(Circular) (Six-base) MAPSORT of: pFN21AB6196.seq Check: 712 from: 1 to: 6823 pFN21AB6196 6823 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3337 3337 Size: 6823 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4633 278 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2691 4166 1937 Size: 754 1475 4594 Fragments arranged by size: 4594 1475 754 Acc65I G'GTAC_C Cuts at: 4144 4144 Size: 6823 AclI AA'CG_TT Cuts at: 1563 4950 5323 1563 Size: 3387 373 3063 Fragments arranged by size: 3387 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2681 2723 3042 3818 4886 6270 6538 1632 Size: 1049 42 319 776 1068 1384 268 1917 Fragments arranged by size: 1917 1384 1068 1049 776 319 268 42 AfeI AGC'GCT Cuts at: 2374 2374 Size: 6823 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6626 AflIII A'CryG_T Cuts at: 1203 5722 6699 1203 Size: 4519 977 1327 Fragments arranged by size: 4519 1327 977 AgeI A'CCGG_T Cuts at: 5654 5654 Size: 6823 AhdI GACnn_n'nnGTC Cuts at: 5552 5552 Size: 6823 AleI CACnn'nnGTG Cuts at: 1166 2723 1166 Size: 1557 5266 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6823 AlwNI CAG_nnn'CTG Cuts at: 2313 2493 2984 2993 2996 3101 3194 3475 3730 Size: 180 491 9 3 105 93 281 255 Cuts at: 3730 4070 4566 6138 2313 Size: 340 496 1572 2998 Fragments arranged by size: 2998 1572 496 491 340 281 255 180 105 93 9 3 ApaI G_GGCC'C Cuts at: 3510 3510 Size: 6823 ApaLI G'TGCA_C Cuts at: 4880 6036 4880 Size: 1156 5667 ApoI r'AATT_y Cuts at: 1493 3118 4132 4358 4510 6619 1493 Size: 1625 1014 226 152 2109 1697 Fragments arranged by size: 2109 1697 1625 1014 226 152 AseI AT'TA_AT Cuts at: 160 1032 5377 160 Size: 872 4345 1606 Fragments arranged by size: 4345 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 AvaI C'yCGr_G Cuts at: 1870 1943 2507 4148 1870 Size: 73 564 1641 4545 Fragments arranged by size: 4545 1641 564 73 BamHI G'GATC_C Cuts at: 4153 4520 4153 Size: 367 6456 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 2768 4144 5599 618 Size: 325 217 627 300 681 1376 1455 1842 Fragments arranged by size: 1842 1455 1376 681 627 325 300 217 BanII G_rGCy'C Cuts at: 1391 2181 3425 3510 3763 4142 1391 Size: 790 1244 85 253 379 4072 Fragments arranged by size: 4072 1244 790 379 253 85 BbeI G_GCGC'C Cuts at: 2772 2772 Size: 6823 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3687 4099 928 Size: 971 1788 412 3652 Fragments arranged by size: 3652 1788 971 412 BbvCI CC'TCA_GC Cuts at: 3227 3227 Size: 6823 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5014 5048 1903 Size: 34 3077 34 3678 Fragments arranged by size: 3678 3077 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4715 5925 4715 Size: 1210 5613 BclI T'GATC_A Cuts at: 1548 1548 Size: 6823 BfrBI ATG'CAT Cuts at: 3562 3562 Size: 6823 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2597 5434 136 Size: 107 122 71 631 605 925 2837 1525 Fragments arranged by size: 2837 1525 925 631 605 122 107 71 BglII A'GATC_T Cuts at: 6818 6818 Size: 6823 BlpI GC'TnA_GC Cuts at: 4240 4240 Size: 6823 Bme1580I G_kGCm'C Cuts at: 1790 3510 4884 6040 1790 Size: 1720 1374 1156 2573 Fragments arranged by size: 2573 1720 1374 1156 BmgBI CAC'GTC Cuts at: 1318 2070 1318 Size: 752 6071 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2531 2774 3642 Size: 416 53 83 399 24 1114 243 868 Cuts at: 3642 4554 5512 442 Size: 912 958 1753 Fragments arranged by size: 1753 1114 958 912 868 416 399 243 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6823 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2732 2791 3171 3362 3639 5483 1239 Size: 1493 59 380 191 277 1844 2579 Fragments arranged by size: 2579 1844 1493 380 277 191 59 Bpu10I CC'TnA_GC Cuts at: 3227 3227 Size: 6823 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3747 3860 3956 4948 5813 6111 3747 Size: 113 96 992 865 298 4459 Fragments arranged by size: 4459 992 865 298 113 96 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2816 2942 3572 5486 882 Size: 617 1317 126 630 1914 2219 Fragments arranged by size: 2219 1914 1317 630 617 126 BsaAI yAC'GTr Cuts at: 493 1204 4614 493 Size: 711 3410 2702 Fragments arranged by size: 3410 2702 711 BsaBI GATnn'nnATC Cuts at: 6817 6817 Size: 6823 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2147 2385 2769 4630 Size: 53 83 186 936 614 238 384 1861 Cuts at: 4630 5012 5647 275 Size: 382 635 1451 Fragments arranged by size: 1861 1451 936 635 614 384 382 238 186 83 53 BsaWI w'CCGG_w Cuts at: 5256 5654 5928 6075 6486 5256 Size: 398 274 147 411 5593 Fragments arranged by size: 5593 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2104 2134 1178 Size: 30 165 30 701 30 5867 Fragments arranged by size: 5867 701 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2071 3359 1186 Size: 885 1288 4650 Fragments arranged by size: 4650 1288 885 BseYI C'CCAG_C Cuts at: 2037 6026 6522 2037 Size: 3989 496 2338 Fragments arranged by size: 3989 2338 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2824 1163 Size: 573 1088 5162 Fragments arranged by size: 5162 1088 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2334 4273 5034 6062 1540 Size: 275 186 333 1939 761 1028 2301 Fragments arranged by size: 2301 1939 1028 761 333 275 186 BsiHKAI G_wGCw'C Cuts at: 3425 3763 4884 4969 6040 3425 Size: 338 1121 85 1071 4208 Fragments arranged by size: 4208 1121 1071 338 85 BsmI GAATG_Cn' Cuts at: 2923 4334 4427 2923 Size: 1411 93 5319 Fragments arranged by size: 5319 1411 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2124 3938 2124 Size: 1814 5009 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 3425 3510 3763 4142 4884 4969 Size: 399 391 1244 85 253 379 742 85 Cuts at: 4969 6040 1391 Size: 1071 2174 Fragments arranged by size: 2174 1244 1071 742 399 391 379 253 85 85 BspEI T'CCGG_A Cuts at: 6486 6486 Size: 6823 BspHI T'CATG_A Cuts at: 3532 4712 6442 3532 Size: 1180 1730 3913 Fragments arranged by size: 3913 1730 1180 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3337 4178 844 Size: 2493 841 3489 Fragments arranged by size: 3489 2493 841 BsrBI CCG'CTC Cuts at: 1394 1823 4710 1394 Size: 429 2887 3507 Fragments arranged by size: 3507 2887 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2740 3033 3213 3234 5318 5492 65 Size: 1183 1492 293 180 21 2084 174 1396 Fragments arranged by size: 2084 1492 1396 1183 293 180 174 21 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2222 2417 2722 3527 5467 5654 Size: 110 204 208 195 305 805 1940 187 Cuts at: 5654 1700 Size: 2869 Fragments arranged by size: 2869 1940 805 305 208 204 195 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6823 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6823 BssSI C'ACGA_G Cuts at: 4883 5895 4883 Size: 1012 5811 BstAPI GCAn_nnn'nTGC Cuts at: 794 2313 2493 2984 2993 2996 3017 794 Size: 1519 180 491 9 3 21 4600 Fragments arranged by size: 4600 1519 491 180 21 9 3 BstBI TT'CG_AA Cuts at: 5627 5627 Size: 6823 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6823 BstXI CCAn_nnnn'nTGG Cuts at: 3452 3452 Size: 6823 BstYI r'GATC_y Cuts at: 1972 2578 4153 4520 4905 4922 6374 6460 6472 Size: 606 1575 367 385 17 1452 86 12 Cuts at: 6472 6818 1972 Size: 346 1977 Fragments arranged by size: 1977 1575 1452 606 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2756 2756 Size: 6823 BtgI C'CryG_G Cuts at: 513 1065 2004 3201 513 Size: 552 939 1197 4135 Fragments arranged by size: 4135 1197 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2167 3365 487 Size: 683 56 941 1198 3945 Fragments arranged by size: 3945 1198 941 683 56 BtsI GCAGTG_nn' Cuts at: 2016 3647 4057 4339 5133 5153 2016 Size: 1631 410 282 794 20 3686 Fragments arranged by size: 3686 1631 794 410 282 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6823 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6788 DraI TTT'AAA Cuts at: 4127 4127 Size: 6823 DraIII CAC_nnn'GTG Cuts at: 2671 2671 Size: 6823 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5830 809 Size: 507 4514 1802 Fragments arranged by size: 4514 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2205 2331 3525 Size: 54 1425 50 275 18 375 126 1194 Cuts at: 3525 4270 5159 6788 8 Size: 745 889 1629 43 Fragments arranged by size: 1629 1425 1194 889 745 375 275 126 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2331 4270 1537 Size: 275 519 1939 4090 Fragments arranged by size: 4090 1939 519 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2110 3430 3714 4753 1353 Size: 757 1320 284 1039 3423 Fragments arranged by size: 3423 1320 1039 757 284 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2159 5410 5784 5930 387 Size: 1064 138 570 3251 374 146 1280 Fragments arranged by size: 3251 1280 1064 570 374 146 138 EcoICRI GAG'CTC Cuts at: 3423 3761 3423 Size: 338 6485 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2681 2723 2732 2791 3042 3171 3362 Size: 393 1049 42 9 59 251 129 191 Cuts at: 3362 3639 3818 4886 5483 6270 6538 1239 Size: 277 179 1068 597 787 268 1524 Fragments arranged by size: 1524 1068 1049 787 597 393 277 268 251 191 179 129 59 42 9 EcoNI CCTnn'n_nnAGG Cuts at: 2639 2639 Size: 6823 EcoRI G'AATT_C Cuts at: 3118 4132 4510 3118 Size: 1014 378 5431 Fragments arranged by size: 5431 1014 378 FspI TGC'GCA Cuts at: 5329 5329 Size: 6823 HaeII r_GCGC'y Cuts at: 1678 2376 2772 5970 1678 Size: 698 396 3198 2531 Fragments arranged by size: 3198 2531 698 396 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2104 2136 3052 3084 3308 Size: 32 135 32 532 32 916 32 224 Cuts at: 3308 3340 3608 3640 3677 3709 5464 5496 5538 Size: 32 268 32 37 32 1755 32 42 Cuts at: 5538 5570 1373 Size: 32 2626 Fragments arranged by size: 2626 1755 916 532 268 224 135 42 37 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4167 4413 669 Size: 988 281 2229 246 3079 Fragments arranged by size: 3079 2229 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6823 HpaI GTT'AAC Cuts at: 4413 4413 Size: 6823 KasI G'GCGC_C Cuts at: 2768 2768 Size: 6823 KpnI G_GTAC'C Cuts at: 4148 4148 Size: 6823 MfeI C'AATT_G Cuts at: 4422 4422 Size: 6823 MluI A'CGCG_T Cuts at: 6699 6699 Size: 6823 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2057 3109 3802 5937 6121 1305 Size: 752 1052 693 2135 184 2007 Fragments arranged by size: 2135 2007 1052 752 693 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5001 Fragments arranged by size: 5001 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2723 3206 3450 4781 5140 5299 Size: 648 843 714 483 244 1331 359 159 Cuts at: 5299 518 Size: 2042 Fragments arranged by size: 2042 1331 843 714 648 483 359 244 159 MspA1I CmG'CkG Cuts at: 1606 2041 2250 2271 2349 2493 2793 2984 2993 Size: 435 209 21 78 144 300 191 9 Cuts at: 2993 4239 4916 6064 6309 1606 Size: 1246 677 1148 245 2120 Fragments arranged by size: 2120 1246 1148 677 435 300 245 209 191 144 78 21 9 NaeI GCC'GGC Cuts at: 2016 2016 Size: 6823 NarI GG'CG_CC Cuts at: 2769 2769 Size: 6823 NcoI C'CATG_G Cuts at: 513 1065 2004 3201 513 Size: 552 939 1197 4135 Fragments arranged by size: 4135 1197 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6823 NgoMIV G'CCGG_C Cuts at: 2014 2014 Size: 6823 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6823 NotI GC'GGCC_GC Cuts at: 2331 4270 2331 Size: 1939 4884 NsiI A_TGCA'T Cuts at: 3564 3564 Size: 6823 NspI r_CATG'y Cuts at: 1132 1229 4181 5726 1132 Size: 97 2952 1545 2229 Fragments arranged by size: 2952 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6823 PciI A'CATG_T Cuts at: 5722 5722 Size: 6823 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6823 PfoI T'CCnGG_A Cuts at: 2807 2807 Size: 6823 PmeI GTTT'AAAC Cuts at: 4127 4127 Size: 6823 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3864 3896 4892 4924 6405 6437 3864 Size: 32 996 32 1481 32 4250 Fragments arranged by size: 4250 1481 996 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3547 1613 Size: 1934 4889 PsiI TTA'TAA Cuts at: 4393 4393 Size: 6823 PspOMI G'GGCC_C Cuts at: 3506 3506 Size: 6823 PstI C_TGCA'G Cuts at: 830 2989 4175 830 Size: 2159 1186 3478 Fragments arranged by size: 3478 2159 1186 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6823 PvuII CAG'CTG Cuts at: 2041 2493 2993 2041 Size: 452 500 5871 Fragments arranged by size: 5871 500 452 SacI G_AGCT'C Cuts at: 3425 3763 3425 Size: 338 6485 SalI G'TCGA_C Cuts at: 1936 4165 1936 Size: 2229 4594 SapI GCTCTTCn'nnn_ Cuts at: 3430 3430 Size: 6823 SbfI CC_TGCA'GG Cuts at: 4175 4175 Size: 6823 ScaI AGT'ACT Cuts at: 5071 6783 5071 Size: 1712 5111 SfcI C'TryA_G Cuts at: 826 1045 2985 4171 5306 5987 6178 826 Size: 219 1940 1186 1135 681 191 1471 Fragments arranged by size: 1940 1471 1186 1135 681 219 191 SfoI GGC'GCC Cuts at: 2770 2770 Size: 6823 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6823 SgrAI Cr'CCGG_yG Cuts at: 1700 2722 1700 Size: 1022 5801 SmaI CCC'GGG Cuts at: 1872 2509 4150 1872 Size: 637 1641 4545 Fragments arranged by size: 4545 1641 637 SmlI C'TyrA_G Cuts at: 820 1017 1943 3762 3839 3935 4927 5828 6090 Size: 197 926 1819 77 96 992 901 262 Cuts at: 6090 820 Size: 1553 Fragments arranged by size: 1819 1553 992 926 901 262 197 96 77 SnaBI TAC'GTA Cuts at: 493 4614 493 Size: 4121 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6823 SphI G_CATG'C Cuts at: 1132 4181 1132 Size: 3049 3774 SspI AAT'ATT Cuts at: 5 52 4747 5 Size: 47 4695 2081 Fragments arranged by size: 4695 2081 47 StuI AGG'CCT Cuts at: 1517 2869 3358 1517 Size: 1352 489 4982 Fragments arranged by size: 4982 1352 489 StyI C'CwwG_G Cuts at: 513 1065 2004 3201 4262 513 Size: 552 939 1197 1061 3074 Fragments arranged by size: 3074 1197 1061 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3384 4850 4867 5020 5205 5650 325 Size: 1255 1804 1466 17 153 185 445 1498 Fragments arranged by size: 1804 1498 1466 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2520 3496 5069 Size: 274 80 33 51 1443 543 976 1573 Cuts at: 5069 6781 96 Size: 1712 138 Fragments arranged by size: 1712 1573 1443 976 543 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2872 2904 6783 6815 2872 Size: 32 3879 32 2880 Fragments arranged by size: 3879 2880 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2116 1316 Size: 800 6023 XbaI T'CTAG_A Cuts at: 2855 4159 2855 Size: 1304 5519 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3241 3241 Size: 6823 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6823 XmaI C'CCGG_G Cuts at: 1870 2507 4148 1870 Size: 637 1641 4545 Fragments arranged by size: 4545 1641 637 XmnI GAAnn'nnTTC Cuts at: 2745 2816 4952 2745 Size: 71 2136 4616 Fragments arranged by size: 4616 2136 71 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4631 276 Size: 53 83 186 936 3097 2468 Fragments arranged by size: 3097 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BplI BsiWI BstZ17I EcoO109I EcoRV FalI FseI FspAI NruI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI