################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FseI FspI HaeII HgaI Hin1I HindIII KasI KpnI MaeI MaeII MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AspHI 721 GAGCTC G[AT]GC[AT]^C BalI 971 TGGCCA TGG^CCA BclI 444 TGATCA T^GATCA BsaI 562 GAGACC GGTCTC(1/5) BseRI 1030 GAGGAG GAGGAG(10/8) BsgI 117 CTGCAC GTGCAG(16/14) BsiHKAI 721 GAGCTC G[AT]GC[AT]^C BsmI 1195 GCATTC GAATGC(1/-1) BsrFI 230 ACCGGC [GA]^CCGG[CT] Cac8I 1267 GCTGGC GC[ACGT]^[ACGT]GC CfoI 879 GCGC GCG^C Cfr10I 230 ACCGGC [GA]^CCGG[CT] DraII 761 AGGGCCT [GA]G^G[ACGT]CC[CT] DrdI 1177 GACCTCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 963 GAAGAG CTCTTC(1/4) EcoNI 969 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 761 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT22I 1193 ATGCAT ATGCA^T FbaI 444 TGATCA T^GATCA HhaI 879 GCGC GCG^C HinP1I 879 GCGC G^CGC HincII 742 GTTAAC GT[CT]^[GA]AC HindII 742 GTTAAC GT[CT]^[GA]AC HpaI 742 GTTAAC GTT^AAC Ksp632I 963 GAAGAG CTCTTC(1/4) KspI 130 CCGCGG CCGC^GG MluNI 971 TGGCCA TGG^CCA MscI 971 TGGCCA TGG^CCA NcoI 935 CCATGG C^CATGG NsiI 1193 ATGCAT ATGCA^T NspI 1052 ACATGC [GA]CATG^[CT] PflMI 606 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 721 GAGCTC GAGCT^C SacII 130 CCGCGG CCGC^GG SapI 963 GAAGAGC GCTCTTC(1/4) StuI 1312 AGGCCT AGG^CCT Van91I 606 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 131 CGCG CG^CG 880 CGCG AfaI 852 GTAC GT^AC 1250 GTAC AlwI 213 GATCC GGATC(4/5) 450 GATCC AvaII 939 GGTCC G^G[AT]CC 1047 GGTCC BanI 373 GGTGCC G^G[CT][GA]CC 892 GGCACC BcnI 171 CCCGG CC^[GC]GG 216 CCCGG BpmI 391 CTCCAG CTGGAG(16/14) 604 CTCCAG BsmFI 80 GGGAC GGGAC(10/14) 134 GGGAC BsrBI 165 CCGCTC CCGCTC(-3/-3) 644 GAGCGG BstF5I 192 GGATG GGATG(2/0) 1020 GGATG BstUI 131 CGCG CG^CG 880 CGCG BstYI 212 AGATCC [GA]^GATC[CT] 449 AGATCC FokI 192 GGATG GGATG(9/13) 1020 GGATG MflI 212 AGATCC [GA]^GATC[CT] 449 AGATCC MvnI 131 CGCG CG^CG 880 CGCG NciI 171 CCCGG CC^[GC]GG 216 CCCGG PleI 482 GACTC GAGTC(4/5) 829 GAGTC PvuII 1096 CAGCTG CAG^CTG 1241 CAGCTG RsaI 852 GTAC GT^AC 1250 GTAC SmlI 904 CTTGAG C^T[CT][GA]AG 1213 CTTGAG TaqI 489 TCGA T^CGA 1118 TCGA Tsp509I 259 AATT ^AATT 1025 AATT TthHB8I 489 TCGA T^CGA 1118 TCGA XhoII 212 AGATCC [GA]^GATC[CT] 449 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AluI AlwNI BanII BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstNI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I TspRI