(Linear) (Six-base) MAPSORT of: KIEE4089.seq Check: 1216 from: 1 to: 1359 KIEE4089.orf With 182 enzymes: SgfI * July 3, 2012 18:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 107 1359 Size: 107 1252 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1028 1359 Size: 1028 331 AflIII A'CryG_T Cuts at: 0 672 1359 Size: 672 687 AlwNI CAG_nnn'CTG Cuts at: 0 1239 1359 Size: 1239 120 ApaI G_GGCC'C Cuts at: 0 141 769 924 1095 1359 Size: 141 628 155 171 264 Fragments arranged by size: 628 264 171 155 141 AscI GG'CGCG_CC Cuts at: 0 799 1359 Size: 799 560 AvaI C'yCGr_G Cuts at: 0 134 359 455 556 830 1088 1359 Size: 134 225 96 101 274 258 271 Fragments arranged by size: 274 271 258 225 134 101 96 BanI G'GyrC_C Cuts at: 0 119 336 697 1288 1359 Size: 119 217 361 591 71 Fragments arranged by size: 591 361 217 119 71 BanII G_rGCy'C Cuts at: 0 141 462 769 924 1095 1359 Size: 141 321 307 155 171 264 Fragments arranged by size: 321 307 264 171 155 141 BbeI G_GCGC'C Cuts at: 0 701 1359 Size: 701 658 BbvCI CC'TCA_GC Cuts at: 0 929 1359 Size: 929 430 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 519 553 1359 Size: 519 34 806 Fragments arranged by size: 806 519 34 BglI GCCn_nnn'nGGC Cuts at: 0 289 835 1359 Size: 289 546 524 Fragments arranged by size: 546 524 289 BlpI GC'TnA_GC Cuts at: 0 404 1359 Size: 404 955 Bme1580I G_kGCm'C Cuts at: 0 124 141 456 529 769 924 1095 1359 Size: 124 17 315 73 240 155 171 264 Fragments arranged by size: 315 264 240 171 155 124 73 17 BmgBI CAC'GTC Cuts at: 0 705 1359 Size: 705 654 BmrI ACTGGGnnnn_n' Cuts at: 0 456 1008 1359 Size: 456 552 351 Fragments arranged by size: 552 456 351 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 685 709 832 839 1359 Size: 685 24 123 7 520 Fragments arranged by size: 685 520 123 24 7 Bpu10I CC'TnA_GC Cuts at: 0 929 1359 Size: 929 430 BsaI GGTCTCn'nnnn_ Cuts at: 0 4 164 1132 1359 Size: 4 160 968 227 Fragments arranged by size: 968 227 160 4 BsaAI yAC'GTr Cuts at: 0 645 1359 Size: 645 714 BsaHI Gr'CG_yC Cuts at: 0 634 698 1359 Size: 634 64 661 Fragments arranged by size: 661 634 64 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 587 617 1359 Size: 587 30 742 Fragments arranged by size: 742 587 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 18 535 1306 1359 Size: 18 517 771 53 Fragments arranged by size: 771 517 53 18 BseYI C'CCAG_C Cuts at: 0 98 174 754 1359 Size: 98 76 580 605 Fragments arranged by size: 605 580 98 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 531 625 847 1359 Size: 531 94 222 512 Fragments arranged by size: 531 512 222 94 BsiEI CG_ry'CG Cuts at: 0 839 1359 Size: 839 520 BsiHKAI G_wGCw'C Cuts at: 0 381 1160 1181 1359 Size: 381 779 21 178 Fragments arranged by size: 779 381 178 21 BsmBI CGTCTCn'nnnn_ Cuts at: 0 625 1359 Size: 625 734 Bsp1286I G_dGCh'C Cuts at: 0 124 141 381 456 462 529 769 924 Size: 124 17 240 75 6 67 240 155 Cuts at: 924 1095 1160 1181 1359 Size: 171 65 21 178 Fragments arranged by size: 240 240 178 171 155 124 75 67 65 21 17 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 107 204 215 1337 1359 Size: 107 97 11 1122 22 Fragments arranged by size: 1122 107 97 22 11 BsrBI CCG'CTC Cuts at: 0 1314 1359 Size: 1314 45 BsrFI r'CCGG_y Cuts at: 0 80 803 1280 1359 Size: 80 723 477 79 Fragments arranged by size: 723 477 80 79 BssHII G'CGCG_C Cuts at: 0 655 799 1257 1359 Size: 655 144 458 102 Fragments arranged by size: 655 458 144 102 BstAPI GCAn_nnn'nTGC Cuts at: 0 1050 1359 Size: 1050 309 BstXI CCAn_nnnn'nTGG Cuts at: 0 1169 1359 Size: 1169 190 Bsu36I CC'TnA_GG Cuts at: 0 1318 1359 Size: 1318 41 BtgI C'CryG_G Cuts at: 0 959 1359 Size: 959 400 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 333 1359 Size: 333 1026 BtsI GCAGTG_nn' Cuts at: 0 109 574 1024 1071 1303 1359 Size: 109 465 450 47 232 56 Fragments arranged by size: 465 450 232 109 56 47 DraIII CAC_nnn'GTG Cuts at: 0 749 1359 Size: 749 610 DrdI GACnn_nn'nnGTC Cuts at: 0 178 1359 Size: 178 1181 EaeI y'GGCC_r Cuts at: 0 836 896 1359 Size: 836 60 463 Fragments arranged by size: 836 463 60 EagI C'GGCC_G Cuts at: 0 836 1359 Size: 836 523 EarI CTCTTCn'nnn_ Cuts at: 0 199 233 1359 Size: 199 34 1126 Fragments arranged by size: 1126 199 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 37 1359 Size: 37 1322 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 685 709 832 839 1028 1359 Size: 685 24 123 7 189 331 Fragments arranged by size: 685 331 189 123 24 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 55 211 1359 Size: 55 156 1148 Fragments arranged by size: 1148 156 55 EcoO109I rG'GnC_Cy Cuts at: 0 765 891 920 933 1092 1359 Size: 765 126 29 13 159 267 Fragments arranged by size: 765 267 159 126 29 13 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1123 1359 Size: 1123 236 FspI TGC'GCA Cuts at: 0 715 1359 Size: 715 644 HaeII r_GCGC'y Cuts at: 0 701 852 1359 Size: 701 151 507 Fragments arranged by size: 701 507 151 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 374 406 1359 Size: 374 32 953 Fragments arranged by size: 953 374 32 KasI G'GCGC_C Cuts at: 0 697 1359 Size: 697 662 MslI CAynn'nnrTG Cuts at: 0 256 958 1359 Size: 256 702 401 Fragments arranged by size: 702 401 256 MspA1I CmG'CkG Cuts at: 0 178 279 861 1230 1277 1359 Size: 178 101 582 369 47 82 Fragments arranged by size: 582 369 178 101 82 47 NaeI GCC'GGC Cuts at: 0 805 1282 1359 Size: 805 477 77 Fragments arranged by size: 805 477 77 NarI GG'CG_CC Cuts at: 0 698 1359 Size: 698 661 NcoI C'CATG_G Cuts at: 0 959 1359 Size: 959 400 NgoMIV G'CCGG_C Cuts at: 0 803 1280 1359 Size: 803 477 79 Fragments arranged by size: 803 477 79 NspI r_CATG'y Cuts at: 0 676 1359 Size: 676 683 PciI A'CATG_T Cuts at: 0 672 1359 Size: 672 687 PfoI T'CCnGG_A Cuts at: 0 809 983 1359 Size: 809 174 376 Fragments arranged by size: 809 376 174 PmlI CAC'GTG Cuts at: 0 645 1359 Size: 645 714 PpuMI rG'GwC_Cy Cuts at: 0 933 1359 Size: 933 426 PspOMI G'GGCC_C Cuts at: 0 137 765 920 1091 1359 Size: 137 628 155 171 268 Fragments arranged by size: 628 268 171 155 137 PstI C_TGCA'G Cuts at: 0 56 212 781 977 1235 1359 Size: 56 156 569 196 258 124 Fragments arranged by size: 569 258 196 156 124 56 PvuII CAG'CTG Cuts at: 0 178 279 1230 1359 Size: 178 101 951 129 Fragments arranged by size: 951 178 129 101 SfcI C'TryA_G Cuts at: 0 52 208 353 777 973 1191 1231 1359 Size: 52 156 145 424 196 218 40 128 Fragments arranged by size: 424 218 196 156 145 128 52 40 SfoI GGC'GCC Cuts at: 0 699 1359 Size: 699 660 SmaI CCC'GGG Cuts at: 0 136 832 1359 Size: 136 696 527 Fragments arranged by size: 696 527 136 SrfI GCCC'GGGC Cuts at: 0 832 1359 Size: 832 527 StuI AGG'CCT Cuts at: 0 153 448 1359 Size: 153 295 911 Fragments arranged by size: 911 295 153 StyI C'CwwG_G Cuts at: 0 500 959 1222 1359 Size: 500 459 263 137 Fragments arranged by size: 500 459 263 137 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 297 1134 1359 Size: 297 837 225 Fragments arranged by size: 837 297 225 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 587 619 1359 Size: 587 32 740 Fragments arranged by size: 740 587 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 255 582 1359 Size: 255 327 777 Fragments arranged by size: 777 327 255 XmaI C'CCGG_G Cuts at: 0 134 830 1359 Size: 134 696 529 Fragments arranged by size: 696 529 134 Enzymes that do cut: AarI AcuI AflIII AlwNI ApaI AscI AvaI BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I KasI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmlI PpuMI PspOMI PstI PvuII SfcI SfoI SmaI SrfI StuI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglII BmtI BplI BpuEI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstYI BstZ17I ClaI CspCI DraI EcoICRI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI