Restriction map for
pFN21AB7302
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# NO CUT ENZYMES ( 85 enzymes) #
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Acc65I AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BsiWI BsmI BspDI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI Eam1105I Eco47III EcoO65I EcoRV
EcoT22I FbaI FspI HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 2944 GTCTAC GT^[AC][GT]AC
AccIII 3227 TCCGGA T^CCGGA
AflIII 2360 ACGTGT A^C[GA][CT]GT
Alw44I 414 GTGCAC G^TGCAC
ApaLI 414 GTGCAC G^TGCAC
BalI 1644 TGGCCA TGG^CCA
BsaAI 2359 TACGTG [CT]AC^GT[GA]
BsaBI 2994 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3227 TCCGGA [AT]^CCGG[AT]
BseAI 3227 TCCGGA T^CCGGA
BsmBI 1478 CGTCTC CGTCTC(1/5)
BspEI 3227 TCCGGA T^CCGGA
BspHI 317 TCATGA T^CATGA
BsrBI 1729 GAGCGG CCGCTC(-3/-3)
DraI 2298 TTTAAA TTT^AAA
DrdI 1762 GACCCTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1664 CCACGG C^C[GA][CT]GG
EcoNI 915 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2412 GAATTC G^AATTC
FseI 3641 GGCCGGCC GGCCGG^CC
HindIII 3093 AAGCTT A^AGCTT
MamI 2994 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1644 TGGCCA TGG^CCA
MroI 3227 TCCGGA T^CCGGA
MscI 1644 TGGCCA TGG^CCA
NaeI 3642 GCCGGC GCC^GGC
NgoMIV 3642 GCCGGC G^CCGGC
RcaI 317 TCATGA T^CATGA
SexAI 2755 ACCTGGT A^CC[AT]GGT
StuI 457 AGGCCT AGG^CCT
XcmI 1600 CCAGTGGTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AatII 1768 GACGTC GACGT^C
3478 GACGTC
AcsI 2412 GAATTC [GA]^AATT[CT]
2712 GAATTT
ApoI 2412 GAATTC [GA]^AATT[CT]
2712 GAATTT
Asp700I 1716 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2589 GAATTATTTC
AspI 141 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
907 GACCTTGTC
BanI 687 GGCACC G^G[CT][GA]CC
3310 GGCACC
BfaI 455 CTAG C^TAG
916 CTAG
BglI 436 GCCCTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1660 GCCTCCACGGC
BlpI 1514 GCTCAGC GC^T[ACGT]AGC
2226 GCTCAGC
Bpu1102I 1514 GCTCAGC GC^T[ACGT]AGC
2226 GCTCAGC
Bsp1407I 2363 TGTACA T^GTACA
2996 TGTACA
BsrGI 2363 TGTACA T^GTACA
2996 TGTACA
BstAPI 804 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2781 GCAAGGCATGC
BstXI 758 CCAGGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3131 CCAACGTGGTGG
CelII 1514 GCTCAGC GC^T[ACGT]AGC
2226 GCTCAGC
DraIII 408 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3661 CACTCGGTG
EagI 2985 CGGCCG C^GGCCG
3644 CGGCCG
EclXI 2985 CGGCCG C^GGCCG
3644 CGGCCG
Eco52I 2985 CGGCCG C^GGCCG
3644 CGGCCG
MaeI 455 CTAG C^TAG
916 CTAG
MseI 2299 TTAA T^TAA
2324 TTAA
PflFI 141 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
907 GACCTTGTC
PvuII 2581 CAGCTG CAG^CTG
2682 CAGCTG
SfiI 435 GGCCCTTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1659 GGCCTCCACGGCC
SmlI 439 CTTGAG C^T[CT][GA]AG
1573 CTCAAG
SphI 2786 GCATGC GCATG^C
3245 GCATGC
SspBI 2363 TGTACA T^GTACA
2996 TGTACA
TaqI 2318 TCGA T^CGA
2849 TCGA
Tru9I 2299 TTAA T^TAA
2324 TTAA
Tth111I 141 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
907 GACCTTGTC
TthHB8I 2318 TCGA T^CGA
2849 TCGA
XmnI 1716 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2589 GAATTATTTC
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanII BbsI BbvCI
BbvI BcnI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TfiI TseI Tsp45I Tsp509I
TspRI Van91I XhoII XmaCI XmaI