################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AlwNI AocI Aor51HI AscI Asp700I Asp718I AvrII BamHI BanI BbeI BbrPI BcgI BciVI BfrI BlnI BsaAI BsaHI BseAI BseRI BsiWI BsmI BspDI BspEI BspHI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI Eco47III Eco81I EcoNI EcoO65I EcoT22I FauI FseI HaeII HgaI Hin1I HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 347 GTCGAC GT^[AC][GT]AC AflIII 3444 ACATGT A^C[GA][CT]GT ApaI 1482 GGGCCC GGGCC^C AseI 1987 ATTAAT AT^TAAT AsnI 1987 ATTAAT AT^TAAT AspI 2529 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 929 CTCGAG C^[CT]CG[GA]G AviII 2198 TGCGCA TGC^GCA BalI 1797 TGGCCA TGG^CCA BclI 1040 TGATCA T^GATCA BglI 1103 GCCATTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2845 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2845 GCTCAGC GC^T[ACGT]AGC BsaBI 1681 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 843 TCCGGT [AT]^CCGG[AT] BsiEI 40 CGGCCG CG[GA][CT]^CG BsmBI 2534 CGTCTC CGTCTC(1/5) BsoBI 929 CTCGAG C^[CT]CG[GA]G BspLU11I 3444 ACATGT A^CATGT BspMI 3159 ACCTGC ACCTGC(4/8) BsrDI 2853 CATTGC GCAATG(2/0) BstAPI 2567 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 2845 GCTCAGC GC^T[ACGT]AGC DrdI 2526 GACGACCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 40 CGGCCG C^GGCCG EclXI 40 CGGCCG C^GGCCG Eco52I 40 CGGCCG C^GGCCG FbaI 1040 TGATCA T^GATCA FspI 2198 TGCGCA TGC^GCA MamI 1681 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1797 TGGCCA TGG^CCA MscI 1797 TGGCCA TGG^CCA MslI 2452 CATTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PaeR7I 929 CTCGAG C^TCGAG PflFI 2529 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 434 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 2656 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1987 ATTAAT AT^TAAT SalI 347 GTCGAC G^TCGAC SapI 577 GAAGAGC GCTCTTC(1/4) StuI 2254 AGGCCT AGG^CCT Tth111I 2529 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 936 TCTAGA T^CTAGA XhoI 929 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AhdI 241 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 450 GACTGCTGGTC AlwI 1843 GATCC GGATC(4/5) 3233 GGATC AspEI 241 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 450 GACTGCTGGTC BglII 1544 AGATCT A^GATCT 2490 AGATCT BsaI 1356 GAGACC GGTCTC(1/5) 3156 GAGACC BsgI 659 CTGCAC GTGCAG(16/14) 2639 CTGCAC Bsp1407I 521 TGTACA T^GTACA 547 TGTACA BsrBI 784 GAGCGG CCGCTC(-3/-3) 2521 GAGCGG BsrFI 2090 GCCGGC [GA]^CCGG[CT] 3362 GCCGGC BsrGI 521 TGTACA T^GTACA 547 TGTACA BssSI 2560 CACGAG CACGAG(-5/-1) 3228 CTCGTG Cfr10I 2090 GCCGGC [GA]^CCGG[CT] 3362 GCCGGC DraII 434 AGGTCCC [GA]G^G[ACGT]CC[CT] 1482 GGGCCCT DraIII 956 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 2568 CACTGTGTG DsaI 704 CCATGG C^C[GA][CT]GG 1523 CCATGG EaeI 40 CGGCCG [CT]^GGCC[GA] 1797 TGGCCA Eam1105I 241 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 450 GACTGCTGGTC EarI 577 GAAGAG CTCTTC(1/4) 2154 GAAGAG EcoO109I 434 AGGTCCC [GA]G^G[ACGT]CC[CT] 1482 GGGCCCT HincII 347 GTCGAC GT[CT]^[GA]AC 2663 GTCAAC HindII 347 GTCGAC GT[CT]^[GA]AC 2663 GTCAAC Ksp632I 577 GAAGAG CTCTTC(1/4) 2154 GAAGAG MseI 1900 TTAA T^TAA 1988 TTAA MspA1I 682 CAGCTG C[AC]G^C[GT]G 1826 CAGCTG NaeI 2090 GCCGGC GCC^GGC 3362 GCCGGC NcoI 704 CCATGG C^CATGG 1523 CCATGG NgoMIV 2090 GCCGGC G^CCGGC 3362 GCCGGC NspI 3432 ACATGC [GA]CATG^[CT] 3444 ACATGT PflMI 2947 CCATATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3343 CCAACCAGTGG PstI 793 CTGCAG CTGCA^G 816 CTGCAG PvuII 682 CAGCTG CAG^CTG 1826 CAGCTG SspBI 521 TGTACA T^GTACA 547 TGTACA SspI 1459 AATATT AAT^ATT 3356 AATATT Tru9I 1900 TTAA T^TAA 1988 TTAA Van91I 2947 CCATATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3343 CCAACCAGTGG XcmI 461 CCAAGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2540 CCAAAAGGCTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AcsI AfaI AluI Alw44I ApaLI ApoI AspHI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRI EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII