################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BcnI BfrI BglI BglII BlnI BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI HaeII Hin1I HpaI KasI KpnI KspI MaeII MfeI MluI MroI MunI MvnI NaeI NarI NciI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AocI 22 CCTCAGG CC^T[ACGT]AGG AseI 831 ATTAAT AT^TAAT AsnI 831 ATTAAT AT^TAAT Asp700I 1680 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BcgI 535 GCAATGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 581 GCTTAGC GC^T[ACGT]AGC Bpu1102I 581 GCTTAGC GC^T[ACGT]AGC BsaBI 1570 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1462 GGTCTC GGTCTC(1/5) BsmBI 1831 CGTCTC CGTCTC(1/5) BstXI 2039 CCATCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 22 CCTCAGG CC^T[ACGT]AGG CelII 581 GCTTAGC GC^T[ACGT]AGC DraII 1278 AGGCCCC [GA]G^G[ACGT]CC[CT] Eco81I 22 CCTCAGG CC^T[ACGT]AGG EcoO109I 1278 AGGCCCC [GA]G^G[ACGT]CC[CT] HapII 475 CCGG C^CGG HincII 427 GTTGAC GT[CT]^[GA]AC HindII 427 GTTGAC GT[CT]^[GA]AC HindIII 526 AAGCTT A^AGCTT HpaII 475 CCGG C^CGG MamI 1570 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 475 CCGG C^CGG NdeI 1246 CATATG CA^TATG NheI 1035 GCTAGC G^CTAGC PflMI 2104 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 831 ATTAAT AT^TAAT SacI 457 GAGCTC GAGCT^C SapI 454 GAAGAGC GCTCTTC(1/4) ScaI 1973 AGTACT AGT^ACT SmlI 2085 CTCAAG C^T[CT][GA]AG Van91I 2104 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1083 CCAAGGCAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1680 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaI 574 CCCGAG C^[CT]CG[GA]G 1637 CCCGAG AviII 1919 TGCGCA TGC^GCA 1946 TGCGCA BalI 234 TGGCCA TGG^CCA 719 TGGCCA BanII 136 GGGCTC G[GA]GC[CT]^C 457 GAGCTC BbsI 42 GTCTTC GAAGAC(2/6) 294 GTCTTC BclI 614 TGATCA T^GATCA 855 TGATCA BpuAI 42 GTCTTC GAAGAC(2/6) 294 GTCTTC BsmFI 568 GGGAC GGGAC(10/14) 1297 GTCCC BsmI 1680 GAATGC GAATGC(1/-1) 1118 GCATTC BsoBI 574 CCCGAG C^[CT]CG[GA]G 1637 CCCGAG BstAPI 970 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1959 GCAGGAAATGC Cfr13I 1000 GGCCC G^G[ACGT]CC 1279 GGCCC EaeI 234 TGGCCA [CT]^GGCC[GA] 719 TGGCCA FbaI 614 TGATCA T^GATCA 855 TGATCA FspI 1919 TGCGCA TGC^GCA 1946 TGCGCA MluNI 234 TGGCCA TGG^CCA 719 TGGCCA MscI 234 TGGCCA TGG^CCA 719 TGGCCA MslI 606 CATGGGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2040 CATCGCCATG NspI 162 GCATGT [GA]CATG^[CT] 1739 ACATGC PstI 872 CTGCAG CTGCA^G 1012 CTGCAG Sau96I 1000 GGCCC G^G[ACGT]CC 1279 GGCCC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI DdeI DpnII DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HgaI HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NcoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII