################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII CpoI DpnII DraI DraIII Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI MaeIII MamI MboI MfeI MflI MluI MroI MseI MunI NcoI NdeI NdeII NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SalI Sau3AI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 608 GTCTAC GT^[AC][GT]AC Alw44I 697 GTGCAC G^TGCAC ApaLI 697 GTGCAC G^TGCAC BalI 548 TGGCCA TGG^CCA BbsI 84 GAAGAC GAAGAC(2/6) BcnI 123 CCGGG CC^[GC]GG BfaI 379 CTAG C^TAG BpmI 214 CTGGAG CTGGAG(16/14) BpuAI 84 GAAGAC GAAGAC(2/6) BsgI 502 CTGCAC GTGCAG(16/14) BspDI 619 ATCGAT AT^CGAT BsrBI 109 GAGCGG CCGCTC(-3/-3) BsrDI 657 CATTGC GCAATG(2/0) BsrFI 207 GCCGGC [GA]^CCGG[CT] BsrI 413 ACTGG ACTGG(1/-1) BstAPI 504 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 575 CCATGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 207 GCCGGC [GA]^CCGG[CT] ClaI 619 ATCGAT AT^CGAT DdeI 565 CTGAG C^T[ACGT]AG DrdI 529 GACCTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 564 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 345 GCGTC GACGC(5/10) HinfI 460 GACTC G^A[ACGT]TC MaeI 379 CTAG C^TAG MaeII 274 ACGT A^CGT MluNI 548 TGGCCA TGG^CCA MscI 548 TGGCCA TGG^CCA MslI 576 CATGAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 207 GCCGGC GCC^GGC NciI 123 CCGGG CC^[GC]GG NgoMIV 207 GCCGGC G^CCGGC NlaIII 576 CATG CATG^ NotI 9 GCGGCCGC GC^GGCCGC PleI 460 GACTC GAGTC(4/5) SacI 721 GAGCTC GAGCT^C SbfI 664 CCTGCAGG CCTGCA^GG Sse8387I 664 CCTGCAGG CCTGCA^GG StuI 131 AGGCCT AGG^CCT TaqI 620 TCGA T^CGA Tsp509I 384 AATT ^AATT TthHB8I 620 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 3 GGCGCC G[GA]^CG[CT]C 43 GGCGCC ApaI 18 GGGCCC GGGCC^C 488 GGGCCC AvaI 163 CCCGAG C^[CT]CG[GA]G 184 CCCGAG BbeI 3 GGCGCC GGCGC^C 43 GGCGCC BsaHI 3 GGCGCC G[GA]^CG[CT]C 43 GGCGCC BsoBI 163 CCCGAG C^[CT]CG[GA]G 184 CCCGAG EarI 70 CTCTTC CTCTTC(1/4) 682 GAAGAG EcoT14I 23 CCTTGG C^C[AT][AT]GG 356 CCAAGG HapII 123 CCGG C^CGG 208 CCGG Hin1I 3 GGCGCC G[GA]^CG[CT]C 43 GGCGCC HpaII 123 CCGG C^CGG 208 CCGG KasI 3 GGCGCC G^GCGCC 43 GGCGCC Ksp632I 70 CTCTTC CTCTTC(1/4) 682 GAAGAG MspI 123 CCGG C^CGG 208 CCGG NarI 3 GGCGCC GG^CGCC 43 GGCGCC PstI 592 CTGCAG CTGCA^G 665 CTGCAG SapI 682 GAAGAGC GCTCTTC(1/4) 69 GCTCTTC SfoI 3 GGCGCC GGC^GCC 43 GGCGCC StyI 23 CCTTGG C^C[AT][AT]GG 356 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AluI AlwNI AspHI AvaII BanI BanII BbvI BmyI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HhaI HinP1I ItaI KspI MboII MnlI MspA1I MvaI MvnI MwoI NlaIV PpuMI SacII Sau96I ScrFI SfcI TseI