(Circular) (Six-base) MAPSORT of: pFN21AB7302.seq Check: 1545 from: 1 to: 8458 pFN21AB7302 8458 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3145 3397 3145 Size: 252 8206 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3777 5487 6268 278 Size: 53 83 186 936 2241 1710 781 2468 Fragments arranged by size: 2468 2241 1710 936 781 186 83 53 AccI GT'mk_AC Cuts at: 1937 4950 5801 1937 Size: 3013 851 4594 Fragments arranged by size: 4594 3013 851 Acc65I G'GTAC_C Cuts at: 5779 5779 Size: 8458 AclI AA'CG_TT Cuts at: 1563 6585 6958 1563 Size: 5022 373 3063 Fragments arranged by size: 5022 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3189 3276 3312 3591 3873 4223 4949 6521 Size: 1557 87 36 279 282 350 726 1572 Cuts at: 6521 7905 8173 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1572 1557 1384 726 350 282 279 268 87 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8261 AflIII A'CryG_T Cuts at: 1203 4365 7357 8334 1203 Size: 3162 2992 977 1327 Fragments arranged by size: 3162 2992 1327 977 AgeI A'CCGG_T Cuts at: 7289 7289 Size: 8458 AhdI GACnn_n'nnGTC Cuts at: 7187 7187 Size: 8458 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8458 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8458 AlwNI CAG_nnn'CTG Cuts at: 2389 2500 2573 3217 3715 4591 5716 6201 7773 Size: 111 73 644 498 876 1125 485 1572 Cuts at: 7773 2389 Size: 3074 Fragments arranged by size: 3074 1572 1125 876 644 498 485 111 73 ApaI G_GGCC'C Cuts at: 2266 2619 5407 5696 2266 Size: 353 2788 289 5028 Fragments arranged by size: 5028 2788 353 289 ApaLI G'TGCA_C Cuts at: 2419 6515 7671 2419 Size: 4096 1156 3206 Fragments arranged by size: 4096 3206 1156 ApoI r'AATT_y Cuts at: 1493 4417 4717 5767 5993 6145 8254 1493 Size: 2924 300 1050 226 152 2109 1697 Fragments arranged by size: 2924 2109 1697 1050 300 226 152 AseI AT'TA_AT Cuts at: 160 1032 7012 160 Size: 872 5980 1606 Fragments arranged by size: 5980 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8458 AvaI C'yCGr_G Cuts at: 1870 1943 2631 3526 5307 5573 5581 5689 5783 Size: 73 688 895 1781 266 8 108 94 Cuts at: 5783 1870 Size: 4545 Fragments arranged by size: 4545 1781 895 688 266 108 94 73 8 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2973 3006 2973 Size: 33 8425 BamHI G'GATC_C Cuts at: 5788 6155 5788 Size: 367 8091 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2692 5315 5779 7234 618 Size: 325 217 627 905 2623 464 1455 1842 Fragments arranged by size: 2623 1842 1455 905 627 464 325 217 BanII G_rGCy'C Cuts at: 1391 2266 2619 2709 3315 3579 3797 5407 5477 Size: 875 353 90 606 264 218 1610 70 Cuts at: 5477 5580 5600 5658 5690 5696 5777 1391 Size: 103 20 58 32 6 81 4072 Fragments arranged by size: 4072 1610 875 606 353 264 218 103 90 81 70 58 32 20 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2063 3186 4532 4573 928 Size: 971 164 1123 1346 41 4813 Fragments arranged by size: 4813 1346 1123 971 164 41 BbvCI CC'TCA_GC Cuts at: 2034 2517 3351 4352 2034 Size: 483 834 1001 6140 Fragments arranged by size: 6140 1001 834 483 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6649 6683 1903 Size: 34 4712 34 3678 Fragments arranged by size: 4712 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6350 7560 6350 Size: 1210 7248 BclI T'GATC_A Cuts at: 1548 1548 Size: 8458 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2447 3671 7069 Size: 107 122 71 631 605 775 1224 3398 Cuts at: 7069 136 Size: 1525 Fragments arranged by size: 3398 1525 1224 775 631 605 122 107 71 BglII A'GATC_T Cuts at: 8453 8453 Size: 8458 BlpI GC'TnA_GC Cuts at: 3520 4232 5875 3520 Size: 712 1643 6103 Fragments arranged by size: 6103 1643 712 Bme1580I G_kGCm'C Cuts at: 1790 2266 2423 2619 5318 5407 5696 6519 7675 Size: 476 157 196 2699 89 289 823 1156 Cuts at: 7675 1790 Size: 2573 Fragments arranged by size: 2699 2573 1156 823 476 289 196 157 89 BmgBI CAC'GTC Cuts at: 1318 4858 1318 Size: 3540 4918 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2415 3331 3599 Size: 416 53 83 399 24 998 916 268 Cuts at: 3599 4444 4480 6189 7147 442 Size: 845 36 1709 958 1753 Fragments arranged by size: 1753 1709 998 958 916 845 416 399 268 83 53 36 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8458 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2659 2866 5518 2659 Size: 207 2652 5599 Fragments arranged by size: 5599 2652 207 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2510 2563 2577 2685 2813 2913 3692 3819 Size: 1271 53 14 108 128 100 779 127 Cuts at: 3819 5450 7118 1239 Size: 1631 1668 2579 Fragments arranged by size: 2579 1668 1631 1271 779 128 127 108 100 53 14 Bpu10I CC'TnA_GC Cuts at: 2034 2517 3351 4352 2034 Size: 483 834 1001 6140 Fragments arranged by size: 6140 1001 834 483 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2465 3563 6583 7448 7746 2465 Size: 1098 3020 865 298 3177 Fragments arranged by size: 3177 3020 1098 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2216 2630 3323 5592 7121 882 Size: 617 717 414 693 2269 1529 2219 Fragments arranged by size: 2269 2219 1529 717 693 617 414 BsaAI yAC'GTr Cuts at: 493 1204 4366 6249 493 Size: 711 3162 1883 2702 Fragments arranged by size: 3162 2702 1883 711 BsaBI GATnn'nnATC Cuts at: 5003 8452 5003 Size: 3449 5009 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2599 3774 4608 5484 Size: 53 83 186 936 1066 1175 834 876 Cuts at: 5484 5567 6265 6647 7282 275 Size: 83 698 382 635 1451 Fragments arranged by size: 1451 1175 1066 936 876 834 698 635 382 186 83 83 53 BsaWI w'CCGG_w Cuts at: 5232 6891 7289 7563 7710 8121 5232 Size: 1659 398 274 147 411 5569 Fragments arranged by size: 5569 1659 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2886 2916 1178 Size: 30 165 30 1483 30 6720 Fragments arranged by size: 6720 1483 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2150 2201 2725 2939 3074 3089 3092 3107 Size: 964 51 524 214 135 15 3 15 Cuts at: 3107 3110 3113 3116 3125 3128 3251 3287 3296 Size: 3 3 3 9 3 123 36 9 Cuts at: 3296 3311 3353 3803 3887 4274 5294 5651 5654 Size: 15 42 450 84 387 1020 357 3 Cuts at: 5654 5759 1186 Size: 105 3885 Fragments arranged by size: 3885 1020 964 524 450 387 357 214 135 123 105 84 51 42 36 15 15 15 9 9 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2013 2297 2584 2709 3808 4035 7661 8157 2013 Size: 284 287 125 1099 227 3626 496 2314 Fragments arranged by size: 3626 2314 1099 496 287 284 227 125 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2768 2891 3425 4763 4838 5045 1163 Size: 573 1032 123 534 1338 75 207 4576 Fragments arranged by size: 4576 1338 1032 573 534 207 123 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3703 4993 5652 5908 6669 7697 Size: 275 186 1702 1290 659 256 761 1028 Cuts at: 7697 1540 Size: 2301 Fragments arranged by size: 2301 1702 1290 1028 761 659 275 256 186 BsiHKAI G_wGCw'C Cuts at: 2423 3203 3579 3879 5477 5600 5667 6519 6604 Size: 780 376 300 1598 123 67 852 85 Cuts at: 6604 7675 2423 Size: 1071 3206 Fragments arranged by size: 3206 1598 1071 852 780 376 300 123 85 67 BsmI GAATG_Cn' Cuts at: 5969 6062 5969 Size: 93 8365 BsmBI CGTCTCn'nnnn_ Cuts at: 3489 3489 Size: 8458 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2266 2423 2619 2709 3203 3315 3579 Size: 399 476 157 196 90 494 112 264 Cuts at: 3579 3797 3879 5318 5407 5477 5580 5600 5658 Size: 218 82 1439 89 70 103 20 58 Cuts at: 5658 5667 5690 5696 5777 6519 6604 7675 1391 Size: 9 23 6 81 742 85 1071 2174 Fragments arranged by size: 2174 1439 1071 742 494 476 399 264 218 196 157 112 103 90 89 85 82 81 70 58 23 20 9 6 BspEI T'CCGG_A Cuts at: 5232 8121 5232 Size: 2889 5569 BspHI T'CATG_A Cuts at: 2322 6347 8077 2322 Size: 4025 1730 2703 Fragments arranged by size: 4025 2703 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3145 3397 4236 4962 5017 5813 844 Size: 2301 252 839 726 55 796 3489 Fragments arranged by size: 3489 2301 839 796 726 252 55 BsrBI CCG'CTC Cuts at: 1394 1823 3736 6345 1394 Size: 429 1913 2609 3507 Fragments arranged by size: 3507 2609 1913 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6953 7127 65 Size: 1183 5705 174 1396 Fragments arranged by size: 5705 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3702 4905 5124 5647 7102 7289 1700 Size: 110 1892 1203 219 523 1455 187 2869 Fragments arranged by size: 2869 1892 1455 1203 523 219 187 110 BsrGI T'GTAC_A Cuts at: 96 4368 5001 96 Size: 4272 633 3553 Fragments arranged by size: 4272 3553 633 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8458 BssSI C'ACGA_G Cuts at: 6518 7530 6518 Size: 1012 7446 BstAPI GCAn_nnn'nTGC Cuts at: 794 2815 4792 794 Size: 2021 1977 4460 Fragments arranged by size: 4460 2021 1977 BstBI TT'CG_AA Cuts at: 7262 7262 Size: 8458 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8458 BstXI CCAn_nnnn'nTGG Cuts at: 2770 5143 2770 Size: 2373 6085 BstYI r'GATC_y Cuts at: 1972 2385 4728 5078 5676 5712 5788 6155 6540 Size: 413 2343 350 598 36 76 367 385 Cuts at: 6540 6557 8009 8095 8107 8453 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2343 1977 1452 598 413 385 367 350 346 86 76 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2133 2217 3345 2133 Size: 84 1128 7246 Fragments arranged by size: 7246 1128 84 BtgI C'CryG_G Cuts at: 513 1065 3669 513 Size: 552 2604 5302 Fragments arranged by size: 5302 2604 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7719 Fragments arranged by size: 7719 683 56 BtsI GCAGTG_nn' Cuts at: 5974 6768 6788 5974 Size: 794 20 7644 Fragments arranged by size: 7644 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8458 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8423 DraI TTT'AAA Cuts at: 4305 5762 4305 Size: 1457 7001 DraIII CAC_nnn'GTG Cuts at: 2418 5671 2418 Size: 3253 5205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3773 7465 809 Size: 507 2457 3692 1802 Fragments arranged by size: 3692 2457 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3649 3672 4990 Size: 54 1425 50 275 18 1819 23 1318 Cuts at: 4990 5133 5421 5649 5905 6794 8423 8 Size: 143 288 228 256 889 1629 43 Fragments arranged by size: 1819 1629 1425 1318 889 288 275 256 228 143 54 50 43 23 18 EagI C'GGCC_G Cuts at: 1537 1812 4990 5649 5905 1537 Size: 275 3178 659 256 4090 Fragments arranged by size: 4090 3178 659 275 256 EarI CTCTTCn'nnn_ Cuts at: 1353 2070 2166 3003 3051 3078 3084 3102 3252 Size: 717 96 837 48 27 6 18 150 Cuts at: 3252 3288 3444 3567 3731 3864 4925 5171 5279 Size: 36 156 123 164 133 1061 246 108 Cuts at: 5279 5333 5655 6388 1353 Size: 54 322 733 3423 Fragments arranged by size: 3423 1061 837 733 717 322 246 164 156 150 133 123 108 96 54 48 36 27 18 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2123 3341 4277 5494 5768 7045 Size: 1064 138 534 1218 936 1217 274 1277 Cuts at: 7045 7419 7565 387 Size: 374 146 1280 Fragments arranged by size: 1280 1277 1218 1217 1064 936 534 374 274 146 138 EcoICRI GAG'CTC Cuts at: 3577 5475 5598 3577 Size: 1898 123 6437 Fragments arranged by size: 6437 1898 123 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2510 2563 2577 2685 2813 2913 3189 Size: 393 878 53 14 108 128 100 276 Cuts at: 3189 3276 3312 3591 3692 3819 3873 4223 4949 Size: 87 36 279 101 127 54 350 726 Cuts at: 4949 5450 6521 7118 7905 8173 1239 Size: 501 1071 597 787 268 1524 Fragments arranged by size: 1524 1071 878 787 726 597 501 393 350 279 276 268 128 127 108 101 100 87 54 53 36 14 EcoNI CCTnn'n_nnAGG Cuts at: 2924 2924 Size: 8458 EcoO109I rG'GnC_Cy Cuts at: 2046 2374 2440 2615 2755 2779 3186 4453 5185 Size: 328 66 175 140 24 407 1267 732 Cuts at: 5185 2046 Size: 5319 Fragments arranged by size: 5319 1267 732 407 328 175 140 66 24 EcoRI G'AATT_C Cuts at: 4417 5767 6145 4417 Size: 1350 378 6730 Fragments arranged by size: 6730 1350 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4585 4585 Size: 8458 FseI GG_CCGG'CC Cuts at: 5651 5651 Size: 8458 FspI TGC'GCA Cuts at: 6964 6964 Size: 8458 HaeII r_GCGC'y Cuts at: 1678 2274 2591 3690 7605 1678 Size: 596 317 1099 3915 2531 Fragments arranged by size: 3915 2531 1099 596 317 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2141 2167 2173 3764 Size: 32 135 32 563 6 26 6 1591 Cuts at: 3764 3796 5036 5068 5697 5729 7099 7131 7173 Size: 32 1240 32 629 32 1370 32 42 Cuts at: 7173 7205 1373 Size: 32 2626 Fragments arranged by size: 2626 1591 1370 1240 629 563 135 42 32 32 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 5802 6048 669 Size: 988 281 3864 246 3079 Fragments arranged by size: 3864 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 5098 748 Size: 4350 4108 HpaI GTT'AAC Cuts at: 6048 6048 Size: 8458 KpnI G_GTAC'C Cuts at: 5783 5783 Size: 8458 MfeI C'AATT_G Cuts at: 6057 6057 Size: 8458 MluI A'CGCG_T Cuts at: 8334 8334 Size: 8458 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7572 7756 1305 Size: 6267 184 2007 Fragments arranged by size: 6267 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3651 10 Size: 54 1425 343 1819 4817 Fragments arranged by size: 4817 1819 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2958 4124 4856 6416 6775 6934 518 Size: 648 1792 1166 732 1560 359 159 2042 Fragments arranged by size: 2042 1792 1560 1166 732 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3851 3955 4299 4588 4689 5874 6551 7699 Size: 2245 104 344 289 101 1185 677 1148 Cuts at: 7699 7944 1606 Size: 245 2120 Fragments arranged by size: 2245 2120 1185 1148 677 344 289 245 104 101 NaeI GCC'GGC Cuts at: 5649 5649 Size: 8458 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7906 NdeI CA'TA_TG Cuts at: 387 387 Size: 8458 NgoMIV G'CCGG_C Cuts at: 5647 5647 Size: 8458 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8458 NotI GC'GGCC_GC Cuts at: 5905 5905 Size: 8458 NspI r_CATG'y Cuts at: 1132 1229 3892 4189 4795 4927 5254 5816 7361 Size: 97 2663 297 606 132 327 562 1545 Cuts at: 7361 1132 Size: 2229 Fragments arranged by size: 2663 2229 1545 606 562 327 297 132 97 PasI CC'CwG_GG Cuts at: 1348 2042 2266 2855 2940 1348 Size: 694 224 589 85 6866 Fragments arranged by size: 6866 694 589 224 85 PciI A'CATG_T Cuts at: 7357 7357 Size: 8458 PflMI CCAn_nnn'nTGG Cuts at: 1108 2611 3715 4470 1108 Size: 1503 1104 755 5096 Fragments arranged by size: 5096 1503 1104 755 PfoI T'CCnGG_A Cuts at: 2593 2890 3047 3708 4113 5172 2593 Size: 297 157 661 405 1059 5879 Fragments arranged by size: 5879 1059 661 405 297 157 PmeI GTTT'AAAC Cuts at: 5762 5762 Size: 8458 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6527 6559 8040 8072 6527 Size: 32 1481 32 6913 Fragments arranged by size: 6913 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2046 2374 2755 2779 3186 5185 2046 Size: 328 381 24 407 1999 5319 Fragments arranged by size: 5319 1999 407 381 328 24 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8458 PsiI TTA'TAA Cuts at: 6028 6028 Size: 8458 PspOMI G'GGCC_C Cuts at: 2262 2615 5403 5692 2262 Size: 353 2788 289 5028 Fragments arranged by size: 5028 2788 353 289 PstI C_TGCA'G Cuts at: 830 2560 2628 2670 4146 5810 830 Size: 1730 68 42 1476 1664 3478 Fragments arranged by size: 3478 1730 1664 1476 68 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8458 PvuII CAG'CTG Cuts at: 4588 4689 4588 Size: 101 8357 SacI G_AGCT'C Cuts at: 3579 5477 5600 3579 Size: 1898 123 6437 Fragments arranged by size: 6437 1898 123 SalI G'TCGA_C Cuts at: 1936 5800 1936 Size: 3864 4594 SanDI GG'GwC_CC Cuts at: 2046 2755 3186 2046 Size: 709 431 7318 Fragments arranged by size: 7318 709 431 SapI GCTCTTCn'nnn_ Cuts at: 3567 3731 5279 5333 5655 3567 Size: 164 1548 54 322 6370 Fragments arranged by size: 6370 1548 322 164 54 SbfI CC_TGCA'GG Cuts at: 5810 5810 Size: 8458 ScaI AGT'ACT Cuts at: 6706 8418 6706 Size: 1712 6746 SexAI A'CCwGG_T Cuts at: 4760 4760 Size: 8458 SfcI C'TryA_G Cuts at: 826 1045 2556 2565 2624 2666 4142 4402 5806 Size: 219 1511 9 59 42 1476 260 1404 Cuts at: 5806 6941 7622 7813 826 Size: 1135 681 191 1471 Fragments arranged by size: 1511 1476 1471 1404 1135 681 260 219 191 59 42 9 SfiI GGCCn_nnn'nGGCC Cuts at: 2447 3671 2447 Size: 1224 7234 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8458 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8458 SmaI CCC'GGG Cuts at: 1872 2633 5309 5583 5691 5785 1872 Size: 761 2676 274 108 94 4545 Fragments arranged by size: 4545 2676 761 274 108 94 SmlI C'TyrA_G Cuts at: 820 1017 1943 2444 3578 6562 7463 7725 820 Size: 197 926 501 1134 2984 901 262 1553 Fragments arranged by size: 2984 1553 1134 926 901 501 262 197 SnaBI TAC'GTA Cuts at: 493 6249 493 Size: 5756 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8458 SphI G_CATG'C Cuts at: 1132 4795 5254 5816 1132 Size: 3663 459 562 3774 Fragments arranged by size: 3774 3663 562 459 SrfI GCCC'GGGC Cuts at: 5691 5691 Size: 8458 SspI AAT'ATT Cuts at: 5 52 6382 5 Size: 47 6330 2081 Fragments arranged by size: 6330 2081 47 StuI AGG'CCT Cuts at: 1517 2464 1517 Size: 947 7511 StyI C'CwwG_G Cuts at: 513 1065 2226 2673 2685 3267 3333 4245 5274 Size: 552 1161 447 12 582 66 912 1029 Cuts at: 5274 5617 5897 513 Size: 343 280 3074 Fragments arranged by size: 3074 1161 1029 912 582 552 447 343 280 66 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2671 3411 5351 5544 6485 6502 6655 Size: 1255 1091 740 1940 193 941 17 153 Cuts at: 6655 6840 7285 325 Size: 185 445 1498 Fragments arranged by size: 1940 1498 1255 1091 941 740 445 193 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4308 4368 4710 Size: 274 80 33 51 1443 2331 60 342 Cuts at: 4710 4770 5001 6704 8416 96 Size: 60 231 1703 1712 138 Fragments arranged by size: 2331 1712 1703 1443 342 274 231 138 80 60 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2402 2434 2886 2918 8418 8450 2402 Size: 32 452 32 5500 32 2410 Fragments arranged by size: 5500 2410 452 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2149 2915 1316 Size: 833 766 6859 Fragments arranged by size: 6859 833 766 XbaI T'CTAG_A Cuts at: 5794 5794 Size: 8458 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3612 3612 Size: 8458 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8458 XmaI C'CCGG_G Cuts at: 1870 2631 5307 5581 5689 5783 1870 Size: 761 2676 274 108 94 4545 Fragments arranged by size: 4545 2676 761 274 108 94 XmnI GAAnn'nnTTC Cuts at: 3725 4598 6587 3725 Size: 873 1989 5596 Fragments arranged by size: 5596 1989 873 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3775 5485 6266 276 Size: 53 83 186 936 2241 1710 781 2468 Fragments arranged by size: 2468 2241 1710 936 781 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfoI SwaI