(Circular) (Six-base) MAPSORT of: pFN21AE1926.seq Check: 3805 from: 1 to: 6097 pFN21AE1926 6097 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3440 1937 Size: 1503 4594 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2913 3070 4160 5544 5812 1632 Size: 1281 157 1090 1384 268 1917 Fragments arranged by size: 1917 1384 1281 1090 268 157 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 4996 5973 1203 Size: 3793 977 1327 Fragments arranged by size: 3793 1327 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 2903 1166 Size: 1737 4360 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2921 1184 Size: 1737 4360 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2202 2234 2855 2887 2202 Size: 32 621 32 5412 Fragments arranged by size: 5412 621 32 32 AlwNI CAG_nnn'CTG Cuts at: 2296 2404 2617 3840 5412 2296 Size: 108 213 1223 1572 2981 Fragments arranged by size: 2981 1572 1223 213 108 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 3406 3632 3784 5893 1493 Size: 1913 226 152 2109 1697 Fragments arranged by size: 2109 1913 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 3422 1870 Size: 73 1479 4545 Fragments arranged by size: 4545 1479 73 BamHI G'GATC_C Cuts at: 3427 3794 3427 Size: 367 5730 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2378 2897 3418 4873 618 Size: 325 217 627 591 519 521 1455 1842 Fragments arranged by size: 1842 1455 627 591 521 519 325 217 BanII G_rGCy'C Cuts at: 1391 2111 2207 2730 3416 1391 Size: 720 96 523 686 4072 Fragments arranged by size: 4072 720 686 523 96 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5126 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4288 4322 1903 Size: 34 2351 34 3678 Fragments arranged by size: 3678 2351 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3989 5199 3989 Size: 1210 4887 BclI T'GATC_A Cuts at: 1548 2449 1548 Size: 901 5196 BfrBI ATG'CAT Cuts at: 3200 3200 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4708 136 Size: 107 122 71 631 605 3036 1525 Fragments arranged by size: 3036 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6092 6092 Size: 6097 BlpI GC'TnA_GC Cuts at: 3514 3514 Size: 6097 Bme1580I G_kGCm'C Cuts at: 1790 4158 5314 1790 Size: 2368 1156 2573 Fragments arranged by size: 2573 2368 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6097 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3828 4786 442 Size: 416 53 83 399 24 2411 958 1753 Fragments arranged by size: 2411 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2624 3200 2624 Size: 576 5521 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 2594 4757 1239 Size: 1142 213 2163 2579 Fragments arranged by size: 2579 2163 1142 213 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2930 3239 4222 5087 5385 2930 Size: 309 983 865 298 3642 Fragments arranged by size: 3642 983 865 309 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2561 4760 882 Size: 617 1062 2199 2219 Fragments arranged by size: 2219 2199 1062 617 BsaAI yAC'GTr Cuts at: 493 1204 3888 493 Size: 711 2684 2702 Fragments arranged by size: 2702 2684 711 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3904 4286 4921 275 Size: 53 83 186 936 2371 382 635 1451 Fragments arranged by size: 2371 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4530 4928 5202 5349 5760 4530 Size: 398 274 147 411 4867 Fragments arranged by size: 4867 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2495 2525 1178 Size: 30 165 30 1092 30 4750 Fragments arranged by size: 4750 1092 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3050 1186 Size: 1864 4233 BseYI C'CCAG_C Cuts at: 2374 5300 5796 2374 Size: 2926 496 2675 Fragments arranged by size: 2926 2675 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2107 1163 Size: 573 371 5153 Fragments arranged by size: 5153 573 371 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3547 4308 5336 1540 Size: 275 186 1546 761 1028 2301 Fragments arranged by size: 2301 1546 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2730 4158 4243 5314 2730 Size: 1428 85 1071 3513 Fragments arranged by size: 3513 1428 1071 85 BsmI GAATG_Cn' Cuts at: 3200 3608 3701 3200 Size: 408 93 5596 Fragments arranged by size: 5596 408 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2111 2207 2730 3416 4158 4243 5314 Size: 399 321 96 523 686 742 85 1071 Cuts at: 5314 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 686 523 399 321 96 85 BspEI T'CCGG_A Cuts at: 5760 5760 Size: 6097 BspHI T'CATG_A Cuts at: 3986 5716 3986 Size: 1730 4367 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3452 844 Size: 2608 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2172 2651 3984 1394 Size: 429 349 479 1333 3507 Fragments arranged by size: 3507 1333 479 429 349 BsrDI GCAATG_nn' Cuts at: 65 1248 4592 4766 65 Size: 1183 3344 174 1396 Fragments arranged by size: 3344 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2235 4741 4928 1700 Size: 110 425 2506 187 2869 Fragments arranged by size: 2869 2506 425 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6097 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6097 BstYI r'GATC_y Cuts at: 1972 2217 2454 3427 3794 4179 4196 5648 5734 Size: 245 237 973 367 385 17 1452 86 Cuts at: 5734 5746 6092 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 973 385 367 346 245 237 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2135 2352 2940 513 Size: 552 1070 217 588 3670 Fragments arranged by size: 3670 1070 588 552 217 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 3244 3613 4407 4427 3244 Size: 369 794 20 4914 Fragments arranged by size: 4914 794 369 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2341 2376 529 Size: 35 1777 35 4250 Fragments arranged by size: 4250 1777 35 35 DraI TTT'AAA Cuts at: 3401 3401 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3188 5104 809 Size: 507 1872 1916 1802 Fragments arranged by size: 1916 1872 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2652 2976 3274 Size: 54 1425 50 275 18 822 324 298 Cuts at: 3274 3544 4433 6062 8 Size: 270 889 1629 43 Fragments arranged by size: 1629 1425 889 822 324 298 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3544 1537 Size: 275 1732 4090 Fragments arranged by size: 4090 1732 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2963 4027 1353 Size: 1610 1064 3423 Fragments arranged by size: 3423 1610 1064 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4684 5058 5204 387 Size: 1064 138 3095 374 146 1280 Fragments arranged by size: 3095 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2728 2728 Size: 6097 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 2594 2913 3070 4160 4757 5544 Size: 393 749 213 319 157 1090 597 787 Cuts at: 5544 5812 1239 Size: 268 1524 Fragments arranged by size: 1524 1090 787 749 597 393 319 268 213 157 EcoNI CCTnn'n_nnAGG Cuts at: 2978 2978 Size: 6097 EcoO109I rG'GnC_Cy Cuts at: 2767 2767 Size: 6097 EcoRI G'AATT_C Cuts at: 3406 3784 3406 Size: 378 5719 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 5244 1678 Size: 3566 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2189 2221 2417 2429 2449 Size: 32 135 32 617 32 196 12 20 Cuts at: 2449 2461 2640 2672 2819 2851 4738 4770 4812 Size: 12 179 32 147 32 1887 32 42 Cuts at: 4812 4844 1373 Size: 32 2626 Fragments arranged by size: 2626 1887 617 196 179 147 135 42 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2749 3441 3687 669 Size: 988 281 811 692 246 3079 Fragments arranged by size: 3079 988 811 692 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6097 HpaI GTT'AAC Cuts at: 2749 3687 2749 Size: 938 5159 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5211 5395 1305 Size: 3906 184 2007 Fragments arranged by size: 3906 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2978 10 Size: 54 1425 343 1146 3129 Fragments arranged by size: 3129 1425 1146 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2903 2939 4055 4414 4573 518 Size: 648 981 756 36 1116 359 159 2042 Fragments arranged by size: 2042 1116 981 756 648 359 159 36 MspA1I CmG'CkG Cuts at: 1606 2137 3103 3248 3260 3513 4190 5338 5583 Size: 531 966 145 12 253 677 1148 245 Cuts at: 5583 1606 Size: 2120 Fragments arranged by size: 2120 1148 966 677 531 253 245 145 12 NcoI C'CATG_G Cuts at: 513 1065 2940 513 Size: 552 1875 3670 Fragments arranged by size: 3670 1875 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NsiI A_TGCA'T Cuts at: 3202 3202 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 3061 3455 5000 1132 Size: 97 1832 394 1545 2229 Fragments arranged by size: 2229 1832 1545 394 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6097 PciI A'CATG_T Cuts at: 4996 4996 Size: 6097 PflMI CCAn_nnn'nTGG Cuts at: 1108 2617 1108 Size: 1509 4588 PfoI T'CCnGG_A Cuts at: 2220 2220 Size: 6097 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4166 4198 5679 5711 4166 Size: 32 1481 32 4552 Fragments arranged by size: 4552 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 3335 3667 3335 Size: 332 5765 PstI C_TGCA'G Cuts at: 830 3449 830 Size: 2619 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 PvuII CAG'CTG Cuts at: 3103 3248 3103 Size: 145 5952 SacI G_AGCT'C Cuts at: 2730 2730 Size: 6097 SacII CC_GC'GG Cuts at: 2138 2138 Size: 6097 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SapI GCTCTTCn'nnn_ Cuts at: 2963 2963 Size: 6097 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SfcI C'TryA_G Cuts at: 826 1045 3445 4580 5261 5452 826 Size: 219 2400 1135 681 191 1471 Fragments arranged by size: 2400 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 3424 1872 Size: 1552 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2909 3218 4201 5102 5364 820 Size: 197 926 966 309 983 901 262 1553 Fragments arranged by size: 1553 983 966 926 901 309 262 197 SnaBI TAC'GTA Cuts at: 493 3888 493 Size: 3395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 3455 1132 Size: 2323 3774 SspI AAT'ATT Cuts at: 5 52 4021 5 Size: 47 3969 2081 Fragments arranged by size: 3969 2081 47 StuI AGG'CCT Cuts at: 1517 3319 1517 Size: 1802 4295 StyI C'CwwG_G Cuts at: 513 1065 2256 2940 2979 3536 513 Size: 552 1191 684 39 557 3074 Fragments arranged by size: 3074 1191 684 557 552 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2366 2656 2696 2915 4124 4141 4294 Size: 1255 786 290 40 219 1209 17 153 Cuts at: 4294 4479 4924 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1209 786 445 290 219 185 153 40 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4343 6055 96 Size: 274 80 33 51 1443 2366 1712 138 Fragments arranged by size: 2366 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6057 6089 6057 Size: 32 6065 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6097 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 3422 1870 Size: 1552 4545 XmnI GAAnn'nnTTC Cuts at: 4226 4226 Size: 6097 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI