Restriction map for
pFN21AE1685
(Circular) (Six-base) MAPSORT of: pFN21AE1685.seq Check: 8122 from: 1 to: 5296
pFN21AE1685 5296 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2163 2582 2163
Size: 419 4877
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3106 278
Size: 53 83 186 936 1570 2468
Fragments arranged by size:
2468 1570 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2639 1937
Size: 702 4594
Acc65I G'GTAC_C
Cuts at: 2099 2617 2099
Size: 518 4778
AclI AA'CG_TT
Cuts at: 1563 3423 3796 1563
Size: 1860 373 3063
Fragments arranged by size:
3063 1860 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3359 4743 5011 1632
Size: 1727 1384 268 1917
Fragments arranged by size:
1917 1727 1384 268
AfeI AGC'GCT
Cuts at: 2315 2315
Size: 5296
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5099
AflIII A'CryG_T
Cuts at: 1203 4195 5172 1203
Size: 2992 977 1327
Fragments arranged by size:
2992 1327 977
AgeI A'CCGG_T
Cuts at: 4127 4127
Size: 5296
AhdI GACnn_n'nnGTC
Cuts at: 4025 4025
Size: 5296
AleI CACnn'nnGTG
Cuts at: 1166 2576 1166
Size: 1410 3886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2594 1184
Size: 1410 3886
AlwNI CAG_nnn'CTG
Cuts at: 2374 2403 2467 3039 4611 2374
Size: 29 64 572 1572 3059
Fragments arranged by size:
3059 1572 572 64 29
ApaLI G'TGCA_C
Cuts at: 3353 4509 3353
Size: 1156 4140
ApoI r'AATT_y
Cuts at: 1493 2605 2831 2983 5092 1493
Size: 1112 226 152 2109 1697
Fragments arranged by size:
2109 1697 1112 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3850 160
Size: 872 2818 1606
Fragments arranged by size:
2818 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5296
AvaI C'yCGr_G
Cuts at: 1870 1943 2163 2506 2621 1870
Size: 73 220 343 115 4545
Fragments arranged by size:
4545 343 220 115 73
BamHI G'GATC_C
Cuts at: 2626 2993 2626
Size: 367 4929
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2082 2099 2168 2617 4072
Size: 325 217 627 295 17 69 449 1455
Cuts at: 4072 618
Size: 1842
Fragments arranged by size:
1842 1455 627 449 325 295 217 69
17
BanII G_rGCy'C
Cuts at: 1391 2514 2615 1391
Size: 1123 101 4072
Fragments arranged by size:
4072 1123 101
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4325
BbvCI CC'TCA_GC
Cuts at: 2121 2251 2121
Size: 130 5166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2417 2451 3487 3521 1903
Size: 34 480 34 1036 34 3678
Fragments arranged by size:
3678 1036 480 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3188 4398 3188
Size: 1210 4086
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5296
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3907 136
Size: 107 122 71 631 605 2235 1525
Fragments arranged by size:
2235 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5291 5291
Size: 5296
BlpI GC'TnA_GC
Cuts at: 2713 2713
Size: 5296
Bme1580I G_kGCm'C
Cuts at: 1790 2085 2270 3357 4513 1790
Size: 295 185 1087 1156 2573
Fragments arranged by size:
2573 1156 1087 295 185
BmgBI CAC'GTC
Cuts at: 1318 2557 1318
Size: 1239 4057
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2257 3027 3985
Size: 416 53 83 399 24 840 770 958
Cuts at: 3985 442
Size: 1753
Fragments arranged by size:
1753 958 840 770 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5296
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 5296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2350 2444 3956 1239
Size: 995 116 94 1512 2579
Fragments arranged by size:
2579 1512 995 116 94
Bpu10I CC'TnA_GC
Cuts at: 2121 2251 2121
Size: 130 5166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3421 4286 4584 3421
Size: 865 298 4133
Fragments arranged by size:
4133 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3959 882
Size: 617 2460 2219
Fragments arranged by size:
2460 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3087 493
Size: 711 1883 2702
Fragments arranged by size:
2702 1883 711
BsaBI GATnn'nnATC
Cuts at: 5290 5290
Size: 5296
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3103 3485 4120 275
Size: 53 83 186 936 1570 382 635 1451
Fragments arranged by size:
1570 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3729 4127 4401 4548 4959 3729
Size: 398 274 147 411 4066
Fragments arranged by size:
4066 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2276 2300 2306 2323 2330
Size: 30 165 30 873 24 6 17 7
Cuts at: 2330 2353 1178
Size: 23 4121
Fragments arranged by size:
4121 873 165 30 30 24 23 17
7 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2003 2300 1186
Size: 817 297 4182
Fragments arranged by size:
4182 817 297
BseYI C'CCAG_C
Cuts at: 4499 4995 4499
Size: 496 4800
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2027 2416 2554 1163
Size: 573 291 389 138 3905
Fragments arranged by size:
3905 573 389 291 138
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2282 2746 3507 4535 1540
Size: 275 186 281 464 761 1028 2301
Fragments arranged by size:
2301 1028 761 464 281 275 186
BsiHKAI G_wGCw'C
Cuts at: 3357 3442 4513 3357
Size: 85 1071 4140
Fragments arranged by size:
4140 1071 85
BsmI GAATG_Cn'
Cuts at: 2807 2900 2807
Size: 93 5203
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2270 2514 2615 3357 3442 4513
Size: 399 295 185 244 101 742 85 1071
Cuts at: 4513 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 295 244 185 101
85
BspEI T'CCGG_A
Cuts at: 4959 4959
Size: 5296
BspHI T'CATG_A
Cuts at: 3185 4915 3185
Size: 1730 3566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 2582 2651 844
Size: 1319 419 69 3489
Fragments arranged by size:
3489 1319 419 69
BsrBI CCG'CTC
Cuts at: 1394 1823 3183 1394
Size: 429 1360 3507
Fragments arranged by size:
3507 1360 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3791 3965 65
Size: 1183 2543 174 1396
Fragments arranged by size:
2543 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2484 3940 4127 1700
Size: 110 674 1456 187 2869
Fragments arranged by size:
2869 1456 674 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5296
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5296
BssSI C'ACGA_G
Cuts at: 2240 3356 4368 2240
Size: 1116 1012 3168
Fragments arranged by size:
3168 1116 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2188 794
Size: 1394 3902
BstBI TT'CG_AA
Cuts at: 4100 4100
Size: 5296
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5296
BstYI r'GATC_y
Cuts at: 1972 2626 2993 3378 3395 4847 4933 4945 5291
Size: 654 367 385 17 1452 86 12 346
Cuts at: 5291 1972
Size: 1977
Fragments arranged by size:
1977 1452 654 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2075 2075
Size: 5296
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4744
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4557
Fragments arranged by size:
4557 683 56
BtsI GCAGTG_nn'
Cuts at: 2107 2812 3606 3626 2107
Size: 705 794 20 3777
Fragments arranged by size:
3777 794 705 20
ClaI AT'CG_AT
Cuts at: 1554 2409 1554
Size: 855 4441
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5261
DraI TTT'AAA
Cuts at: 2600 2600
Size: 5296
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4303 809
Size: 507 2987 1802
Fragments arranged by size:
2987 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 2279 2743
Size: 54 1425 50 275 18 307 142 464
Cuts at: 2743 3632 5261 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 464 307 275 142 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2279 2743 1537
Size: 275 467 464 4090
Fragments arranged by size:
4090 467 464 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3226 1353
Size: 1873 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3883 4257 4403 387
Size: 1064 138 2294 374 146 1280
Fragments arranged by size:
2294 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2350 2444 3359 3956 4743 5011
Size: 393 602 116 94 915 597 787 268
Cuts at: 5011 1239
Size: 1524
Fragments arranged by size:
1524 915 787 602 597 393 268 116
94
EcoNI CCTnn'n_nnAGG
Cuts at: 2074 2138 2074
Size: 64 5232
EcoO109I rG'GnC_Cy
Cuts at: 2143 2247 2526 2143
Size: 104 279 4913
Fragments arranged by size:
4913 279 104
EcoRI G'AATT_C
Cuts at: 2605 2983 2605
Size: 378 4918
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 5296
FspI TGC'GCA
Cuts at: 3802 3802
Size: 5296
HaeII r_GCGC'y
Cuts at: 1678 2317 4443 1678
Size: 639 2126 2531
Fragments arranged by size:
2531 2126 639
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2391 2423 3937 3969 4011
Size: 32 135 32 819 32 1514 32 42
Cuts at: 4011 4043 1373
Size: 32 2626
Fragments arranged by size:
2626 1514 819 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2640 2886 669
Size: 988 281 702 246 3079
Fragments arranged by size:
3079 988 702 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5296
HpaI GTT'AAC
Cuts at: 2886 2886
Size: 5296
KpnI G_GTAC'C
Cuts at: 2103 2621 2103
Size: 518 4778
MfeI C'AATT_G
Cuts at: 2895 2895
Size: 5296
MluI A'CGCG_T
Cuts at: 5172 5172
Size: 5296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 4410 4594 1305
Size: 969 2136 184 2007
Fragments arranged by size:
2136 2007 969 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3474
Fragments arranged by size:
3474 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2576 3254 3613 3772 518
Size: 648 1410 678 359 159 2042
Fragments arranged by size:
2042 1410 678 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2435 2712 3389 4537 4782 1606
Size: 829 277 677 1148 245 2120
Fragments arranged by size:
2120 1148 829 677 277 245
NaeI GCC'GGC
Cuts at: 2486 2486
Size: 5296
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4744
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5296
NgoMIV G'CCGG_C
Cuts at: 2484 2484
Size: 5296
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5296
NotI GC'GGCC_GC
Cuts at: 2743 2743
Size: 5296
NspI r_CATG'y
Cuts at: 1132 1229 2052 2654 4199 1132
Size: 97 823 602 1545 2229
Fragments arranged by size:
2229 1545 823 602 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5296
PciI A'CATG_T
Cuts at: 4195 4195
Size: 5296
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5296
PmeI GTTT'AAAC
Cuts at: 2600 2600
Size: 5296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2118 2150 3365 3397 4878 4910 2118
Size: 32 1215 32 1481 32 2504
Fragments arranged by size:
2504 1481 1215 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5296
PsiI TTA'TAA
Cuts at: 2866 2866
Size: 5296
PspXI vC'TCGA_Gb
Cuts at: 2163 2163
Size: 5296
PstI C_TGCA'G
Cuts at: 830 2648 830
Size: 1818 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5296
PvuII CAG'CTG
Cuts at: 2435 2435
Size: 5296
SalI G'TCGA_C
Cuts at: 1936 2638 1936
Size: 702 4594
SbfI CC_TGCA'GG
Cuts at: 2648 2648
Size: 5296
ScaI AGT'ACT
Cuts at: 3544 5256 3544
Size: 1712 3584
SfcI C'TryA_G
Cuts at: 826 1045 2193 2644 3779 4460 4651 826
Size: 219 1148 451 1135 681 191 1471
Fragments arranged by size:
1471 1148 1135 681 451 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5296
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5296
SmaI CCC'GGG
Cuts at: 1872 2623 1872
Size: 751 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2163 3400 4301 4563 820
Size: 197 926 220 1237 901 262 1553
Fragments arranged by size:
1553 1237 926 901 262 220 197
SnaBI TAC'GTA
Cuts at: 493 3087 493
Size: 2594 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5296
SphI G_CATG'C
Cuts at: 1132 2654 1132
Size: 1522 3774
SspI AAT'ATT
Cuts at: 5 52 3220 5
Size: 47 3168 2081
Fragments arranged by size:
3168 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5296
StyI C'CwwG_G
Cuts at: 513 1065 2735 513
Size: 552 1670 3074
Fragments arranged by size:
3074 1670 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 3323 3340 3493 3678 4123 325
Size: 1255 741 1002 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1002 741 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3542 5254 96
Size: 274 80 33 51 1443 1565 1712 138
Fragments arranged by size:
1712 1565 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2300 2323 2332 2355 5256 5288 2300
Size: 23 9 23 2901 32 2308
Fragments arranged by size:
2901 2308 32 23 23 9
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5296
XbaI T'CTAG_A
Cuts at: 2632 2632
Size: 5296
XhoI C'TCGA_G
Cuts at: 1943 2163 1943
Size: 220 5076
XmaI C'CCGG_G
Cuts at: 1870 2621 1870
Size: 751 4545
XmnI GAAnn'nnTTC
Cuts at: 3425 3425
Size: 5296
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3104 276
Size: 53 83 186 936 1570 2468
Fragments arranged by size:
2468 1570 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI