################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII CpoI DpnII DraI DraIII DrdI DsaI Eam1105I EarI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HinfI HpaI KasI Ksp632I KspI MaeI MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MunI NarI NcoI NdeI NdeII NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 94 GGTACC G^GTACC AfaI 95 GTAC GT^AC AocI 69 CCTCAGG CC^T[ACGT]AGG Aor51HI 308 AGCGCT AGC^GCT Asp718I 94 GGTACC G^GTACC AvaII 351 GGACC G^G[AT]CC BanII 505 GGGCTC G[GA]GC[CT]^C BcgI 423 CGAGACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsiEI 274 CGGCCG CG[GA][CT]^CG BsmAI 424 GAGAC GTCTC(1/5) BsmFI 553 GTCCC GGGAC(10/14) BspDI 403 ATCGAT AT^CGAT BsrFI 479 GCCGGC [GA]^CCGG[CT] BsrI 258 CCAGT ACTGG(1/-1) BssSI 235 CACGAG CACGAG(-5/-1) BstAPI 177 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 69 CCTCAGG CC^T[ACGT]AGG Cfr10I 479 GCCGGC [GA]^CCGG[CT] ClaI 403 ATCGAT AT^CGAT EagI 274 CGGCCG C^GGCCG EclXI 274 CGGCCG C^GGCCG Eco47III 308 AGCGCT AGC^GCT Eco52I 274 CGGCCG C^GGCCG Eco81I 69 CCTCAGG CC^T[ACGT]AGG FauI 491 CCCGC CCCGC(4/6) HaeII 308 AGCGCT [GA]GCGC^[CT] HgaI 3 GACGC GACGC(5/10) HphI 319 TCACC GGTGA(8/7) KpnI 94 GGTACC GGTAC^C MaeII 551 ACGT A^CGT MaeIII 89 GTCAC ^GT[ACGT]AC MslI 567 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 428 CAGCTG C[AC]G^C[GT]G NaeI 479 GCCGGC GCC^GGC NgoMIV 479 GCCGGC G^CCGGC NspI 43 ACATGC [GA]CATG^[CT] PaeR7I 158 CTCGAG C^TCGAG PvuII 428 CAGCTG CAG^CTG RsaI 95 GTAC GT^AC SfaNI 406 GATGC GCATC(5/9) SfcI 188 CTGTAG C^T[GA][CT]AG SmlI 158 CTCGAG C^T[CT][GA]AG Tsp45I 89 GTCAC ^GT[GC]AC XhoI 158 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccII 421 CGCG CG^CG 493 CGCG AvaI 158 CTCGAG C^[CT]CG[GA]G 501 CTCGGG BbvCI 245 CCTCAGC CCTCAGC(-5/-2) 115 GCTGAGG BcnI 13 CCCGG CC^[GC]GG 264 CCCGG BseRI 7 CTCCTC GAGGAG(10/8) 280 GAGGAG BsoBI 158 CTCGAG C^[CT]CG[GA]G 501 CTCGGG BspMI 149 ACCTGC ACCTGC(4/8) 568 ACCTGC BstF5I 546 CATCC GGATG(2/0) 345 GGATG BstUI 421 CGCG CG^CG 493 CGCG EaeI 132 CGGCCA [CT]^GGCC[GA] 274 CGGCCG EcoNI 65 CCTCCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 129 CCTCGGCCAGG FokI 546 CATCC GGATG(9/13) 345 GGATG MvnI 421 CGCG CG^CG 493 CGCG NciI 13 CCCGG CC^[GC]GG 264 CCCGG NlaIII 44 CATG CATG^ 58 CATG TaqI 159 TCGA T^CGA 404 TCGA TthHB8I 159 TCGA T^CGA 404 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AluI AlwNI BanI BbvI BmyI BpmI BsaJI BsgI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspI MvaI MwoI NlaIV Sau96I ScrFI TseI TspRI