################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AlwNI Aor51HI AscI Asp718I AvaII AviII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcsI 1248 AAATTC [GA]^AATT[CT] Alw44I 151 GTGCAC G^TGCAC ApaI 1417 GGGCCC GGGCC^C ApaLI 151 GTGCAC G^TGCAC ApoI 1248 AAATTC [GA]^AATT[CT] Asp700I 18 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 151 GTGCAC G[AT]GC[AT]^C AspI 1492 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1539 CCTAGG C^CTAGG BanI 1127 GGTGCC G^G[CT][GA]CC BglII 461 AGATCT A^GATCT BlnI 1539 CCTAGG C^CTAGG BlpI 41 GCTCAGC GC^T[ACGT]AGC Bpu1102I 41 GCTCAGC GC^T[ACGT]AGC BsiHKAI 151 GTGCAC G[AT]GC[AT]^C BsmAI 947 GAGAC GTCTC(1/5) BspHI 365 TCATGA T^CATGA BspMI 920 ACCTGC ACCTGC(4/8) BsrBI 721 GAGCGG CCGCTC(-3/-3) BsrFI 136 ACCGGC [GA]^CCGG[CT] BstXI 905 CCAGTGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 461 AGATCT [GA]^GATC[CT] CelII 41 GCTCAGC GC^T[ACGT]AGC CfoI 1555 GCGC GCG^C Cfr10I 136 ACCGGC [GA]^CCGG[CT] DraII 1271 AGGGCCT [GA]G^G[ACGT]CC[CT] EarI 1456 GAAGAG CTCTTC(1/4) EcoO109I 1271 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT22I 425 ATGCAT ATGCA^T FauI 66 GCGGG CCCGC(4/6) HhaI 1555 GCGC GCG^C HinP1I 1555 GCGC G^CGC HphI 208 GGTGA GGTGA(8/7) Ksp632I 1456 GAAGAG CTCTTC(1/4) MaeIII 1504 GTCAC ^GT[ACGT]AC MflI 461 AGATCT [GA]^GATC[CT] MslI 254 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 146 CCATGG C^CATGG NsiI 425 ATGCAT ATGCA^T PacI 1354 TTAATTAA TTAAT^TAA PflFI 1492 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 1279 CTGCAG CTGCA^G RcaI 365 TCATGA T^CATGA SapI 1456 GAAGAGC GCTCTTC(1/4) SfcI 1279 CTGCAG C^T[GA][CT]AG SphI 427 GCATGC GCATG^C TaqI 179 TCGA T^CGA Tsp45I 1504 GTCAC ^GT[GC]AC Tth111I 1492 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 179 TCGA T^CGA XcmI 331 CCAAGAACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 461 AGATCT [GA]^GATC[CT] XmnI 18 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AhdI 175 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 986 GACACATTGTC AseI 820 ATTAAT AT^TAAT 1375 ATTAAT AsnI 820 ATTAAT AT^TAAT 1375 ATTAAT AspEI 175 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 986 GACACATTGTC AvaI 1303 CTCGGG C^[CT]CG[GA]G 1444 CCCGAG BbsI 183 GTCTTC GAAGAC(2/6) 1342 GTCTTC BpuAI 183 GTCTTC GAAGAC(2/6) 1342 GTCTTC BsmI 492 GAATGC GAATGC(1/-1) 924 GCATTC BsoBI 1303 CTCGGG C^[CT]CG[GA]G 1444 CCCGAG Eam1105I 175 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 986 GACACATTGTC EcoNI 1263 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1278 CCTGCAGAAGG HapII 137 CCGG C^CGG 1066 CCGG HgaI 1496 GCGTC GACGC(5/10) 1514 GCGTC HpaII 137 CCGG C^CGG 1066 CCGG MspA1I 1190 CAGCTG C[AC]G^C[GT]G 1228 CAGCTG MspI 137 CCGG C^CGG 1066 CCGG PshBI 820 ATTAAT AT^TAAT 1375 ATTAAT PvuII 1190 CAGCTG CAG^CTG 1228 CAGCTG SfaNI 1152 GATGC GCATC(5/9) 111 GCATC SspI 964 AATATT AAT^ATT 1240 AATATT TfiI 23 GATTC G^A[AT]TC 976 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AfaI AluI AlwI AocI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI DpnII DsaI Eco81I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MaeI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI StyI Tru9I TseI Tsp509I TspRI