(Circular) (Six-base) MAPSORT of: pFN21AB7295.seq Check: 4387 from: 1 to: 7372 pFN21AB7295 7372 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3371 3439 4216 4312 3371 Size: 68 777 96 6431 Fragments arranged by size: 6431 777 96 68 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5182 278 Size: 53 83 186 936 3646 2468 Fragments arranged by size: 3646 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2952 4715 1937 Size: 1015 1763 4594 Fragments arranged by size: 4594 1763 1015 Acc65I G'GTAC_C Cuts at: 4693 4693 Size: 7372 AclI AA'CG_TT Cuts at: 1563 5499 5872 1563 Size: 3936 373 3063 Fragments arranged by size: 3936 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2617 5435 6819 7087 1632 Size: 985 2818 1384 268 1917 Fragments arranged by size: 2818 1917 1384 985 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7175 AflIII A'CryG_T Cuts at: 1203 3462 6271 7248 1203 Size: 2259 2809 977 1327 Fragments arranged by size: 2809 2259 1327 977 AgeI A'CCGG_T Cuts at: 6203 6203 Size: 7372 AhdI GACnn_n'nnGTC Cuts at: 2890 6101 2890 Size: 3211 4161 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7372 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3143 1184 Size: 1959 5413 AlwNI CAG_nnn'CTG Cuts at: 3163 3409 5115 6687 3163 Size: 246 1706 1572 3848 Fragments arranged by size: 3848 1706 1572 246 ApaI G_GGCC'C Cuts at: 2144 2151 2346 2354 2367 2406 2456 3579 4299 Size: 7 195 8 13 39 50 1123 720 Cuts at: 4299 4326 4422 4554 2144 Size: 27 96 132 4962 Fragments arranged by size: 4962 1123 720 195 132 96 50 39 27 13 8 7 ApaLI G'TGCA_C Cuts at: 5429 6585 5429 Size: 1156 6216 ApoI r'AATT_y Cuts at: 1493 4003 4681 4907 5059 7168 1493 Size: 2510 678 226 152 2109 1697 Fragments arranged by size: 2510 2109 1697 678 226 152 AscI GG'CGCG_CC Cuts at: 3952 4350 3952 Size: 398 6974 AseI AT'TA_AT Cuts at: 160 1032 5926 160 Size: 872 4894 1606 Fragments arranged by size: 4894 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7372 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2044 2137 2144 2172 2266 2345 Size: 73 90 11 93 7 28 94 79 Cuts at: 2345 2398 2840 3308 3846 4042 4291 4319 4345 Size: 53 442 468 538 196 249 28 26 Cuts at: 4345 4365 4566 4697 1870 Size: 20 201 131 4545 Fragments arranged by size: 4545 538 468 442 249 201 196 131 94 93 90 79 73 53 28 28 26 20 11 7 BamHI G'GATC_C Cuts at: 4702 5069 4702 Size: 367 7005 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2261 3166 3304 3356 3423 Size: 325 217 627 474 905 138 52 67 Cuts at: 3423 3728 4369 4493 4613 4693 6148 618 Size: 305 641 124 120 80 1455 1842 Fragments arranged by size: 1842 1455 905 641 627 474 325 305 217 138 124 120 80 67 52 BanII G_rGCy'C Cuts at: 1391 2045 2144 2151 2346 2354 2367 2406 2456 Size: 654 99 7 195 8 13 39 50 Cuts at: 2456 3570 3579 4299 4326 4422 4554 4691 1391 Size: 1114 9 720 27 96 132 137 4072 Fragments arranged by size: 4072 1114 720 654 195 137 132 99 96 50 39 27 13 9 8 7 BbeI G_GCGC'C Cuts at: 3308 3427 4497 4617 3308 Size: 119 1070 120 6063 Fragments arranged by size: 6063 1070 120 119 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3688 928 Size: 971 1789 4612 Fragments arranged by size: 4612 1789 971 BbvCI CC'TCA_GC Cuts at: 2999 2999 Size: 7372 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2791 2825 2857 2891 5563 5597 1903 Size: 34 854 34 32 34 2672 34 3678 Fragments arranged by size: 3678 2672 854 34 34 34 34 32 BciVI GTATCCnnnnn_n' Cuts at: 5264 6474 5264 Size: 1210 6162 BclI T'GATC_A Cuts at: 1548 1548 Size: 7372 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2220 2259 2301 Size: 107 122 71 631 605 548 39 42 Cuts at: 2301 2505 4294 4347 5983 136 Size: 204 1789 53 1636 1525 Fragments arranged by size: 1789 1636 1525 631 605 548 204 122 107 71 53 42 39 BglII A'GATC_T Cuts at: 3060 3831 7367 3060 Size: 771 3536 3065 Fragments arranged by size: 3536 3065 771 BlpI GC'TnA_GC Cuts at: 3341 3902 4789 3341 Size: 561 887 5924 Fragments arranged by size: 5924 887 561 Bme1580I G_kGCm'C Cuts at: 1790 2144 2151 2346 2354 2367 2406 2456 3579 Size: 354 7 195 8 13 39 50 1123 Cuts at: 3579 4299 4326 4372 4422 4554 5433 6589 1790 Size: 720 27 46 50 132 879 1156 2573 Fragments arranged by size: 2573 1156 1123 879 720 354 195 132 50 50 46 39 27 13 8 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7372 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2653 2687 3151 Size: 416 53 83 399 24 1236 34 464 Cuts at: 3151 5103 6061 442 Size: 1952 958 1753 Fragments arranged by size: 1952 1753 1236 958 464 416 399 83 53 34 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7372 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3950 6032 1239 Size: 2711 2082 2579 Fragments arranged by size: 2711 2579 2082 Bpu10I CC'TnA_GC Cuts at: 2999 4442 2999 Size: 1443 5929 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3668 5497 6362 6660 3668 Size: 1829 865 298 4380 Fragments arranged by size: 4380 1829 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2714 2758 6035 882 Size: 617 1215 44 3277 2219 Fragments arranged by size: 3277 2219 1215 617 44 BsaAI yAC'GTr Cuts at: 493 1204 2871 5163 493 Size: 711 1667 2292 2702 Fragments arranged by size: 2702 2292 1667 711 BsaBI GATnn'nnATC Cuts at: 3101 7366 3101 Size: 4265 3107 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2526 3305 3424 4494 Size: 53 83 186 936 993 779 119 1070 Cuts at: 4494 4614 5179 5561 6196 275 Size: 120 565 382 635 1451 Fragments arranged by size: 1451 1070 993 936 779 635 565 382 186 120 119 83 53 BsaWI w'CCGG_w Cuts at: 5805 6203 6477 6624 7035 5805 Size: 398 274 147 411 6142 Fragments arranged by size: 6142 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7147 Fragments arranged by size: 7147 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 2459 3071 3406 3602 3947 1186 Size: 1258 15 612 335 196 345 4611 Fragments arranged by size: 4611 1258 612 345 335 196 15 BseYI C'CCAG_C Cuts at: 2160 3198 4123 4415 4580 6575 7071 2160 Size: 1038 925 292 165 1995 496 2461 Fragments arranged by size: 2461 1995 1038 925 496 292 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2350 4001 4046 4130 1163 Size: 573 614 1651 45 84 4405 Fragments arranged by size: 4405 1651 614 573 84 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2254 3298 3979 4099 4238 4822 Size: 275 186 253 1044 681 120 139 584 Cuts at: 4822 5583 6611 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1044 1028 761 681 584 275 253 186 139 120 BsiHKAI G_wGCw'C Cuts at: 2282 3522 3570 3780 5433 5518 6589 2282 Size: 1240 48 210 1653 85 1071 3065 Fragments arranged by size: 3065 1653 1240 1071 210 85 48 BsmI GAATG_Cn' Cuts at: 4883 4976 4883 Size: 93 7279 BsmBI CGTCTCn'nnnn_ Cuts at: 3956 3956 Size: 7372 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2144 2151 2282 2346 2354 2367 Size: 399 255 99 7 131 64 8 13 Cuts at: 2367 2406 2456 3522 3570 3579 3780 4299 4326 Size: 39 50 1066 48 9 201 519 27 Cuts at: 4326 4372 4422 4554 4691 5433 5518 6589 1391 Size: 46 50 132 137 742 85 1071 2174 Fragments arranged by size: 2174 1071 1066 742 519 399 255 201 137 132 131 99 85 64 50 50 48 46 39 27 13 9 8 7 BspEI T'CCGG_A Cuts at: 7035 7035 Size: 7372 BspHI T'CATG_A Cuts at: 5261 6991 5261 Size: 1730 5642 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3371 3439 4159 4216 4312 4727 844 Size: 2527 68 720 57 96 415 3489 Fragments arranged by size: 3489 2527 720 415 96 68 57 BsrBI CCG'CTC Cuts at: 1394 1823 4240 5259 1394 Size: 429 2417 1019 3507 Fragments arranged by size: 3507 2417 1019 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5867 6041 65 Size: 1183 4619 174 1396 Fragments arranged by size: 4619 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2223 2422 2798 3297 4619 6016 6203 Size: 110 413 199 376 499 1322 1397 187 Cuts at: 6203 1700 Size: 2869 Fragments arranged by size: 2869 1397 1322 499 413 376 199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7372 BssHII G'CGCG_C Cuts at: 1925 2012 2508 3952 4350 4433 4646 1925 Size: 87 496 1444 398 83 213 4651 Fragments arranged by size: 4651 1444 496 398 213 87 83 BssSI C'ACGA_G Cuts at: 2245 2380 3229 4241 4274 5432 6444 2245 Size: 135 849 1012 33 1158 1012 3173 Fragments arranged by size: 3173 1158 1012 1012 849 135 33 BstAPI GCAn_nnn'nTGC Cuts at: 794 4024 794 Size: 3230 4142 BstBI TT'CG_AA Cuts at: 6176 6176 Size: 7372 BstEII G'GTnAC_C Cuts at: 1184 4171 1184 Size: 2987 4385 BstXI CCAn_nnnn'nTGG Cuts at: 2905 2905 Size: 7372 BstYI r'GATC_y Cuts at: 1972 2994 3060 3102 3501 3831 4702 5069 5454 Size: 1022 66 42 399 330 871 367 385 Cuts at: 5454 5471 6923 7009 7021 7367 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1022 871 399 385 367 346 330 86 66 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2216 2337 2386 2568 2732 2904 Size: 552 939 212 121 49 182 164 172 Cuts at: 2904 4373 4506 513 Size: 1469 133 3379 Fragments arranged by size: 3379 1469 939 552 212 182 172 164 133 121 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4168 487 Size: 683 56 2942 3691 Fragments arranged by size: 3691 2942 683 56 BtsI GCAGTG_nn' Cuts at: 2333 2724 3390 4888 5682 5702 2333 Size: 391 666 1498 794 20 4003 Fragments arranged by size: 4003 1498 794 666 391 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7372 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7337 DraI TTT'AAA Cuts at: 4676 4676 Size: 7372 DraIII CAC_nnn'GTG Cuts at: 3766 4024 3766 Size: 258 7114 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2948 6379 809 Size: 507 1632 3431 1802 Fragments arranged by size: 3431 1802 1632 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2251 2283 3084 Size: 54 1425 50 275 18 421 32 801 Cuts at: 3084 3295 3382 3666 3969 4179 4206 4235 4524 Size: 211 87 284 303 210 27 29 289 Cuts at: 4524 4819 5708 7337 8 Size: 295 889 1629 43 Fragments arranged by size: 1629 1425 889 801 421 303 295 289 284 275 211 210 87 54 50 43 32 29 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2251 3295 4235 4819 1537 Size: 275 439 1044 940 584 4090 Fragments arranged by size: 4090 1044 940 584 439 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3263 4148 5302 1353 Size: 1910 885 1154 3423 Fragments arranged by size: 3423 1910 1154 885 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2216 2441 3482 4456 5959 6333 Size: 1064 138 627 225 1041 974 1503 374 Cuts at: 6333 6479 387 Size: 146 1280 Fragments arranged by size: 1503 1280 1064 1041 974 627 374 225 146 138 EcoICRI GAG'CTC Cuts at: 3568 3568 Size: 7372 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2617 3950 5435 6032 6819 7087 1239 Size: 393 985 1333 1485 597 787 268 1524 Fragments arranged by size: 1524 1485 1333 985 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3584 4019 3584 Size: 435 6937 EcoO109I rG'GnC_Cy Cuts at: 2141 2342 2350 2363 2402 2604 3576 4295 4323 Size: 201 8 13 39 202 972 719 28 Cuts at: 4323 4550 2141 Size: 227 4963 Fragments arranged by size: 4963 972 719 227 202 201 39 28 13 8 EcoRI G'AATT_C Cuts at: 4003 4681 5059 4003 Size: 678 378 6316 Fragments arranged by size: 6316 678 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3766 3766 Size: 7372 FspI TGC'GCA Cuts at: 2867 2975 5878 2867 Size: 108 2903 4361 Fragments arranged by size: 4361 2903 108 HaeII r_GCGC'y Cuts at: 1678 2500 3308 3427 4259 4497 4617 6519 1678 Size: 822 808 119 832 238 120 1902 2531 Fragments arranged by size: 2531 1902 832 822 808 238 120 119 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2876 2908 3094 3126 4025 Size: 32 135 32 1304 32 186 32 899 Cuts at: 4025 4057 4589 4621 6013 6045 6087 6119 1373 Size: 32 532 32 1392 32 42 32 2626 Fragments arranged by size: 2626 1392 1304 899 532 186 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2530 3598 4716 4962 669 Size: 988 281 592 1068 1118 246 3079 Fragments arranged by size: 3079 1118 1068 988 592 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7372 HpaI GTT'AAC Cuts at: 4962 4962 Size: 7372 KasI G'GCGC_C Cuts at: 3304 3423 4493 4613 3304 Size: 119 1070 120 6063 Fragments arranged by size: 6063 1070 120 119 KpnI G_GTAC'C Cuts at: 4697 4697 Size: 7372 MfeI C'AATT_G Cuts at: 4971 4971 Size: 7372 MluI A'CGCG_T Cuts at: 7248 7248 Size: 7372 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4689 6486 6670 1305 Size: 3384 1797 184 2007 Fragments arranged by size: 3384 2007 1797 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3086 3971 10 Size: 54 1425 343 1254 885 3411 Fragments arranged by size: 3411 1425 1254 885 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2693 2903 3119 3350 3668 5330 5689 Size: 648 1527 210 216 231 318 1662 359 Cuts at: 5689 5848 518 Size: 159 2042 Fragments arranged by size: 2042 1662 1527 648 359 318 231 216 210 159 MspA1I CmG'CkG Cuts at: 1606 2218 2339 2388 3485 3634 4252 4375 4508 Size: 612 121 49 1097 149 618 123 133 Cuts at: 4508 4562 4788 5465 6613 6858 1606 Size: 54 226 677 1148 245 2120 Fragments arranged by size: 2120 1148 1097 677 618 612 245 226 149 133 123 121 54 49 NaeI GCC'GGC Cuts at: 2225 2424 3299 4621 2225 Size: 199 875 1322 4976 Fragments arranged by size: 4976 1322 875 199 NarI GG'CG_CC Cuts at: 3305 3424 4494 4614 3305 Size: 119 1070 120 6063 Fragments arranged by size: 6063 1070 120 119 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5881 Fragments arranged by size: 5881 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7372 NgoMIV G'CCGG_C Cuts at: 2223 2422 3297 4619 2223 Size: 199 875 1322 4976 Fragments arranged by size: 4976 1322 875 199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7372 NotI GC'GGCC_GC Cuts at: 2251 4235 4819 2251 Size: 1984 584 4804 Fragments arranged by size: 4804 1984 584 NspI r_CATG'y Cuts at: 1132 1229 3280 3466 4730 6275 1132 Size: 97 2051 186 1264 1545 2229 Fragments arranged by size: 2229 2051 1545 1264 186 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7372 PciI A'CATG_T Cuts at: 3462 6271 3462 Size: 2809 4563 PflMI CCAn_nnn'nTGG Cuts at: 1108 3409 1108 Size: 2301 5071 PfoI T'CCnGG_A Cuts at: 2832 4077 2832 Size: 1245 6127 PmeI GTTT'AAAC Cuts at: 4676 4676 Size: 7372 PmlI CAC'GTG Cuts at: 2871 2871 Size: 7372 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5441 5473 6954 6986 5441 Size: 32 1481 32 5827 Fragments arranged by size: 5827 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7372 PsiI TTA'TAA Cuts at: 4942 4942 Size: 7372 PspOMI G'GGCC_C Cuts at: 2140 2147 2342 2350 2363 2402 2452 3575 4295 Size: 7 195 8 13 39 50 1123 720 Cuts at: 4295 4322 4418 4550 2140 Size: 27 96 132 4962 Fragments arranged by size: 4962 1123 720 195 132 96 50 39 27 13 8 7 PstI C_TGCA'G Cuts at: 830 2520 3159 3180 4197 4724 830 Size: 1690 639 21 1017 527 3478 Fragments arranged by size: 3478 1690 1017 639 527 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7372 PvuII CAG'CTG Cuts at: 3485 3485 Size: 7372 RsrII CG'GwC_CG Cuts at: 4588 4588 Size: 7372 SacI G_AGCT'C Cuts at: 3570 3570 Size: 7372 SacII CC_GC'GG Cuts at: 2219 2340 2389 4376 4509 2219 Size: 121 49 1987 133 5082 Fragments arranged by size: 5082 1987 133 121 49 SalI G'TCGA_C Cuts at: 1936 4714 1936 Size: 2778 4594 SbfI CC_TGCA'GG Cuts at: 4724 4724 Size: 7372 ScaI AGT'ACT Cuts at: 5620 7332 5620 Size: 1712 5660 SexAI A'CCwGG_T Cuts at: 2778 2778 Size: 7372 SfcI C'TryA_G Cuts at: 826 1045 2516 3155 3176 4193 4720 5855 6536 Size: 219 1471 639 21 1017 527 1135 681 Cuts at: 6536 6727 826 Size: 191 1471 Fragments arranged by size: 1471 1471 1135 1017 681 639 527 219 191 21 SfiI GGCCn_nnn'nGGCC Cuts at: 2220 2220 Size: 7372 SfoI GGC'GCC Cuts at: 3306 3425 4495 4615 3306 Size: 119 1070 120 6063 Fragments arranged by size: 6063 1070 120 119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7372 SgrAI Cr'CCGG_yG Cuts at: 1700 4619 1700 Size: 2919 4453 SmaI CCC'GGG Cuts at: 1872 2035 2046 2139 2146 2174 2268 3848 4293 Size: 163 11 93 7 28 94 1580 445 Cuts at: 4293 4321 4347 4367 4568 4699 1872 Size: 28 26 20 201 131 4545 Fragments arranged by size: 4545 1580 445 201 163 131 94 93 28 28 26 20 11 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 3683 5476 6377 6639 820 Size: 197 926 1740 1793 901 262 1553 Fragments arranged by size: 1793 1740 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5163 493 Size: 4670 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7372 SphI G_CATG'C Cuts at: 1132 4730 1132 Size: 3598 3774 SrfI GCCC'GGGC Cuts at: 2046 4568 2046 Size: 2522 4850 SspI AAT'ATT Cuts at: 5 52 5296 5 Size: 47 5244 2081 Fragments arranged by size: 5244 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7372 StyI C'CwwG_G Cuts at: 513 1065 2004 2286 2367 4811 513 Size: 552 939 282 81 2444 3074 Fragments arranged by size: 3074 2444 939 552 282 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3190 3344 5399 5416 5569 5754 6199 Size: 1255 1610 154 2055 17 153 185 445 Cuts at: 6199 325 Size: 1498 Fragments arranged by size: 2055 1610 1498 1255 445 185 154 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3243 3441 3459 Size: 274 80 33 51 1443 1266 198 18 Cuts at: 3459 3747 5618 7330 96 Size: 288 1871 1712 138 Fragments arranged by size: 1871 1712 1443 1266 288 274 198 138 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3018 3050 7332 7364 3018 Size: 32 4282 32 3026 Fragments arranged by size: 4282 3026 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7372 XbaI T'CTAG_A Cuts at: 4708 4708 Size: 7372 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3214 3979 4543 3214 Size: 765 564 6043 Fragments arranged by size: 6043 765 564 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7372 XmaI C'CCGG_G Cuts at: 1870 2033 2044 2137 2144 2172 2266 3846 4291 Size: 163 11 93 7 28 94 1580 445 Cuts at: 4291 4319 4345 4365 4566 4697 1870 Size: 28 26 20 201 131 4545 Fragments arranged by size: 4545 1580 445 201 163 131 94 93 28 28 26 20 11 7 XmnI GAAnn'nnTTC Cuts at: 5501 5501 Size: 7372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5180 276 Size: 53 83 186 936 3646 2468 Fragments arranged by size: 3646 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI SanDI SapI SwaI