1730 1130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3385 3452 844 Size: 2541 67 3489 Fragments arranged by size: 3489 2541 67 BsrBI CCG'CTC Cuts at: 1394 1823 2033 3984 1394 Size: 429 210 1951 3507 Fragments arranged by size: 3507 1951 429 210 BsrDI GCAATG_nn' Cuts at: 65 1248 2131 3076 3244 4592 4766 65 Size: 1183 883 945 168 1348 174 1396 Fragments arranged by size: 1396 1348 1183 945 883 174 168 BsrFI r'CCGG_y Cuts at: 1700 1810 4741 4928 1700 Size: 110 2931 187 2869 Fragments arranged by size: 2931 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6097 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6097 BstYI r'GATC_y Cuts at: 1972 3427 3794 4179 4196 5648 5734 5746 6092 Size: 1455 367 385 17 1452 86 12 346 Cuts at: 6092 1972 Size: 1977 Fragments arranged by size: 1977 1455 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2021 2021 Size: 6097 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4606 Fragments arranged by size: 4606 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 2488 2572 2740 2824 2908 3613 4407 4427 2488 Size: 84 168 84 84 705 794 20 4158 Fragments arranged by size: 4158 794 705 168 84 84 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3058 3093 3226 3261 529 Size: 35 2494 35 133 35 3365 Fragments arranged by size: 3365 2494 133 35 35 35 DraI TTT'AAA Cuts at: 3364 3401 3364 Size: 37 6060 DraIII CAC_nnn'GTG Cuts at: 2401 2401 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5104 809 Size: 507 3788 1802 Fragments arranged by size: 3788 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 3544 4433 Size: 54 1425 50 275 18 183 1531 889 Cuts at: 4433 6062 8 Size: 1629 43 Fragments arranged by size: 1629 1531 1425 889 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 3544 1537 Size: 275 201 1531 4090 Fragments arranged by size: 4090 1531 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 4027 1353 Size: 2674 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4684 5058 5204 387 Size: 1064 138 3095 374 146 1280 Fragments arranged by size: 3095 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2433 2433 Size: 6097 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2649 2733 2817 2833 2901 2985 Size: 393 524 493 84 84 16 68 84 Cuts at: 2985 3069 3153 3321 3337 3405 4160 4757 5544 Size: 84 84 168 16 68 755 597 787 Cuts at: 5544 5812 1239 Size: 268 1524 Fragments arranged by size: 1524 787 755 597 524 493 393 268 168 84 84 84 84 84 68 68 16 16 EcoO109I rG'GnC_Cy Cuts at: 2026 2296 2365 2026 Size: 270 69 5758 Fragments arranged by size: 5758 270 69 EcoRI G'AATT_C Cuts at: 3097 3265 3406 3784 3097 Size: 168 141 378 5410 Fragments arranged by size: 5410 378 168 141 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 5244 1678 Size: 3566 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4738 4770 4812 4844 1373 Size: 32 135 32 3166 32 42 32 2626 Fragments arranged by size: 3166 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3441 3687 669 Size: 988 281 1503 246 3079 Fragments arranged by size: 3079 1503 988 281 246 HindIII A'AGCT_T Cuts at: 748 2748 748 Size: 2000 4097 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2622 2958 5211 5395 1305 Size: 1317 336 2253 184 2007 Fragments arranged by size: 2253 2007 1317 336 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4275 Fragments arranged by size: 4275 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2952 4055 4414 4573 518 Size: 648 1786 1103 359 159 2042 Fragments arranged by size: 2042 1786 1103 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3513 4190 5338 5583 1606 Size: 1907 677 1148 245 2120 Fragments arranged by size: 2120 1907 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4606 Fragments arranged by size: 4606 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 2013 3544 2013 Size: 1531 4566 NspI r_CATG'y Cuts at: 1132 1229 2543 2733 3455 5000 1132 Size: 97 1314 190 722 1545 2229 Fragments arranged by size: 2229 1545 1314 722 190 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6097 PciI A'CATG_T Cuts at: 4996 4996 Size: 6097 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6097 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4166 4198 5679 5711 4166 Size: 32 1481 32 4552 Fragments arranged by size: 4552 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2026 2296 2026 Size: 270 5827 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 2895 3667 2895 Size: 772 5325 PstI C_TGCA'G Cuts at: 830 3449 830 Size: 2619 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 SacI G_AGCT'C Cuts at: 2435 2435 Size: 6097 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SanDI GG'GwC_CC Cuts at: 2026 2296 2026 Size: 270 5827 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SexAI A'CCwGG_T Cuts at: 2397 2397 Size: 6097 SfcI C'TryA_G Cuts at: 826 1045 2214 2920 3445 4580 5261 5452 826 Size: 219 1169 706 525 1135 681 191 1471 Fragments arranged by size: 1471 1169 1135 706 681 525 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 3424 1872 Size: 1552 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2390 3016 4201 5102 5364 820 Size: 197 926 447 626 1185 901 262 1553 Fragments arranged by size: 1553 1185 926 901 626 447 262 197 SnaBI TAC'GTA Cuts at: 493 3888 493 Size: 3395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 3455 1132 Size: 2323 3774 SspI AAT'ATT Cuts at: 5 52 2513 4021 5 Size: 47 2461 1508 2081 Fragments arranged by size: 2461 2081 1508 47 StuI AGG'CCT Cuts at: 1517 2450 2638 2806 2974 1517 Size: 933 188 168 168 4640 Fragments arranged by size: 4640 933 188 168 168 StyI C'CwwG_G Cuts at: 513 1065 2004 3536 513 Size: 552 939 1532 3074 Fragments arranged by size: 3074 1532 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4124 4141 4294 4479 4924 325 Size: 1255 2544 17 153 185 445 1498 Fragments arranged by size: 2544 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4343 6055 96 Size: 274 80 33 51 1443 2366 1712 138 Fragments arranged by size: 2366 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6057 6089 6057 Size: 32 6065 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6097 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 3422 1870 Size: 1552 4545 XmnI GAAnn'nnTTC Cuts at: 4226 4226 Size: 6097 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI