(Linear) (Six-base) MAPSORT of: KIEE4034.seq Check: 3457 from: 1 to: 732 KIEE4034.orf With 182 enzymes: SgfI * July 3, 2012 18:25 .. AccI GT'mk_AC Cuts at: 0 609 732 Size: 609 123 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 295 732 Size: 295 437 AlwNI CAG_nnn'CTG Cuts at: 0 402 501 564 732 Size: 402 99 63 168 Fragments arranged by size: 402 168 99 63 ApaI G_GGCC'C Cuts at: 0 22 492 732 Size: 22 470 240 Fragments arranged by size: 470 240 22 ApaLI G'TGCA_C Cuts at: 0 697 732 Size: 697 35 AvaI C'yCGr_G Cuts at: 0 163 184 732 Size: 163 21 548 Fragments arranged by size: 548 163 21 BanI G'GyrC_C Cuts at: 0 3 43 691 732 Size: 3 40 648 41 Fragments arranged by size: 648 41 40 3 BanII G_rGCy'C Cuts at: 0 22 64 492 725 732 Size: 22 42 428 233 7 Fragments arranged by size: 428 233 42 22 7 BbeI G_GCGC'C Cuts at: 0 7 47 732 Size: 7 40 685 Fragments arranged by size: 685 40 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 91 732 Size: 91 641 Bme1580I G_kGCm'C Cuts at: 0 22 492 701 732 Size: 22 470 209 31 Fragments arranged by size: 470 209 31 22 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 732 Size: 235 497 Bpu10I CC'TnA_GC Cuts at: 0 565 732 Size: 565 167 BsaHI Gr'CG_yC Cuts at: 0 4 44 732 Size: 4 40 688 Fragments arranged by size: 688 40 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 208 217 232 715 732 Size: 208 9 15 483 17 Fragments arranged by size: 483 208 17 15 9 BseYI C'CCAG_C Cuts at: 0 15 36 57 116 246 603 732 Size: 15 21 21 59 130 357 129 Fragments arranged by size: 357 130 129 59 21 21 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 487 732 Size: 487 245 BsiEI CG_ry'CG Cuts at: 0 13 99 105 732 Size: 13 86 6 627 Fragments arranged by size: 627 86 13 6 BsiHKAI G_wGCw'C Cuts at: 0 71 274 701 725 732 Size: 71 203 427 24 7 Fragments arranged by size: 427 203 71 24 7 Bsp1286I G_dGCh'C Cuts at: 0 22 64 71 274 492 701 725 732 Size: 22 42 7 203 218 209 24 7 Fragments arranged by size: 218 209 203 42 24 22 7 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 383 410 539 732 Size: 383 27 129 193 Fragments arranged by size: 383 193 129 27 BsrBI CCG'CTC Cuts at: 0 111 732 Size: 111 621 BsrDI GCAATG_nn' Cuts at: 0 656 732 Size: 656 76 BsrFI r'CCGG_y Cuts at: 0 207 732 Size: 207 525 BstAPI GCAn_nnn'nTGC Cuts at: 0 510 732 Size: 510 222 BstXI CCAn_nnnn'nTGG Cuts at: 0 582 732 Size: 582 150 BtgI C'CryG_G Cuts at: 0 7 47 99 732 Size: 7 40 52 633 Fragments arranged by size: 633 52 40 7 ClaI AT'CG_AT Cuts at: 0 620 732 Size: 620 112 DrdI GACnn_nn'nnGTC Cuts at: 0 535 732 Size: 535 197 EaeI y'GGCC_r Cuts at: 0 10 96 102 548 732 Size: 10 86 6 446 184 Fragments arranged by size: 446 184 86 10 6 EagI C'GGCC_G Cuts at: 0 10 96 102 732 Size: 10 86 6 630 Fragments arranged by size: 630 86 10 6 EarI CTCTTCn'nnn_ Cuts at: 0 76 677 732 Size: 76 601 55 Fragments arranged by size: 601 76 55 EcoICRI GAG'CTC Cuts at: 0 723 732 Size: 723 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 732 Size: 235 497 EcoNI CCTnn'n_nnAGG Cuts at: 0 568 732 Size: 568 164 EcoO109I rG'GnC_Cy Cuts at: 0 19 180 522 561 670 732 Size: 19 161 342 39 109 62 Fragments arranged by size: 342 161 109 62 39 19 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 108 732 Size: 108 624 HaeII r_GCGC'y Cuts at: 0 7 37 47 117 214 732 Size: 7 30 10 70 97 518 Fragments arranged by size: 518 97 70 30 10 7 KasI G'GCGC_C Cuts at: 0 3 43 732 Size: 3 40 689 Fragments arranged by size: 689 40 3 MscI TGG'CCA Cuts at: 0 550 732 Size: 550 182 MslI CAynn'nnrTG Cuts at: 0 580 732 Size: 580 152 MspA1I CmG'CkG Cuts at: 0 9 15 49 101 306 597 630 732 Size: 9 6 34 52 205 291 33 102 Fragments arranged by size: 291 205 102 52 34 33 9 6 NaeI GCC'GGC Cuts at: 0 209 732 Size: 209 523 NarI GG'CG_CC Cuts at: 0 4 44 732 Size: 4 40 688 Fragments arranged by size: 688 40 4 NgoMIV G'CCGG_C Cuts at: 0 207 732 Size: 207 525 NotI GC'GGCC_GC Cuts at: 0 10 732 Size: 10 722 PasI CC'CwG_GG Cuts at: 0 485 732 Size: 485 247 PpuMI rG'GwC_Cy Cuts at: 0 180 522 561 670 732 Size: 180 342 39 109 62 Fragments arranged by size: 342 180 109 62 39 PspOMI G'GGCC_C Cuts at: 0 18 488 732 Size: 18 470 244 Fragments arranged by size: 470 244 18 PstI C_TGCA'G Cuts at: 0 596 669 732 Size: 596 73 63 Fragments arranged by size: 596 73 63 SacI G_AGCT'C Cuts at: 0 725 732 Size: 725 7 SacII CC_GC'GG Cuts at: 0 10 50 102 732 Size: 10 40 52 630 Fragments arranged by size: 630 52 40 10 SanDI GG'GwC_CC Cuts at: 0 180 732 Size: 180 552 SapI GCTCTTCn'nnn_ Cuts at: 0 76 677 732 Size: 76 601 55 Fragments arranged by size: 601 76 55 SbfI CC_TGCA'GG Cuts at: 0 669 732 Size: 669 63 SfcI C'TryA_G Cuts at: 0 592 610 665 732 Size: 592 18 55 67 Fragments arranged by size: 592 67 55 18 SfoI GGC'GCC Cuts at: 0 5 45 732 Size: 5 40 687 Fragments arranged by size: 687 40 5 StuI AGG'CCT Cuts at: 0 133 732 Size: 133 599 StyI C'CwwG_G Cuts at: 0 23 356 732 Size: 23 333 376 Fragments arranged by size: 376 333 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 485 732 Size: 485 247 Enzymes that do cut: AccI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI Bme1580I BpmI Bpu10I BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI BtgI ClaI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII KasI MscI MslI MspA1I NaeI NarI NgoMIV NotI PasI PpuMI PspOMI PstI SacI SacII SanDI SapI SbfI SfcI SfoI StuI StyI TaqII Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI CspCI DraI DraIII EciI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI