################################## # NO CUT ENZYMES ( 200 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AciI 69 CCGC CCGC(-3/-1) AgeI 107 ACCGGT A^CCGGT AhdI 314 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 211 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 314 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 110 GGTGCC G^G[CT][GA]CC BbsI 215 GTCTTC GAAGAC(2/6) BbvI 253 GCTGC GCAGC(8/12) BpuAI 215 GTCTTC GAAGAC(2/6) BsaJI 17 CCAAGG C^C[ACGT][ACGT]GG BsaWI 107 ACCGGT [AT]^CCGG[AT] BsiYI 267 CCTGAATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 267 CCTGAATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrFI 107 ACCGGT [GA]^CCGG[CT] BstXI 339 CCATGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 107 ACCGGT [GA]^CCGG[CT] Cfr13I 264 GGGCC G^G[ACGT]CC DdeI 23 CTCAG C^T[ACGT]AG Eam1105I 314 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 267 CCTGAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 17 CCAAGG C^C[AT][AT]GG EcoT22I 361 ATGCAT ATGCA^T Fnu4HI 253 GCTGC GC^[ACGT]GC HapII 108 CCGG C^CGG HpaII 108 CCGG C^CGG ItaI 253 GCTGC GC^[ACGT]GC MaeII 329 ACGT A^CGT MseI 229 TTAA T^TAA MslI 334 CATCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 108 CCGG C^CGG MwoI 22 GCTCAGGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 110 GGTGCC GG[ACGT]^[ACGT]CC NsiI 361 ATGCAT ATGCA^T PinAI 107 ACCGGT A^CCGGT Sau96I 264 GGGCC G^G[ACGT]CC SmlI 137 CTCAAG C^T[CT][GA]AG StyI 17 CCAAGG C^C[AT][AT]GG TaqI 282 TCGA T^CGA Tru9I 229 TTAA T^TAA TseI 253 GCTGC G^C[AT]GC TthHB8I 282 TCGA T^CGA XmnI 211 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### BssKI 37 CCTGG ^CC[ACGT]GG 373 CCTGG BstF5I 66 CATCC GGATG(2/0) 102 GGATG BstNI 37 CCTGG CC^[AT]GG 373 CCTGG EcoRII 37 CCTGG ^CC[AT]GG 373 CCTGG FokI 66 CATCC GGATG(9/13) 102 GGATG HphI 159 GGTGA GGTGA(8/7) 291 GGTGA MvaI 37 CCTGG CC^[AT]GG 373 CCTGG NlaIII 124 CATG CATG^ 340 CATG ScrFI 37 CCTGG CC^[ACGT]GG 373 CCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### DpnII HaeIII MboI MboII MnlI NdeII Sau3AI