(Circular) (Six-base) MAPSORT of: pFN21AE9260.seq Check: 9359 from: 1 to: 9451 pFN21AE9260 9451 bp With 182 enzymes: SgfI * February 10, 2017 15:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2611 6597 7261 278 Size: 53 83 186 936 1075 3986 664 2468 Fragments arranged by size: 3986 2468 1075 936 664 186 83 53 AccI GT'mk_AC Cuts at: 1937 6092 6794 1937 Size: 4155 702 4594 Fragments arranged by size: 4594 4155 702 Acc65I G'GTAC_C Cuts at: 6772 6772 Size: 9451 AclI AA'CG_TT Cuts at: 1563 7578 7951 1563 Size: 6015 373 3063 Fragments arranged by size: 6015 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2547 3335 3492 4592 5388 5498 5860 6023 Size: 915 788 157 1100 796 110 362 163 Cuts at: 6023 7514 8898 9166 1632 Size: 1491 1384 268 1917 Fragments arranged by size: 1917 1491 1384 1100 915 796 788 362 268 163 157 110 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9254 AflIII A'CryG_T Cuts at: 1203 5469 5684 8350 9327 1203 Size: 4266 215 2666 977 1327 Fragments arranged by size: 4266 2666 1327 977 215 AgeI A'CCGG_T Cuts at: 8282 8282 Size: 9451 AhdI GACnn_n'nnGTC Cuts at: 6434 6593 8180 6434 Size: 159 1587 7705 Fragments arranged by size: 7705 1587 159 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9451 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9451 AlwNI CAG_nnn'CTG Cuts at: 2525 2635 6574 7194 8766 2525 Size: 110 3939 620 1572 3210 Fragments arranged by size: 3939 3210 1572 620 110 ApaI G_GGCC'C Cuts at: 3884 6658 3884 Size: 2774 6677 ApaLI G'TGCA_C Cuts at: 7508 8664 7508 Size: 1156 8295 ApoI r'AATT_y Cuts at: 1493 2824 3361 4128 4422 4626 5036 5094 5813 Size: 1331 537 767 294 204 410 58 719 Cuts at: 5813 5865 5948 6760 6986 7138 9247 1493 Size: 52 83 812 226 152 2109 1697 Fragments arranged by size: 2109 1697 1331 812 767 719 537 410 294 226 204 152 83 58 52 AseI AT'TA_AT Cuts at: 160 1032 8005 160 Size: 872 6973 1606 Fragments arranged by size: 6973 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9451 AvaI C'yCGr_G Cuts at: 1870 1943 3603 5265 6776 1870 Size: 73 1660 1662 1511 4545 Fragments arranged by size: 4545 1662 1660 1511 73 BamHI G'GATC_C Cuts at: 4435 6781 7148 4435 Size: 2346 367 6738 Fragments arranged by size: 6738 2346 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2070 2227 6394 6684 6772 Size: 325 217 627 283 157 4167 290 88 Cuts at: 6772 8227 618 Size: 1455 1842 Fragments arranged by size: 4167 1842 1455 627 325 290 283 217 157 88 BanII G_rGCy'C Cuts at: 1391 2217 3818 3884 5622 6231 6658 6770 1391 Size: 826 1601 66 1738 609 427 112 4072 Fragments arranged by size: 4072 1738 1601 826 609 427 112 66 BbeI G_GCGC'C Cuts at: 6688 6688 Size: 9451 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2885 3631 3774 5376 5488 6592 928 Size: 971 986 746 143 1602 112 1104 3787 Fragments arranged by size: 3787 1602 1104 986 971 746 143 112 BbvCI CC'TCA_GC Cuts at: 6033 6033 Size: 9451 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7642 7676 1903 Size: 34 5705 34 3678 Fragments arranged by size: 5705 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2706 7343 8553 2706 Size: 4637 1210 3604 Fragments arranged by size: 4637 3604 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9451 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4827 6385 8062 Size: 107 122 71 631 605 3155 1558 1677 Cuts at: 8062 136 Size: 1525 Fragments arranged by size: 3155 1677 1558 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2689 2761 4073 9446 2689 Size: 72 1312 5373 2694 Fragments arranged by size: 5373 2694 1312 72 BlpI GC'TnA_GC Cuts at: 6230 6868 6230 Size: 638 8813 Bme1580I G_kGCm'C Cuts at: 1790 2073 2230 2519 3399 3884 6658 7512 8668 Size: 283 157 289 880 485 2774 854 1156 Cuts at: 8668 1790 Size: 2573 Fragments arranged by size: 2774 2573 1156 880 854 485 289 283 157 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9451 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 7182 8140 442 Size: 416 53 83 399 24 5765 958 1753 Fragments arranged by size: 5765 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9451 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2818 3832 2818 Size: 1014 8437 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2523 2582 3866 4313 6315 6366 8111 1239 Size: 1284 59 1284 447 2002 51 1745 2579 Fragments arranged by size: 2579 2002 1745 1284 1284 447 59 51 Bpu10I CC'TnA_GC Cuts at: 6033 6033 Size: 9451 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2678 2790 3842 3905 6284 7576 8441 8739 2678 Size: 112 1052 63 2379 1292 865 298 3390 Fragments arranged by size: 3390 2379 1292 1052 865 298 112 63 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4281 8114 882 Size: 617 2782 3833 2219 Fragments arranged by size: 3833 2782 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 7242 493 Size: 711 6038 2702 Fragments arranged by size: 6038 2702 711 BsaBI GATnn'nnATC Cuts at: 2291 2760 9445 2291 Size: 469 6685 2297 Fragments arranged by size: 6685 2297 469 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2608 6594 6685 7258 Size: 53 83 186 936 1075 3986 91 573 Cuts at: 7258 7640 8275 275 Size: 382 635 1451 Fragments arranged by size: 3986 1451 1075 936 635 573 382 186 91 83 53 BsaWI w'CCGG_w Cuts at: 3786 7884 8282 8556 8703 9114 3786 Size: 4098 398 274 147 411 4123 Fragments arranged by size: 4123 4098 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4878 4908 5714 5744 1178 Size: 30 165 30 3475 30 806 30 4885 Fragments arranged by size: 4885 3475 806 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2125 2756 3824 1186 Size: 939 631 1068 6813 Fragments arranged by size: 6813 1068 939 631 BseYI C'CCAG_C Cuts at: 3877 6486 8654 9150 3877 Size: 2609 2168 496 4178 Fragments arranged by size: 4178 2609 2168 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3078 1163 Size: 573 1342 7536 Fragments arranged by size: 7536 1342 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6901 7662 8690 1540 Size: 275 186 4900 761 1028 2301 Fragments arranged by size: 4900 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 6231 7512 7597 8668 2217 Size: 4014 1281 85 1071 3000 Fragments arranged by size: 4014 3000 1281 1071 85 BsmI GAATG_Cn' Cuts at: 3796 6962 7055 3796 Size: 3166 93 6192 Fragments arranged by size: 6192 3166 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2615 3468 2615 Size: 853 8598 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2217 2230 2519 3399 3818 3884 Size: 399 283 144 13 289 880 419 66 Cuts at: 3884 5622 6231 6658 6770 7512 7597 8668 1391 Size: 1738 609 427 112 742 85 1071 2174 Fragments arranged by size: 2174 1738 1071 880 742 609 427 419 399 289 283 144 112 85 66 13 BspEI T'CCGG_A Cuts at: 3786 9114 3786 Size: 5328 4123 BspHI T'CATG_A Cuts at: 2872 4191 4371 7340 9070 2872 Size: 1319 180 2969 1730 3253 Fragments arranged by size: 3253 2969 1730 1319 180 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4915 5681 6806 844 Size: 4071 766 1125 3489 Fragments arranged by size: 4071 3489 1125 766 BsrBI CCG'CTC Cuts at: 1394 1823 7338 1394 Size: 429 5515 3507 Fragments arranged by size: 5515 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2700 2751 3739 7946 8120 65 Size: 1183 1452 51 988 4207 174 1396 Fragments arranged by size: 4207 1452 1396 1183 988 174 51 BsrFI r'CCGG_y Cuts at: 1700 1810 4669 8095 8282 1700 Size: 110 2859 3426 187 2869 Fragments arranged by size: 3426 2869 2859 187 110 BsrGI T'GTAC_A Cuts at: 96 4211 96 Size: 4115 5336 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9451 BssSI C'ACGA_G Cuts at: 5526 7511 8523 5526 Size: 1985 1012 6454 Fragments arranged by size: 6454 1985 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2777 3442 794 Size: 1983 665 6803 Fragments arranged by size: 6803 1983 665 BstBI TT'CG_AA Cuts at: 2440 2896 4132 8255 2440 Size: 456 1236 4123 3636 Fragments arranged by size: 4123 3636 1236 456 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9451 BstXI CCAn_nnnn'nTGG Cuts at: 4120 6061 4120 Size: 1941 7510 BstYI r'GATC_y Cuts at: 1972 2137 2531 2556 2689 2761 3300 4073 4435 Size: 165 394 25 133 72 539 773 362 Cuts at: 4435 5928 6781 7148 7533 7550 9002 9088 9100 Size: 1493 853 367 385 17 1452 86 12 Cuts at: 9100 9446 1972 Size: 346 1977 Fragments arranged by size: 1977 1493 1452 853 773 539 394 385 367 362 346 165 133 86 72 25 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 4113 5509 6666 513 Size: 552 939 2109 1396 1157 3298 Fragments arranged by size: 3298 2109 1396 1157 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4820 487 Size: 683 56 3594 5118 Fragments arranged by size: 5118 3594 683 56 BtsI GCAGTG_nn' Cuts at: 2050 3884 6121 6181 6967 7761 7781 2050 Size: 1834 2237 60 786 794 20 3720 Fragments arranged by size: 3720 2237 1834 794 786 60 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9451 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6136 6171 529 Size: 35 5572 35 3809 Fragments arranged by size: 5572 3809 35 35 DraI TTT'AAA Cuts at: 4864 6755 4864 Size: 1891 7560 DraIII CAC_nnn'GTG Cuts at: 6341 6341 Size: 9451 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2937 4313 8458 809 Size: 507 1621 1376 4145 1802 Fragments arranged by size: 4145 1802 1621 1376 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2748 4146 6051 Size: 54 1425 50 275 18 918 1398 1905 Cuts at: 6051 6898 7787 9416 8 Size: 847 889 1629 43 Fragments arranged by size: 1905 1629 1425 1398 918 889 847 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6898 1537 Size: 275 5086 4090 Fragments arranged by size: 5086 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2017 2222 2310 2964 3099 3511 3747 3929 Size: 664 205 88 654 135 412 236 182 Cuts at: 3929 5822 5979 7381 1353 Size: 1893 157 1402 3423 Fragments arranged by size: 3423 1893 1402 664 654 412 236 205 182 157 135 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8038 8412 8558 387 Size: 1064 138 6449 374 146 1280 Fragments arranged by size: 6449 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2215 6229 2215 Size: 4014 5437 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2523 2547 2582 3335 3492 3866 4313 Size: 393 891 24 35 753 157 374 447 Cuts at: 4313 4592 5388 5498 5860 6023 6315 6366 7514 Size: 279 796 110 362 163 292 51 1148 Cuts at: 7514 8111 8898 9166 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1148 891 796 787 753 597 447 393 374 362 292 279 268 163 157 110 51 35 24 EcoNI CCTnn'n_nnAGG Cuts at: 6321 6321 Size: 9451 EcoO109I rG'GnC_Cy Cuts at: 2939 6377 6654 2939 Size: 3438 277 5736 Fragments arranged by size: 5736 3438 277 EcoRI G'AATT_C Cuts at: 6760 7138 6760 Size: 378 9073 EcoRV GAT'ATC Cuts at: 3481 3481 Size: 9451 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2978 3150 4268 4289 5149 2978 Size: 172 1118 21 860 7280 Fragments arranged by size: 7280 1118 860 172 21 FspI TGC'GCA Cuts at: 7957 7957 Size: 9451 HaeII r_GCGC'y Cuts at: 1678 6688 8598 1678 Size: 5010 1910 2531 Fragments arranged by size: 5010 2531 1910 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3098 3130 3465 3497 4154 Size: 32 135 32 1526 32 335 32 657 Cuts at: 4154 4186 4214 4246 4292 4324 4878 4910 5714 Size: 32 28 32 46 32 554 32 804 Cuts at: 5714 5746 6420 6452 6579 6611 8092 8124 8166 Size: 32 674 32 127 32 1481 32 42 Cuts at: 8166 8198 1373 Size: 32 2626 Fragments arranged by size: 2626 1526 1481 804 674 657 554 335 135 127 46 42 32 32 32 32 32 32 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 2085 6795 7041 669 Size: 988 281 147 4710 246 3079 Fragments arranged by size: 4710 3079 988 281 246 147 HindIII A'AGCT_T Cuts at: 748 748 Size: 9451 HpaI GTT'AAC Cuts at: 7041 7041 Size: 9451 KasI G'GCGC_C Cuts at: 6684 6684 Size: 9451 KpnI G_GTAC'C Cuts at: 6776 6776 Size: 9451 MfeI C'AATT_G Cuts at: 5886 7050 5886 Size: 1164 8287 MluI A'CGCG_T Cuts at: 9327 9327 Size: 9451 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2153 2320 2937 3173 4864 6570 8565 8749 Size: 848 167 617 236 1691 1706 1995 184 Cuts at: 8749 1305 Size: 2007 Fragments arranged by size: 2007 1995 1706 1691 848 617 236 184 167 MscI TGG'CCA Cuts at: 10 64 1489 1832 2750 4148 6053 10 Size: 54 1425 343 918 1398 1905 3408 Fragments arranged by size: 3408 1905 1425 1398 918 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3804 4118 4383 4704 5463 5627 6665 Size: 648 2638 314 265 321 759 164 1038 Cuts at: 6665 7409 7768 7927 518 Size: 744 359 159 2042 Fragments arranged by size: 2638 2042 1038 759 744 648 359 321 314 265 164 159 MspA1I CmG'CkG Cuts at: 1606 2052 4967 6425 6490 6867 7544 8692 8937 Size: 446 2915 1458 65 377 677 1148 245 Cuts at: 8937 1606 Size: 2120 Fragments arranged by size: 2915 2120 1458 1148 677 446 377 245 65 NarI GG'CG_CC Cuts at: 6685 6685 Size: 9451 NcoI C'CATG_G Cuts at: 513 1065 2004 4113 5509 6666 513 Size: 552 939 2109 1396 1157 3298 Fragments arranged by size: 3298 2109 1396 1157 939 552 NdeI CA'TA_TG Cuts at: 387 4533 6352 387 Size: 4146 1819 3486 Fragments arranged by size: 4146 3486 1819 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9451 NotI GC'GGCC_GC Cuts at: 6898 6898 Size: 9451 NspI r_CATG'y Cuts at: 1132 1229 4876 5473 5688 6809 8354 1132 Size: 97 3647 597 215 1121 1545 2229 Fragments arranged by size: 3647 2229 1545 1121 597 215 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9451 PciI A'CATG_T Cuts at: 5469 5684 8350 5469 Size: 215 2666 6570 Fragments arranged by size: 6570 2666 215 PflMI CCAn_nnn'nTGG Cuts at: 1108 5515 6310 1108 Size: 4407 795 4249 Fragments arranged by size: 4407 4249 795 PfoI T'CCnGG_A Cuts at: 2559 3843 6705 2559 Size: 1284 2862 5305 Fragments arranged by size: 5305 2862 1284 PmeI GTTT'AAAC Cuts at: 6755 6755 Size: 9451 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7520 7552 9033 9065 7520 Size: 32 1481 32 7906 Fragments arranged by size: 7906 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2939 2939 Size: 9451 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9451 PsiI TTA'TAA Cuts at: 2385 2790 3681 3828 7021 2385 Size: 405 891 147 3193 4815 Fragments arranged by size: 4815 3193 891 405 147 PspOMI G'GGCC_C Cuts at: 3880 6654 3880 Size: 2774 6677 PstI C_TGCA'G Cuts at: 830 2926 4819 4912 5738 6803 830 Size: 2096 1893 93 826 1065 3478 Fragments arranged by size: 3478 2096 1893 1065 826 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9451 PvuII CAG'CTG Cuts at: 4967 6425 4967 Size: 1458 7993 SacI G_AGCT'C Cuts at: 2217 6231 2217 Size: 4014 5437 SalI G'TCGA_C Cuts at: 1936 6793 1936 Size: 4857 4594 SapI GCTCTTCn'nnn_ Cuts at: 2017 2222 2017 Size: 205 9246 SbfI CC_TGCA'GG Cuts at: 5738 6803 5738 Size: 1065 8386 ScaI AGT'ACT Cuts at: 7699 9411 7699 Size: 1712 7739 SexAI A'CCwGG_T Cuts at: 5431 6337 5431 Size: 906 8545 SfcI C'TryA_G Cuts at: 826 1045 2133 2685 2922 4402 4815 4855 4908 Size: 219 1088 552 237 1480 413 40 53 Cuts at: 4908 5734 6799 7934 8615 8806 826 Size: 826 1065 1135 681 191 1471 Fragments arranged by size: 1480 1471 1135 1088 1065 826 681 552 413 237 219 191 53 40 SfoI GGC'GCC Cuts at: 6686 6686 Size: 9451 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9451 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9451 SmaI CCC'GGG Cuts at: 1872 6778 1872 Size: 4906 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2693 2805 3603 3857 3920 6263 Size: 197 926 750 112 798 254 63 2343 Cuts at: 6263 7555 8456 8718 820 Size: 1292 901 262 1553 Fragments arranged by size: 2343 1553 1292 926 901 798 750 262 254 197 112 63 SnaBI TAC'GTA Cuts at: 493 7242 493 Size: 6749 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9451 SphI G_CATG'C Cuts at: 1132 6809 1132 Size: 5677 3774 SspI AAT'ATT Cuts at: 5 52 2841 7375 5 Size: 47 2789 4534 2081 Fragments arranged by size: 4534 2789 2081 47 StuI AGG'CCT Cuts at: 1517 4821 6509 1517 Size: 3304 1688 4459 Fragments arranged by size: 4459 3304 1688 StyI C'CwwG_G Cuts at: 513 1065 2004 2962 3202 4113 5509 6304 6666 Size: 552 939 958 240 911 1396 795 362 Cuts at: 6666 6890 513 Size: 224 3074 Fragments arranged by size: 3074 1396 958 939 911 795 552 362 240 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5613 6222 6472 7478 7495 7648 7833 Size: 1255 4033 609 250 1006 17 153 185 Cuts at: 7833 8278 325 Size: 445 1498 Fragments arranged by size: 4033 1498 1255 1006 609 445 250 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3013 4211 7697 Size: 274 80 33 51 1443 1036 1198 3486 Cuts at: 7697 9409 96 Size: 1712 138 Fragments arranged by size: 3486 1712 1443 1198 1036 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6348 6380 9411 9443 6348 Size: 32 3031 32 6356 Fragments arranged by size: 6356 3031 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4187 5492 1316 Size: 2871 1305 5275 Fragments arranged by size: 5275 2871 1305 XbaI T'CTAG_A Cuts at: 4070 5041 6787 4070 Size: 971 1746 6734 Fragments arranged by size: 6734 1746 971 XhoI C'TCGA_G Cuts at: 1943 3603 1943 Size: 1660 7791 XmaI C'CCGG_G Cuts at: 1870 6776 1870 Size: 4906 4545 XmnI GAAnn'nnTTC Cuts at: 2625 4128 5649 5813 7580 2625 Size: 1503 1521 164 1767 4496 Fragments arranged by size: 4496 1767 1521 1503 164 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2609 6595 7259 276 Size: 53 83 186 936 1075 3986 664 2468 Fragments arranged by size: 3986 2468 1075 936 664 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI XcmI