(Circular) (Six-base) MAPSORT of: pFN21AE4089.seq Check: 9043 from: 1 to: 6064 pFN21AE4089 6064 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2112 2112 Size: 6064 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3407 1937 Size: 1470 4594 Acc65I G'GTAC_C Cuts at: 3385 3385 Size: 6064 AclI AA'CG_TT Cuts at: 1563 4191 4564 1563 Size: 2628 373 3063 Fragments arranged by size: 3063 2628 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3033 4127 5511 5779 1632 Size: 1401 1094 1384 268 1917 Fragments arranged by size: 1917 1401 1384 1094 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5867 AflIII A'CryG_T Cuts at: 1203 2677 4963 5940 1203 Size: 1474 2286 977 1327 Fragments arranged by size: 2286 1474 1327 977 AgeI A'CCGG_T Cuts at: 4895 4895 Size: 6064 AhdI GACnn_n'nnGTC Cuts at: 4793 4793 Size: 6064 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6064 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6064 AlwNI CAG_nnn'CTG Cuts at: 3244 3807 5379 3244 Size: 563 1572 3929 Fragments arranged by size: 3929 1572 563 ApaI G_GGCC'C Cuts at: 2146 2774 2929 3100 2146 Size: 628 155 171 5110 Fragments arranged by size: 5110 628 171 155 ApaLI G'TGCA_C Cuts at: 4121 5277 4121 Size: 1156 4908 ApoI r'AATT_y Cuts at: 1493 3373 3599 3751 5860 1493 Size: 1880 226 152 2109 1697 Fragments arranged by size: 2109 1880 1697 226 152 AscI GG'CGCG_CC Cuts at: 2804 2804 Size: 6064 AseI AT'TA_AT Cuts at: 160 1032 4618 160 Size: 872 3586 1606 Fragments arranged by size: 3586 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 2139 2364 2460 2561 2835 3093 3389 Size: 73 196 225 96 101 274 258 296 Cuts at: 3389 1870 Size: 4545 Fragments arranged by size: 4545 296 274 258 225 196 101 96 73 BamHI G'GATC_C Cuts at: 3394 3761 3394 Size: 367 5697 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2124 2341 2702 3293 3385 Size: 325 217 627 337 217 361 591 92 Cuts at: 3385 4840 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 591 361 337 325 217 217 92 BanII G_rGCy'C Cuts at: 1391 2146 2467 2774 2929 3100 3383 1391 Size: 755 321 307 155 171 283 4072 Fragments arranged by size: 4072 755 321 307 283 171 155 BbeI G_GCGC'C Cuts at: 2706 2706 Size: 6064 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5093 BbvCI CC'TCA_GC Cuts at: 2934 2934 Size: 6064 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2524 2558 4255 4289 1903 Size: 34 587 34 1697 34 3678 Fragments arranged by size: 3678 1697 587 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3956 5166 3956 Size: 1210 4854 BclI T'GATC_A Cuts at: 1548 1548 Size: 6064 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2294 2840 4675 Size: 107 122 71 631 605 622 546 1835 Cuts at: 4675 136 Size: 1525 Fragments arranged by size: 1835 1525 631 622 605 546 122 107 71 BglII A'GATC_T Cuts at: 6059 6059 Size: 6064 BlpI GC'TnA_GC Cuts at: 2409 3481 2409 Size: 1072 4992 Bme1580I G_kGCm'C Cuts at: 1790 2129 2146 2461 2534 2774 2929 3100 4125 Size: 339 17 315 73 240 155 171 1025 Cuts at: 4125 5281 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1025 339 315 240 171 155 73 17 BmgBI CAC'GTC Cuts at: 1318 2710 1318 Size: 1392 4672 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2461 3013 3795 Size: 416 53 83 399 24 1044 552 782 Cuts at: 3795 4753 442 Size: 958 1753 Fragments arranged by size: 1753 1044 958 782 552 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2690 2714 2837 2844 4724 1239 Size: 1451 24 123 7 1880 2579 Fragments arranged by size: 2579 1880 1451 123 24 7 Bpu10I CC'TnA_GC Cuts at: 2934 2934 Size: 6064 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4189 5054 5352 4189 Size: 865 298 4901 Fragments arranged by size: 4901 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2009 2169 3137 4727 882 Size: 617 510 160 968 1590 2219 Fragments arranged by size: 2219 1590 968 617 510 160 BsaAI yAC'GTr Cuts at: 493 1204 2650 3855 493 Size: 711 1446 1205 2702 Fragments arranged by size: 2702 1446 1205 711 BsaBI GATnn'nnATC Cuts at: 6058 6058 Size: 6064 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2639 2703 3871 4253 Size: 53 83 186 936 1106 64 1168 382 Cuts at: 4253 4888 275 Size: 635 1451 Fragments arranged by size: 1451 1168 1106 936 635 382 186 83 64 53 BsaWI w'CCGG_w Cuts at: 4497 4895 5169 5316 5727 4497 Size: 398 274 147 411 4834 Fragments arranged by size: 4834 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2592 2622 1178 Size: 30 165 30 1189 30 4620 Fragments arranged by size: 4620 1189 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2540 3311 1186 Size: 837 517 771 3939 Fragments arranged by size: 3939 837 771 517 BseYI C'CCAG_C Cuts at: 2103 2179 2759 5267 5763 2103 Size: 76 580 2508 496 2404 Fragments arranged by size: 2508 2404 580 496 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2536 2630 2852 1163 Size: 573 800 94 222 4375 Fragments arranged by size: 4375 800 573 222 94 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2844 3514 4275 5303 1540 Size: 275 186 843 670 761 1028 2301 Fragments arranged by size: 2301 1028 843 761 670 275 186 BsiHKAI G_wGCw'C Cuts at: 2386 3165 3186 4125 4210 5281 2386 Size: 779 21 939 85 1071 3169 Fragments arranged by size: 3169 1071 939 779 85 21 BsmI GAATG_Cn' Cuts at: 3575 3668 3575 Size: 93 5971 BsmBI CGTCTCn'nnnn_ Cuts at: 2630 2630 Size: 6064 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2146 2386 2461 2467 2534 2774 Size: 399 339 17 240 75 6 67 240 Cuts at: 2774 2929 3100 3165 3186 3383 4125 4210 5281 Size: 155 171 65 21 197 742 85 1071 Cuts at: 5281 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 339 240 240 197 171 155 85 75 67 65 21 17 6 BspEI T'CCGG_A Cuts at: 5727 5727 Size: 6064 BspHI T'CATG_A Cuts at: 3953 5683 3953 Size: 1730 4334 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2112 2209 2220 3342 3419 844 Size: 1268 97 11 1122 77 3489 Fragments arranged by size: 3489 1268 1122 97 77 11 BsrBI CCG'CTC Cuts at: 1394 1823 3319 3951 1394 Size: 429 1496 632 3507 Fragments arranged by size: 3507 1496 632 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4559 4733 65 Size: 1183 3311 174 1396 Fragments arranged by size: 3311 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 2808 3285 4708 4895 1700 Size: 110 275 723 477 1423 187 2869 Fragments arranged by size: 2869 1423 723 477 275 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6064 BssHII G'CGCG_C Cuts at: 1925 2660 2804 3262 1925 Size: 735 144 458 4727 Fragments arranged by size: 4727 735 458 144 BssSI C'ACGA_G Cuts at: 4124 5136 4124 Size: 1012 5052 BstAPI GCAn_nnn'nTGC Cuts at: 794 3055 794 Size: 2261 3803 BstBI TT'CG_AA Cuts at: 4868 4868 Size: 6064 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6064 BstXI CCAn_nnnn'nTGG Cuts at: 3174 3174 Size: 6064 BstYI r'GATC_y Cuts at: 1972 3394 3761 4146 4163 5615 5701 5713 6059 Size: 1422 367 385 17 1452 86 12 346 Cuts at: 6059 1972 Size: 1977 Fragments arranged by size: 1977 1452 1422 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3323 3323 Size: 6064 BtgI C'CryG_G Cuts at: 513 1065 2004 2964 513 Size: 552 939 960 3613 Fragments arranged by size: 3613 960 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2338 487 Size: 683 56 1112 4213 Fragments arranged by size: 4213 1112 683 56 BtsI GCAGTG_nn' Cuts at: 2114 2579 3029 3076 3308 3580 4374 4394 2114 Size: 465 450 47 232 272 794 20 3784 Fragments arranged by size: 3784 794 465 450 272 232 47 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6064 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 3368 3368 Size: 6064 DraIII CAC_nnn'GTG Cuts at: 2754 2754 Size: 6064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2183 5071 809 Size: 507 867 2888 1802 Fragments arranged by size: 2888 1802 867 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2841 2901 3511 Size: 54 1425 50 275 18 1011 60 610 Cuts at: 3511 4400 6029 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1011 889 610 275 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2841 3511 1537 Size: 275 1029 670 4090 Fragments arranged by size: 4090 1029 670 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2204 2238 3994 1353 Size: 851 34 1756 3423 Fragments arranged by size: 3423 1756 851 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 4651 5025 5171 387 Size: 1064 138 453 2609 374 146 1280 Fragments arranged by size: 2609 1280 1064 453 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2690 2714 2837 2844 3033 4127 4724 Size: 393 1058 24 123 7 189 1094 597 Cuts at: 4724 5511 5779 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1094 1058 787 597 393 268 189 123 24 7 EcoNI CCTnn'n_nnAGG Cuts at: 2060 2216 2060 Size: 156 5908 EcoO109I rG'GnC_Cy Cuts at: 2770 2896 2925 2938 3097 2770 Size: 126 29 13 159 5737 Fragments arranged by size: 5737 159 126 29 13 EcoRI G'AATT_C Cuts at: 3373 3751 3373 Size: 378 5686 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3128 3128 Size: 6064 FspI TGC'GCA Cuts at: 2720 4570 2720 Size: 1850 4214 HaeII r_GCGC'y Cuts at: 1678 2706 2857 5211 1678 Size: 1028 151 2354 2531 Fragments arranged by size: 2531 2354 1028 151 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2379 2411 4705 4737 4779 Size: 32 135 32 807 32 2294 32 42 Cuts at: 4779 4811 1373 Size: 32 2626 Fragments arranged by size: 2626 2294 807 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3408 3654 669 Size: 988 281 1470 246 3079 Fragments arranged by size: 3079 1470 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6064 HpaI GTT'AAC Cuts at: 3654 3654 Size: 6064 KasI G'GCGC_C Cuts at: 2702 2702 Size: 6064 KpnI G_GTAC'C Cuts at: 3389 3389 Size: 6064 MfeI C'AATT_G Cuts at: 3663 3663 Size: 6064 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5178 5362 1305 Size: 3873 184 2007 Fragments arranged by size: 3873 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4242 Fragments arranged by size: 4242 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2261 2963 4022 4381 4540 518 Size: 648 1095 702 1059 359 159 2042 Fragments arranged by size: 2042 1095 1059 702 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2183 2284 2866 3235 3282 3480 4157 5305 Size: 577 101 582 369 47 198 677 1148 Cuts at: 5305 5550 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 582 577 369 245 198 101 47 NaeI GCC'GGC Cuts at: 2810 3287 2810 Size: 477 5587 NarI GG'CG_CC Cuts at: 2703 2703 Size: 6064 NcoI C'CATG_G Cuts at: 513 1065 2004 2964 513 Size: 552 939 960 3613 Fragments arranged by size: 3613 960 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6064 NgoMIV G'CCGG_C Cuts at: 2808 3285 2808 Size: 477 5587 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 2681 3422 4967 1132 Size: 97 1452 741 1545 2229 Fragments arranged by size: 2229 1545 1452 741 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6064 PciI A'CATG_T Cuts at: 2677 4963 2677 Size: 2286 3778 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6064 PfoI T'CCnGG_A Cuts at: 2814 2988 2814 Size: 174 5890 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PmlI CAC'GTG Cuts at: 2650 2650 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4165 5646 5678 4133 Size: 32 1481 32 4519 Fragments arranged by size: 4519 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2938 2938 Size: 6064 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6064 PsiI TTA'TAA Cuts at: 3634 3634 Size: 6064 PspOMI G'GGCC_C Cuts at: 2142 2770 2925 3096 2142 Size: 628 155 171 5110 Fragments arranged by size: 5110 628 171 155 PstI C_TGCA'G Cuts at: 830 2061 2217 2786 2982 3240 3416 830 Size: 1231 156 569 196 258 176 3478 Fragments arranged by size: 3478 1231 569 258 196 176 156 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 PvuII CAG'CTG Cuts at: 2183 2284 3235 2183 Size: 101 951 5012 Fragments arranged by size: 5012 951 101 SalI G'TCGA_C Cuts at: 1936 3406 1936 Size: 1470 4594 SbfI CC_TGCA'GG Cuts at: 3416 3416 Size: 6064 ScaI AGT'ACT Cuts at: 4312 6024 4312 Size: 1712 4352 SfcI C'TryA_G Cuts at: 826 1045 2057 2213 2358 2782 2978 3196 3236 Size: 219 1012 156 145 424 196 218 40 Cuts at: 3236 3412 4547 5228 5419 826 Size: 176 1135 681 191 1471 Fragments arranged by size: 1471 1135 1012 681 424 219 218 196 191 176 156 145 40 SfoI GGC'GCC Cuts at: 2704 2704 Size: 6064 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6064 SmaI CCC'GGG Cuts at: 1872 2141 2837 3391 1872 Size: 269 696 554 4545 Fragments arranged by size: 4545 696 554 269 SmlI C'TyrA_G Cuts at: 820 1017 1943 4168 5069 5331 820 Size: 197 926 2225 901 262 1553 Fragments arranged by size: 2225 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3855 493 Size: 3362 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6064 SphI G_CATG'C Cuts at: 1132 3422 1132 Size: 2290 3774 SrfI GCCC'GGGC Cuts at: 2837 2837 Size: 6064 SspI AAT'ATT Cuts at: 5 52 3988 5 Size: 47 3936 2081 Fragments arranged by size: 3936 2081 47 StuI AGG'CCT Cuts at: 1517 2158 2453 1517 Size: 641 295 5128 Fragments arranged by size: 5128 641 295 StyI C'CwwG_G Cuts at: 513 1065 2004 2505 2964 3227 3503 513 Size: 552 939 501 459 263 276 3074 Fragments arranged by size: 3074 939 552 501 459 276 263 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2302 3139 4091 4108 4261 4446 4891 Size: 1255 722 837 952 17 153 185 445 Cuts at: 4891 325 Size: 1498 Fragments arranged by size: 1498 1255 952 837 722 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4310 6022 96 Size: 274 80 33 51 1443 2333 1712 138 Fragments arranged by size: 2333 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2592 2624 6024 6056 2592 Size: 32 3400 32 2600 Fragments arranged by size: 3400 2600 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6064 XbaI T'CTAG_A Cuts at: 3400 3400 Size: 6064 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2260 2587 2260 Size: 327 5737 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6064 XmaI C'CCGG_G Cuts at: 1870 2139 2835 3389 1870 Size: 269 696 554 4545 Fragments arranged by size: 4545 696 554 269 XmnI GAAnn'nnTTC Cuts at: 4193 4193 Size: 6064 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SwaI