################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DraIII EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI Hin1I HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp509I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AgeI 214 ACCGGT A^CCGGT AlwNI 293 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 571 GAGCAC G[AT]GC[AT]^C AvaI 60 CTCGGG C^[CT]CG[GA]G BclI 608 TGATCA T^GATCA BsaI 637 GAGACC GGTCTC(1/5) BsaWI 214 ACCGGT [AT]^CCGG[AT] BsiEI 176 CGATCG CG[GA][CT]^CG BsiHKAI 571 GAGCAC G[AT]GC[AT]^C BsoBI 60 CTCGGG C^[CT]CG[GA]G BsrBI 47 CCGCTC CCGCTC(-3/-3) CfoI 378 GCGC GCG^C DrdI 457 GACAAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 434 CCACGG C^C[GA][CT]GG EcoNI 417 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 608 TGATCA T^GATCA HaeII 377 GGCGCT [GA]GCGC^[CT] HgaI 130 GACGC GACGC(5/10) HhaI 378 GCGC GCG^C HinP1I 378 GCGC G^CGC HincII 466 GTCAAC GT[CT]^[GA]AC HindII 466 GTCAAC GT[CT]^[GA]AC HindIII 519 AAGCTT A^AGCTT MaeII 191 ACGT A^CGT MaeIII 218 GTGAC ^GT[ACGT]AC MboII 9 GAAGA GAAGA(8/7) MseI 272 TTAA T^TAA MslI 628 CACCCGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflMI 66 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 214 ACCGGT A^CCGGT PpuMI 356 GGGACCT [GA]G^G[AT]CC[CT] PvuI 176 CGATCG CGAT^CG SgrAI 213 CACCGGTG C[GA]^CCGG[CT]G Tru9I 272 TTAA T^TAA Tsp45I 218 GTGAC ^GT[GC]AC Van91I 66 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwI 621 GATCC GGATC(4/5) 209 GGATC AocI 235 CCTGAGG CC^T[ACGT]AGG 417 CCTGAGG ApaI 55 GGGCCC GGGCC^C 142 GGGCCC BanII 55 GGGCCC G[GA]GC[CT]^C 142 GGGCCC BglI 28 GCCGGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 174 GCCGATCGGGC BseRI 238 GAGGAG GAGGAG(10/8) 601 GAGGAG BspMI 565 ACCTGC ACCTGC(4/8) 423 GCAGGT BstF5I 546 CATCC GGATG(2/0) 666 GGATG Bsu36I 235 CCTGAGG CC^T[ACGT]AGG 417 CCTGAGG Eco81I 235 CCTGAGG CC^T[ACGT]AGG 417 CCTGAGG FauI 354 GCGGG CCCGC(4/6) 710 GCGGG FokI 546 CATCC GGATG(9/13) 666 GGATG NlaIII 285 CATG CATG^ 480 CATG PstI 160 CTGCAG CTGCA^G 202 CTGCAG SfaNI 681 GATGC GCATC(5/9) 545 GCATC TspRI 297 CACTG CA[GC]TG[ACGT][ACGT]^ 449 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AspI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstNI Cac8I Cfr10I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MboI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIV PflFI PleI Sau3AI Sau96I ScrFI SfcI TaqI TfiI TseI Tth111I TthHB8I