Restriction map for
pFN21AB6196
(Linear) (Six-base) MAPSORT of: KIBB6196.seq Check: 7040 from: 1 to: 2118
KIBB6196.orf
With 182 enzymes: SgfI *
July 3, 2012 16:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1332 2118
Size: 1332 786
AccI GT'mk_AC
Cuts at: 0 686 2118
Size: 686 1432
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 718 1037 1813 2118
Size: 676 42 319 776 305
Fragments arranged by size:
776 676 319 305 42
AfeI AGC'GCT
Cuts at: 0 369 2118
Size: 369 1749
AleI CACnn'nnGTG
Cuts at: 0 718 2118
Size: 718 1400
AlwNI CAG_nnn'CTG
Cuts at: 0 308 488 979 988 991 1096 1189 1470
Size: 308 180 491 9 3 105 93 281
Cuts at: 1470 1725 2065 2118
Size: 255 340 53
Fragments arranged by size:
491 340 308 281 255 180 105 93
53 9 3
ApaI G_GGCC'C
Cuts at: 0 1505 2118
Size: 1505 613
ApoI r'AATT_y
Cuts at: 0 1113 2118
Size: 1113 1005
AvaI C'yCGr_G
Cuts at: 0 502 2118
Size: 502 1616
BanI G'GyrC_C
Cuts at: 0 82 763 2118
Size: 82 681 1355
Fragments arranged by size:
1355 681 82
BanII G_rGCy'C
Cuts at: 0 176 1420 1505 1758 2118
Size: 176 1244 85 253 360
Fragments arranged by size:
1244 360 253 176 85
BbeI G_GCGC'C
Cuts at: 0 767 2118
Size: 767 1351
BbsI GAAGACnn'nnnn_
Cuts at: 0 1682 2094 2118
Size: 1682 412 24
Fragments arranged by size:
1682 412 24
BbvCI CC'TCA_GC
Cuts at: 0 1222 2118
Size: 1222 896
BfrBI ATG'CAT
Cuts at: 0 1557 2118
Size: 1557 561
BglI GCCn_nnn'nGGC
Cuts at: 0 592 2118
Size: 592 1526
Bme1580I G_kGCm'C
Cuts at: 0 1505 2118
Size: 1505 613
BmgBI CAC'GTC
Cuts at: 0 65 2118
Size: 65 2053
BmrI ACTGGGnnnn_n'
Cuts at: 0 526 769 1637 2118
Size: 526 243 868 481
Fragments arranged by size:
868 526 481 243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 786 1166 1357 1634 2118
Size: 727 59 380 191 277 484
Fragments arranged by size:
727 484 380 277 191 59
Bpu10I CC'TnA_GC
Cuts at: 0 1222 2118
Size: 1222 896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1742 1855 1951 2118
Size: 1742 113 96 167
Fragments arranged by size:
1742 167 113 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 811 937 1567 2118
Size: 811 126 630 551
Fragments arranged by size:
811 630 551 126
BsaHI Gr'CG_yC
Cuts at: 0 142 380 764 2118
Size: 142 238 384 1354
Fragments arranged by size:
1354 384 238 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 99 129 2118
Size: 99 30 1989
Fragments arranged by size:
1989 99 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 66 1354 2118
Size: 66 1288 764
Fragments arranged by size:
1288 764 66
BseYI C'CCAG_C
Cuts at: 0 32 2118
Size: 32 2086
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 819 2118
Size: 819 1299
BsiEI CG_ry'CG
Cuts at: 0 329 2118
Size: 329 1789
BsiHKAI G_wGCw'C
Cuts at: 0 1420 1758 2118
Size: 1420 338 360
Fragments arranged by size:
1420 360 338
BsmI GAATG_Cn'
Cuts at: 0 918 2118
Size: 918 1200
BsmBI CGTCTCn'nnnn_
Cuts at: 0 119 1933 2118
Size: 119 1814 185
Fragments arranged by size:
1814 185 119
Bsp1286I G_dGCh'C
Cuts at: 0 176 1420 1505 1758 2118
Size: 176 1244 85 253 360
Fragments arranged by size:
1244 360 253 176 85
BspHI T'CATG_A
Cuts at: 0 1527 2118
Size: 1527 591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1332 2118
Size: 1332 786
BsrDI GCAATG_nn'
Cuts at: 0 735 1028 1208 1229 2118
Size: 735 293 180 21 889
Fragments arranged by size:
889 735 293 180 21
BsrFI r'CCGG_y
Cuts at: 0 9 217 412 717 1522 2118
Size: 9 208 195 305 805 596
Fragments arranged by size:
805 596 305 208 195 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 308 488 979 988 991 1012 2118
Size: 308 180 491 9 3 21 1106
Fragments arranged by size:
1106 491 308 180 21 9 3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1447 2118
Size: 1447 671
BstYI r'GATC_y
Cuts at: 0 573 2118
Size: 573 1545
Bsu36I CC'TnA_GG
Cuts at: 0 751 2118
Size: 751 1367
BtgI C'CryG_G
Cuts at: 0 1196 2118
Size: 1196 922
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 162 1360 2118
Size: 162 1198 758
Fragments arranged by size:
1198 758 162
BtsI GCAGTG_nn'
Cuts at: 0 11 1642 2052 2118
Size: 11 1631 410 66
Fragments arranged by size:
1631 410 66 11
DraIII CAC_nnn'GTG
Cuts at: 0 666 2118
Size: 666 1452
EaeI y'GGCC_r
Cuts at: 0 200 326 1520 2118
Size: 200 126 1194 598
Fragments arranged by size:
1194 598 200 126
EagI C'GGCC_G
Cuts at: 0 326 2118
Size: 326 1792
EarI CTCTTCn'nnn_
Cuts at: 0 105 1425 1709 2118
Size: 105 1320 284 409
Fragments arranged by size:
1320 409 284 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 154 2118
Size: 154 1964
EcoICRI GAG'CTC
Cuts at: 0 1418 1756 2118
Size: 1418 338 362
Fragments arranged by size:
1418 362 338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 718 727 786 1037 1166 1357 1634
Size: 676 42 9 59 251 129 191 277
Cuts at: 1634 1813 2118
Size: 179 305
Fragments arranged by size:
676 305 277 251 191 179 129 59
42 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 634 2118
Size: 634 1484
EcoRI G'AATT_C
Cuts at: 0 1113 2118
Size: 1113 1005
HaeII r_GCGC'y
Cuts at: 0 371 767 2118
Size: 371 396 1351
Fragments arranged by size:
1351 396 371
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 99 131 1047 1079 1303 1335 1603 1635
Size: 99 32 916 32 224 32 268 32
Cuts at: 1635 1672 1704 2118
Size: 37 32 414
Fragments arranged by size:
916 414 268 224 99 37 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 763 2118
Size: 763 1355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 1104 1797 2118
Size: 52 1052 693 321
Fragments arranged by size:
1052 693 321 52
MslI CAynn'nnrTG
Cuts at: 0 718 1201 1445 2118
Size: 718 483 244 673
Fragments arranged by size:
718 673 483 244
MspA1I CmG'CkG
Cuts at: 0 36 245 266 344 488 788 979 988
Size: 36 209 21 78 144 300 191 9
Cuts at: 988 2118
Size: 1130
Fragments arranged by size:
1130 300 209 191 144 78 36 21
9
NaeI GCC'GGC
Cuts at: 0 11 2118
Size: 11 2107
NarI GG'CG_CC
Cuts at: 0 764 2118
Size: 764 1354
NcoI C'CATG_G
Cuts at: 0 1196 2118
Size: 1196 922
NgoMIV G'CCGG_C
Cuts at: 0 9 2118
Size: 9 2109
NotI GC'GGCC_GC
Cuts at: 0 326 2118
Size: 326 1792
NsiI A_TGCA'T
Cuts at: 0 1559 2118
Size: 1559 559
PfoI T'CCnGG_A
Cuts at: 0 802 2118
Size: 802 1316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1859 1891 2118
Size: 1859 32 227
Fragments arranged by size:
1859 227 32
PshAI GACnn'nnGTC
Cuts at: 0 1542 2118
Size: 1542 576
PspOMI G'GGCC_C
Cuts at: 0 1501 2118
Size: 1501 617
PstI C_TGCA'G
Cuts at: 0 984 2118
Size: 984 1134
PvuII CAG'CTG
Cuts at: 0 36 488 988 2118
Size: 36 452 500 1130
Fragments arranged by size:
1130 500 452 36
SacI G_AGCT'C
Cuts at: 0 1420 1758 2118
Size: 1420 338 360
Fragments arranged by size:
1420 360 338
SapI GCTCTTCn'nnn_
Cuts at: 0 1425 2118
Size: 1425 693
SfcI C'TryA_G
Cuts at: 0 980 2118
Size: 980 1138
SfoI GGC'GCC
Cuts at: 0 765 2118
Size: 765 1353
SgrAI Cr'CCGG_yG
Cuts at: 0 717 2118
Size: 717 1401
SmaI CCC'GGG
Cuts at: 0 504 2118
Size: 504 1614
SmlI C'TyrA_G
Cuts at: 0 1757 1834 1930 2118
Size: 1757 77 96 188
Fragments arranged by size:
1757 188 96 77
StuI AGG'CCT
Cuts at: 0 864 1353 2118
Size: 864 489 765
Fragments arranged by size:
864 765 489
StyI C'CwwG_G
Cuts at: 0 1196 2118
Size: 1196 922
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1379 2118
Size: 1379 739
TatI w'GTAC_w
Cuts at: 0 515 1491 2118
Size: 515 976 627
Fragments arranged by size:
976 627 515
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 867 899 2118
Size: 867 32 1219
Fragments arranged by size:
1219 867 32
Tth111I GACn'n_nGTC
Cuts at: 0 111 2118
Size: 111 2007
XbaI T'CTAG_A
Cuts at: 0 850 2118
Size: 850 1268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1236 2118
Size: 1236 882
XmaI C'CCGG_G
Cuts at: 0 502 2118
Size: 502 1616
XmnI GAAnn'nnTTC
Cuts at: 0 740 811 2118
Size: 740 71 1307
Fragments arranged by size:
1307 740 71
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BfrBI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI HaeII Hin4I KasI
MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NsiI PfoI PpiI PshAI PspOMI PstI PvuII SacI
SapI SfcI SfoI SgrAI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BglII BlpI BmtI BplI BsaAI
BsaBI BsaWI BsiWI BspEI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EcoO109I
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NdeI NheI NruI NspI
PacI PasI PciI PflMI PmeI PmlI PpuMI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI
SspI SwaI XhoI ZraI