(Linear) (Six-base) MAPSORT of: KIEE1583.seq  Check: 5850  from: 1  to: 267



KIEE1583.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:56  ..

AflIII A'CryG_T

Cuts at:      0      35     267 
   Size:         35     232
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      74     267 
   Size:         74     193
 
ApoI r'AATT_y

Cuts at:      0     133     180     267 
   Size:        133      47      87

  Fragments arranged by size: 

                 133      87      47
 
BclI T'GATC_A

Cuts at:      0      20     267 
   Size:         20     247
 
BglI GCCn_nnn'nGGC

Cuts at:      0      79     267 
   Size:         79     188
 
BsaWI w'CCGG_w

Cuts at:      0       8     267 
   Size:          8     259
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     241     267 
   Size:        241      26
 
BsrDI GCAATG_nn'

Cuts at:      0     110     267 
   Size:        110     157
 
BssSI C'ACGA_G

Cuts at:      0     202     267 
   Size:        202      65
 
BtgI C'CryG_G

Cuts at:      0      83     267 
   Size:         83     184
 
BtsI GCAGTG_nn'

Cuts at:      0      23     267 
   Size:         23     244
 
EarI CTCTTCn'nnn_

Cuts at:      0     259     267 
   Size:        259       8
 
HincII GTy'rAC

Cuts at:      0      66     267 
   Size:         66     201
 
NcoI C'CATG_G

Cuts at:      0      83     267 
   Size:         83     184
 
NspI r_CATG'y

Cuts at:      0      39      51     267 
   Size:         39      12     216

  Fragments arranged by size: 

                 216      39      12
 
PciI A'CATG_T

Cuts at:      0      35     267 
   Size:         35     232
 
StyI C'CwwG_G

Cuts at:      0      83     267 
   Size:         83     184
 
TatI w'GTAC_w

Cuts at:      0      92     267 
   Size:         92     175
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      81     267 
   Size:         81     186
 

 Enzymes that do cut:

   AflIII     AlfI     ApoI     BclI     BglI    BsaWI    BseRI    BsrDI
    BssSI     BtgI     BtsI     EarI   HincII     NcoI     NspI     PciI
     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI