(Circular) (Six-base) MAPSORT of: pFN21AB7312.seq Check: 4775 from: 1 to: 7882 pFN21AB7312 7882 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4516 4516 Size: 7882 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4620 5692 278 Size: 53 83 186 936 3084 1072 2468 Fragments arranged by size: 3084 2468 1072 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4044 4620 5225 1937 Size: 2107 576 605 4594 Fragments arranged by size: 4594 2107 605 576 Acc65I G'GTAC_C Cuts at: 5203 5203 Size: 7882 AclI AA'CG_TT Cuts at: 1563 6009 6382 1563 Size: 4446 373 3063 Fragments arranged by size: 4446 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3587 5945 7329 7597 1632 Size: 1955 2358 1384 268 1917 Fragments arranged by size: 2358 1955 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7685 AflIII A'CryG_T Cuts at: 1203 6781 7758 1203 Size: 5578 977 1327 Fragments arranged by size: 5578 1327 977 AgeI A'CCGG_T Cuts at: 6713 6713 Size: 7882 AhdI GACnn_n'nnGTC Cuts at: 3176 6611 3176 Size: 3435 4447 AleI CACnn'nnGTG Cuts at: 1166 2396 2685 3653 1166 Size: 1230 289 968 5395 Fragments arranged by size: 5395 1230 968 289 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4994 1184 Size: 3810 4072 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2465 2497 2465 Size: 32 7850 AlwNI CAG_nnn'CTG Cuts at: 2305 3073 5625 7197 2305 Size: 768 2552 1572 2990 Fragments arranged by size: 2990 2552 1572 768 ApaI G_GGCC'C Cuts at: 2824 3673 4073 2824 Size: 849 400 6633 Fragments arranged by size: 6633 849 400 ApaLI G'TGCA_C Cuts at: 5939 7095 5939 Size: 1156 6726 ApoI r'AATT_y Cuts at: 1493 5191 5417 5569 7678 1493 Size: 3698 226 152 2109 1697 Fragments arranged by size: 3698 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6436 160 Size: 872 5404 1606 Fragments arranged by size: 5404 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7882 AvaI C'yCGr_G Cuts at: 1870 1943 2635 3270 3510 3929 4297 4814 5020 Size: 73 692 635 240 419 368 517 206 Cuts at: 5020 5136 5207 1870 Size: 116 71 4545 Fragments arranged by size: 4545 692 635 517 419 368 240 206 116 73 71 AvrII C'CTAG_G Cuts at: 4651 4651 Size: 7882 BamHI G'GATC_C Cuts at: 2343 3294 4194 5212 5579 2343 Size: 951 900 1018 367 4646 Fragments arranged by size: 4646 1018 951 900 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3547 4462 4545 5203 6658 Size: 325 217 627 1760 915 83 658 1455 Cuts at: 6658 618 Size: 1842 Fragments arranged by size: 1842 1760 1455 915 658 627 325 217 83 BanII G_rGCy'C Cuts at: 1391 2013 2412 2824 3192 3395 3673 3713 3936 Size: 622 399 412 368 203 278 40 223 Cuts at: 3936 4073 4821 4871 5058 5201 1391 Size: 137 748 50 187 143 4072 Fragments arranged by size: 4072 748 622 412 399 368 278 223 203 187 143 137 50 40 BbeI G_GCGC'C Cuts at: 4466 4549 4466 Size: 83 7799 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6911 BbvCI CC'TCA_GC Cuts at: 2792 4928 2792 Size: 2136 5746 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6073 6107 1903 Size: 34 4136 34 3678 Fragments arranged by size: 4136 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5774 6984 5774 Size: 1210 6672 BclI T'GATC_A Cuts at: 1548 1548 Size: 7882 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4424 6493 136 Size: 107 122 71 631 605 2752 2069 1525 Fragments arranged by size: 2752 2069 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7877 7877 Size: 7882 BlpI GC'TnA_GC Cuts at: 2306 2406 5299 2306 Size: 100 2893 4889 Fragments arranged by size: 4889 2893 100 Bme1580I G_kGCm'C Cuts at: 1790 2040 2824 3552 3673 3683 4073 5943 7099 Size: 250 784 728 121 10 390 1870 1156 Cuts at: 7099 1790 Size: 2573 Fragments arranged by size: 2573 1870 1156 784 728 390 250 121 10 BmgBI CAC'GTC Cuts at: 1318 2881 4750 1318 Size: 1563 1869 4450 Fragments arranged by size: 4450 1869 1563 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3460 4952 5088 Size: 416 53 83 399 24 2043 1492 136 Cuts at: 5088 5613 6571 442 Size: 525 958 1753 Fragments arranged by size: 2043 1753 1492 958 525 416 399 136 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7882 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2560 3208 2560 Size: 648 7234 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2282 2285 2339 2614 2912 3320 3812 4220 Size: 1043 3 54 275 298 408 492 408 Cuts at: 4220 4709 4781 6542 1239 Size: 489 72 1761 2579 Fragments arranged by size: 2579 1761 1043 492 489 408 408 298 275 72 54 3 Bpu10I CC'TnA_GC Cuts at: 2792 3465 4143 4667 4928 2792 Size: 673 678 524 261 5746 Fragments arranged by size: 5746 678 673 524 261 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2990 6007 6872 7170 2990 Size: 3017 865 298 3702 Fragments arranged by size: 3702 3017 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2222 6545 882 Size: 617 723 4323 2219 Fragments arranged by size: 4323 2219 723 617 BsaAI yAC'GTr Cuts at: 493 1204 2173 2776 3287 5673 493 Size: 711 969 603 511 2386 2702 Fragments arranged by size: 2702 2386 969 711 603 511 BsaBI GATnn'nnATC Cuts at: 7876 7876 Size: 7882 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2255 3253 3947 4403 Size: 53 83 186 936 722 998 694 456 Cuts at: 4403 4416 4463 4546 4617 5689 6071 6706 275 Size: 13 47 83 71 1072 382 635 1451 Fragments arranged by size: 1451 1072 998 936 722 694 635 456 382 186 83 83 71 53 47 13 BsaWI w'CCGG_w Cuts at: 2202 3890 6315 6713 6987 7134 7545 2202 Size: 1688 2425 398 274 147 411 2539 Fragments arranged by size: 2539 2425 1688 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2357 2387 2633 2663 3328 Size: 30 165 30 954 30 246 30 665 Cuts at: 3328 3358 3585 3615 3980 4010 4193 4223 4505 Size: 30 227 30 365 30 183 30 282 Cuts at: 4505 4535 1178 Size: 30 4525 Fragments arranged by size: 4525 954 665 365 282 246 227 183 165 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 2339 3200 4100 4916 1186 Size: 1150 3 861 900 816 4152 Fragments arranged by size: 4152 1150 900 861 816 3 BseYI C'CCAG_C Cuts at: 2969 2994 3225 3362 4125 4215 4310 4440 4863 Size: 25 231 137 763 90 95 130 423 Cuts at: 4863 5030 5091 7085 7581 2969 Size: 167 61 1994 496 3270 Fragments arranged by size: 3270 1994 763 496 423 231 167 137 130 95 90 61 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2261 3728 4982 1163 Size: 573 525 1467 1254 4063 Fragments arranged by size: 4063 1467 1254 573 525 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2187 2871 2920 4470 4917 5332 Size: 275 186 186 684 49 1550 447 415 Cuts at: 5332 6093 7121 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1550 1028 761 684 447 415 275 186 186 49 BsiHKAI G_wGCw'C Cuts at: 2367 2412 2682 3936 3987 4385 5943 6028 7099 Size: 45 270 1254 51 398 1558 85 1071 Cuts at: 7099 2367 Size: 3150 Fragments arranged by size: 3150 1558 1254 1071 398 270 85 51 45 BsmI GAATG_Cn' Cuts at: 5393 5486 5393 Size: 93 7789 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2040 2367 2412 2682 2824 3192 Size: 399 223 27 327 45 270 142 368 Cuts at: 3192 3395 3552 3673 3683 3713 3936 3987 4073 Size: 203 157 121 10 30 223 51 86 Cuts at: 4073 4385 4821 4871 5058 5201 5943 6028 7099 Size: 312 436 50 187 143 742 85 1071 Cuts at: 7099 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 436 399 368 327 312 270 223 223 203 187 157 143 142 121 86 85 51 50 45 30 27 10 BspEI T'CCGG_A Cuts at: 3890 7545 3890 Size: 3655 4227 BspHI T'CATG_A Cuts at: 5771 7501 5771 Size: 1730 6152 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3547 3652 4363 4516 4949 5237 844 Size: 2703 105 711 153 433 288 3489 Fragments arranged by size: 3489 2703 711 433 288 153 105 BsrBI CCG'CTC Cuts at: 1394 1823 2063 2087 4671 5769 1394 Size: 429 240 24 2584 1098 3507 Fragments arranged by size: 3507 2584 1098 429 240 24 BsrDI GCAATG_nn' Cuts at: 65 1248 3501 6377 6551 65 Size: 1183 2253 2876 174 1396 Fragments arranged by size: 2876 2253 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2603 2919 3198 4465 4774 4859 4988 Size: 110 793 316 279 1267 309 85 129 Cuts at: 4988 5109 6526 6713 1700 Size: 121 1417 187 2869 Fragments arranged by size: 2869 1417 1267 793 316 309 279 187 129 121 110 85 BsrGI T'GTAC_A Cuts at: 96 2853 4164 96 Size: 2757 1311 3814 Fragments arranged by size: 3814 2757 1311 BssHII G'CGCG_C Cuts at: 1925 4700 1925 Size: 2775 5107 BssSI C'ACGA_G Cuts at: 2033 5942 6954 2033 Size: 3909 1012 2961 Fragments arranged by size: 3909 2961 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2284 2979 3077 4017 4848 794 Size: 1490 695 98 940 831 3828 Fragments arranged by size: 3828 1490 940 831 695 98 BstBI TT'CG_AA Cuts at: 6686 6686 Size: 7882 BstEII G'GTnAC_C Cuts at: 1184 3454 1184 Size: 2270 5612 BstXI CCAn_nnnn'nTGG Cuts at: 2716 3808 4486 2716 Size: 1092 678 6112 Fragments arranged by size: 6112 1092 678 BstYI r'GATC_y Cuts at: 1972 2343 3294 4194 5212 5579 5964 5981 7433 Size: 371 951 900 1018 367 385 17 1452 Cuts at: 7433 7519 7531 7877 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1018 951 900 385 371 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2648 3339 3951 4507 4520 4890 Size: 552 939 644 691 612 556 13 370 Cuts at: 4890 513 Size: 3505 Fragments arranged by size: 3505 939 691 644 612 556 552 370 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7143 Fragments arranged by size: 7143 683 56 BtsI GCAGTG_nn' Cuts at: 2270 2566 2731 3634 3681 4386 4606 4643 5398 Size: 296 165 903 47 705 220 37 755 Cuts at: 5398 6192 6212 2270 Size: 794 20 3940 Fragments arranged by size: 3940 903 794 755 705 296 220 165 47 37 20 ClaI AT'CG_AT Cuts at: 1554 3419 1554 Size: 1865 6017 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2098 2133 3585 3620 529 Size: 35 1534 35 1452 35 4791 Fragments arranged by size: 4791 1534 1452 35 35 35 DraI TTT'AAA Cuts at: 5186 5186 Size: 7882 DraIII CAC_nnn'GTG Cuts at: 2035 3077 2035 Size: 1042 6840 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3020 3785 6889 809 Size: 507 1704 765 3104 1802 Fragments arranged by size: 3104 1802 1704 765 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2106 2184 Size: 54 1425 50 275 18 258 18 78 Cuts at: 2184 2601 2868 2917 3091 3196 3798 3849 4257 Size: 417 267 49 174 105 602 51 408 Cuts at: 4257 4467 4517 4914 5107 5329 6218 7847 8 Size: 210 50 397 193 222 889 1629 43 Fragments arranged by size: 1629 1425 889 602 417 408 397 275 267 258 222 210 193 174 105 78 54 51 50 50 49 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2184 2868 2917 4467 4914 5329 1537 Size: 275 372 684 49 1550 447 415 4090 Fragments arranged by size: 4090 1550 684 447 415 372 275 49 EarI CTCTTCn'nnn_ Cuts at: 1353 5812 1353 Size: 4459 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6469 6843 6989 387 Size: 1064 138 4880 374 146 1280 Fragments arranged by size: 4880 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2410 3934 2410 Size: 1524 6358 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2282 2285 2339 2614 2912 3320 3587 Size: 393 650 3 54 275 298 408 267 Cuts at: 3587 3812 4220 4709 4781 5945 6542 7329 7597 Size: 225 408 489 72 1164 597 787 268 Cuts at: 7597 1239 Size: 1524 Fragments arranged by size: 1524 1164 787 650 597 489 408 408 393 298 275 268 267 225 72 54 3 EcoNI CCTnn'n_nnAGG Cuts at: 2321 3008 3941 2321 Size: 687 933 6262 Fragments arranged by size: 6262 933 687 EcoO109I rG'GnC_Cy Cuts at: 2429 3170 3427 3576 4069 4070 4090 4627 5036 Size: 741 257 149 493 1 20 537 409 Cuts at: 5036 2429 Size: 5275 Fragments arranged by size: 5275 741 537 493 409 257 149 20 1 EcoRI G'AATT_C Cuts at: 5191 5569 5191 Size: 378 7504 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2449 2678 2698 3031 2449 Size: 229 20 333 7300 Fragments arranged by size: 7300 333 229 20 FseI GG_CCGG'CC Cuts at: 5113 5113 Size: 7882 FspI TGC'GCA Cuts at: 4021 6388 4021 Size: 2367 5515 HaeII r_GCGC'y Cuts at: 1678 4466 4549 4757 7029 1678 Size: 2788 83 208 2272 2531 Fragments arranged by size: 2788 2531 2272 208 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2438 2470 2890 2922 3162 Size: 32 135 32 866 32 420 32 240 Cuts at: 3162 3194 4193 4225 5003 5035 5157 5189 6523 Size: 32 999 32 778 32 122 32 1334 Cuts at: 6523 6555 6597 6629 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1334 999 866 778 420 240 135 122 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4045 5226 5472 669 Size: 988 281 2107 1181 246 3079 Fragments arranged by size: 3079 2107 1181 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7882 HpaI GTT'AAC Cuts at: 5472 5472 Size: 7882 KasI G'GCGC_C Cuts at: 4462 4545 4462 Size: 83 7799 KpnI G_GTAC'C Cuts at: 5207 5207 Size: 7882 MfeI C'AATT_G Cuts at: 2394 5481 2394 Size: 3087 4795 MluI A'CGCG_T Cuts at: 7758 7758 Size: 7882 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2580 6996 7180 1305 Size: 1275 4416 184 2007 Fragments arranged by size: 4416 2007 1275 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2108 3800 3851 4259 10 Size: 54 1425 343 276 1692 51 408 3633 Fragments arranged by size: 3633 1692 1425 408 343 276 54 51 MslI CAynn'nnrTG Cuts at: 518 1166 2099 2396 2685 3653 4250 5840 6199 Size: 648 933 297 289 968 597 1590 359 Cuts at: 6199 6358 518 Size: 159 2042 Fragments arranged by size: 2042 1590 968 933 648 597 359 297 289 159 MspA1I CmG'CkG Cuts at: 1606 2463 2796 3615 4283 4502 4687 4903 5016 Size: 857 333 819 668 219 185 216 113 Cuts at: 5016 5147 5298 5975 7123 7368 1606 Size: 131 151 677 1148 245 2120 Fragments arranged by size: 2120 1148 857 819 677 668 333 245 219 216 185 151 131 113 NaeI GCC'GGC Cuts at: 2605 2921 4467 4990 5111 2605 Size: 316 1546 523 121 5376 Fragments arranged by size: 5376 1546 523 316 121 NarI GG'CG_CC Cuts at: 4463 4546 4463 Size: 83 7799 NcoI C'CATG_G Cuts at: 513 1065 2004 3339 4890 513 Size: 552 939 1335 1551 3505 Fragments arranged by size: 3505 1551 1335 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7882 NgoMIV G'CCGG_C Cuts at: 2603 2919 4465 4988 5109 2603 Size: 316 1546 523 121 5376 Fragments arranged by size: 5376 1546 523 316 121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7882 NotI GC'GGCC_GC Cuts at: 5329 5329 Size: 7882 NspI r_CATG'y Cuts at: 1132 1229 3325 4981 5240 6785 1132 Size: 97 2096 1656 259 1545 2229 Fragments arranged by size: 2229 2096 1656 1545 259 97 PasI CC'CwG_GG Cuts at: 1348 2131 4325 1348 Size: 783 2194 4905 Fragments arranged by size: 4905 2194 783 PciI A'CATG_T Cuts at: 6781 6781 Size: 7882 PflMI CCAn_nnn'nTGG Cuts at: 1108 4454 4638 1108 Size: 3346 184 4352 Fragments arranged by size: 4352 3346 184 PfoI T'CCnGG_A Cuts at: 3297 3768 3789 4029 4197 5175 3297 Size: 471 21 240 168 978 6004 Fragments arranged by size: 6004 978 471 240 168 21 PmeI GTTT'AAAC Cuts at: 5186 5186 Size: 7882 PmlI CAC'GTG Cuts at: 2173 2173 Size: 7882 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5951 5983 7464 7496 5951 Size: 32 1481 32 6337 Fragments arranged by size: 6337 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2429 3170 3576 4627 5036 2429 Size: 741 406 1051 409 5275 Fragments arranged by size: 5275 1051 741 409 406 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7882 PsiI TTA'TAA Cuts at: 5452 5452 Size: 7882 PspOMI G'GGCC_C Cuts at: 2820 3669 4069 2820 Size: 849 400 6633 Fragments arranged by size: 6633 849 400 PstI C_TGCA'G Cuts at: 830 3042 3942 4908 5051 5234 830 Size: 2212 900 966 143 183 3478 Fragments arranged by size: 3478 2212 966 900 183 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7882 PvuII CAG'CTG Cuts at: 4283 4687 4903 5016 5147 4283 Size: 404 216 113 131 7018 Fragments arranged by size: 7018 404 216 131 113 SacI G_AGCT'C Cuts at: 2412 3936 2412 Size: 1524 6358 SalI G'TCGA_C Cuts at: 1936 4043 5224 1936 Size: 2107 1181 4594 Fragments arranged by size: 4594 2107 1181 SanDI GG'GwC_CC Cuts at: 2429 3576 4627 5036 2429 Size: 1147 1051 409 5275 Fragments arranged by size: 5275 1147 1051 409 SbfI CC_TGCA'GG Cuts at: 5051 5234 5051 Size: 183 7699 ScaI AGT'ACT Cuts at: 6130 7842 6130 Size: 1712 6170 SfcI C'TryA_G Cuts at: 826 1045 3038 3938 3976 4904 5047 5230 6365 Size: 219 1993 900 38 928 143 183 1135 Cuts at: 6365 7046 7237 826 Size: 681 191 1471 Fragments arranged by size: 1993 1471 1135 928 900 681 219 191 183 143 38 SfoI GGC'GCC Cuts at: 4464 4547 4464 Size: 83 7799 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7882 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7882 SmaI CCC'GGG Cuts at: 1872 2637 3512 4299 5138 5209 1872 Size: 765 875 787 839 71 4545 Fragments arranged by size: 4545 875 839 787 765 71 SmlI C'TyrA_G Cuts at: 820 1017 1943 3005 5986 6887 7149 820 Size: 197 926 1062 2981 901 262 1553 Fragments arranged by size: 2981 1553 1062 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5673 493 Size: 5180 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7882 SphI G_CATG'C Cuts at: 1132 3325 4981 5240 1132 Size: 2193 1656 259 3774 Fragments arranged by size: 3774 2193 1656 259 SspI AAT'ATT Cuts at: 5 52 5806 5 Size: 47 5754 2081 Fragments arranged by size: 5754 2081 47 StuI AGG'CCT Cuts at: 1517 3490 1517 Size: 1973 5909 StyI C'CwwG_G Cuts at: 513 1065 2004 2049 2433 2508 3339 4651 4890 Size: 552 939 45 384 75 831 1312 239 Cuts at: 4890 5321 513 Size: 431 3074 Fragments arranged by size: 3074 1312 939 831 552 431 384 239 75 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2755 2768 2983 4324 4454 5059 5909 Size: 1255 1175 13 215 1341 130 605 850 Cuts at: 5909 5926 6079 6264 6709 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1341 1255 1175 850 605 445 215 185 153 130 17 13 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2853 4164 6128 Size: 274 80 33 51 1443 876 1311 1964 Cuts at: 6128 7840 96 Size: 1712 138 Fragments arranged by size: 1964 1712 1443 1311 876 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2060 2092 2151 2183 2357 2389 2633 2665 2946 Size: 32 59 32 174 32 244 32 281 Cuts at: 2946 2978 3328 3360 3585 3617 4505 4537 5055 Size: 32 350 32 225 32 888 32 518 Cuts at: 5055 5087 7842 7874 2060 Size: 32 2755 32 2068 Fragments arranged by size: 2755 2068 888 518 350 281 244 225 174 59 32 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3452 1316 Size: 2136 5746 XbaI T'CTAG_A Cuts at: 5218 5218 Size: 7882 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2098 3325 3493 3514 4061 4252 2098 Size: 1227 168 21 547 191 5728 Fragments arranged by size: 5728 1227 547 191 168 21 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7882 XmaI C'CCGG_G Cuts at: 1870 2635 3510 4297 5136 5207 1870 Size: 765 875 787 839 71 4545 Fragments arranged by size: 4545 875 839 787 765 71 XmnI GAAnn'nnTTC Cuts at: 6011 6011 Size: 7882 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4618 5690 276 Size: 53 83 186 936 3084 1072 2468 Fragments arranged by size: 3084 2468 1072 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI