################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AccIII AclI Alw44I ApaLI AscI Asp718I AviII BbeI BcgI BclI BglI BlpI Bpu1102I BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssHII CelII ClaI CpoI EagI EclXI Eco52I EcoT22I FauI FbaI FseI FspI KasI KpnI KspI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PmeI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SmaI SpeI SphI Sse8387I SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccII 5501 CGCG CG^CG AcyI 331 GACGCC G[GA]^CG[CT]C AgeI 3891 ACCGGT A^CCGGT AhdI 5044 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 950 GGGCCC GGGCC^C AseI 1192 ATTAAT AT^TAAT AsnI 1192 ATTAAT AT^TAAT AspEI 5044 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 5760 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 5224 CCCGAG C^[CT]CG[GA]G AvrII 1120 CCTAGG C^CTAGG BanI 4101 GGTGCC G^G[CT][GA]CC BbvCI 13 GCTGAGG CCTCAGC(-5/-2) BcnI 5979 CCCGG CC^[GC]GG BglII 4616 AGATCT A^GATCT BlnI 1120 CCTAGG C^CTAGG BsaBI 2172 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 331 GACGCC G[GA]^CG[CT]C BsaWI 3891 ACCGGT [AT]^CCGG[AT] BseRI 2365 GAGGAG GAGGAG(10/8) BsoBI 5224 CCCGAG C^[CT]CG[GA]G BspHI 2807 TCATGA T^CATGA BsrFI 3891 ACCGGT [GA]^CCGG[CT] Bst1107I 4758 GTATAC GTA^TAC BstAPI 5851 GCACTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3238 TTCGAA TT^CGAA BstUI 5501 CGCG CG^CG BstZ17I 4758 GTATAC GTA^TAC Cfr10I 3891 ACCGGT [GA]^CCGG[CT] DrdI 6006 GACAGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1968 CCATGG C^C[GA][CT]GG Eam1105I 5044 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3835 CCTAAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 3979 GATATC GAT^ATC HgaI 331 GACGC GACGC(5/10) Hin1I 331 GACGCC G[GA]^CG[CT]C HincII 1836 GTTAAC GT[CT]^[GA]AC HindII 1836 GTTAAC GT[CT]^[GA]AC HindIII 1473 AAGCTT A^AGCTT HpaI 1836 GTTAAC GTT^AAC MamI 2172 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 5501 CGCG CG^CG NciI 5979 CCCGG CC^[GC]GG NcoI 1968 CCATGG C^CATGG NspV 3238 TTCGAA TT^CGAA PflFI 5760 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 5380 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 3891 ACCGGT A^CCGGT PshBI 1192 ATTAAT AT^TAAT RcaI 2807 TCATGA T^CATGA SacI 812 GAGCTC GAGCT^C ScaI 2042 AGTACT AGT^ACT SfuI 3238 TTCGAA TT^CGAA SnaBI 3954 TACGTA TAC^GTA SspI 2661 AATATT AAT^ATT StuI 1532 AGGCCT AGG^CCT Tth111I 5760 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 5380 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 4764 CCAGAAAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 2419 GTAGAC GT^[AC][GT]AC 4758 GTATAC AflII 2373 CTTAAG C^TTAAG 4552 CTTAAG AflIII 4010 ACGTGT A^C[GA][CT]GT 4377 ACACGT AocI 997 CCTGAGG CC^T[ACGT]AGG 1654 CCTGAGG Aor51HI 1522 AGCGCT AGC^GCT 5971 AGCGCT BfrI 2373 CTTAAG C^TTAAG 4552 CTTAAG BsaI 4567 GAGACC GGTCTC(1/5) 3819 GGTCTC BsmI 1918 GAATGC GAATGC(1/-1) 4170 GAATGC BssSI 1848 CACGAG CACGAG(-5/-1) 5881 CACGAG BstEII 805 GGTAACC G^GT[ACGT]ACC 4189 GGTCACC BstPI 805 GGTAACC G^GT[ACGT]ACC 4189 GGTCACC BstXI 3913 CCAACTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4571 CCAGGGACCTGG Bsu36I 997 CCTGAGG CC^T[ACGT]AGG 1654 CCTGAGG DraI 3453 TTTAAA TTT^AAA 3640 TTTAAA Eco47III 1522 AGCGCT AGC^GCT 5971 AGCGCT Eco81I 997 CCTGAGG CC^T[ACGT]AGG 1654 CCTGAGG EcoO65I 805 GGTAACC G^GT[ACGT]ACC 4189 GGTCACC HaeII 1522 AGCGCT [GA]GCGC^[CT] 5971 AGCGCT NspI 1026 GCATGT [GA]CATG^[CT] 2670 GCATGT PpuMI 4574 GGGACCT [GA]G^G[AT]CC[CT] 5764 AGGTCCT SapI 2401 GAAGAGC GCTCTTC(1/4) 6065 GCTCTTC SexAI 4017 ACCAGGT A^CC[AT]GGT 5761 ACCAGGT ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BamHI BanII BbrPI BbsI BbvI BciVI BfaI BmyI BpmI BpuAI BsaAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NdeII NlaIII NlaIV PleI PmaCI PmlI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI