Restriction map for
pFN21AE1793
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tth111I TthHB8I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 496 CGCG CG^CG
AcsI 244 AAATTC [GA]^AATT[CT]
AfaI 490 GTAC GT^AC
ApaI 531 GGGCCC GGGCC^C
ApoI 244 AAATTC [GA]^AATT[CT]
AspHI 310 GAGCTC G[AT]GC[AT]^C
AvaI 405 CTCGGG C^[CT]CG[GA]G
BanI 694 GGCACC G^G[CT][GA]CC
BciVI 499 GGATAC GTATCC(6/5)
BcnI 255 CCCGG CC^[GC]GG
BlpI 831 GCTCAGC GC^T[ACGT]AGC
BpmI 724 CTCCAG CTGGAG(16/14)
Bpu1102I 831 GCTCAGC GC^T[ACGT]AGC
BsaAI 487 CACGTA [CT]AC^GT[GA]
BsgI 178 CTGCAC GTGCAG(16/14)
BsiHKAI 310 GAGCTC G[AT]GC[AT]^C
BsmAI 715 GAGAC GTCTC(1/5)
BsmBI 715 GAGACG CGTCTC(1/5)
BsoBI 405 CTCGGG C^[CT]CG[GA]G
BsrBI 226 GAGCGG CCGCTC(-3/-3)
BstEII 417 GGTGACC G^GT[ACGT]ACC
BstPI 417 GGTGACC G^GT[ACGT]ACC
BstUI 496 CGCG CG^CG
CelII 831 GCTCAGC GC^T[ACGT]AGC
DraII 531 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 203 CCGTGG C^C[GA][CT]GG
EarI 840 GAAGAG CTCTTC(1/4)
EcoO109I 531 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 417 GGTGACC G^GT[ACGT]ACC
FauI 269 CCCGC CCCGC(4/6)
HaeII 58 AGCGCC [GA]GCGC^[CT]
HapII 256 CCGG C^CGG
HgaI 29 GCGTC GACGC(5/10)
HpaII 256 CCGG C^CGG
HphI 417 GGTGA GGTGA(8/7)
Ksp632I 840 GAAGAG CTCTTC(1/4)
MaeII 488 ACGT A^CGT
MslI 560 CATACCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 256 CCGG C^CGG
MvnI 496 CGCG CG^CG
NciI 255 CCCGG CC^[GC]GG
NspI 452 GCATGT [GA]CATG^[CT]
PflMI 105 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 680 CTGCAG CTGCA^G
RsaI 490 GTAC GT^AC
SacI 310 GAGCTC GAGCT^C
SfaNI 293 GCATC GCATC(5/9)
SfcI 680 CTGCAG C^T[GA][CT]AG
SmlI 466 CTCAAG C^T[CT][GA]AG
TfiI 762 GAATC G^A[AT]TC
Van91I 105 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 435 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AvaII 391 GGACC G^G[AT]CC
510 GGTCC
BsmFI 556 GGGAC GGGAC(10/14)
720 GGGAC
BstYI 339 GGATCT [GA]^GATC[CT]
478 AGATCC
Cac8I 855 GCGAGC GC[ACGT]^[ACGT]GC
859 GCCAGC
CfoI 59 GCGC GCG^C
495 GCGC
HhaI 59 GCGC GCG^C
495 GCGC
HinP1I 59 GCGC G^CGC
495 GCGC
MflI 339 GGATCT [GA]^GATC[CT]
478 AGATCC
MseI 233 TTAA T^TAA
706 TTAA
MspA1I 175 CAGCTG C[AC]G^C[GT]G
288 CAGCTG
MwoI 612 GCCAGTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
859 GCCAGCAGAGC
PvuII 175 CAGCTG CAG^CTG
288 CAGCTG
Tru9I 233 TTAA T^TAA
706 TTAA
Tsp509I 154 AATT ^AATT
245 AATT
XcmI 147 CCAGGGAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
326 CCAGCAACTTGCTGG
XhoII 339 GGATCT [GA]^GATC[CT]
478 AGATCC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwI BanII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MboI MboII
MnlI MvaI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI TseI
Tsp45I TspRI