(Circular) (Six-base) MAPSORT of: pFN21AE3381.seq Check: 5733 from: 1 to: 8158 pFN21AE3381 8158 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5968 278 Size: 53 83 186 936 4432 2468 Fragments arranged by size: 4432 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2353 5501 1937 Size: 416 3148 4594 Fragments arranged by size: 4594 3148 416 Acc65I G'GTAC_C Cuts at: 5479 5479 Size: 8158 AclI AA'CG_TT Cuts at: 1563 6285 6658 1563 Size: 4722 373 3063 Fragments arranged by size: 4722 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3237 3528 3801 4075 4178 5105 6221 7605 Size: 1605 291 273 274 103 927 1116 1384 Cuts at: 7605 7873 1632 Size: 268 1917 Fragments arranged by size: 1917 1605 1384 1116 927 291 274 273 268 103 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7961 AflIII A'CryG_T Cuts at: 1203 5449 7057 8034 1203 Size: 4246 1608 977 1327 Fragments arranged by size: 4246 1608 1327 977 AgeI A'CCGG_T Cuts at: 6989 6989 Size: 8158 AhdI GACnn_n'nnGTC Cuts at: 2251 2460 6887 2251 Size: 209 4427 3522 Fragments arranged by size: 4427 3522 209 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8158 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8158 AlwNI CAG_nnn'CTG Cuts at: 5901 7473 5901 Size: 1572 6586 ApaI G_GGCC'C Cuts at: 3491 3491 Size: 8158 ApaLI G'TGCA_C Cuts at: 2911 3698 4570 6215 7371 2911 Size: 787 872 1645 1156 3698 Fragments arranged by size: 3698 1645 1156 872 787 ApoI r'AATT_y Cuts at: 1493 2150 2343 2956 2984 4627 4765 5283 5467 Size: 657 193 613 28 1643 138 518 184 Cuts at: 5467 5693 5845 7954 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1643 657 613 518 226 193 184 152 138 28 AseI AT'TA_AT Cuts at: 160 1032 3993 6712 160 Size: 872 2961 2719 1606 Fragments arranged by size: 2961 2719 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8158 AvaI C'yCGr_G Cuts at: 1870 1943 2934 5483 1870 Size: 73 991 2549 4545 Fragments arranged by size: 4545 2549 991 73 BamHI G'GATC_C Cuts at: 5488 5855 5488 Size: 367 7791 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5479 6934 618 Size: 325 217 627 3692 1455 1842 Fragments arranged by size: 3692 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2941 3156 3305 3426 3491 5477 1391 Size: 1550 215 149 121 65 1986 4072 Fragments arranged by size: 4072 1986 1550 215 149 121 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2432 2508 2874 3225 5220 928 Size: 971 533 76 366 351 1995 3866 Fragments arranged by size: 3866 1995 971 533 366 351 76 BbvCI CC'TCA_GC Cuts at: 2697 5205 5290 2697 Size: 2508 85 5565 Fragments arranged by size: 5565 2508 85 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6349 6383 1903 Size: 34 4412 34 3678 Fragments arranged by size: 4412 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6050 7260 6050 Size: 1210 6948 BclI T'GATC_A Cuts at: 1548 3045 1548 Size: 1497 6661 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3114 6769 136 Size: 107 122 71 631 605 1442 3655 1525 Fragments arranged by size: 3655 1525 1442 631 605 122 107 71 BglII A'GATC_T Cuts at: 3549 4495 8153 3549 Size: 946 3658 3554 Fragments arranged by size: 3658 3554 946 BlpI GC'TnA_GC Cuts at: 4851 5575 4851 Size: 724 7434 Bme1580I G_kGCm'C Cuts at: 1790 2878 2900 2915 3491 3702 4491 4574 6219 Size: 1088 22 15 576 211 789 83 1645 Cuts at: 6219 7375 1790 Size: 1156 2573 Fragments arranged by size: 2573 1645 1156 1088 789 576 211 83 22 15 BmgBI CAC'GTC Cuts at: 1318 4486 4539 1318 Size: 3168 53 4937 Fragments arranged by size: 4937 3168 53 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2302 4017 5889 Size: 416 53 83 399 24 885 1715 1872 Cuts at: 5889 6847 442 Size: 958 1753 Fragments arranged by size: 1872 1753 1715 958 885 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8158 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5290 5290 Size: 8158 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2295 2333 3435 3955 4932 6818 1239 Size: 1056 38 1102 520 977 1886 2579 Fragments arranged by size: 2579 1886 1102 1056 977 520 38 Bpu10I CC'TnA_GC Cuts at: 2436 2697 4245 5205 5290 2436 Size: 261 1548 960 85 5304 Fragments arranged by size: 5304 1548 960 261 85 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2599 3066 3522 4308 6283 7148 7446 2599 Size: 467 456 786 1975 865 298 3311 Fragments arranged by size: 3311 1975 865 786 467 456 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3355 5155 6821 882 Size: 617 1856 1800 1666 2219 Fragments arranged by size: 2219 1856 1800 1666 617 BsaAI yAC'GTr Cuts at: 493 1204 5949 493 Size: 711 4745 2702 Fragments arranged by size: 4745 2702 711 BsaBI GATnn'nnATC Cuts at: 3690 8152 3690 Size: 4462 3696 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5965 6347 6982 275 Size: 53 83 186 936 4432 382 635 1451 Fragments arranged by size: 4432 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2848 6591 6989 7263 7410 7821 2848 Size: 3743 398 274 147 411 3185 Fragments arranged by size: 3743 3185 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2093 2123 3053 3083 4525 Size: 30 165 30 690 30 930 30 1442 Cuts at: 4525 4555 1178 Size: 30 4781 Fragments arranged by size: 4781 1442 930 690 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 8158 BseYI C'CCAG_C Cuts at: 3829 7361 7857 3829 Size: 3532 496 4130 Fragments arranged by size: 4130 3532 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2649 4629 1163 Size: 573 913 1980 4692 Fragments arranged by size: 4692 1980 913 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2048 5608 6369 7397 1540 Size: 275 186 47 3560 761 1028 2301 Fragments arranged by size: 3560 2301 1028 761 275 186 47 BsiHKAI G_wGCw'C Cuts at: 2915 2941 3156 3305 3702 4574 6219 6304 7375 Size: 26 215 149 397 872 1645 85 1071 Cuts at: 7375 2915 Size: 3698 Fragments arranged by size: 3698 1645 1071 872 397 215 149 85 26 BsmI GAATG_Cn' Cuts at: 5669 5762 5669 Size: 93 8065 BsmBI CGTCTCn'nnnn_ Cuts at: 4545 4545 Size: 8158 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2878 2900 2915 2941 3156 3305 3426 Size: 399 1088 22 15 26 215 149 121 Cuts at: 3426 3491 3702 4491 4574 5477 6219 6304 7375 Size: 65 211 789 83 903 742 85 1071 Cuts at: 7375 1391 Size: 2174 Fragments arranged by size: 2174 1088 1071 903 789 742 399 215 211 149 121 85 83 65 26 22 15 BspEI T'CCGG_A Cuts at: 7821 7821 Size: 8158 BspHI T'CATG_A Cuts at: 6047 7777 6047 Size: 1730 6428 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5173 5513 844 Size: 4329 340 3489 Fragments arranged by size: 4329 3489 340 BsrBI CCG'CTC Cuts at: 1394 1823 2791 4528 6045 1394 Size: 429 968 1737 1517 3507 Fragments arranged by size: 3507 1737 1517 968 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4857 6653 6827 65 Size: 1183 3609 1796 174 1396 Fragments arranged by size: 3609 1796 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4095 5367 6802 6989 1700 Size: 110 2285 1272 1435 187 2869 Fragments arranged by size: 2869 2285 1435 1272 187 110 BsrGI T'GTAC_A Cuts at: 96 2526 2552 96 Size: 2430 26 5702 Fragments arranged by size: 5702 2430 26 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8158 BssSI C'ACGA_G Cuts at: 4565 5233 6218 7230 4565 Size: 668 985 1012 5493 Fragments arranged by size: 5493 1012 985 668 BstAPI GCAn_nnn'nTGC Cuts at: 794 4578 794 Size: 3784 4374 BstBI TT'CG_AA Cuts at: 6962 6962 Size: 8158 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8158 BstYI r'GATC_y Cuts at: 1972 3549 4495 5238 5488 5855 6240 6257 7709 Size: 1577 946 743 250 367 385 17 1452 Cuts at: 7709 7795 7807 8153 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1577 1452 946 743 385 367 346 250 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2709 3528 513 Size: 552 1644 819 5143 Fragments arranged by size: 5143 1644 819 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7419 Fragments arranged by size: 7419 683 56 BtsI GCAGTG_nn' Cuts at: 2830 3030 3103 5674 6468 6488 2830 Size: 200 73 2571 794 20 4500 Fragments arranged by size: 4500 2571 794 200 73 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8158 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2134 2169 2716 2749 2751 2784 529 Size: 35 1570 35 547 33 2 33 5903 Fragments arranged by size: 5903 1570 547 35 35 33 33 2 DraI TTT'AAA Cuts at: 5462 5462 Size: 8158 DraIII CAC_nnn'GTG Cuts at: 2966 4578 2966 Size: 1612 6546 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4537 7165 809 Size: 507 3221 2628 1802 Fragments arranged by size: 3221 2628 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2045 3802 5605 Size: 54 1425 50 275 18 215 1757 1803 Cuts at: 5605 6494 8123 8 Size: 889 1629 43 Fragments arranged by size: 1803 1757 1629 1425 889 275 215 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2045 5605 1537 Size: 275 233 3560 4090 Fragments arranged by size: 4090 3560 275 233 EarI CTCTTCn'nnn_ Cuts at: 1353 2577 4154 6088 1353 Size: 1224 1577 1934 3423 Fragments arranged by size: 3423 1934 1577 1224 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6745 7119 7265 387 Size: 1064 138 5156 374 146 1280 Fragments arranged by size: 5156 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2939 3154 3303 2939 Size: 215 149 7794 Fragments arranged by size: 7794 215 149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2295 2333 3237 3435 3528 3801 3955 Size: 393 663 38 904 198 93 273 154 Cuts at: 3955 4075 4178 4932 5105 6221 6818 7605 7873 Size: 120 103 754 173 1116 597 787 268 Cuts at: 7873 1239 Size: 1524 Fragments arranged by size: 1524 1116 904 787 754 663 597 393 273 268 198 173 154 120 103 93 38 EcoO109I rG'GnC_Cy Cuts at: 2440 3488 2440 Size: 1048 7110 EcoRI G'AATT_C Cuts at: 2956 2984 5283 5467 5845 2956 Size: 28 2299 184 378 5269 Fragments arranged by size: 5269 2299 378 184 28 EcoRV GAT'ATC Cuts at: 3523 4372 5380 3523 Size: 849 1008 6301 Fragments arranged by size: 6301 1008 849 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2422 3417 2422 Size: 995 7163 FspI TGC'GCA Cuts at: 4205 6664 4205 Size: 2459 5699 FspAI rTGC'GCAy Cuts at: 4205 4205 Size: 8158 HaeII r_GCGC'y Cuts at: 1678 7305 1678 Size: 5627 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 2237 2269 2419 Size: 32 135 32 521 32 112 32 150 Cuts at: 2419 2446 2451 2478 2597 2629 3287 3319 4356 Size: 27 5 27 119 32 658 32 1037 Cuts at: 4356 4388 4525 4557 5210 5242 6799 6831 6873 Size: 32 137 32 653 32 1557 32 42 Cuts at: 6873 6905 1373 Size: 32 2626 Fragments arranged by size: 2626 1557 1037 658 653 521 150 137 135 119 112 42 32 32 32 32 32 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 2354 4670 5502 5748 669 Size: 988 281 416 2316 832 246 3079 Fragments arranged by size: 3079 2316 988 832 416 281 246 HindIII A'AGCT_T Cuts at: 748 2137 3394 3540 3783 4074 748 Size: 1389 1257 146 243 291 4832 Fragments arranged by size: 4832 1389 1257 291 243 146 HpaI GTT'AAC Cuts at: 5748 5748 Size: 8158 KpnI G_GTAC'C Cuts at: 5483 5483 Size: 8158 MfeI C'AATT_G Cuts at: 5757 5757 Size: 8158 MluI A'CGCG_T Cuts at: 8034 8034 Size: 8158 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4197 5372 7272 7456 1305 Size: 2892 1175 1900 184 2007 Fragments arranged by size: 2892 2007 1900 1175 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3804 10 Size: 54 1425 343 1972 4364 Fragments arranged by size: 4364 1972 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4461 6116 6475 6634 518 Size: 648 3295 1655 359 159 2042 Fragments arranged by size: 3295 2042 1655 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2689 3833 5574 6251 7399 7644 1606 Size: 1083 1144 1741 677 1148 245 2120 Fragments arranged by size: 2120 1741 1148 1144 1083 677 245 NaeI GCC'GGC Cuts at: 4097 5369 4097 Size: 1272 6886 NcoI C'CATG_G Cuts at: 513 1065 2709 3528 513 Size: 552 1644 819 5143 Fragments arranged by size: 5143 1644 819 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8158 NgoMIV G'CCGG_C Cuts at: 4095 5367 4095 Size: 1272 6886 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8158 NotI GC'GGCC_GC Cuts at: 5605 5605 Size: 8158 NspI r_CATG'y Cuts at: 1132 1229 5441 5453 5516 7061 1132 Size: 97 4212 12 63 1545 2229 Fragments arranged by size: 4212 2229 1545 97 63 12 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8158 PciI A'CATG_T Cuts at: 5449 7057 5449 Size: 1608 6550 PflMI CCAn_nnn'nTGG Cuts at: 1108 4958 5354 1108 Size: 3850 396 3912 Fragments arranged by size: 3912 3850 396 PfoI T'CCnGG_A Cuts at: 2220 4378 2220 Size: 2158 6000 PmeI GTTT'AAAC Cuts at: 5462 5462 Size: 8158 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6227 6259 7740 7772 6227 Size: 32 1481 32 6613 Fragments arranged by size: 6613 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2440 2440 Size: 8158 PshAI GACnn'nnGTC Cuts at: 1613 4665 1613 Size: 3052 5106 PsiI TTA'TAA Cuts at: 5408 5728 5408 Size: 320 7838 PspOMI G'GGCC_C Cuts at: 3487 3487 Size: 8158 PspXI vC'TCGA_Gb Cuts at: 2934 2934 Size: 8158 PstI C_TGCA'G Cuts at: 830 2802 2825 5510 830 Size: 1972 23 2685 3478 Fragments arranged by size: 3478 2685 1972 23 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8158 PvuII CAG'CTG Cuts at: 2689 3833 2689 Size: 1144 7014 SacI G_AGCT'C Cuts at: 2941 3156 3305 2941 Size: 215 149 7794 Fragments arranged by size: 7794 215 149 SalI G'TCGA_C Cuts at: 1936 2352 5500 1936 Size: 416 3148 4594 Fragments arranged by size: 4594 3148 416 SapI GCTCTTCn'nnn_ Cuts at: 2577 2577 Size: 8158 SbfI CC_TGCA'GG Cuts at: 5510 5510 Size: 8158 ScaI AGT'ACT Cuts at: 6406 8118 6406 Size: 1712 6446 SfcI C'TryA_G Cuts at: 826 1045 2798 2821 3022 5506 6641 7322 7513 Size: 219 1753 23 201 2484 1135 681 191 Cuts at: 7513 826 Size: 1471 Fragments arranged by size: 2484 1753 1471 1135 681 219 201 191 23 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8158 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8158 SmaI CCC'GGG Cuts at: 1872 5485 1872 Size: 3613 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2614 2934 3081 3537 4323 6262 Size: 197 926 671 320 147 456 786 1939 Cuts at: 6262 7163 7425 820 Size: 901 262 1553 Fragments arranged by size: 1939 1553 926 901 786 671 456 320 262 197 147 SnaBI TAC'GTA Cuts at: 493 5949 493 Size: 5456 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8158 SphI G_CATG'C Cuts at: 1132 5516 1132 Size: 4384 3774 SspI AAT'ATT Cuts at: 5 52 3466 5363 6082 5 Size: 47 3414 1897 719 2081 Fragments arranged by size: 3414 2081 1897 719 47 StuI AGG'CCT Cuts at: 1517 4261 1517 Size: 2744 5414 StyI C'CwwG_G Cuts at: 513 1065 2709 3528 3639 5597 513 Size: 552 1644 819 111 1958 3074 Fragments arranged by size: 3074 1958 1644 819 552 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4374 5061 6185 6202 6355 6540 6985 Size: 1255 2794 687 1124 17 153 185 445 Cuts at: 6985 325 Size: 1498 Fragments arranged by size: 2794 1498 1255 1124 687 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2526 2552 2814 Size: 274 80 33 51 1443 549 26 262 Cuts at: 2814 6404 8116 96 Size: 3590 1712 138 Fragments arranged by size: 3590 1712 1443 549 274 262 138 80 51 33 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3053 3085 8118 8150 3053 Size: 32 5033 32 3061 Fragments arranged by size: 5033 3061 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4537 1316 Size: 3221 4937 XbaI T'CTAG_A Cuts at: 2941 5494 2941 Size: 2553 5605 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2473 4552 2473 Size: 2079 6079 XhoI C'TCGA_G Cuts at: 1943 2934 1943 Size: 991 7167 XmaI C'CCGG_G Cuts at: 1870 5483 1870 Size: 3613 4545 XmnI GAAnn'nnTTC Cuts at: 6287 6287 Size: 8158 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5966 276 Size: 53 83 186 936 4432 2468 Fragments arranged by size: 4432 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoNI FseI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI