(Linear) (Six-base) MAPSORT of: KIEE3381.seq Check: 1229 from: 1 to: 3453 KIEE3381.orf With 182 enzymes: SgfI * July 3, 2012 17:48 .. AccI GT'mk_AC Cuts at: 0 348 3453 Size: 348 3105 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1232 1523 1796 2070 2173 3100 3453 Size: 1232 291 273 274 103 927 353 Fragments arranged by size: 1232 927 353 291 274 273 103 AflIII A'CryG_T Cuts at: 0 3444 3453 Size: 3444 9 AhdI GACnn_n'nnGTC Cuts at: 0 246 455 3453 Size: 246 209 2998 Fragments arranged by size: 2998 246 209 ApaI G_GGCC'C Cuts at: 0 1486 3453 Size: 1486 1967 ApaLI G'TGCA_C Cuts at: 0 906 1693 2565 3453 Size: 906 787 872 888 Fragments arranged by size: 906 888 872 787 ApoI r'AATT_y Cuts at: 0 145 338 951 979 2622 2760 3278 3453 Size: 145 193 613 28 1643 138 518 175 Fragments arranged by size: 1643 613 518 193 175 145 138 28 AseI AT'TA_AT Cuts at: 0 1988 3453 Size: 1988 1465 AvaI C'yCGr_G Cuts at: 0 929 3453 Size: 929 2524 BanII G_rGCy'C Cuts at: 0 936 1151 1300 1421 1486 3453 Size: 936 215 149 121 65 1967 Fragments arranged by size: 1967 936 215 149 121 65 BbsI GAAGACnn'nnnn_ Cuts at: 0 427 503 869 1220 3215 3453 Size: 427 76 366 351 1995 238 Fragments arranged by size: 1995 427 366 351 238 76 BbvCI CC'TCA_GC Cuts at: 0 692 3200 3285 3453 Size: 692 2508 85 168 Fragments arranged by size: 2508 692 168 85 BclI T'GATC_A Cuts at: 0 1040 3453 Size: 1040 2413 BglI GCCn_nnn'nGGC Cuts at: 0 1109 3453 Size: 1109 2344 BglII A'GATC_T Cuts at: 0 1544 2490 3453 Size: 1544 946 963 Fragments arranged by size: 1544 963 946 BlpI GC'TnA_GC Cuts at: 0 2846 3453 Size: 2846 607 Bme1580I G_kGCm'C Cuts at: 0 873 895 910 1486 1697 2486 2569 3453 Size: 873 22 15 576 211 789 83 884 Fragments arranged by size: 884 873 789 576 211 83 22 15 BmgBI CAC'GTC Cuts at: 0 2481 2534 3453 Size: 2481 53 919 Fragments arranged by size: 2481 919 53 BmrI ACTGGGnnnn_n' Cuts at: 0 297 2012 3453 Size: 297 1715 1441 Fragments arranged by size: 1715 1441 297 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3285 3453 Size: 3285 168 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 290 328 1430 1950 2927 3453 Size: 290 38 1102 520 977 526 Fragments arranged by size: 1102 977 526 520 290 38 Bpu10I CC'TnA_GC Cuts at: 0 431 692 2240 3200 3285 3453 Size: 431 261 1548 960 85 168 Fragments arranged by size: 1548 960 431 261 168 85 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 594 1061 1517 2303 3453 Size: 594 467 456 786 1150 Fragments arranged by size: 1150 786 594 467 456 BsaI GGTCTCn'nnnn_ Cuts at: 0 1350 3150 3453 Size: 1350 1800 303 Fragments arranged by size: 1800 1350 303 BsaBI GATnn'nnATC Cuts at: 0 1685 3453 Size: 1685 1768 BsaWI w'CCGG_w Cuts at: 0 843 3453 Size: 843 2610 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 88 118 1048 1078 2520 2550 3453 Size: 88 30 930 30 1442 30 903 Fragments arranged by size: 1442 930 903 88 30 30 30 BseYI C'CCAG_C Cuts at: 0 1824 3453 Size: 1824 1629 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 644 2624 3453 Size: 644 1980 829 Fragments arranged by size: 1980 829 644 BsiEI CG_ry'CG Cuts at: 0 43 3453 Size: 43 3410 BsiHKAI G_wGCw'C Cuts at: 0 910 936 1151 1300 1697 2569 3453 Size: 910 26 215 149 397 872 884 Fragments arranged by size: 910 884 872 397 215 149 26 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2540 3453 Size: 2540 913 Bsp1286I G_dGCh'C Cuts at: 0 873 895 910 936 1151 1300 1421 1486 Size: 873 22 15 26 215 149 121 65 Cuts at: 1486 1697 2486 2569 3453 Size: 211 789 83 884 Fragments arranged by size: 884 873 789 215 211 149 121 83 65 26 22 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3168 3453 Size: 3168 285 BsrBI CCG'CTC Cuts at: 0 786 2523 3453 Size: 786 1737 930 Fragments arranged by size: 1737 930 786 BsrDI GCAATG_nn' Cuts at: 0 2852 3453 Size: 2852 601 BsrFI r'CCGG_y Cuts at: 0 2090 3362 3453 Size: 2090 1272 91 Fragments arranged by size: 2090 1272 91 BsrGI T'GTAC_A Cuts at: 0 521 547 3453 Size: 521 26 2906 Fragments arranged by size: 2906 521 26 BssSI C'ACGA_G Cuts at: 0 2560 3228 3453 Size: 2560 668 225 Fragments arranged by size: 2560 668 225 BstAPI GCAn_nnn'nTGC Cuts at: 0 2573 3453 Size: 2573 880 BstYI r'GATC_y Cuts at: 0 1544 2490 3233 3453 Size: 1544 946 743 220 Fragments arranged by size: 1544 946 743 220 BtgI C'CryG_G Cuts at: 0 704 1523 3453 Size: 704 819 1930 Fragments arranged by size: 1930 819 704 BtsI GCAGTG_nn' Cuts at: 0 825 1025 1098 3453 Size: 825 200 73 2355 Fragments arranged by size: 2355 825 200 73 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 129 164 711 744 746 779 3453 Size: 129 35 547 33 2 33 2674 Fragments arranged by size: 2674 547 129 35 33 33 2 DraIII CAC_nnn'GTG Cuts at: 0 961 2573 3453 Size: 961 1612 880 Fragments arranged by size: 1612 961 880 DrdI GACnn_nn'nnGTC Cuts at: 0 2532 3453 Size: 2532 921 EaeI y'GGCC_r Cuts at: 0 40 1797 3453 Size: 40 1757 1656 Fragments arranged by size: 1757 1656 40 EagI C'GGCC_G Cuts at: 0 40 3453 Size: 40 3413 EarI CTCTTCn'nnn_ Cuts at: 0 572 2149 3453 Size: 572 1577 1304 Fragments arranged by size: 1577 1304 572 EcoICRI GAG'CTC Cuts at: 0 934 1149 1298 3453 Size: 934 215 149 2155 Fragments arranged by size: 2155 934 215 149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 290 328 1232 1430 1523 1796 1950 2070 Size: 290 38 904 198 93 273 154 120 Cuts at: 2070 2173 2927 3100 3453 Size: 103 754 173 353 Fragments arranged by size: 904 754 353 290 273 198 173 154 120 103 93 38 EcoO109I rG'GnC_Cy Cuts at: 0 435 1483 3453 Size: 435 1048 1970 Fragments arranged by size: 1970 1048 435 EcoRI G'AATT_C Cuts at: 0 951 979 3278 3453 Size: 951 28 2299 175 Fragments arranged by size: 2299 951 175 28 EcoRV GAT'ATC Cuts at: 0 1518 2367 3375 3453 Size: 1518 849 1008 78 Fragments arranged by size: 1518 1008 849 78 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 417 1412 3453 Size: 417 995 2041 Fragments arranged by size: 2041 995 417 FspI TGC'GCA Cuts at: 0 2200 3453 Size: 2200 1253 FspAI rTGC'GCAy Cuts at: 0 2200 3453 Size: 2200 1253 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 88 120 232 264 414 441 446 473 Size: 88 32 112 32 150 27 5 27 Cuts at: 473 592 624 1282 1314 2351 2383 2520 2552 Size: 119 32 658 32 1037 32 137 32 Cuts at: 2552 3205 3237 3453 Size: 653 32 216 Fragments arranged by size: 1037 658 653 216 150 137 119 112 88 32 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 0 349 2665 3453 Size: 349 2316 788 Fragments arranged by size: 2316 788 349 HindIII A'AGCT_T Cuts at: 0 132 1389 1535 1778 2069 3453 Size: 132 1257 146 243 291 1384 Fragments arranged by size: 1384 1257 291 243 146 132 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2192 3367 3453 Size: 2192 1175 86 Fragments arranged by size: 2192 1175 86 MscI TGG'CCA Cuts at: 0 1799 3453 Size: 1799 1654 MslI CAynn'nnrTG Cuts at: 0 2456 3453 Size: 2456 997 MspA1I CmG'CkG Cuts at: 0 684 1828 3453 Size: 684 1144 1625 Fragments arranged by size: 1625 1144 684 NaeI GCC'GGC Cuts at: 0 2092 3364 3453 Size: 2092 1272 89 Fragments arranged by size: 2092 1272 89 NcoI C'CATG_G Cuts at: 0 704 1523 3453 Size: 704 819 1930 Fragments arranged by size: 1930 819 704 NgoMIV G'CCGG_C Cuts at: 0 2090 3362 3453 Size: 2090 1272 91 Fragments arranged by size: 2090 1272 91 NspI r_CATG'y Cuts at: 0 3436 3448 3453 Size: 3436 12 5 Fragments arranged by size: 3436 12 5 PciI A'CATG_T Cuts at: 0 3444 3453 Size: 3444 9 PflMI CCAn_nnn'nTGG Cuts at: 0 2953 3349 3453 Size: 2953 396 104 Fragments arranged by size: 2953 396 104 PfoI T'CCnGG_A Cuts at: 0 215 2373 3453 Size: 215 2158 1080 Fragments arranged by size: 2158 1080 215 PpuMI rG'GwC_Cy Cuts at: 0 435 3453 Size: 435 3018 PshAI GACnn'nnGTC Cuts at: 0 2660 3453 Size: 2660 793 PsiI TTA'TAA Cuts at: 0 3403 3453 Size: 3403 50 PspOMI G'GGCC_C Cuts at: 0 1482 3453 Size: 1482 1971 PspXI vC'TCGA_Gb Cuts at: 0 929 3453 Size: 929 2524 PstI C_TGCA'G Cuts at: 0 797 820 3453 Size: 797 23 2633 Fragments arranged by size: 2633 797 23 PvuII CAG'CTG Cuts at: 0 684 1828 3453 Size: 684 1144 1625 Fragments arranged by size: 1625 1144 684 SacI G_AGCT'C Cuts at: 0 936 1151 1300 3453 Size: 936 215 149 2153 Fragments arranged by size: 2153 936 215 149 SalI G'TCGA_C Cuts at: 0 347 3453 Size: 347 3106 SapI GCTCTTCn'nnn_ Cuts at: 0 572 3453 Size: 572 2881 SfcI C'TryA_G Cuts at: 0 793 816 1017 3453 Size: 793 23 201 2436 Fragments arranged by size: 2436 793 201 23 SmlI C'TyrA_G Cuts at: 0 609 929 1076 1532 2318 3453 Size: 609 320 147 456 786 1135 Fragments arranged by size: 1135 786 609 456 320 147 SspI AAT'ATT Cuts at: 0 1461 3358 3453 Size: 1461 1897 95 Fragments arranged by size: 1897 1461 95 StuI AGG'CCT Cuts at: 0 2256 3453 Size: 2256 1197 StyI C'CwwG_G Cuts at: 0 704 1523 1634 3453 Size: 704 819 111 1819 Fragments arranged by size: 1819 819 704 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2369 3056 3453 Size: 2369 687 397 Fragments arranged by size: 2369 687 397 TatI w'GTAC_w Cuts at: 0 521 547 809 3453 Size: 521 26 262 2644 Fragments arranged by size: 2644 521 262 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1048 1080 3453 Size: 1048 32 2373 Fragments arranged by size: 2373 1048 32 Tth111I GACn'n_nGTC Cuts at: 0 2532 3453 Size: 2532 921 XbaI T'CTAG_A Cuts at: 0 936 3453 Size: 936 2517 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 468 2547 3453 Size: 468 2079 906 Fragments arranged by size: 2079 906 468 XhoI C'TCGA_G Cuts at: 0 929 3453 Size: 929 2524 Enzymes that do cut: AccI AcuI AflIII AhdI ApaI ApaLI ApoI AseI AvaI BanII BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstYI BtgI BtsI CspCI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PciI PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PstI PvuII SacI SalI SapI SfcI SmlI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AleI AlfI AloI AlwNI AscI AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI BfrBI BmtI BsaAI BsaHI BseRI BsiWI BsmI BspEI BspHI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI EciI EcoNI FseI HaeII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PsrI PvuI RsrII SacII SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI XmaI XmnI ZraI