(Circular) (Six-base) MAPSORT of: pFN21AE3713.seq Check: 4564 from: 1 to: 10780 pFN21AE3713 10780 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8590 278 Size: 53 83 186 936 7054 2468 Fragments arranged by size: 7054 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4425 6764 8123 1937 Size: 2488 2339 1359 4594 Fragments arranged by size: 4594 2488 2339 1359 Acc65I G'GTAC_C Cuts at: 8101 8101 Size: 10780 AclI AA'CG_TT Cuts at: 1563 8907 9280 1563 Size: 7344 373 3063 Fragments arranged by size: 7344 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2906 3693 4171 5112 5537 5670 6743 7025 Size: 1274 787 478 941 425 133 1073 282 Cuts at: 7025 7233 7422 8843 10227 10495 1632 Size: 208 189 1421 1384 268 1917 Fragments arranged by size: 1917 1421 1384 1274 1073 941 787 478 425 282 268 208 189 133 AfeI AGC'GCT Cuts at: 3529 7978 3529 Size: 4449 6331 AflII C'TTAA_G Cuts at: 820 1017 4378 6557 820 Size: 197 3361 2179 5043 Fragments arranged by size: 5043 3361 2179 197 AflIII A'CryG_T Cuts at: 1203 6015 6382 9679 10656 1203 Size: 4812 367 3297 977 1327 Fragments arranged by size: 4812 3297 1327 977 367 AgeI A'CCGG_T Cuts at: 5896 9611 5896 Size: 3715 7065 AhdI GACnn_n'nnGTC Cuts at: 7054 9509 7054 Size: 2455 8325 AleI CACnn'nnGTG Cuts at: 1166 7760 1166 Size: 6594 4186 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6411 1184 Size: 5227 5553 AlwNI CAG_nnn'CTG Cuts at: 5004 5192 5213 5852 8059 8523 10095 5004 Size: 188 21 639 2207 464 1572 5689 Fragments arranged by size: 5689 2207 1572 639 464 188 21 ApaI G_GGCC'C Cuts at: 2959 2959 Size: 10780 ApaLI G'TGCA_C Cuts at: 8837 9993 8837 Size: 1156 9624 ApoI r'AATT_y Cuts at: 1493 2096 2185 2192 2365 3284 3911 4061 4214 Size: 603 89 7 173 919 627 150 153 Cuts at: 4214 4461 4594 4979 5182 5240 5583 5642 5731 Size: 247 133 385 203 58 343 59 89 Cuts at: 5731 6322 6477 8089 8315 8467 10576 1493 Size: 591 155 1612 226 152 2109 1697 Fragments arranged by size: 2109 1697 1612 919 627 603 591 385 343 247 226 203 173 155 153 152 150 133 89 89 59 58 7 AseI AT'TA_AT Cuts at: 160 1032 3198 9334 160 Size: 872 2166 6136 1606 Fragments arranged by size: 6136 2166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10780 AvaI C'yCGr_G Cuts at: 1870 1943 7229 8105 1870 Size: 73 5286 876 4545 Fragments arranged by size: 5286 4545 876 73 AvrII C'CTAG_G Cuts at: 3125 3125 Size: 10780 BamHI G'GATC_C Cuts at: 3085 4722 7321 8110 8477 3085 Size: 1637 2599 789 367 5388 Fragments arranged by size: 5388 2599 1637 789 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6106 8101 9556 618 Size: 325 217 627 4319 1995 1455 1842 Fragments arranged by size: 4319 1995 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2821 2959 4707 5932 6233 7746 7784 8099 Size: 1430 138 1748 1225 301 1513 38 315 Cuts at: 8099 1391 Size: 4072 Fragments arranged by size: 4072 1748 1513 1430 1225 315 301 138 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2643 3063 3821 4311 4572 5172 7239 Size: 971 744 420 758 490 261 600 2067 Cuts at: 7239 928 Size: 4469 Fragments arranged by size: 4469 2067 971 758 744 600 490 420 261 BbvCI CC'TCA_GC Cuts at: 2019 2019 Size: 10780 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8971 9005 1903 Size: 34 7034 34 3678 Fragments arranged by size: 7034 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5785 5945 6940 8672 9882 5785 Size: 160 995 1732 1210 6683 Fragments arranged by size: 6683 1732 1210 995 160 BclI T'GATC_A Cuts at: 1548 1548 Size: 10780 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9391 136 Size: 107 122 71 631 605 7719 1525 Fragments arranged by size: 7719 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6621 10775 6621 Size: 4154 6626 BlpI GC'TnA_GC Cuts at: 8197 8197 Size: 10780 Bme1580I G_kGCm'C Cuts at: 1790 2959 7932 8841 9997 1790 Size: 1169 4973 909 1156 2573 Fragments arranged by size: 4973 2573 1169 1156 909 BmgBI CAC'GTC Cuts at: 1318 7291 7499 1318 Size: 5973 208 4599 Fragments arranged by size: 5973 4599 208 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2962 3708 6028 Size: 416 53 83 399 24 1545 746 2320 Cuts at: 6028 7868 8037 8511 9469 442 Size: 1840 169 474 958 1753 Fragments arranged by size: 2320 1840 1753 1545 958 746 474 416 399 169 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10780 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7765 7765 Size: 10780 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3855 3921 7476 9440 1239 Size: 2616 66 3555 1964 2579 Fragments arranged by size: 3555 2616 2579 1964 66 Bpu10I CC'TnA_GC Cuts at: 2019 2019 Size: 10780 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2408 5522 5924 6245 8905 9770 10068 2408 Size: 3114 402 321 2660 865 298 3120 Fragments arranged by size: 3120 3114 2660 865 402 321 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5830 6566 9443 882 Size: 617 4331 736 2877 2219 Fragments arranged by size: 4331 2877 2219 736 617 BsaAI yAC'GTr Cuts at: 493 1204 2071 2351 5961 6385 8571 493 Size: 711 867 280 3610 424 2186 2702 Fragments arranged by size: 3610 2702 2186 867 711 424 280 BsaBI GATnn'nnATC Cuts at: 4181 10774 4181 Size: 6593 4187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2337 8587 8969 9604 Size: 53 83 186 936 804 6250 382 635 Cuts at: 9604 275 Size: 1451 Fragments arranged by size: 6250 1451 936 804 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5896 9213 9611 9885 10032 10443 5896 Size: 3317 398 274 147 411 6233 Fragments arranged by size: 6233 3317 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3401 3431 1178 Size: 30 165 30 1998 30 8527 Fragments arranged by size: 8527 1998 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4385 1186 Size: 3199 7581 BseYI C'CCAG_C Cuts at: 2938 4954 7012 7745 9983 10479 2938 Size: 2016 2058 733 2238 496 3239 Fragments arranged by size: 3239 2238 2058 2016 733 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3423 3516 6384 6407 7205 1163 Size: 573 1687 93 2868 23 798 4738 Fragments arranged by size: 4738 2868 1687 798 573 93 23 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8230 8991 10019 1540 Size: 275 186 6229 761 1028 2301 Fragments arranged by size: 6229 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2821 4562 6315 6501 7554 7920 8841 8926 9997 Size: 1741 1753 186 1053 366 921 85 1071 Cuts at: 9997 2821 Size: 3604 Fragments arranged by size: 3604 1753 1741 1071 1053 921 366 186 85 BsmI GAATG_Cn' Cuts at: 3929 6181 8291 8384 3929 Size: 2252 2110 93 6325 Fragments arranged by size: 6325 2252 2110 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2821 2959 4562 4707 5932 6233 6315 Size: 399 1031 138 1603 145 1225 301 82 Cuts at: 6315 6501 7554 7746 7784 7920 7932 8099 8841 Size: 186 1053 192 38 136 12 167 742 Cuts at: 8841 8926 9997 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1603 1225 1071 1053 1031 742 399 301 192 186 167 145 138 136 85 82 38 12 BspEI T'CCGG_A Cuts at: 10443 10443 Size: 10780 BspHI T'CATG_A Cuts at: 4812 8669 10399 4812 Size: 3857 1730 5193 Fragments arranged by size: 5193 3857 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 8135 844 Size: 7291 3489 BsrBI CCG'CTC Cuts at: 1394 1823 8667 1394 Size: 429 6844 3507 Fragments arranged by size: 6844 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2113 2502 4958 9275 9449 65 Size: 1183 865 389 2456 4317 174 1396 Fragments arranged by size: 4317 2456 1396 1183 865 389 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5896 9424 9611 1700 Size: 110 4086 3528 187 2869 Fragments arranged by size: 4086 3528 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10780 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10780 BssSI C'ACGA_G Cuts at: 3853 7886 8840 9852 3853 Size: 4033 954 1012 4781 Fragments arranged by size: 4781 4033 1012 954 BstAPI GCAn_nnn'nTGC Cuts at: 794 7862 794 Size: 7068 3712 BstBI TT'CG_AA Cuts at: 5244 9584 5244 Size: 4340 6440 BstEII G'GTnAC_C Cuts at: 1184 2810 6194 1184 Size: 1626 3384 5770 Fragments arranged by size: 5770 3384 1626 BstXI CCAn_nnnn'nTGG Cuts at: 5925 6583 5925 Size: 658 10122 BstYI r'GATC_y Cuts at: 1972 3016 3085 3565 3987 4326 4722 6219 6621 Size: 1044 69 480 422 339 396 1497 402 Cuts at: 6621 7321 8110 8477 8862 8879 10331 10417 10429 Size: 700 789 367 385 17 1452 86 12 Cuts at: 10429 10775 1972 Size: 346 1977 Fragments arranged by size: 1977 1497 1452 1044 789 700 480 422 402 396 385 367 346 339 86 69 17 12 BstZ17I GTA'TAC Cuts at: 6765 6765 Size: 10780 Bsu36I CC'TnA_GG Cuts at: 3003 3660 3003 Size: 657 10123 BtgI C'CryG_G Cuts at: 513 1065 2004 3973 513 Size: 552 939 1969 7320 Fragments arranged by size: 7320 1969 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2265 2547 487 Size: 683 56 1039 282 8720 Fragments arranged by size: 8720 1039 683 282 56 BtsI GCAGTG_nn' Cuts at: 4198 4617 5214 6256 7964 8296 9090 9110 4198 Size: 419 597 1042 1708 332 794 20 5868 Fragments arranged by size: 5868 1708 1042 794 597 419 332 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10780 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10745 DraI TTT'AAA Cuts at: 5460 5647 8084 5460 Size: 187 2437 8156 Fragments arranged by size: 8156 2437 187 DraIII CAC_nnn'GTG Cuts at: 2861 7862 7891 2861 Size: 5001 29 5750 Fragments arranged by size: 5750 5001 29 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8017 9787 809 Size: 507 6701 1770 1802 Fragments arranged by size: 6701 1802 1770 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2276 2897 3210 Size: 54 1425 50 275 18 446 621 313 Cuts at: 3210 3594 6060 7261 7435 8227 9116 10745 8 Size: 384 2466 1201 174 792 889 1629 43 Fragments arranged by size: 2466 1629 1425 1201 889 792 621 446 384 313 275 174 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8227 1537 Size: 275 6415 4090 Fragments arranged by size: 6415 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2718 3867 4313 4401 5833 6564 6582 6858 Size: 1365 1149 446 88 1432 731 18 276 Cuts at: 6858 6891 8010 8026 8077 8710 1353 Size: 33 1119 16 51 633 3423 Fragments arranged by size: 3423 1432 1365 1149 1119 731 633 446 276 88 51 33 18 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2054 3257 9367 9741 9887 387 Size: 1064 138 465 1203 6110 374 146 1280 Fragments arranged by size: 6110 1280 1203 1064 465 374 146 138 EcoICRI GAG'CTC Cuts at: 2819 2819 Size: 10780 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2906 3693 3855 3921 4171 5112 5537 Size: 393 1274 787 162 66 250 941 425 Cuts at: 5537 5670 6743 7025 7233 7422 7476 8843 9440 Size: 133 1073 282 208 189 54 1367 597 Cuts at: 9440 10227 10495 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1367 1274 1073 941 787 787 597 425 393 282 268 250 208 189 162 133 66 54 EcoNI CCTnn'n_nnAGG Cuts at: 5844 5844 Size: 10780 EcoO109I rG'GnC_Cy Cuts at: 2956 3114 3721 6580 7770 2956 Size: 158 607 2859 1190 5966 Fragments arranged by size: 5966 2859 1190 607 158 EcoRI G'AATT_C Cuts at: 4461 4979 5240 8089 8467 4461 Size: 518 261 2849 378 6774 Fragments arranged by size: 6774 2849 518 378 261 EcoRV GAT'ATC Cuts at: 5986 5986 Size: 10780 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3421 4556 6536 3421 Size: 1135 1980 7665 Fragments arranged by size: 7665 1980 1135 FspI TGC'GCA Cuts at: 9286 9286 Size: 10780 HaeII r_GCGC'y Cuts at: 1678 3531 7980 9927 1678 Size: 1853 4449 1947 2531 Fragments arranged by size: 4449 2531 1947 1853 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3653 3685 3811 3843 4868 Size: 32 135 32 2081 32 126 32 1025 Cuts at: 4868 4880 4900 4912 5330 5362 5645 5677 5970 Size: 12 20 12 418 32 283 32 293 Cuts at: 5970 6002 7040 7072 7541 7573 9421 9453 9495 Size: 32 1038 32 469 32 1848 32 42 Cuts at: 9495 9527 1373 Size: 32 2626 Fragments arranged by size: 2626 2081 1848 1038 1025 469 418 293 283 135 126 42 32 32 32 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 3843 8124 8370 669 Size: 988 281 1905 4281 246 3079 Fragments arranged by size: 4281 3079 1905 988 281 246 HindIII A'AGCT_T Cuts at: 748 3478 748 Size: 2730 8050 HpaI GTT'AAC Cuts at: 3843 8370 3843 Size: 4527 6253 KpnI G_GTAC'C Cuts at: 8105 8105 Size: 10780 MfeI C'AATT_G Cuts at: 2562 2569 3584 8379 2562 Size: 7 1015 4795 4963 Fragments arranged by size: 4963 4795 1015 7 MluI A'CGCG_T Cuts at: 10656 10656 Size: 10780 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5802 9894 10078 1305 Size: 4497 4092 184 2007 Fragments arranged by size: 4497 4092 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2278 2899 3212 3596 6062 Size: 54 1425 343 446 621 313 384 2466 Cuts at: 6062 7263 7437 10 Size: 1201 174 3353 Fragments arranged by size: 3353 2466 1425 1201 621 446 384 343 313 174 54 MslI CAynn'nnrTG Cuts at: 518 1166 2073 2328 6014 6174 7760 8738 9097 Size: 648 907 255 3686 160 1586 978 359 Cuts at: 9097 9256 518 Size: 159 2042 Fragments arranged by size: 3686 2042 1586 978 907 648 359 255 160 159 MspA1I CmG'CkG Cuts at: 1606 4038 5004 5633 7414 8196 8873 10021 10266 Size: 2432 966 629 1781 782 677 1148 245 Cuts at: 10266 1606 Size: 2120 Fragments arranged by size: 2432 2120 1781 1148 966 782 677 629 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3973 513 Size: 552 939 1969 7320 Fragments arranged by size: 7320 1969 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10780 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10780 NotI GC'GGCC_GC Cuts at: 8227 8227 Size: 10780 NspI r_CATG'y Cuts at: 1132 1229 3035 4679 8138 9683 1132 Size: 97 1806 1644 3459 1545 2229 Fragments arranged by size: 3459 2229 1806 1644 1545 97 PasI CC'CwG_GG Cuts at: 1348 7352 7777 1348 Size: 6004 425 4351 Fragments arranged by size: 6004 4351 425 PciI A'CATG_T Cuts at: 9679 9679 Size: 10780 PflMI CCAn_nnn'nTGG Cuts at: 1108 7391 1108 Size: 6283 4497 PfoI T'CCnGG_A Cuts at: 3832 7492 3832 Size: 3660 7120 PmeI GTTT'AAAC Cuts at: 8084 8084 Size: 10780 PmlI CAC'GTG Cuts at: 2071 2351 6385 2071 Size: 280 4034 6466 Fragments arranged by size: 6466 4034 280 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7622 7654 8849 8881 10362 10394 7622 Size: 32 1195 32 1481 32 8008 Fragments arranged by size: 8008 1481 1195 32 32 32 PpuMI rG'GwC_Cy Cuts at: 6580 7770 6580 Size: 1190 9590 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10780 PsiI TTA'TAA Cuts at: 8350 8350 Size: 10780 PspOMI G'GGCC_C Cuts at: 2955 2955 Size: 10780 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4416 4448 6786 6818 4416 Size: 32 2338 32 8378 Fragments arranged by size: 8378 2338 32 32 PstI C_TGCA'G Cuts at: 830 2207 5197 5404 6263 6925 7039 8132 830 Size: 1377 2990 207 859 662 114 1093 3478 Fragments arranged by size: 3478 2990 1377 1093 859 662 207 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10780 PvuII CAG'CTG Cuts at: 4038 5633 7414 4038 Size: 1595 1781 7404 Fragments arranged by size: 7404 1781 1595 SacI G_AGCT'C Cuts at: 2821 2821 Size: 10780 SalI G'TCGA_C Cuts at: 1936 8122 1936 Size: 6186 4594 SapI GCTCTTCn'nnn_ Cuts at: 4401 8077 4401 Size: 3676 7104 SbfI CC_TGCA'GG Cuts at: 8132 8132 Size: 10780 ScaI AGT'ACT Cuts at: 4049 9028 10740 4049 Size: 4979 1712 4089 Fragments arranged by size: 4979 4089 1712 SexAI A'CCwGG_T Cuts at: 6022 7766 6022 Size: 1744 9036 SfcI C'TryA_G Cuts at: 826 1045 2203 4422 4466 4857 5193 5400 6259 Size: 219 1158 2219 44 391 336 207 859 Cuts at: 6259 6921 6971 7035 8128 9263 9944 10135 826 Size: 662 50 64 1093 1135 681 191 1471 Fragments arranged by size: 2219 1471 1158 1135 1093 859 681 662 391 336 219 207 191 64 50 44 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10780 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10780 SmaI CCC'GGG Cuts at: 1872 8107 1872 Size: 6235 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2387 4378 5537 5903 6224 6557 Size: 197 926 444 1991 1159 366 321 333 Cuts at: 6557 8884 9785 10047 820 Size: 2327 901 262 1553 Fragments arranged by size: 2327 1991 1553 1159 926 901 444 366 333 321 262 197 SnaBI TAC'GTA Cuts at: 493 5961 8571 493 Size: 5468 2610 2702 Fragments arranged by size: 5468 2702 2610 SpeI A'CTAG_T Cuts at: 152 152 Size: 10780 SphI G_CATG'C Cuts at: 1132 8138 1132 Size: 7006 3774 SspI AAT'ATT Cuts at: 5 52 4668 8704 5 Size: 47 4616 4036 2081 Fragments arranged by size: 4616 4036 2081 47 StuI AGG'CCT Cuts at: 1517 3539 1517 Size: 2022 8758 StyI C'CwwG_G Cuts at: 513 1065 2004 3125 3643 3973 5055 5373 6057 Size: 552 939 1121 518 330 1082 318 684 Cuts at: 6057 7264 7818 8219 513 Size: 1207 554 401 3074 Fragments arranged by size: 3074 1207 1121 1082 939 684 554 552 518 401 330 318 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 4411 6005 7825 7850 8807 8824 Size: 1255 558 2273 1594 1820 25 957 17 Cuts at: 8824 8977 9162 9607 325 Size: 153 185 445 1498 Fragments arranged by size: 2273 1820 1594 1498 1255 957 558 445 185 153 25 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4047 5006 6036 Size: 274 80 33 51 1443 2070 959 1030 Cuts at: 6036 9026 10738 96 Size: 2990 1712 138 Fragments arranged by size: 2990 2070 1712 1443 1030 959 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6940 6972 7210 7242 10740 10772 6940 Size: 32 238 32 3498 32 6948 Fragments arranged by size: 6948 3498 238 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 7768 1316 Size: 6452 4328 XbaI T'CTAG_A Cuts at: 3781 5789 7687 8116 3781 Size: 2008 1898 429 6445 Fragments arranged by size: 6445 2008 1898 429 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6776 6776 Size: 10780 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10780 XmaI C'CCGG_G Cuts at: 1870 8105 1870 Size: 6235 4545 XmnI GAAnn'nnTTC Cuts at: 4242 5125 6345 6999 7080 7683 8909 4242 Size: 883 1220 654 81 603 1226 6113 Fragments arranged by size: 6113 1226 1220 883 654 603 81 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8588 276 Size: 53 83 186 936 7054 2468 Fragments arranged by size: 7054 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI RsrII SacII SanDI SfiI SfoI SrfI SwaI