Restriction map for
pFN21AB7302
(Linear) (Six-base) MAPSORT of: KIBB7302.seq Check: 5561 from: 1 to: 3753
KIBB7302.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1140 1392 3753
Size: 1140 252 2361
Fragments arranged by size:
2361 1140 252
AatII G_ACGT'C
Cuts at: 0 1772 3482 3753
Size: 1772 1710 271
Fragments arranged by size:
1772 1710 271
AccI GT'mk_AC
Cuts at: 0 2945 3753
Size: 2945 808
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1184 1271 1307 1586 1868 2218 2944 3753
Size: 1184 87 36 279 282 350 726 809
Fragments arranged by size:
1184 809 726 350 282 279 87 36
AflIII A'CryG_T
Cuts at: 0 2360 3753
Size: 2360 1393
AlwNI CAG_nnn'CTG
Cuts at: 0 384 495 568 1212 1710 2586 3711 3753
Size: 384 111 73 644 498 876 1125 42
Fragments arranged by size:
1125 876 644 498 384 111 73 42
ApaI G_GGCC'C
Cuts at: 0 261 614 3402 3691 3753
Size: 261 353 2788 289 62
Fragments arranged by size:
2788 353 289 261 62
ApaLI G'TGCA_C
Cuts at: 0 414 3753
Size: 414 3339
ApoI r'AATT_y
Cuts at: 0 2412 2712 3753
Size: 2412 300 1041
Fragments arranged by size:
2412 1041 300
AvaI C'yCGr_G
Cuts at: 0 626 1521 3302 3568 3576 3684 3753
Size: 626 895 1781 266 8 108 69
Fragments arranged by size:
1781 895 626 266 108 69 8
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 968 1001 3753
Size: 968 33 2752
Fragments arranged by size:
2752 968 33
BanI G'GyrC_C
Cuts at: 0 687 3310 3753
Size: 687 2623 443
Fragments arranged by size:
2623 687 443
BanII G_rGCy'C
Cuts at: 0 261 614 704 1310 1574 1792 3402 3472
Size: 261 353 90 606 264 218 1610 70
Cuts at: 3472 3575 3595 3653 3685 3691 3753
Size: 103 20 58 32 6 62
Fragments arranged by size:
1610 606 353 264 261 218 103 90
70 62 58 32 20 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 58 1181 2527 2568 3753
Size: 58 1123 1346 41 1185
Fragments arranged by size:
1346 1185 1123 58 41
BbvCI CC'TCA_GC
Cuts at: 0 29 512 1346 2347 3753
Size: 29 483 834 1001 1406
Fragments arranged by size:
1406 1001 834 483 29
BglI GCCn_nnn'nGGC
Cuts at: 0 442 1666 3753
Size: 442 1224 2087
Fragments arranged by size:
2087 1224 442
BlpI GC'TnA_GC
Cuts at: 0 1515 2227 3753
Size: 1515 712 1526
Fragments arranged by size:
1526 1515 712
Bme1580I G_kGCm'C
Cuts at: 0 261 418 614 3313 3402 3691 3753
Size: 261 157 196 2699 89 289 62
Fragments arranged by size:
2699 289 261 196 157 89 62
BmgBI CAC'GTC
Cuts at: 0 2853 3753
Size: 2853 900
BmrI ACTGGGnnnn_n'
Cuts at: 0 410 1326 1594 2439 2475 3753
Size: 410 916 268 845 36 1278
Fragments arranged by size:
1278 916 845 410 268 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 654 861 3513 3753
Size: 654 207 2652 240
Fragments arranged by size:
2652 654 240 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 558 572 680 808 908 1687 1814
Size: 505 53 14 108 128 100 779 127
Cuts at: 1814 3445 3753
Size: 1631 308
Fragments arranged by size:
1631 779 505 308 128 127 108 100
53 14
Bpu10I CC'TnA_GC
Cuts at: 0 29 512 1346 2347 3753
Size: 29 483 834 1001 1406
Fragments arranged by size:
1406 1001 834 483 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1558 3753
Size: 460 1098 2195
Fragments arranged by size:
2195 1098 460
BsaI GGTCTCn'nnnn_
Cuts at: 0 211 625 1318 3587 3753
Size: 211 414 693 2269 166
Fragments arranged by size:
2269 693 414 211 166
BsaAI yAC'GTr
Cuts at: 0 2361 3753
Size: 2361 1392
BsaBI GATnn'nnATC
Cuts at: 0 2998 3753
Size: 2998 755
BsaHI Gr'CG_yC
Cuts at: 0 594 1769 2603 3479 3562 3753
Size: 594 1175 834 876 83 191
Fragments arranged by size:
1175 876 834 594 191 83
BsaWI w'CCGG_w
Cuts at: 0 3227 3753
Size: 3227 526
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 881 911 3753
Size: 881 30 2842
Fragments arranged by size:
2842 881 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 196 720 934 1069 1084 1087 1102
Size: 145 51 524 214 135 15 3 15
Cuts at: 1102 1105 1108 1111 1120 1123 1246 1282 1291
Size: 3 3 3 9 3 123 36 9
Cuts at: 1291 1306 1348 1798 1882 2269 3289 3646 3649
Size: 15 42 450 84 387 1020 357 3
Cuts at: 3649 3753
Size: 104
Fragments arranged by size:
1020 524 450 387 357 214 145 135
123 104 84 51 42 36 15 15
15 9 9 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 8 292 579 704 1803 2030 3753
Size: 8 284 287 125 1099 227 1723
Fragments arranged by size:
1723 1099 287 284 227 125 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 886 1420 2758 2833 3040 3753
Size: 763 123 534 1338 75 207 713
Fragments arranged by size:
1338 763 713 534 207 123 75
BsiEI CG_ry'CG
Cuts at: 0 1698 2988 3647 3753
Size: 1698 1290 659 106
Fragments arranged by size:
1698 1290 659 106
BsiHKAI G_wGCw'C
Cuts at: 0 418 1198 1574 1874 3472 3595 3662 3753
Size: 418 780 376 300 1598 123 67 91
Fragments arranged by size:
1598 780 418 376 300 123 91 67
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1484 3753
Size: 1484 2269
Bsp1286I G_dGCh'C
Cuts at: 0 261 418 614 704 1198 1310 1574 1792
Size: 261 157 196 90 494 112 264 218
Cuts at: 1792 1874 3313 3402 3472 3575 3595 3653 3662
Size: 82 1439 89 70 103 20 58 9
Cuts at: 3662 3685 3691 3753
Size: 23 6 62
Fragments arranged by size:
1439 494 264 261 218 196 157 112
103 90 89 82 70 62 58 23
20 9 6
BspEI T'CCGG_A
Cuts at: 0 3227 3753
Size: 3227 526
BspHI T'CATG_A
Cuts at: 0 317 3753
Size: 317 3436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1140 1392 2231 2957 3012 3753
Size: 1140 252 839 726 55 741
Fragments arranged by size:
1140 839 741 726 252 55
BsrBI CCG'CTC
Cuts at: 0 1731 3753
Size: 1731 2022
BsrFI r'CCGG_y
Cuts at: 0 1697 2900 3119 3642 3753
Size: 1697 1203 219 523 111
Fragments arranged by size:
1697 1203 523 219 111
BsrGI T'GTAC_A
Cuts at: 0 2363 2996 3753
Size: 2363 633 757
Fragments arranged by size:
2363 757 633
BstAPI GCAn_nnn'nTGC
Cuts at: 0 810 2787 3753
Size: 810 1977 966
Fragments arranged by size:
1977 966 810
BstXI CCAn_nnnn'nTGG
Cuts at: 0 765 3138 3753
Size: 765 2373 615
Fragments arranged by size:
2373 765 615
BstYI r'GATC_y
Cuts at: 0 380 2723 3073 3671 3707 3753
Size: 380 2343 350 598 36 46
Fragments arranged by size:
2343 598 380 350 46 36
Bsu36I CC'TnA_GG
Cuts at: 0 128 212 1340 3753
Size: 128 84 1128 2413
Fragments arranged by size:
2413 1128 128 84
BtgI C'CryG_G
Cuts at: 0 1664 3753
Size: 1664 2089
DraI TTT'AAA
Cuts at: 0 2300 3753
Size: 2300 1453
DraIII CAC_nnn'GTG
Cuts at: 0 413 3666 3753
Size: 413 3253 87
Fragments arranged by size:
3253 413 87
DrdI GACnn_nn'nnGTC
Cuts at: 0 1768 3753
Size: 1768 1985
EaeI y'GGCC_r
Cuts at: 0 1644 1667 2985 3128 3416 3644 3753
Size: 1644 23 1318 143 288 228 109
Fragments arranged by size:
1644 1318 288 228 143 109 23
EagI C'GGCC_G
Cuts at: 0 2985 3644 3753
Size: 2985 659 109
Fragments arranged by size:
2985 659 109
EarI CTCTTCn'nnn_
Cuts at: 0 65 161 998 1046 1073 1079 1097 1247
Size: 65 96 837 48 27 6 18 150
Cuts at: 1247 1283 1439 1562 1726 1859 2920 3166 3274
Size: 36 156 123 164 133 1061 246 108
Cuts at: 3274 3328 3650 3753
Size: 54 322 103
Fragments arranged by size:
1061 837 322 246 164 156 150 133
123 108 103 96 65 54 48 36
27 18 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 118 1336 2272 3489 3753
Size: 118 1218 936 1217 264
Fragments arranged by size:
1218 1217 936 264 118
EcoICRI GAG'CTC
Cuts at: 0 1572 3470 3593 3753
Size: 1572 1898 123 160
Fragments arranged by size:
1898 1572 160 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 558 572 680 808 908 1184 1271
Size: 505 53 14 108 128 100 276 87
Cuts at: 1271 1307 1586 1687 1814 1868 2218 2944 3445
Size: 36 279 101 127 54 350 726 501
Cuts at: 3445 3753
Size: 308
Fragments arranged by size:
726 505 501 350 308 279 276 128
127 108 101 100 87 54 53 36
14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 919 3753
Size: 919 2834
EcoO109I rG'GnC_Cy
Cuts at: 0 41 369 435 610 750 774 1181 2448
Size: 41 328 66 175 140 24 407 1267
Cuts at: 2448 3180 3753
Size: 732 573
Fragments arranged by size:
1267 732 573 407 328 175 140 66
41 24
EcoRI G'AATT_C
Cuts at: 0 2412 3753
Size: 2412 1341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2580 3753
Size: 2580 1173
FseI GG_CCGG'CC
Cuts at: 0 3646 3753
Size: 3646 107
HaeII r_GCGC'y
Cuts at: 0 269 586 1685 3753
Size: 269 317 1099 2068
Fragments arranged by size:
2068 1099 317 269
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 136 162 168 1759 1791 3031 3063
Size: 130 6 26 6 1591 32 1240 32
Cuts at: 3063 3692 3724 3753
Size: 629 32 29
Fragments arranged by size:
1591 1240 629 130 32 32 32 29
26 6 6
HindIII A'AGCT_T
Cuts at: 0 3093 3753
Size: 3093 660
MscI TGG'CCA
Cuts at: 0 1646 3753
Size: 1646 2107
MslI CAynn'nnrTG
Cuts at: 0 953 2119 2851 3753
Size: 953 1166 732 902
Fragments arranged by size:
1166 953 902 732
MspA1I CmG'CkG
Cuts at: 0 1846 1950 2294 2583 2684 3753
Size: 1846 104 344 289 101 1069
Fragments arranged by size:
1846 1069 344 289 104 101
NaeI GCC'GGC
Cuts at: 0 3644 3753
Size: 3644 109
NgoMIV G'CCGG_C
Cuts at: 0 3642 3753
Size: 3642 111
NspI r_CATG'y
Cuts at: 0 1887 2184 2790 2922 3249 3753
Size: 1887 297 606 132 327 504
Fragments arranged by size:
1887 606 504 327 297 132
PasI CC'CwG_GG
Cuts at: 0 37 261 850 935 3753
Size: 37 224 589 85 2818
Fragments arranged by size:
2818 589 224 85 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 606 1710 2465 3753
Size: 606 1104 755 1288
Fragments arranged by size:
1288 1104 755 606
PfoI T'CCnGG_A
Cuts at: 0 588 885 1042 1703 2108 3167 3753
Size: 588 297 157 661 405 1059 586
Fragments arranged by size:
1059 661 588 586 405 297 157
PpuMI rG'GwC_Cy
Cuts at: 0 41 369 750 774 1181 3180 3753
Size: 41 328 381 24 407 1999 573
Fragments arranged by size:
1999 573 407 381 328 41 24
PspOMI G'GGCC_C
Cuts at: 0 257 610 3398 3687 3753
Size: 257 353 2788 289 66
Fragments arranged by size:
2788 353 289 257 66
PstI C_TGCA'G
Cuts at: 0 555 623 665 2141 3753
Size: 555 68 42 1476 1612
Fragments arranged by size:
1612 1476 555 68 42
PvuII CAG'CTG
Cuts at: 0 2583 2684 3753
Size: 2583 101 1069
Fragments arranged by size:
2583 1069 101
SacI G_AGCT'C
Cuts at: 0 1574 3472 3595 3753
Size: 1574 1898 123 158
Fragments arranged by size:
1898 1574 158 123
SanDI GG'GwC_CC
Cuts at: 0 41 750 1181 3753
Size: 41 709 431 2572
Fragments arranged by size:
2572 709 431 41
SapI GCTCTTCn'nnn_
Cuts at: 0 1562 1726 3274 3328 3650 3753
Size: 1562 164 1548 54 322 103
Fragments arranged by size:
1562 1548 322 164 103 54
SexAI A'CCwGG_T
Cuts at: 0 2755 3753
Size: 2755 998
SfcI C'TryA_G
Cuts at: 0 551 560 619 661 2137 2397 3753
Size: 551 9 59 42 1476 260 1356
Fragments arranged by size:
1476 1356 551 260 59 42 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 442 1666 3753
Size: 442 1224 2087
Fragments arranged by size:
2087 1224 442
SmaI CCC'GGG
Cuts at: 0 628 3304 3578 3686 3753
Size: 628 2676 274 108 67
Fragments arranged by size:
2676 628 274 108 67
SmlI C'TyrA_G
Cuts at: 0 439 1573 3753
Size: 439 1134 2180
Fragments arranged by size:
2180 1134 439
SphI G_CATG'C
Cuts at: 0 2790 3249 3753
Size: 2790 459 504
Fragments arranged by size:
2790 504 459
SrfI GCCC'GGGC
Cuts at: 0 3686 3753
Size: 3686 67
StuI AGG'CCT
Cuts at: 0 459 3753
Size: 459 3294
StyI C'CwwG_G
Cuts at: 0 221 668 680 1262 1328 2240 3269 3612
Size: 221 447 12 582 66 912 1029 343
Cuts at: 3612 3753
Size: 141
Fragments arranged by size:
1029 912 582 447 343 221 141 66
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 666 1406 3346 3539 3753
Size: 666 740 1940 193 214
Fragments arranged by size:
1940 740 666 214 193
TatI w'GTAC_w
Cuts at: 0 2303 2363 2705 2765 2996 3753
Size: 2303 60 342 60 231 757
Fragments arranged by size:
2303 757 342 231 60 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 397 429 881 913 3753
Size: 397 32 452 32 2840
Fragments arranged by size:
2840 452 397 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 144 910 3753
Size: 144 766 2843
Fragments arranged by size:
2843 766 144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1607 3753
Size: 1607 2146
XmaI C'CCGG_G
Cuts at: 0 626 3302 3576 3684 3753
Size: 626 2676 274 108 69
Fragments arranged by size:
2676 626 274 108 69
XmnI GAAnn'nnTTC
Cuts at: 0 1720 2593 3753
Size: 1720 873 1160
Fragments arranged by size:
1720 1160 873
ZraI GAC'GTC
Cuts at: 0 1770 3480 3753
Size: 1770 1710 273
Fragments arranged by size:
1770 1710 273
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlwNI ApaI ApaLI
ApoI AvaI BaeI BanI BanII BbsI BbvCI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII Hin4I
HindIII MscI MslI MspA1I NaeI NgoMIV NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI
SapI SexAI SfcI SfiI SmaI SmlI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AseI AsiSI AvrII BamHI BbeI BcgI
BciVI BclI BfrBI BglII BmtI BsiWI BsmI BsrDI
BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI
CspCI EcoRV FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MmeI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SfoI SgfI SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI