Restriction map for
pFN21AB7334
(Linear) (Six-base) MAPSORT of: KIBB7334.seq Check: 7049 from: 1 to: 4323
KIBB7334.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AatII G_ACGT'C
Cuts at: 0 173 4323
Size: 173 4150
AccI GT'mk_AC
Cuts at: 0 1991 3438 4323
Size: 1991 1447 885
Fragments arranged by size:
1991 1447 885
Acc65I G'GTAC_C
Cuts at: 0 491 4323
Size: 491 3832
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1124 2156 2603 2990 3555 3794 4323
Size: 1124 1032 447 387 565 239 529
Fragments arranged by size:
1124 1032 565 529 447 387 239
AfeI AGC'GCT
Cuts at: 0 94 4323
Size: 94 4229
AflIII A'CryG_T
Cuts at: 0 2548 4323
Size: 2548 1775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3499 4323
Size: 3499 824
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2005 2037 4323
Size: 2005 32 2286
Fragments arranged by size:
2286 2005 32
AlwNI CAG_nnn'CTG
Cuts at: 0 302 3244 4323
Size: 302 2942 1079
Fragments arranged by size:
2942 1079 302
ApoI r'AATT_y
Cuts at: 0 406 1054 1359 1470 2113 2462 2490 3322
Size: 406 648 305 111 643 349 28 832
Cuts at: 3322 4323
Size: 1001
Fragments arranged by size:
1001 832 648 643 406 349 305 111
28
AseI AT'TA_AT
Cuts at: 0 422 4323
Size: 422 3901
AvaI C'yCGr_G
Cuts at: 0 48 671 4323
Size: 48 623 3652
Fragments arranged by size:
3652 623 48
AvrII C'CTAG_G
Cuts at: 0 3979 4323
Size: 3979 344
BanI G'GyrC_C
Cuts at: 0 156 491 4323
Size: 156 335 3832
Fragments arranged by size:
3832 335 156
BanII G_rGCy'C
Cuts at: 0 209 4078 4126 4323
Size: 209 3869 48 197
Fragments arranged by size:
3869 209 197 48
BbeI G_GCGC'C
Cuts at: 0 160 4323
Size: 160 4163
BbsI GAAGACnn'nnnn_
Cuts at: 0 2287 3662 3732 4323
Size: 2287 1375 70 591
Fragments arranged by size:
2287 1375 591 70
BciVI GTATCCnnnnn_n'
Cuts at: 0 2737 4323
Size: 2737 1586
BclI T'GATC_A
Cuts at: 0 272 543 2777 4323
Size: 272 271 2234 1546
Fragments arranged by size:
2234 1546 272 271
BfrBI ATG'CAT
Cuts at: 0 569 4323
Size: 569 3754
BglI GCCn_nnn'nGGC
Cuts at: 0 3468 4323
Size: 3468 855
BglII A'GATC_T
Cuts at: 0 1644 2329 4323
Size: 1644 685 1994
Fragments arranged by size:
1994 1644 685
Bme1580I G_kGCm'C
Cuts at: 0 61 4323
Size: 61 4262
BmgBI CAC'GTC
Cuts at: 0 3694 4323
Size: 3694 629
BmtI G_CTAG'C
Cuts at: 0 3790 4323
Size: 3790 533
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2190 4323
Size: 2190 2133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 436 1586 4323
Size: 160 276 1150 2737
Fragments arranged by size:
2737 1150 276 160
Bpu10I CC'TnA_GC
Cuts at: 0 134 2708 4323
Size: 134 2574 1615
Fragments arranged by size:
2574 1615 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1525 3329 4323
Size: 1525 1804 994
Fragments arranged by size:
1804 1525 994
BsaAI yAC'GTr
Cuts at: 0 2551 4323
Size: 2551 1772
BsaBI GATnn'nnATC
Cuts at: 0 398 506 2779 3953 4323
Size: 398 108 2273 1174 370
Fragments arranged by size:
2273 1174 398 370 108
BsaHI Gr'CG_yC
Cuts at: 0 157 170 4323
Size: 157 13 4153
Fragments arranged by size:
4153 157 13
BsaWI w'CCGG_w
Cuts at: 0 3902 4022 4323
Size: 3902 120 301
Fragments arranged by size:
3902 301 120
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3119 3149 4323
Size: 3119 30 1174
Fragments arranged by size:
3119 1174 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 26 442 712 781 2089 2311 2641 2759
Size: 26 416 270 69 1308 222 330 118
Cuts at: 2759 3143 3146 3544 3556 3871 3874 3877 3934
Size: 384 3 398 12 315 3 3 57
Cuts at: 3934 3937 4273 4323
Size: 3 336 50
Fragments arranged by size:
1308 416 398 384 336 330 315 270
222 118 69 57 50 26 12 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 682 4323
Size: 682 3641
BsiHKAI G_wGCw'C
Cuts at: 0 209 4009 4251 4323
Size: 209 3800 242 72
Fragments arranged by size:
3800 242 209 72
BsmI GAATG_Cn'
Cuts at: 0 355 1951 4323
Size: 355 1596 2372
Fragments arranged by size:
2372 1596 355
BsmBI CGTCTCn'nnnn_
Cuts at: 0 454 3904 4323
Size: 454 3450 419
Fragments arranged by size:
3450 454 419
Bsp1286I G_dGCh'C
Cuts at: 0 61 209 4009 4078 4126 4251 4323
Size: 61 148 3800 69 48 125 72
Fragments arranged by size:
3800 148 125 72 69 61 48
BspHI T'CATG_A
Cuts at: 0 1305 4323
Size: 1305 3018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 122 4323
Size: 122 4201
BsrBI CCG'CTC
Cuts at: 0 144 4323
Size: 144 4179
BsrDI GCAATG_nn'
Cuts at: 0 2580 4323
Size: 2580 1743
BsrFI r'CCGG_y
Cuts at: 0 3263 4323
Size: 3263 1060
BsrGI T'GTAC_A
Cuts at: 0 524 4323
Size: 524 3799
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3244 4323
Size: 3244 1079
BstBI TT'CG_AA
Cuts at: 0 3813 4323
Size: 3813 510
BstXI CCAn_nnnn'nTGG
Cuts at: 0 460 2065 4323
Size: 460 1605 2258
Fragments arranged by size:
2258 1605 460
BstYI r'GATC_y
Cuts at: 0 993 1280 1562 1644 2329 3340 3393 4323
Size: 993 287 282 82 685 1011 53 930
Fragments arranged by size:
1011 993 930 685 287 282 82 53
Bsu36I CC'TnA_GG
Cuts at: 0 1868 4323
Size: 1868 2455
BtsI GCAGTG_nn'
Cuts at: 0 1292 2147 2351 2604 4323
Size: 1292 855 204 253 1719
Fragments arranged by size:
1719 1292 855 253 204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2670 2705 4323
Size: 2670 35 1618
Fragments arranged by size:
2670 1618 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1192 4323
Size: 1192 3131
EaeI y'GGCC_r
Cuts at: 0 1971 3138 3460 4323
Size: 1971 1167 322 863
Fragments arranged by size:
1971 1167 863 322
EarI CTCTTCn'nnn_
Cuts at: 0 561 672 809 1259 1400 1656 1694 2288
Size: 561 111 137 450 141 256 38 594
Cuts at: 2288 2420 2618 3302 3521 3676 3743 3863 4037
Size: 132 198 684 219 155 67 120 174
Cuts at: 4037 4250 4323
Size: 213 73
Fragments arranged by size:
684 594 561 450 256 219 213 198
174 155 141 137 132 120 111 73
67 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3386 4323
Size: 3386 937
EcoICRI GAG'CTC
Cuts at: 0 207 4323
Size: 207 4116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 436 1124 1586 2156 2603 2990 3555
Size: 160 276 688 462 570 447 387 565
Cuts at: 3555 3794 4323
Size: 239 529
Fragments arranged by size:
688 570 565 529 462 447 387 276
239 160
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2008 2178 4323
Size: 2008 170 2145
Fragments arranged by size:
2145 2008 170
EcoO109I rG'GnC_Cy
Cuts at: 0 1201 3984 4323
Size: 1201 2783 339
Fragments arranged by size:
2783 1201 339
EcoRI G'AATT_C
Cuts at: 0 3322 4323
Size: 3322 1001
EcoRV GAT'ATC
Cuts at: 0 2216 3153 4323
Size: 2216 937 1170
Fragments arranged by size:
2216 1170 937
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4288 4323
Size: 4288 35
HaeII r_GCGC'y
Cuts at: 0 96 160 1734 4323
Size: 96 64 1574 2589
Fragments arranged by size:
2589 1574 96 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 1554 1586 2205 2237 2361 2393
Size: 21 32 1501 32 619 32 124 32
Cuts at: 2393 2526 2558 3385 3417 3520 3552 4323
Size: 133 32 827 32 103 32 771
Fragments arranged by size:
1501 827 771 619 133 124 103 32
32 32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 0 2432 3035 4323
Size: 2432 603 1288
Fragments arranged by size:
2432 1288 603
KasI G'GCGC_C
Cuts at: 0 156 4323
Size: 156 4167
KpnI G_GTAC'C
Cuts at: 0 495 4323
Size: 495 3828
MfeI C'AATT_G
Cuts at: 0 458 496 3086 4323
Size: 458 38 2590 1237
Fragments arranged by size:
2590 1237 458 38
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1249 2203 4323
Size: 1249 954 2120
Fragments arranged by size:
2120 1249 954
MscI TGG'CCA
Cuts at: 0 1973 4323
Size: 1973 2350
MslI CAynn'nnrTG
Cuts at: 0 564 3947 4323
Size: 564 3383 376
Fragments arranged by size:
3383 564 376
MspA1I CmG'CkG
Cuts at: 0 19 105 1320 1876 3241 4323
Size: 19 86 1215 556 1365 1082
Fragments arranged by size:
1365 1215 1082 556 86 19
NaeI GCC'GGC
Cuts at: 0 3265 4323
Size: 3265 1058
NarI GG'CG_CC
Cuts at: 0 157 4323
Size: 157 4166
NgoMIV G'CCGG_C
Cuts at: 0 3263 4323
Size: 3263 1060
NheI G'CTAG_C
Cuts at: 0 3786 4323
Size: 3786 537
NsiI A_TGCA'T
Cuts at: 0 571 4323
Size: 571 3752
PmlI CAC'GTG
Cuts at: 0 2551 4323
Size: 2551 1772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 4323
Size: 112 32 4179
Fragments arranged by size:
4179 112 32
PpuMI rG'GwC_Cy
Cuts at: 0 1201 3984 4323
Size: 1201 2783 339
Fragments arranged by size:
2783 1201 339
PstI C_TGCA'G
Cuts at: 0 155 252 2616 4323
Size: 155 97 2364 1707
Fragments arranged by size:
2364 1707 155 97
PvuII CAG'CTG
Cuts at: 0 1320 1876 3241 4323
Size: 1320 556 1365 1082
Fragments arranged by size:
1365 1320 1082 556
SacI G_AGCT'C
Cuts at: 0 209 4323
Size: 209 4114
SapI GCTCTTCn'nnn_
Cuts at: 0 561 4323
Size: 561 3762
ScaI AGT'ACT
Cuts at: 0 1839 1962 4323
Size: 1839 123 2361
Fragments arranged by size:
2361 1839 123
SfcI C'TryA_G
Cuts at: 0 151 248 797 1413 2612 3060 4086 4323
Size: 151 97 549 616 1199 448 1026 237
Fragments arranged by size:
1199 1026 616 549 448 237 151 97
SfoI GGC'GCC
Cuts at: 0 158 4323
Size: 158 4165
SgrAI Cr'CCGG_yG
Cuts at: 0 3263 4323
Size: 3263 1060
SmaI CCC'GGG
Cuts at: 0 50 4323
Size: 50 4273
SmlI C'TyrA_G
Cuts at: 0 1504 3344 4323
Size: 1504 1840 979
Fragments arranged by size:
1840 1504 979
SspI AAT'ATT
Cuts at: 0 268 417 1068 2566 3511 4323
Size: 268 149 651 1498 945 812
Fragments arranged by size:
1498 945 812 651 268 149
StyI C'CwwG_G
Cuts at: 0 3979 4181 4323
Size: 3979 202 142
Fragments arranged by size:
3979 202 142
TatI w'GTAC_w
Cuts at: 0 485 524 633 1837 1954 1960 3714 4323
Size: 485 39 109 1204 117 6 1754 609
Fragments arranged by size:
1754 1204 609 485 117 109 39 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4017 4049 4215 4247 4323
Size: 4017 32 166 32 76
Fragments arranged by size:
4017 166 76 32 32
XbaI T'CTAG_A
Cuts at: 0 810 1842 2256 2344 4323
Size: 810 1032 414 88 1979
Fragments arranged by size:
1979 1032 810 414 88
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1014 2358 2792 4323
Size: 1014 1344 434 1531
Fragments arranged by size:
1531 1344 1014 434
XmaI C'CCGG_G
Cuts at: 0 48 4323
Size: 48 4275
XmnI GAAnn'nnTTC
Cuts at: 0 1826 3729 4323
Size: 1826 1903 594
Fragments arranged by size:
1903 1826 594
ZraI GAC'GTC
Cuts at: 0 171 4323
Size: 171 4152
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflIII AlfI AloI
AlwNI ApoI AseI AvaI AvrII BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI
BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstBI
BstXI BstYI Bsu36I BtsI CspCI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII KasI KpnI MfeI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NsiI PmlI
PpiI PpuMI PstI PvuII SacI SapI ScaI SfcI
SfoI SgrAI SmaI SmlI SspI StyI TatI TstI
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AgeI AhdI AleI ApaI ApaLI
AscI AsiSI BaeI BamHI BbvCI BcgI BlpI BmrI
BsaI BsgI BsiEI BsiWI BspEI BssHII BssSI BstEII
BstZ17I BtgI BtgZI ClaI DraI DraIII EagI FseI
FspI FspAI HindIII HpaI MluI NcoI NdeI NotI
NruI NspI PacI PasI PciI PflMI PfoI PmeI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI SexAI SfiI SgfI SnaBI SpeI
SphI SrfI StuI SwaI TaqII Tth111I XhoI