################################## # NO CUT ENZYMES ( 216 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AflII 94 CTTAAG C^TTAAG AlwI 79 GATCC GGATC(4/5) BanI 151 GGCACC G^G[CT][GA]CC BbvI 179 GCTGC GCAGC(8/12) BfaI 131 CTAG C^TAG BfrI 94 CTTAAG C^TTAAG BsaI 160 GGTCTC GGTCTC(1/5) BsaJI 165 CCCTGG C^C[ACGT][ACGT]GG BseRI 16 CTCCTC GAGGAG(10/8) BsiYI 49 CCTCTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 49 CCTCTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 161 GTCTC GTCTC(1/5) BspMI 88 ACCTGC ACCTGC(4/8) BsrI 118 CCAGT ACTGG(1/-1) BstF5I 115 CATCC GGATG(2/0) DpnII 79 GATC ^GATC EcoNI 49 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Fnu4HI 179 GCTGC GC^[ACGT]GC FokI 115 CATCC GGATG(9/13) ItaI 179 GCTGC GC^[ACGT]GC MaeI 131 CTAG C^TAG MaeIII 85 GTTAC ^GT[ACGT]AC MboI 79 GATC ^GATC MseI 95 TTAA T^TAA NdeI 108 CATATG CA^TATG NdeII 79 GATC ^GATC Sau3AI 79 GATC ^GATC SfaNI 45 GATGC GCATC(5/9) SmlI 94 CTTAAG C^T[CT][GA]AG Tru9I 95 TTAA T^TAA TseI 179 GCTGC G^C[AT]GC Tsp509I 147 AATT ^AATT ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### BssKI 55 CCAGG ^CC[ACGT]GG 166 CCTGG BstNI 55 CCAGG CC^[AT]GG 166 CCTGG EcoRII 55 CCAGG ^CC[AT]GG 166 CCTGG MvaI 55 CCAGG CC^[AT]GG 166 CCTGG NlaIV 103 GGAGCC GG[ACGT]^[ACGT]CC 151 GGCACC ScrFI 55 CCAGG CC^[ACGT]GG 166 CCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### EarI Ksp632I MboII MnlI