Restriction map for
pFN21AB7334
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
AhdI Alw44I ApaI ApaLI AscI
AspEI AspI AviII BamHI BbvCI
BcgI BfrI BlpI Bpu1102I BsaI
BseAI BsgI BsiEI BsiWI BspDI
BspEI BspLU11I BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco52I EcoO65I
FseI FspI HindIII HpaI KspI
MluI MroI MvnI NcoI NdeI
NotI NruI NspI PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 169 GACGTC GACGT^C
Acc65I 491 GGTACC G^GTACC
AflIII 2548 ACACGT A^C[GA][CT]GT
AocI 1867 CCTGAGG CC^T[ACGT]AGG
Aor51HI 92 AGCGCT AGC^GCT
AseI 421 ATTAAT AT^TAAT
AsnI 421 ATTAAT AT^TAAT
Asp718I 491 GGTACC G^GTACC
AvrII 3979 CCTAGG C^CTAGG
BalI 1971 TGGCCA TGG^CCA
BbeI 156 GGCGCC GGCGC^C
BbrPI 2549 CACGTG CAC^GTG
BciVI 2743 GGATAC GTATCC(6/5)
BglI 3462 GCCAATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3979 CCTAGG C^CTAGG
BsaAI 2549 CACGTG [CT]AC^GT[GA]
Bsp1407I 524 TGTACA T^GTACA
BspHI 1305 TCATGA T^CATGA
BspMI 113 ACCTGC ACCTGC(4/8)
BsrBI 142 GAGCGG CCGCTC(-3/-3)
BsrDI 2573 GCAATG GCAATG(2/0)
BsrFI 3263 GCCGGC [GA]^CCGG[CT]
BsrGI 524 TGTACA T^GTACA
BstAPI 3238 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3812 TTCGAA TT^CGAA
Bsu36I 1867 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3263 GCCGGC [GA]^CCGG[CT]
DrdI 1186 GACAGTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 92 AGCGCT AGC^GCT
Eco81I 1867 CCTGAGG CC^T[ACGT]AGG
EcoRI 3322 GAATTC G^AATTC
EcoT22I 567 ATGCAT ATGCA^T
KasI 156 GGCGCC G^GCGCC
KpnI 491 GGTACC GGTAC^C
MluNI 1971 TGGCCA TGG^CCA
MscI 1971 TGGCCA TGG^CCA
NaeI 3263 GCCGGC GCC^GGC
NarI 156 GGCGCC GG^CGCC
NgoMIV 3263 GCCGGC G^CCGGC
NheI 3786 GCTAGC G^CTAGC
NsiI 567 ATGCAT ATGCA^T
NspV 3812 TTCGAA TT^CGAA
PmaCI 2549 CACGTG CAC^GTG
PmlI 2549 CACGTG CAC^GTG
PshBI 421 ATTAAT AT^TAAT
RcaI 1305 TCATGA T^CATGA
SacI 205 GAGCTC GAGCT^C
SapI 554 GCTCTTC GCTCTTC(1/4)
SfoI 156 GGCGCC GGC^GCC
SfuI 3812 TTCGAA TT^CGAA
SgrAI 3262 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 48 CCCGGG CCC^GGG
SspBI 524 TGTACA T^GTACA
XmaCI 48 CCCGGG C^CCGGG
XmaI 48 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1990 GTAGAC GT^[AC][GT]AC
3437 GTAGAC
AcyI 156 GGCGCC G[GA]^CG[CT]C
169 GACGTC
AlwNI 297 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
3239 CAGCTGCTG
Asp700I 1822 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3725 GAAGACTTTC
AvaI 48 CCCGGG C^[CT]CG[GA]G
671 CTCGGG
AvaII 1201 GGACC G^G[AT]CC
3984 GGTCC
BanI 156 GGCGCC G^G[CT][GA]CC
491 GGTACC
BglII 1644 AGATCT A^GATCT
2329 AGATCT
BsaHI 156 GGCGCC G[GA]^CG[CT]C
169 GACGTC
BsaWI 3902 ACCGGA [AT]^CCGG[AT]
4022 ACCGGA
BsmBI 448 CGTCTC CGTCTC(1/5)
3910 GAGACG
BsmI 349 GAATGC GAATGC(1/-1)
1945 GAATGC
BsoBI 48 CCCGGG C^[CT]CG[GA]G
671 CTCGGG
BstXI 453 CCAGCCAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2058 CCAGACCTCTGG
DraII 1200 GGGACCT [GA]G^G[ACGT]CC[CT]
3983 GGGTCCT
EcoNI 2004 CCTAAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2174 CCTCTGAAAGG
EcoO109I 1200 GGGACCT [GA]G^G[ACGT]CC[CT]
3983 GGGTCCT
EcoRV 2214 GATATC GAT^ATC
3151 GATATC
EcoT14I 3979 CCTAGG C^C[AT][AT]GG
4181 CCAAGG
HgaI 1795 GACGC GACGC(5/10)
447 GCGTC
Hin1I 156 GGCGCC G[GA]^CG[CT]C
169 GACGTC
HincII 2430 GTCAAC GT[CT]^[GA]AC
3033 GTCAAC
HindII 2430 GTCAAC GT[CT]^[GA]AC
3033 GTCAAC
MslI 560 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3943 CATGGAGATG
PpuMI 1200 GGGACCT [GA]G^G[AT]CC[CT]
3983 GGGTCCT
ScaI 1837 AGTACT AGT^ACT
1960 AGTACT
SmlI 1504 CTTGAG C^T[CT][GA]AG
3344 CTCAAG
StyI 3979 CCTAGG C^C[AT][AT]GG
4181 CCAAGG
XmnI 1822 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3725 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaBI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MfeI MflI
MnlI MseI MspA1I MspI MunI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII