(Circular) (Six-base) MAPSORT of: pFN21AB0483.seq Check: 3167 from: 1 to: 6283 pFN21AB0483 6283 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4093 278 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3626 1937 Size: 1689 4594 Acc65I G'GTAC_C Cuts at: 3604 3604 Size: 6283 AclI AA'CG_TT Cuts at: 1563 4410 4783 1563 Size: 2847 373 3063 Fragments arranged by size: 3063 2847 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2153 2466 4346 5730 5998 1632 Size: 521 313 1880 1384 268 1917 Fragments arranged by size: 1917 1880 1384 521 313 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6086 AflIII A'CryG_T Cuts at: 1203 5182 6159 1203 Size: 3979 977 1327 Fragments arranged by size: 3979 1327 977 AgeI A'CCGG_T Cuts at: 5114 5114 Size: 6283 AhdI GACnn_n'nnGTC Cuts at: 2185 2996 5012 2185 Size: 811 2016 3456 Fragments arranged by size: 3456 2016 811 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6283 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6283 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2208 2240 2853 2885 2208 Size: 32 613 32 5606 Fragments arranged by size: 5606 613 32 32 AlwNI CAG_nnn'CTG Cuts at: 4026 5598 4026 Size: 1572 4711 ApaI G_GGCC'C Cuts at: 3426 3426 Size: 6283 ApaLI G'TGCA_C Cuts at: 2156 4340 5496 2156 Size: 2184 1156 2943 Fragments arranged by size: 2943 2184 1156 ApoI r'AATT_y Cuts at: 1493 3253 3592 3818 3970 6079 1493 Size: 1760 339 226 152 2109 1697 Fragments arranged by size: 2109 1760 1697 339 226 152 AseI AT'TA_AT Cuts at: 160 1032 2826 3381 4837 160 Size: 872 1794 555 1456 1606 Fragments arranged by size: 1794 1606 1456 872 555 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 AvaI C'yCGr_G Cuts at: 1870 1943 3308 3449 3608 1870 Size: 73 1365 141 159 4545 Fragments arranged by size: 4545 1365 159 141 73 AvrII C'CTAG_G Cuts at: 3544 3544 Size: 6283 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2066 2099 2066 Size: 33 6250 BamHI G'GATC_C Cuts at: 3613 3980 3613 Size: 367 5916 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3132 3604 5059 618 Size: 325 217 627 1345 472 1455 1842 Fragments arranged by size: 1842 1455 1345 627 472 325 217 BanII G_rGCy'C Cuts at: 1391 2096 2887 3426 3602 1391 Size: 705 791 539 176 4072 Fragments arranged by size: 4072 791 705 539 176 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2181 3340 928 Size: 971 282 1159 3871 Fragments arranged by size: 3871 1159 971 282 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4474 4508 1903 Size: 34 2537 34 3678 Fragments arranged by size: 3678 2537 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4175 5385 4175 Size: 1210 5073 BclI T'GATC_A Cuts at: 1548 1548 Size: 6283 BfrBI ATG'CAT Cuts at: 2432 2432 Size: 6283 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4894 136 Size: 107 122 71 631 605 3222 1525 Fragments arranged by size: 3222 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2466 6278 2466 Size: 3812 2471 BlpI GC'TnA_GC Cuts at: 2047 3700 2047 Size: 1653 4630 Bme1580I G_kGCm'C Cuts at: 1790 2160 3426 4344 5500 1790 Size: 370 1266 918 1156 2573 Fragments arranged by size: 2573 1266 1156 918 370 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6283 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2027 3376 4014 Size: 416 53 83 399 24 610 1349 638 Cuts at: 4014 4972 442 Size: 958 1753 Fragments arranged by size: 1753 1349 958 638 610 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6283 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2255 2255 Size: 6283 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2151 2837 3215 3293 4943 1239 Size: 912 686 378 78 1650 2579 Fragments arranged by size: 2579 1650 912 686 378 78 Bpu10I CC'TnA_GC Cuts at: 2487 2487 Size: 6283 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4408 5273 5571 4408 Size: 865 298 5120 Fragments arranged by size: 5120 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4946 882 Size: 617 3447 2219 Fragments arranged by size: 3447 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4074 493 Size: 711 2870 2702 Fragments arranged by size: 2870 2702 711 BsaBI GATnn'nnATC Cuts at: 6277 6277 Size: 6283 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4090 4472 5107 275 Size: 53 83 186 936 2557 382 635 1451 Fragments arranged by size: 2557 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4716 5114 5388 5535 5946 4716 Size: 398 274 147 411 5053 Fragments arranged by size: 5053 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2129 2159 2240 2270 2374 Size: 30 165 30 726 30 81 30 104 Cuts at: 2374 2404 1178 Size: 30 5057 Fragments arranged by size: 5057 726 165 104 81 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2137 2395 2502 2720 1186 Size: 951 258 107 218 4749 Fragments arranged by size: 4749 951 258 218 107 BseYI C'CCAG_C Cuts at: 2126 5486 5982 2126 Size: 3360 496 2427 Fragments arranged by size: 3360 2427 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5710 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3733 4494 5522 1540 Size: 275 186 1732 761 1028 2301 Fragments arranged by size: 2301 1732 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2160 4344 4429 5500 2160 Size: 2184 85 1071 2943 Fragments arranged by size: 2943 2184 1071 85 BsmI GAATG_Cn' Cuts at: 2503 2929 3794 3887 2503 Size: 426 865 93 4899 Fragments arranged by size: 4899 865 426 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2160 2887 3426 3602 4344 4429 Size: 399 306 64 727 539 176 742 85 Cuts at: 4429 5500 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 727 539 399 306 176 85 64 BspEI T'CCGG_A Cuts at: 5946 5946 Size: 6283 BspHI T'CATG_A Cuts at: 2370 4172 5902 2370 Size: 1802 1730 2751 Fragments arranged by size: 2751 1802 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2934 3638 844 Size: 2090 704 3489 Fragments arranged by size: 3489 2090 704 BsrBI CCG'CTC Cuts at: 1394 1823 2728 4170 1394 Size: 429 905 1442 3507 Fragments arranged by size: 3507 1442 905 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4778 4952 65 Size: 1183 3530 174 1396 Fragments arranged by size: 3530 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2141 4927 5114 1700 Size: 110 331 2786 187 2869 Fragments arranged by size: 2869 2786 331 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6283 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6283 BssSI C'ACGA_G Cuts at: 4343 5355 4343 Size: 1012 5271 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6283 BstBI TT'CG_AA Cuts at: 5087 5087 Size: 6283 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6283 BstXI CCAn_nnnn'nTGG Cuts at: 2917 2917 Size: 6283 BstYI r'GATC_y Cuts at: 1972 2466 3613 3980 4365 4382 5834 5920 5932 Size: 494 1147 367 385 17 1452 86 12 Cuts at: 5932 6278 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1147 494 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2244 2478 3087 2244 Size: 234 609 5440 Fragments arranged by size: 5440 609 234 BtgI C'CryG_G Cuts at: 513 1065 2004 2151 2879 3567 513 Size: 552 939 147 728 688 3229 Fragments arranged by size: 3229 939 728 688 552 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5544 Fragments arranged by size: 5544 683 56 BtsI GCAGTG_nn' Cuts at: 3799 4593 4613 3799 Size: 794 20 5469 Fragments arranged by size: 5469 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6283 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6248 DraI TTT'AAA Cuts at: 3587 3587 Size: 6283 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5290 809 Size: 507 3974 1802 Fragments arranged by size: 3974 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3730 4619 6248 Size: 54 1425 50 275 18 1900 889 1629 Cuts at: 6248 8 Size: 43 Fragments arranged by size: 1900 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3730 1537 Size: 275 1918 4090 Fragments arranged by size: 4090 1918 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3456 4213 1353 Size: 2103 757 3423 Fragments arranged by size: 3423 2103 757 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4870 5244 5390 387 Size: 1064 138 3281 374 146 1280 Fragments arranged by size: 3281 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 2153 2466 2837 3215 3293 4346 Size: 393 519 2 313 371 378 78 1053 Cuts at: 4346 4943 5730 5998 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1053 787 597 519 393 378 371 313 268 78 2 EcoNI CCTnn'n_nnAGG Cuts at: 3272 3287 3272 Size: 15 6268 EcoO109I rG'GnC_Cy Cuts at: 3277 3277 Size: 6283 EcoRI G'AATT_C Cuts at: 3592 3970 3592 Size: 378 5905 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3340 3340 Size: 6283 FspI TGC'GCA Cuts at: 4789 4789 Size: 6283 HaeII r_GCGC'y Cuts at: 1678 5430 1678 Size: 3752 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2019 2051 2171 2203 2240 Size: 32 135 32 447 32 120 32 37 Cuts at: 2240 2272 2374 2406 2982 3014 3325 3357 4924 Size: 32 102 32 576 32 311 32 1567 Cuts at: 4924 4956 4998 5030 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1567 576 447 311 135 120 102 42 37 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3627 3873 669 Size: 988 281 1689 246 3079 Fragments arranged by size: 3079 1689 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6283 HpaI GTT'AAC Cuts at: 3873 3873 Size: 6283 KpnI G_GTAC'C Cuts at: 3608 3608 Size: 6283 MfeI C'AATT_G Cuts at: 3882 3882 Size: 6283 MluI A'CGCG_T Cuts at: 6159 6159 Size: 6283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2703 5397 5581 1305 Size: 1398 2694 184 2007 Fragments arranged by size: 2694 2007 1398 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4461 Fragments arranged by size: 4461 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2263 4241 4600 4759 518 Size: 648 1097 1978 359 159 2042 Fragments arranged by size: 2042 1978 1097 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3197 3235 3699 4376 5524 5769 1606 Size: 1591 38 464 677 1148 245 2120 Fragments arranged by size: 2120 1591 1148 677 464 245 38 NcoI C'CATG_G Cuts at: 513 1065 2004 2151 513 Size: 552 939 147 4645 Fragments arranged by size: 4645 939 552 147 NdeI CA'TA_TG Cuts at: 387 387 Size: 6283 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6283 NotI GC'GGCC_GC Cuts at: 3730 3730 Size: 6283 NsiI A_TGCA'T Cuts at: 2434 2434 Size: 6283 NspI r_CATG'y Cuts at: 1132 1229 2208 2222 2436 3641 5186 1132 Size: 97 979 14 214 1205 1545 2229 Fragments arranged by size: 2229 1545 1205 979 214 97 14 PacI TTA_AT'TAA Cuts at: 3363 3363 Size: 6283 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6283 PciI A'CATG_T Cuts at: 5182 5182 Size: 6283 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6283 PfoI T'CCnGG_A Cuts at: 2871 3270 2871 Size: 399 5884 PmeI GTTT'AAAC Cuts at: 3587 3587 Size: 6283 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4352 4384 5865 5897 4352 Size: 32 1481 32 4738 Fragments arranged by size: 4738 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6283 PsiI TTA'TAA Cuts at: 3853 3853 Size: 6283 PspOMI G'GGCC_C Cuts at: 3422 3422 Size: 6283 PstI C_TGCA'G Cuts at: 830 3288 3635 830 Size: 2458 347 3478 Fragments arranged by size: 3478 2458 347 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6283 PvuII CAG'CTG Cuts at: 3197 3235 3197 Size: 38 6245 SalI G'TCGA_C Cuts at: 1936 3625 1936 Size: 1689 4594 SapI GCTCTTCn'nnn_ Cuts at: 3456 3456 Size: 6283 SbfI CC_TGCA'GG Cuts at: 3635 3635 Size: 6283 ScaI AGT'ACT Cuts at: 4531 6243 4531 Size: 1712 4571 SfcI C'TryA_G Cuts at: 826 1045 3284 3631 4766 5447 5638 826 Size: 219 2239 347 1135 681 191 1471 Fragments arranged by size: 2239 1471 1135 681 347 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6283 SmaI CCC'GGG Cuts at: 1872 3610 1872 Size: 1738 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4387 5288 5550 820 Size: 197 926 2444 901 262 1553 Fragments arranged by size: 2444 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4074 493 Size: 3581 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6283 SphI G_CATG'C Cuts at: 1132 2436 3641 1132 Size: 1304 1205 3774 Fragments arranged by size: 3774 1304 1205 SspI AAT'ATT Cuts at: 5 52 2971 3247 4207 5 Size: 47 2919 276 960 2081 Fragments arranged by size: 2919 2081 960 276 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6283 StyI C'CwwG_G Cuts at: 513 1065 2004 2151 2542 2916 3315 3544 3722 Size: 552 939 147 391 374 399 229 178 Cuts at: 3722 513 Size: 3074 Fragments arranged by size: 3074 939 552 399 391 374 229 178 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2201 2271 4310 4327 4480 4665 5110 Size: 1255 621 70 2039 17 153 185 445 Cuts at: 5110 325 Size: 1498 Fragments arranged by size: 2039 1498 1255 621 445 185 153 70 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2136 2805 4529 Size: 274 80 33 51 1443 159 669 1724 Cuts at: 4529 6241 96 Size: 1712 138 Fragments arranged by size: 1724 1712 1443 669 274 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3116 3148 6243 6275 3116 Size: 32 3095 32 3124 Fragments arranged by size: 3124 3095 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3500 1316 Size: 2184 4099 XbaI T'CTAG_A Cuts at: 3619 3619 Size: 6283 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2343 2343 Size: 6283 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6283 XmaI C'CCGG_G Cuts at: 1870 3608 1870 Size: 1738 4545 XmnI GAAnn'nnTTC Cuts at: 2027 4412 2027 Size: 2385 3898 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4091 276 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BbeI BbvCI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI