################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI DraI DraIII DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuII RcaI RsaI SalI SapI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Aor51HI 74 AGCGCT AGC^GCT AspHI 952 GAGCTC G[AT]GC[AT]^C AviII 878 TGCGCA TGC^GCA BcgI 294 CGAGCTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 525 GCTCAGC GC^T[ACGT]AGC Bpu1102I 525 GCTCAGC GC^T[ACGT]AGC BseRI 724 CTCCTC GAGGAG(10/8) BsiHKAI 952 GAGCTC G[AT]GC[AT]^C BspMI 817 ACCTGC ACCTGC(4/8) BsrFI 986 ACCGGC [GA]^CCGG[CT] BstXI 149 CCATGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 525 GCTCAGC GC^T[ACGT]AGC Cfr10I 986 ACCGGC [GA]^CCGG[CT] CpoI 561 CGGTCCG CG^G[AT]CCG EarI 661 CTCTTC CTCTTC(1/4) Eco47III 74 AGCGCT AGC^GCT EcoNI 357 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 149 CCATGG C^C[AT][AT]GG FspI 878 TGCGCA TGC^GCA HinfI 97 GAGTC G^A[ACGT]TC Ksp632I 661 CTCTTC CTCTTC(1/4) MboII 662 TCTTC GAAGA(8/7) NcoI 149 CCATGG C^CATGG NspI 476 GCATGC [GA]CATG^[CT] PleI 97 GAGTC GAGTC(4/5) PpuMI 483 AGGTCCC [GA]G^G[AT]CC[CT] PvuI 1011 CGATCG CGAT^CG RsrII 561 CGGTCCG CG^G[AT]CCG SacI 952 GAGCTC GAGCT^C SbfI 66 CCTGCAGG CCTGCA^GG SmlI 439 CTCAAG C^T[CT][GA]AG SphI 476 GCATGC GCATG^C Sse8387I 66 CCTGCAGG CCTGCA^GG StyI 149 CCATGG C^C[AT][AT]GG TaqI 665 TCGA T^CGA TthHB8I 665 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AscI 235 GGCGCGCC GG^CGCGCC 607 GGCGCGCC BpmI 748 CTGGAG CTGGAG(16/14) 757 CTGGAG BsaI 658 GGTCTC GGTCTC(1/5) 721 GGTCTC BsgI 322 CTGCAC GTGCAG(16/14) 1045 CTGCAC BsmAI 659 GTCTC GTCTC(1/5) 722 GTCTC BsrBI 523 CCGCTC CCGCTC(-3/-3) 783 CCGCTC BsrI 914 ACTGG ACTGG(1/-1) 996 CCAGT DpnII 1012 GATC ^GATC 1033 GATC DraII 312 GGGGCCC [GA]G^G[ACGT]CC[CT] 483 AGGTCCC EcoO109I 312 GGGGCCC [GA]G^G[ACGT]CC[CT] 483 AGGTCCC HgaI 670 GACGC GACGC(5/10) 739 GCGTC HphI 892 GGTGA GGTGA(8/7) 1035 TCACC MboI 1012 GATC ^GATC 1033 GATC MslI 886 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 992 CATTCCAGTG NdeII 1012 GATC ^GATC 1033 GATC NotI 673 GCGGCCGC GC^GGCCGC 776 GCGGCCGC PstI 67 CTGCAG CTGCA^G 819 CTGCAG Sau3AI 1012 GATC ^GATC 1033 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DsaI EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI KspI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV SacII Sau96I ScrFI SfaNI SfcI SfoI SmaI TseI TspRI XmaCI XmaI