(Circular) (Six-base) MAPSORT of: pFN21AB7604.seq Check: 1232 from: 1 to: 5770 pFN21AB7604 5770 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2831 2831 Size: 5770 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3580 278 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3113 1937 Size: 1176 4594 Acc65I G'GTAC_C Cuts at: 3091 3091 Size: 5770 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2403 2891 3833 5217 5485 1632 Size: 771 488 942 1384 268 1917 Fragments arranged by size: 1917 1384 942 771 488 268 AfeI AGC'GCT Cuts at: 2081 2081 Size: 5770 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 4669 5646 1203 Size: 3466 977 1327 Fragments arranged by size: 3466 1327 977 AgeI A'CCGG_T Cuts at: 4601 4601 Size: 5770 AhdI GACnn_n'nnGTC Cuts at: 4499 4499 Size: 5770 AleI CACnn'nnGTG Cuts at: 1166 2895 1166 Size: 1729 4041 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5770 AlwNI CAG_nnn'CTG Cuts at: 3513 5085 3513 Size: 1572 4198 ApaI G_GGCC'C Cuts at: 2322 2468 2625 2322 Size: 146 157 5467 Fragments arranged by size: 5467 157 146 ApaLI G'TGCA_C Cuts at: 3827 4983 3827 Size: 1156 4614 ApoI r'AATT_y Cuts at: 1493 3079 3305 3457 5566 1493 Size: 1586 226 152 2109 1697 Fragments arranged by size: 2109 1697 1586 226 152 AscI GG'CGCG_CC Cuts at: 2241 2613 2241 Size: 372 5398 AseI AT'TA_AT Cuts at: 160 1032 4324 160 Size: 872 3292 1606 Fragments arranged by size: 3292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 2099 2297 2417 2461 2492 2618 3095 Size: 73 156 198 120 44 31 126 477 Cuts at: 3095 1870 Size: 4545 Fragments arranged by size: 4545 477 198 156 126 120 73 44 31 BamHI G'GATC_C Cuts at: 3100 3467 3100 Size: 367 5403 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 2174 2347 2456 2707 Size: 325 217 627 324 63 173 109 251 Cuts at: 2707 3091 4546 618 Size: 384 1455 1842 Fragments arranged by size: 1842 1455 627 384 325 324 251 217 173 109 63 BanII G_rGCy'C Cuts at: 1391 2322 2379 2468 2625 2961 3089 1391 Size: 931 57 89 157 336 128 4072 Fragments arranged by size: 4072 931 336 157 128 89 57 BbeI G_GCGC'C Cuts at: 2178 2351 2460 2711 2178 Size: 173 109 251 5237 Fragments arranged by size: 5237 251 173 109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4799 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2288 2322 3961 3995 1903 Size: 34 351 34 1639 34 3678 Fragments arranged by size: 3678 1639 351 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3662 4872 3662 Size: 1210 4560 BclI T'GATC_A Cuts at: 1548 1548 Size: 5770 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2317 2541 2690 Size: 107 122 71 631 605 645 224 149 Cuts at: 2690 4381 136 Size: 1691 1525 Fragments arranged by size: 1691 1525 645 631 605 224 149 122 107 71 BglII A'GATC_T Cuts at: 5765 5765 Size: 5770 BlpI GC'TnA_GC Cuts at: 2531 3187 2531 Size: 656 5114 Bme1580I G_kGCm'C Cuts at: 1790 2322 2468 2625 3831 4987 1790 Size: 532 146 157 1206 1156 2573 Fragments arranged by size: 2573 1206 1156 532 157 146 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2929 3501 4459 Size: 416 53 83 399 24 1512 572 958 Cuts at: 4459 442 Size: 1753 Fragments arranged by size: 1753 1512 958 572 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2774 2783 4430 1239 Size: 1535 9 1647 2579 Fragments arranged by size: 2579 1647 1535 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2429 3895 4760 5058 2429 Size: 1466 865 298 3141 Fragments arranged by size: 3141 1466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2669 2732 4433 882 Size: 617 1170 63 1701 2219 Fragments arranged by size: 2219 1701 1170 617 63 BsaAI yAC'GTr Cuts at: 493 1204 3561 493 Size: 711 2357 2702 Fragments arranged by size: 2702 2357 711 BsaBI GATnn'nnATC Cuts at: 5764 5764 Size: 5770 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2175 2348 2457 2708 Size: 53 83 186 936 642 173 109 251 Cuts at: 2708 3577 3959 4594 275 Size: 869 382 635 1451 Fragments arranged by size: 1451 936 869 642 635 382 251 186 173 109 83 53 BsaWI w'CCGG_w Cuts at: 4203 4601 4875 5022 5433 4203 Size: 398 274 147 411 4540 Fragments arranged by size: 4540 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5545 Fragments arranged by size: 5545 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2720 1186 Size: 1534 4236 BseYI C'CCAG_C Cuts at: 2653 4973 5469 2653 Size: 2320 496 2954 Fragments arranged by size: 2954 2320 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2312 3035 1163 Size: 573 576 723 3898 Fragments arranged by size: 3898 723 576 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2335 2359 2682 2785 3019 3220 Size: 275 186 334 24 323 103 234 201 Cuts at: 3220 3981 5009 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 334 323 275 234 201 186 103 24 BsiHKAI G_wGCw'C Cuts at: 2961 3831 3916 4987 2961 Size: 870 85 1071 3744 Fragments arranged by size: 3744 1071 870 85 BsmI GAATG_Cn' Cuts at: 3281 3374 3281 Size: 93 5677 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2322 2379 2468 2625 2961 3089 3831 Size: 399 532 57 89 157 336 128 742 Cuts at: 3831 3916 4987 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 532 399 336 157 128 89 85 57 BspEI T'CCGG_A Cuts at: 5433 5433 Size: 5770 BspHI T'CATG_A Cuts at: 3659 5389 3659 Size: 1730 4040 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2831 3125 844 Size: 1987 294 3489 Fragments arranged by size: 3489 1987 294 BsrBI CCG'CTC Cuts at: 1394 1823 2530 2790 3657 1394 Size: 429 707 260 867 3507 Fragments arranged by size: 3507 867 707 429 260 BsrDI GCAATG_nn' Cuts at: 65 1248 4265 4439 65 Size: 1183 3017 174 1396 Fragments arranged by size: 3017 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2991 4414 4601 1700 Size: 110 1181 1423 187 2869 Fragments arranged by size: 2869 1423 1181 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5770 BssHII G'CGCG_C Cuts at: 1925 2089 2241 2549 2572 2613 2945 1925 Size: 164 152 308 23 41 332 4750 Fragments arranged by size: 4750 332 308 164 152 41 23 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5770 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5770 BstXI CCAn_nnnn'nTGG Cuts at: 2161 2161 Size: 5770 BstYI r'GATC_y Cuts at: 1972 3100 3467 3852 3869 5321 5407 5419 5765 Size: 1128 367 385 17 1452 86 12 346 Cuts at: 5765 1972 Size: 1977 Fragments arranged by size: 1977 1452 1128 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2154 2265 2428 2688 2722 513 Size: 552 939 150 111 163 260 34 3561 Fragments arranged by size: 3561 939 552 260 163 150 111 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5031 Fragments arranged by size: 5031 683 56 BtsI GCAGTG_nn' Cuts at: 2324 2911 3286 4080 4100 2324 Size: 587 375 794 20 3994 Fragments arranged by size: 3994 794 587 375 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5735 DraI TTT'AAA Cuts at: 3074 3074 Size: 5770 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4777 809 Size: 507 3461 1802 Fragments arranged by size: 3461 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2084 2332 2356 Size: 54 1425 50 275 18 254 248 24 Cuts at: 2356 2679 2782 2993 3217 4106 5735 8 Size: 323 103 211 224 889 1629 43 Fragments arranged by size: 1629 1425 889 323 275 254 248 224 211 103 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2332 2356 2679 2782 3217 1537 Size: 275 520 24 323 103 435 4090 Fragments arranged by size: 4090 520 435 323 275 103 24 EarI CTCTTCn'nnn_ Cuts at: 1353 2672 3700 1353 Size: 1319 1028 3423 Fragments arranged by size: 3423 1319 1028 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4357 4731 4877 387 Size: 1064 138 2768 374 146 1280 Fragments arranged by size: 2768 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2959 2959 Size: 5770 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2403 2774 2783 2891 3833 4430 5217 Size: 393 771 371 9 108 942 597 787 Cuts at: 5217 5485 1239 Size: 268 1524 Fragments arranged by size: 1524 942 787 771 597 393 371 268 108 9 EcoNI CCTnn'n_nnAGG Cuts at: 2366 2366 Size: 5770 EcoO109I rG'GnC_Cy Cuts at: 2318 2489 2318 Size: 171 5599 EcoRI G'AATT_C Cuts at: 3079 3457 3079 Size: 378 5392 FspI TGC'GCA Cuts at: 2885 4276 2885 Size: 1391 4379 HaeII r_GCGC'y Cuts at: 1678 2012 2083 2178 2262 2351 2460 2711 3050 Size: 334 71 95 84 89 109 251 339 Cuts at: 3050 4917 1678 Size: 1867 2531 Fragments arranged by size: 2531 1867 339 334 251 109 95 89 84 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2851 2883 4411 4443 4485 Size: 32 135 32 1279 32 1528 32 42 Cuts at: 4485 4517 1373 Size: 32 2626 Fragments arranged by size: 2626 1528 1279 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3114 3360 669 Size: 988 281 1176 246 3079 Fragments arranged by size: 3079 1176 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5770 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KasI G'GCGC_C Cuts at: 2174 2347 2456 2707 2174 Size: 173 109 251 5237 Fragments arranged by size: 5237 251 173 109 KpnI G_GTAC'C Cuts at: 3095 3095 Size: 5770 MfeI C'AATT_G Cuts at: 3369 3369 Size: 5770 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4884 5068 1305 Size: 3579 184 2007 Fragments arranged by size: 3579 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3948 Fragments arranged by size: 3948 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2895 3001 3728 4087 4246 518 Size: 648 1729 106 727 359 159 2042 Fragments arranged by size: 2042 1729 727 648 359 159 106 MspA1I CmG'CkG Cuts at: 1606 2267 2690 2724 2752 3186 3863 5011 5256 Size: 661 423 34 28 434 677 1148 245 Cuts at: 5256 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 661 434 423 245 34 28 NarI GG'CG_CC Cuts at: 2175 2348 2457 2708 2175 Size: 173 109 251 5237 Fragments arranged by size: 5237 251 173 109 NcoI C'CATG_G Cuts at: 513 1065 2004 2154 513 Size: 552 939 150 4129 Fragments arranged by size: 4129 939 552 150 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5770 NotI GC'GGCC_GC Cuts at: 2679 2782 3217 2679 Size: 103 435 5232 Fragments arranged by size: 5232 435 103 NspI r_CATG'y Cuts at: 1132 1229 2485 3128 4673 1132 Size: 97 1256 643 1545 2229 Fragments arranged by size: 2229 1545 1256 643 97 PasI CC'CwG_GG Cuts at: 1348 2698 1348 Size: 1350 4420 PciI A'CATG_T Cuts at: 4669 4669 Size: 5770 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5770 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2489 2489 Size: 5770 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5770 PsiI TTA'TAA Cuts at: 3340 3340 Size: 5770 PspOMI G'GGCC_C Cuts at: 2318 2464 2621 2318 Size: 146 157 5467 Fragments arranged by size: 5467 157 146 PstI C_TGCA'G Cuts at: 830 2076 2828 3122 830 Size: 1246 752 294 3478 Fragments arranged by size: 3478 1246 752 294 PvuI CG_AT'CG Cuts at: 2001 3019 2001 Size: 1018 4752 RsrII CG'GwC_CG Cuts at: 2567 2567 Size: 5770 SacI G_AGCT'C Cuts at: 2961 2961 Size: 5770 SacII CC_GC'GG Cuts at: 2268 2691 2725 2268 Size: 423 34 5313 Fragments arranged by size: 5313 423 34 SalI G'TCGA_C Cuts at: 1936 3112 1936 Size: 1176 4594 SbfI CC_TGCA'GG Cuts at: 2076 3122 2076 Size: 1046 4724 ScaI AGT'ACT Cuts at: 4018 5730 4018 Size: 1712 4058 SfcI C'TryA_G Cuts at: 826 1045 2072 2254 2824 3118 4253 4934 5125 Size: 219 1027 182 570 294 1135 681 191 Cuts at: 5125 826 Size: 1471 Fragments arranged by size: 1471 1135 1027 681 570 294 219 191 182 SfoI GGC'GCC Cuts at: 2176 2349 2458 2709 2176 Size: 173 109 251 5237 Fragments arranged by size: 5237 251 173 109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 2419 2463 2494 2620 3097 1872 Size: 547 44 31 126 477 4545 Fragments arranged by size: 4545 547 477 126 44 31 SmlI C'TyrA_G Cuts at: 820 1017 1943 2444 3874 4775 5037 820 Size: 197 926 501 1430 901 262 1553 Fragments arranged by size: 1553 1430 926 901 501 262 197 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 2485 3128 1132 Size: 1353 643 3774 Fragments arranged by size: 3774 1353 643 SrfI GCCC'GGGC Cuts at: 2620 2620 Size: 5770 SspI AAT'ATT Cuts at: 5 52 3694 5 Size: 47 3642 2081 Fragments arranged by size: 3642 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5770 StyI C'CwwG_G Cuts at: 513 1065 2004 2154 3209 513 Size: 552 939 150 1055 3074 Fragments arranged by size: 3074 1055 939 552 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2651 3797 3814 3967 4152 4597 325 Size: 1255 1071 1146 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1146 1071 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4016 5728 96 Size: 274 80 33 51 1443 2039 1712 138 Fragments arranged by size: 2039 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5730 5762 5730 Size: 32 5738 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5770 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 2417 2461 2492 2618 3095 1870 Size: 547 44 31 126 477 4545 Fragments arranged by size: 4545 547 477 126 44 31 XmnI GAAnn'nnTTC Cuts at: 3899 3899 Size: 5770 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3578 276 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII SanDI SapI SexAI SfiI SwaI XcmI