(Linear) (Six-base) MAPSORT of: KIEE4120.seq Check: 9553 from: 1 to: 1509 KIEE4120.orf With 182 enzymes: SgfI * July 3, 2012 18:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1129 1509 Size: 1129 380 AccI GT'mk_AC Cuts at: 0 1228 1365 1509 Size: 1228 137 144 Fragments arranged by size: 1228 144 137 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 863 1509 Size: 24 839 646 Fragments arranged by size: 839 646 24 AflIII A'CryG_T Cuts at: 0 179 1509 Size: 179 1330 AlwNI CAG_nnn'CTG Cuts at: 0 363 1509 Size: 363 1146 AseI AT'TA_AT Cuts at: 0 386 1509 Size: 386 1123 AvaI C'yCGr_G Cuts at: 0 1415 1509 Size: 1415 94 BanII G_rGCy'C Cuts at: 0 135 629 1486 1509 Size: 135 494 857 23 Fragments arranged by size: 857 494 135 23 BbsI GAAGACnn'nnnn_ Cuts at: 0 869 1509 Size: 869 640 BbvCI CC'TCA_GC Cuts at: 0 395 1427 1509 Size: 395 1032 82 Fragments arranged by size: 1032 395 82 BglI GCCn_nnn'nGGC Cuts at: 0 892 935 1509 Size: 892 43 574 Fragments arranged by size: 892 574 43 BmrI ACTGGGnnnn_n' Cuts at: 0 1508 1509 Size: 1508 1 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 385 431 769 803 1267 1393 1509 Size: 385 46 338 34 464 126 116 Fragments arranged by size: 464 385 338 126 116 46 34 Bpu10I CC'TnA_GC Cuts at: 0 55 395 487 1004 1427 1509 Size: 55 340 92 517 423 82 Fragments arranged by size: 517 423 340 92 82 55 BsaI GGTCTCn'nnnn_ Cuts at: 0 435 1509 Size: 435 1074 BsaWI w'CCGG_w Cuts at: 0 1100 1509 Size: 1100 409 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 875 905 1375 1405 1446 1476 1509 Size: 875 30 470 30 41 30 33 Fragments arranged by size: 875 470 41 33 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 586 766 1509 Size: 586 180 743 Fragments arranged by size: 743 586 180 BseYI C'CCAG_C Cuts at: 0 1012 1509 Size: 1012 497 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 821 1509 Size: 821 688 BsmI GAATG_Cn' Cuts at: 0 371 1509 Size: 371 1138 BsmBI CGTCTCn'nnnn_ Cuts at: 0 94 1120 1509 Size: 94 1026 389 Fragments arranged by size: 1026 389 94 Bsp1286I G_dGCh'C Cuts at: 0 135 629 1486 1509 Size: 135 494 857 23 Fragments arranged by size: 857 494 135 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1129 1509 Size: 1129 380 BsrBI CCG'CTC Cuts at: 0 482 1092 1509 Size: 482 610 417 Fragments arranged by size: 610 482 417 BsrDI GCAATG_nn' Cuts at: 0 1043 1509 Size: 1043 466 BsrFI r'CCGG_y Cuts at: 0 518 1509 Size: 518 991 BsrGI T'GTAC_A Cuts at: 0 352 1509 Size: 352 1157 BstAPI GCAn_nnn'nTGC Cuts at: 0 1130 1509 Size: 1130 379 BstYI r'GATC_y Cuts at: 0 359 1509 Size: 359 1150 BstZ17I GTA'TAC Cuts at: 0 1229 1366 1509 Size: 1229 137 143 Fragments arranged by size: 1229 143 137 Bsu36I CC'TnA_GG Cuts at: 0 569 1509 Size: 569 940 BtgI C'CryG_G Cuts at: 0 684 818 840 1509 Size: 684 134 22 669 Fragments arranged by size: 684 669 134 22 BtsI GCAGTG_nn' Cuts at: 0 915 945 1509 Size: 915 30 564 Fragments arranged by size: 915 564 30 DraIII CAC_nnn'GTG Cuts at: 0 141 1509 Size: 141 1368 EaeI y'GGCC_r Cuts at: 0 898 1509 Size: 898 611 EarI CTCTTCn'nnn_ Cuts at: 0 1022 1427 1445 1509 Size: 1022 405 18 64 Fragments arranged by size: 1022 405 64 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 285 694 1509 Size: 285 409 815 Fragments arranged by size: 815 409 285 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 385 431 769 803 863 1267 1393 Size: 24 361 46 338 34 60 404 126 Cuts at: 1393 1509 Size: 116 Fragments arranged by size: 404 361 338 126 116 60 46 34 24 EcoO109I rG'GnC_Cy Cuts at: 0 1378 1509 Size: 1378 131 EcoRV GAT'ATC Cuts at: 0 294 549 1509 Size: 294 255 960 Fragments arranged by size: 960 294 255 FspI TGC'GCA Cuts at: 0 76 1509 Size: 76 1433 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 166 198 417 449 1420 1446 1452 1478 Size: 166 32 219 32 971 26 6 26 Cuts at: 1478 1509 Size: 31 Fragments arranged by size: 971 219 166 32 32 31 26 26 6 HincII GTy'rAC Cuts at: 0 918 1509 Size: 918 591 HindIII A'AGCT_T Cuts at: 0 122 1509 Size: 122 1387 MfeI C'AATT_G Cuts at: 0 22 1509 Size: 22 1487 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 673 1509 Size: 673 836 MslI CAynn'nnrTG Cuts at: 0 1292 1298 1509 Size: 1292 6 211 Fragments arranged by size: 1292 211 6 MspA1I CmG'CkG Cuts at: 0 313 1245 1509 Size: 313 932 264 Fragments arranged by size: 932 313 264 NcoI C'CATG_G Cuts at: 0 818 840 1509 Size: 818 22 669 Fragments arranged by size: 818 669 22 NspI r_CATG'y Cuts at: 0 81 183 1176 1215 1509 Size: 81 102 993 39 294 Fragments arranged by size: 993 294 102 81 39 PciI A'CATG_T Cuts at: 0 179 1509 Size: 179 1330 PfoI T'CCnGG_A Cuts at: 0 170 362 1509 Size: 170 192 1147 Fragments arranged by size: 1147 192 170 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1081 1113 1509 Size: 1081 32 396 Fragments arranged by size: 1081 396 32 PvuII CAG'CTG Cuts at: 0 313 1245 1509 Size: 313 932 264 Fragments arranged by size: 932 313 264 RsrII CG'GwC_CG Cuts at: 0 681 1509 Size: 681 828 SfcI C'TryA_G Cuts at: 0 848 1509 Size: 848 661 SmlI C'TyrA_G Cuts at: 0 1415 1509 Size: 1415 94 SphI G_CATG'C Cuts at: 0 81 1509 Size: 81 1428 StyI C'CwwG_G Cuts at: 0 818 840 887 1509 Size: 818 22 47 622 Fragments arranged by size: 818 622 47 22 TatI w'GTAC_w Cuts at: 0 199 352 726 1509 Size: 199 153 374 783 Fragments arranged by size: 783 374 199 153 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 875 907 1375 1407 1509 Size: 875 32 468 32 102 Fragments arranged by size: 875 468 102 32 32 XhoI C'TCGA_G Cuts at: 0 1415 1509 Size: 1415 94 Enzymes that do cut: AarI AccI AcuI AflIII AlwNI AseI AvaI BanII BbsI BbvCI BglI BmrI BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstYI BstZ17I Bsu36I BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRV FspI Hin4I HincII HindIII MfeI MmeI MslI MspA1I NcoI NspI PciI PfoI PpiI PvuII RsrII SfcI SmlI SphI StyI TatI TstI XhoI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BspEI BspHI BssHII BssSI BstBI BstEII BstXI BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI FalI FseI FspAI HaeII HpaI KasI KpnI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI XmaI XmnI ZraI