Restriction map for
pFN21AB6196
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BalI BamHI BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraII DrdI Eam1105I EcoO109I EcoO65I
EcoRV FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NdeI NheI NruI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Van91I XhoI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 685 GTCTAC GT^[AC][GT]AC
AccII 330 CGCG CG^CG
AcsI 1113 GAATTC [GA]^AATT[CT]
AocI 750 CCTGAGG CC^T[ACGT]AGG
Aor51HI 367 AGCGCT AGC^GCT
ApaI 1501 GGGCCC GGGCC^C
ApoI 1113 GAATTC [GA]^AATT[CT]
AspI 108 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 502 CCCGGG C^[CT]CG[GA]G
BbeI 763 GGCGCC GGCGC^C
BbvCI 1221 CCTCAGC CCTCAGC(-5/-2)
BfaI 851 CTAG C^TAG
BglI 586 GCCAAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 834 CTGCAC GTGCAG(16/14)
BsiEI 326 CGGCCG CG[GA][CT]^CG
BsmI 918 GCATTC GAATGC(1/-1)
BsoBI 502 CCCGGG C^[CT]CG[GA]G
BspHI 1527 TCATGA T^CATGA
BspMI 1323 ACCTGC ACCTGC(4/8)
BstUI 330 CGCG CG^CG
BstXI 1440 CCATCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 573 GGATCT [GA]^GATC[CT]
Bsu36I 750 CCTGAGG CC^T[ACGT]AGG
DraIII 661 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1196 CCATGG C^C[GA][CT]GG
EagI 326 CGGCCG C^GGCCG
EclXI 326 CGGCCG C^GGCCG
Eco47III 367 AGCGCT AGC^GCT
Eco52I 326 CGGCCG C^GGCCG
Eco81I 750 CCTGAGG CC^T[ACGT]AGG
EcoNI 630 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1113 GAATTC G^AATTC
EcoT14I 1196 CCATGG C^C[AT][AT]GG
EcoT22I 1555 ATGCAT ATGCA^T
FauI 1578 CCCGC CCCGC(4/6)
KasI 763 GGCGCC G^GCGCC
MaeI 851 CTAG C^TAG
MaeII 64 ACGT A^CGT
MflI 573 GGATCT [GA]^GATC[CT]
MvnI 330 CGCG CG^CG
NaeI 9 GCCGGC GCC^GGC
NarI 763 GGCGCC GG^CGCC
NcoI 1196 CCATGG C^CATGG
NgoMIV 9 GCCGGC G^CCGGC
NotI 325 GCGGCCGC GC^GGCCGC
NsiI 1555 ATGCAT ATGCA^T
PflFI 108 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1538 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 980 CTGCAG CTGCA^G
RcaI 1527 TCATGA T^CATGA
SapI 1418 GCTCTTC GCTCTTC(1/4)
SfcI 980 CTGCAG C^T[GA][CT]AG
SfoI 763 GGCGCC GGC^GCC
SgrAI 716 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 502 CCCGGG CCC^GGG
StyI 1196 CCATGG C^C[AT][AT]GG
TfiI 1430 GAATC G^A[AT]TC
Tth111I 108 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 850 TCTAGA T^CTAGA
XcmI 1229 CCATTGCAAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 573 GGATCT [GA]^GATC[CT]
XmaCI 502 CCCGGG C^CCGGG
XmaI 502 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 21 GATCC GGATC(4/5)
573 GGATC
Asp700I 736 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
807 GAAGGACTTC
AspHI 1416 GAGCTC G[AT]GC[AT]^C
1754 GAGCTC
BanI 82 GGCACC G^G[CT][GA]CC
763 GGCGCC
BbsI 2087 GAAGAC GAAGAC(2/6)
1689 GTCTTC
BpuAI 2087 GAAGAC GAAGAC(2/6)
1689 GTCTTC
BseRI 75 CTCCTC GAGGAG(10/8)
1339 GAGGAG
BsiHKAI 1416 GAGCTC G[AT]GC[AT]^C
1754 GAGCTC
BsmBI 113 CGTCTC CGTCTC(1/5)
1939 GAGACG
CfoI 368 GCGC GCG^C
764 GCGC
HaeII 367 AGCGCT [GA]GCGC^[CT]
763 GGCGCC
HhaI 368 GCGC GCG^C
764 GCGC
HinP1I 368 GCGC G^CGC
764 GCGC
SacI 1416 GAGCTC GAGCT^C
1754 GAGCTC
StuI 862 AGGCCT AGG^CCT
1351 AGGCCT
XmnI 736 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
807 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AvaII BanII BbvI BcnI BmyI
BpmI BsaHI BsaI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI Cac8I Cfr10I Cfr13I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I