################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspHI AspI AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiHKAI BsiWI BsmFI BspDI BspEI BspHI BssHII BssSI BstBI BstEII BstPI BstUI BstXI CelII ClaI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FseI HaeII HgaI Hin1I HpaI KasI KpnI KspI MaeI MaeII MamI MluI MluNI MroI MscI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AflIII 179 ACATGT A^C[GA][CT]GT AlwNI 358 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 568 CCTGAGG CC^T[ACGT]AGG AseI 385 ATTAAT AT^TAAT AsnI 385 ATTAAT AT^TAAT AvaI 1415 CTCGAG C^[CT]CG[GA]G AviII 74 TGCGCA TGC^GCA BbsI 876 GTCTTC GAAGAC(2/6) BpuAI 876 GTCTTC GAAGAC(2/6) BsaI 441 GAGACC GGTCTC(1/5) BsaWI 1100 TCCGGT [AT]^CCGG[AT] BsgI 800 GTGCAG GTGCAG(16/14) BsmI 371 GCATTC GAATGC(1/-1) BsoBI 1415 CTCGAG C^[CT]CG[GA]G Bsp1407I 352 TGTACA T^GTACA BspLU11I 179 ACATGT A^CATGT BspMI 1120 ACCTGC ACCTGC(4/8) BsrDI 1036 GCAATG GCAATG(2/0) BsrFI 518 ACCGGC [GA]^CCGG[CT] BsrGI 352 TGTACA T^GTACA BstAPI 1124 GCATCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 996 GGATG GGATG(2/0) BstYI 359 AGATCC [GA]^GATC[CT] Bsu36I 568 CCTGAGG CC^T[ACGT]AGG Cfr10I 518 ACCGGC [GA]^CCGG[CT] CpoI 680 CGGACCG CG^G[AT]CCG DraII 1377 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 136 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 898 CGGCCA [CT]^GGCC[GA] Eco81I 568 CCTGAGG CC^T[ACGT]AGG EcoO109I 1377 GGGGCCT [GA]G^G[ACGT]CC[CT] FokI 996 GGATG GGATG(9/13) FspI 74 TGCGCA TGC^GCA HincII 916 GTTGAC GT[CT]^[GA]AC HindII 916 GTTGAC GT[CT]^[GA]AC HindIII 122 AAGCTT A^AGCTT MfeI 22 CAATTG C^AATTG MflI 359 AGATCC [GA]^GATC[CT] MunI 22 CAATTG C^AATTG PaeR7I 1415 CTCGAG C^TCGAG PshBI 385 ATTAAT AT^TAAT RsrII 680 CGGACCG CG^G[AT]CCG SfcI 848 CTGTAG C^T[GA][CT]AG SmlI 1415 CTCGAG C^T[CT][GA]AG SphI 77 GCATGC GCATG^C SspBI 352 TGTACA T^GTACA TaqI 1416 TCGA T^CGA TthHB8I 1416 TCGA T^CGA XhoI 1415 CTCGAG C^TCGAG XhoII 359 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccI 1227 GTATAC GT^[AC][GT]AC 1364 GTATAC AvaII 681 GGACC G^G[AT]CC 1164 GGACC BbvCI 394 GCTGAGG CCTCAGC(-5/-2) 1426 GCTGAGG BglI 886 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 929 GCCCCTCCGGC BseRI 571 GAGGAG GAGGAG(10/8) 751 GAGGAG BsmBI 88 CGTCTC CGTCTC(1/5) 1114 CGTCTC BsrBI 480 CCGCTC CCGCTC(-3/-3) 1090 GAGCGG Bst1107I 1227 GTATAC GTA^TAC 1364 GTATAC BstZ17I 1227 GTATAC GTA^TAC 1364 GTATAC EcoRV 292 GATATC GAT^ATC 547 GATATC MseI 386 TTAA T^TAA 412 TTAA MslI 1288 CACTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1294 CATGTCTGTG MspA1I 311 CAGCTG C[AC]G^C[GT]G 1243 CAGCTG NcoI 818 CCATGG C^CATGG 840 CCATGG PvuII 311 CAGCTG CAG^CTG 1243 CAGCTG Tru9I 386 TTAA T^TAA 412 TTAA Tsp45I 1408 GTCAC ^GT[GC]AC 1437 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AfaI AluI AlwI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI TseI Tsp509I TspRI