################################## # NO CUT ENZYMES ( 196 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI Hin1I HindIII HinfI HpaI HphI KasI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AfaI 93 GTAC GT^AC AflIII 35 ACATGT A^C[GA][CT]GT AluI 190 AGCT AG^CT BclI 20 TGATCA T^GATCA BglI 73 GCCACGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaJI 83 CCATGG C^C[ACGT][ACGT]GG BsaWI 8 ACCGGA [AT]^CCGG[AT] BseRI 250 CTCCTC GAGGAG(10/8) BsmAI 205 GAGAC GTCTC(1/5) BspLU11I 35 ACATGT A^CATGT BsrDI 111 CATTGC GCAATG(2/0) BssKI 158 CCTGG ^CC[ACGT]GG BssSI 202 CACGAG CACGAG(-5/-1) BstF5I 57 GGATG GGATG(2/0) BstNI 158 CCTGG CC^[AT]GG Cac8I 78 GCAGGC GC[ACGT]^[ACGT]GC CfoI 71 GCGC GCG^C Cfr13I 174 GGGCC G^G[ACGT]CC DdeI 41 CTGAG C^T[ACGT]AG DpnII 21 GATC ^GATC DsaI 83 CCATGG C^C[GA][CT]GG EarI 253 CTCTTC CTCTTC(1/4) EcoRII 158 CCTGG ^CC[AT]GG EcoT14I 83 CCATGG C^C[AT][AT]GG FbaI 20 TGATCA T^GATCA FokI 57 GGATG GGATG(9/13) HapII 9 CCGG C^CGG HhaI 71 GCGC GCG^C HinP1I 71 GCGC G^CGC HincII 64 GTTGAC GT[CT]^[GA]AC HindII 64 GTTGAC GT[CT]^[GA]AC HpaII 9 CCGG C^CGG Ksp632I 253 CTCTTC CTCTTC(1/4) MaeII 194 ACGT A^CGT MaeIII 196 GTCAC ^GT[ACGT]AC MboI 21 GATC ^GATC MboII 254 TCTTC GAAGA(8/7) MspI 9 CCGG C^CGG MvaI 158 CCTGG CC^[AT]GG MwoI 73 GCCACGCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 83 CCATGG C^CATGG NdeII 21 GATC ^GATC RsaI 93 GTAC GT^AC Sau3AI 21 GATC ^GATC Sau96I 174 GGGCC G^G[ACGT]CC ScrFI 158 CCTGG CC^[ACGT]GG SfaNI 58 GATGC GCATC(5/9) StyI 83 CCATGG C^C[AT][AT]GG Tsp45I 196 GTCAC ^GT[GC]AC TspRI 17 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 74 CCACGCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AcsI 133 GAATTT [GA]^AATT[CT] 180 AAATTT ApoI 133 GAATTT [GA]^AATT[CT] 180 AAATTT BbvI 188 GCAGC GCAGC(8/12) 115 GCTGC Fnu4HI 115 GCTGC GC^[ACGT]GC 188 GCAGC HaeIII 81 GGCC GG^CC 175 GGCC ItaI 115 GCTGC GC^[ACGT]GC 188 GCAGC NspI 35 ACATGT [GA]CATG^[CT] 47 ACATGC TseI 115 GCTGC G^C[AT]GC 188 GCAGC Tsp509I 134 AATT ^AATT 181 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MnlI NlaIII