(Circular) (Six-base) MAPSORT of: pFN21AE4034.seq Check: 5130 from: 1 to: 5437 pFN21AE4034 5437 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3247 278 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2614 2780 1937 Size: 677 166 4594 Fragments arranged by size: 4594 677 166 Acc65I G'GTAC_C Cuts at: 2758 2758 Size: 5437 AclI AA'CG_TT Cuts at: 1563 3564 3937 1563 Size: 2001 373 3063 Fragments arranged by size: 3063 2001 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3500 4884 5152 1632 Size: 1868 1384 268 1917 Fragments arranged by size: 1917 1868 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5240 AflIII A'CryG_T Cuts at: 1203 4336 5313 1203 Size: 3133 977 1327 Fragments arranged by size: 3133 1327 977 AgeI A'CCGG_T Cuts at: 4268 4268 Size: 5437 AhdI GACnn_n'nnGTC Cuts at: 4166 4166 Size: 5437 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5437 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2300 1184 Size: 1116 4321 AlwNI CAG_nnn'CTG Cuts at: 2407 2506 2569 3180 4752 2407 Size: 99 63 611 1572 3092 Fragments arranged by size: 3092 1572 611 99 63 ApaI G_GGCC'C Cuts at: 2027 2497 2027 Size: 470 4967 ApaLI G'TGCA_C Cuts at: 2702 3494 4650 2702 Size: 792 1156 3489 Fragments arranged by size: 3489 1156 792 ApoI r'AATT_y Cuts at: 1493 2746 2972 3124 5233 1493 Size: 1253 226 152 2109 1697 Fragments arranged by size: 2109 1697 1253 226 152 AseI AT'TA_AT Cuts at: 160 1032 3991 160 Size: 872 2959 1606 Fragments arranged by size: 2959 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 AvaI C'yCGr_G Cuts at: 1870 1943 2168 2189 2762 1870 Size: 73 225 21 573 4545 Fragments arranged by size: 4545 573 225 73 21 BamHI G'GATC_C Cuts at: 2767 3134 2767 Size: 367 5070 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2048 2696 2758 4213 Size: 325 217 627 221 40 648 62 1455 Cuts at: 4213 618 Size: 1842 Fragments arranged by size: 1842 1455 648 627 325 221 217 62 40 BanII G_rGCy'C Cuts at: 1391 2027 2069 2497 2730 2756 1391 Size: 636 42 428 233 26 4072 Fragments arranged by size: 4072 636 428 233 42 26 BbeI G_GCGC'C Cuts at: 2012 2052 2012 Size: 40 5397 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2096 928 Size: 971 197 4269 Fragments arranged by size: 4269 971 197 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3628 3662 1903 Size: 34 1691 34 3678 Fragments arranged by size: 3678 1691 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3329 4539 3329 Size: 1210 4227 BclI T'GATC_A Cuts at: 1548 1548 Size: 5437 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4048 136 Size: 107 122 71 631 605 2376 1525 Fragments arranged by size: 2376 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5432 5432 Size: 5437 BlpI GC'TnA_GC Cuts at: 2854 2854 Size: 5437 Bme1580I G_kGCm'C Cuts at: 1790 2027 2497 2706 3498 4654 1790 Size: 237 470 209 792 1156 2573 Fragments arranged by size: 2573 1156 792 470 237 209 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5437 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3168 4126 442 Size: 416 53 83 399 24 1751 958 1753 Fragments arranged by size: 1753 1751 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5437 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2749 2749 Size: 5437 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 4097 1239 Size: 1001 1857 2579 Fragments arranged by size: 2579 1857 1001 Bpu10I CC'TnA_GC Cuts at: 2570 2570 Size: 5437 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3562 4427 4725 3562 Size: 865 298 4274 Fragments arranged by size: 4274 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4100 882 Size: 617 2601 2219 Fragments arranged by size: 2601 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3228 493 Size: 711 2024 2702 Fragments arranged by size: 2702 2024 711 BsaBI GATnn'nnATC Cuts at: 5431 5431 Size: 5437 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2049 3244 3626 Size: 53 83 186 936 476 40 1195 382 Cuts at: 3626 4261 275 Size: 635 1451 Fragments arranged by size: 1451 1195 936 635 476 382 186 83 53 40 BsaWI w'CCGG_w Cuts at: 3870 4268 4542 4689 5100 3870 Size: 398 274 147 411 4207 Fragments arranged by size: 4207 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5212 Fragments arranged by size: 5212 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2213 2222 2237 2720 1186 Size: 1027 9 15 483 3903 Fragments arranged by size: 3903 1027 483 15 9 BseYI C'CCAG_C Cuts at: 2020 2041 2062 2121 2251 2608 4640 5136 2020 Size: 21 21 59 130 357 2032 496 2321 Fragments arranged by size: 2321 2032 496 357 130 59 21 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2492 1163 Size: 573 756 4108 Fragments arranged by size: 4108 756 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2018 2104 2110 2887 3648 4676 Size: 275 186 17 86 6 777 761 1028 Cuts at: 4676 1540 Size: 2301 Fragments arranged by size: 2301 1028 777 761 275 186 86 17 6 BsiHKAI G_wGCw'C Cuts at: 2076 2279 2706 2730 3498 3583 4654 2076 Size: 203 427 24 768 85 1071 2859 Fragments arranged by size: 2859 1071 768 427 203 85 24 BsmI GAATG_Cn' Cuts at: 2948 3041 2948 Size: 93 5344 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2069 2076 2279 2497 2706 2730 Size: 399 237 42 7 203 218 209 24 Cuts at: 2730 2756 3498 3583 4654 1391 Size: 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 237 218 209 203 85 42 26 24 7 BspEI T'CCGG_A Cuts at: 5100 5100 Size: 5437 BspHI T'CATG_A Cuts at: 3326 5056 3326 Size: 1730 3707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2388 2415 2544 2792 844 Size: 1544 27 129 248 3489 Fragments arranged by size: 3489 1544 248 129 27 BsrBI CCG'CTC Cuts at: 1394 1823 2116 3324 1394 Size: 429 293 1208 3507 Fragments arranged by size: 3507 1208 429 293 BsrDI GCAATG_nn' Cuts at: 65 1248 2661 3932 4106 65 Size: 1183 1413 1271 174 1396 Fragments arranged by size: 1413 1396 1271 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2212 4081 4268 1700 Size: 110 402 1869 187 2869 Fragments arranged by size: 2869 1869 402 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5437 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5437 BssSI C'ACGA_G Cuts at: 3497 4509 3497 Size: 1012 4425 BstAPI GCAn_nnn'nTGC Cuts at: 794 2515 794 Size: 1721 3716 BstBI TT'CG_AA Cuts at: 4241 4241 Size: 5437 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5437 BstXI CCAn_nnnn'nTGG Cuts at: 2587 2587 Size: 5437 BstYI r'GATC_y Cuts at: 1972 2767 3134 3519 3536 4988 5074 5086 5432 Size: 795 367 385 17 1452 86 12 346 Cuts at: 5432 1972 Size: 1977 Fragments arranged by size: 1977 1452 795 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2012 2052 2104 513 Size: 552 939 8 40 52 3846 Fragments arranged by size: 3846 939 552 52 40 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4698 Fragments arranged by size: 4698 683 56 BtsI GCAGTG_nn' Cuts at: 2953 3747 3767 2953 Size: 794 20 4623 Fragments arranged by size: 4623 794 20 ClaI AT'CG_AT Cuts at: 1554 2625 1554 Size: 1071 4366 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5402 DraI TTT'AAA Cuts at: 2741 2741 Size: 5437 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2540 4444 809 Size: 507 1224 1904 1802 Fragments arranged by size: 1904 1802 1224 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2015 2101 2107 Size: 54 1425 50 275 18 185 86 6 Cuts at: 2107 2553 2884 3773 5402 8 Size: 446 331 889 1629 43 Fragments arranged by size: 1629 1425 889 446 331 275 185 86 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2015 2101 2107 2884 1537 Size: 275 203 86 6 777 4090 Fragments arranged by size: 4090 777 275 203 86 6 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 2682 3367 1353 Size: 728 601 685 3423 Fragments arranged by size: 3423 728 685 601 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4024 4398 4544 387 Size: 1064 138 2435 374 146 1280 Fragments arranged by size: 2435 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2728 2728 Size: 5437 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 3500 4097 4884 5152 1239 Size: 393 608 1260 597 787 268 1524 Fragments arranged by size: 1524 1260 787 608 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2573 2573 Size: 5437 EcoO109I rG'GnC_Cy Cuts at: 2024 2185 2527 2566 2675 2024 Size: 161 342 39 109 4786 Fragments arranged by size: 4786 342 161 109 39 EcoRI G'AATT_C Cuts at: 2746 3124 2746 Size: 378 5059 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2113 2113 Size: 5437 FspI TGC'GCA Cuts at: 3943 3943 Size: 5437 HaeII r_GCGC'y Cuts at: 1678 2012 2042 2052 2122 2219 4584 1678 Size: 334 30 10 70 97 2365 2531 Fragments arranged by size: 2531 2365 334 97 70 30 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4078 4110 4152 4184 1373 Size: 32 135 32 2506 32 42 32 2626 Fragments arranged by size: 2626 2506 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2781 3027 669 Size: 988 281 843 246 3079 Fragments arranged by size: 3079 988 843 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5437 HpaI GTT'AAC Cuts at: 3027 3027 Size: 5437 KasI G'GCGC_C Cuts at: 2008 2048 2008 Size: 40 5397 KpnI G_GTAC'C Cuts at: 2762 2762 Size: 5437 MfeI C'AATT_G Cuts at: 3036 3036 Size: 5437 MluI A'CGCG_T Cuts at: 5313 5313 Size: 5437 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4551 4735 1305 Size: 3246 184 2007 Fragments arranged by size: 3246 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2555 10 Size: 54 1425 343 723 2892 Fragments arranged by size: 2892 1425 723 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2585 3395 3754 3913 518 Size: 648 1419 810 359 159 2042 Fragments arranged by size: 2042 1419 810 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2020 2054 2106 2311 2602 2635 2853 Size: 408 6 34 52 205 291 33 218 Cuts at: 2853 3530 4678 4923 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 408 291 245 218 205 52 34 33 6 NaeI GCC'GGC Cuts at: 2214 2214 Size: 5437 NarI GG'CG_CC Cuts at: 2009 2049 2009 Size: 40 5397 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3946 Fragments arranged by size: 3946 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5437 NgoMIV G'CCGG_C Cuts at: 2212 2212 Size: 5437 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5437 NotI GC'GGCC_GC Cuts at: 2015 2884 2015 Size: 869 4568 NspI r_CATG'y Cuts at: 1132 1229 2795 4340 1132 Size: 97 1566 1545 2229 Fragments arranged by size: 2229 1566 1545 97 PasI CC'CwG_GG Cuts at: 1348 2490 1348 Size: 1142 4295 PciI A'CATG_T Cuts at: 4336 4336 Size: 5437 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5437 PmeI GTTT'AAAC Cuts at: 2741 2741 Size: 5437 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3506 3538 5019 5051 3506 Size: 32 1481 32 3892 Fragments arranged by size: 3892 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2185 2527 2566 2675 2185 Size: 342 39 109 4947 Fragments arranged by size: 4947 342 109 39 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5437 PsiI TTA'TAA Cuts at: 3007 3007 Size: 5437 PspOMI G'GGCC_C Cuts at: 2023 2493 2023 Size: 470 4967 PstI C_TGCA'G Cuts at: 830 2601 2674 2789 830 Size: 1771 73 115 3478 Fragments arranged by size: 3478 1771 115 73 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5437 SacI G_AGCT'C Cuts at: 2730 2730 Size: 5437 SacII CC_GC'GG Cuts at: 2015 2055 2107 2015 Size: 40 52 5345 Fragments arranged by size: 5345 52 40 SalI G'TCGA_C Cuts at: 1936 2779 1936 Size: 843 4594 SanDI GG'GwC_CC Cuts at: 2185 2185 Size: 5437 SapI GCTCTTCn'nnn_ Cuts at: 2081 2682 2081 Size: 601 4836 SbfI CC_TGCA'GG Cuts at: 2674 2789 2674 Size: 115 5322 ScaI AGT'ACT Cuts at: 3685 5397 3685 Size: 1712 3725 SfcI C'TryA_G Cuts at: 826 1045 2597 2615 2670 2785 3920 4601 4792 Size: 219 1552 18 55 115 1135 681 191 Cuts at: 4792 826 Size: 1471 Fragments arranged by size: 1552 1471 1135 681 219 191 115 55 18 SfoI GGC'GCC Cuts at: 2010 2050 2010 Size: 40 5397 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5437 SmaI CCC'GGG Cuts at: 1872 2764 1872 Size: 892 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3541 4442 4704 820 Size: 197 926 1598 901 262 1553 Fragments arranged by size: 1598 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3228 493 Size: 2735 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5437 SphI G_CATG'C Cuts at: 1132 2795 1132 Size: 1663 3774 SspI AAT'ATT Cuts at: 5 52 3361 5 Size: 47 3309 2081 Fragments arranged by size: 3309 2081 47 StuI AGG'CCT Cuts at: 1517 2138 1517 Size: 621 4816 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 2361 2876 513 Size: 552 939 24 333 515 3074 Fragments arranged by size: 3074 939 552 515 333 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2490 3464 3481 3634 3819 4264 325 Size: 1255 910 974 17 153 185 445 1498 Fragments arranged by size: 1498 1255 974 910 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3683 5395 96 Size: 274 80 33 51 1443 1706 1712 138 Fragments arranged by size: 1712 1706 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5397 5429 5397 Size: 32 5405 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5437 XbaI T'CTAG_A Cuts at: 2773 2773 Size: 5437 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5437 XmaI C'CCGG_G Cuts at: 1870 2762 1870 Size: 892 4545 XmnI GAAnn'nnTTC Cuts at: 3566 3566 Size: 5437 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3245 276 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SexAI SfiI SrfI SwaI XcmI