(Circular) (Six-base) MAPSORT of: pFN21AB6257.seq Check: 2586 from: 1 to: 6994 pFN21AB6257 6994 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3908 4083 3908 Size: 175 6819 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4804 278 Size: 53 83 186 936 3268 2468 Fragments arranged by size: 3268 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4337 1937 Size: 2400 4594 Acc65I G'GTAC_C Cuts at: 4315 4315 Size: 6994 AclI AA'CG_TT Cuts at: 1563 4293 5121 5494 1563 Size: 2730 828 373 3063 Fragments arranged by size: 3063 2730 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2899 3309 5057 6441 6709 1632 Size: 1267 410 1748 1384 268 1917 Fragments arranged by size: 1917 1748 1384 1267 410 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6797 AflIII A'CryG_T Cuts at: 1203 5893 6870 1203 Size: 4690 977 1327 Fragments arranged by size: 4690 1327 977 AgeI A'CCGG_T Cuts at: 5825 5825 Size: 6994 AhdI GACnn_n'nnGTC Cuts at: 5723 5723 Size: 6994 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6994 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3045 3938 1184 Size: 1861 893 4240 Fragments arranged by size: 4240 1861 893 AlwNI CAG_nnn'CTG Cuts at: 2981 3187 3526 3853 4115 4737 6309 2981 Size: 206 339 327 262 622 1572 3666 Fragments arranged by size: 3666 1572 622 339 327 262 206 ApaLI G'TGCA_C Cuts at: 5051 6207 5051 Size: 1156 5838 ApoI r'AATT_y Cuts at: 1493 2201 3554 3627 4206 4303 4529 4681 6790 Size: 708 1353 73 579 97 226 152 2109 Cuts at: 6790 1493 Size: 1697 Fragments arranged by size: 2109 1697 1353 708 579 226 152 97 73 AseI AT'TA_AT Cuts at: 160 1032 2837 5548 160 Size: 872 1805 2711 1606 Fragments arranged by size: 2711 1805 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6994 AvaI C'yCGr_G Cuts at: 1870 1943 2579 3642 4319 1870 Size: 73 636 1063 677 4545 Fragments arranged by size: 4545 1063 677 636 73 BamHI G'GATC_C Cuts at: 4324 4691 4324 Size: 367 6627 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4315 5770 618 Size: 325 217 627 2528 1455 1842 Fragments arranged by size: 2528 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2145 2466 4313 1391 Size: 754 321 1847 4072 Fragments arranged by size: 4072 1847 754 321 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2040 2292 928 Size: 971 141 252 5630 Fragments arranged by size: 5630 971 252 141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2529 2563 5185 5219 1903 Size: 34 50 34 508 34 2622 34 3678 Fragments arranged by size: 3678 2622 508 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4886 6096 4886 Size: 1210 5784 BclI T'GATC_A Cuts at: 1548 2619 2860 1548 Size: 1071 241 5682 Fragments arranged by size: 5682 1071 241 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5605 136 Size: 107 122 71 631 605 3933 1525 Fragments arranged by size: 3933 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6989 6989 Size: 6994 BlpI GC'TnA_GC Cuts at: 2587 4411 2587 Size: 1824 5170 Bme1580I G_kGCm'C Cuts at: 1790 5055 6211 1790 Size: 3265 1156 2573 Fragments arranged by size: 3265 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6994 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2487 4725 5683 Size: 416 53 83 399 24 1070 2238 958 Cuts at: 5683 442 Size: 1753 Fragments arranged by size: 2238 1753 1070 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3044 1056 Size: 1988 5006 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3534 3534 Size: 6994 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2783 2892 3045 3527 3630 3875 4059 5654 Size: 1544 109 153 482 103 245 184 1595 Cuts at: 5654 1239 Size: 2579 Fragments arranged by size: 2579 1595 1544 482 245 184 153 109 103 Bpu10I CC'TnA_GC Cuts at: 3001 3001 Size: 6994 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 5119 5984 6282 4075 Size: 1044 865 298 4787 Fragments arranged by size: 4787 1044 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3473 5657 882 Size: 617 1974 2184 2219 Fragments arranged by size: 2219 2184 1974 617 BsaAI yAC'GTr Cuts at: 493 1204 4785 493 Size: 711 3581 2702 Fragments arranged by size: 3581 2702 711 BsaBI GATnn'nnATC Cuts at: 3579 6988 3579 Size: 3409 3585 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4801 5183 5818 275 Size: 53 83 186 936 3268 382 635 1451 Fragments arranged by size: 3268 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5427 5825 6099 6246 6657 5427 Size: 398 274 147 411 5764 Fragments arranged by size: 5764 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4249 4279 1178 Size: 30 165 30 2846 30 3893 Fragments arranged by size: 3893 2846 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 2891 3839 4172 1186 Size: 958 747 948 333 4008 Fragments arranged by size: 4008 958 948 747 333 BseYI C'CCAG_C Cuts at: 2146 2886 3036 3304 4055 6197 6693 2146 Size: 740 150 268 751 2142 496 2447 Fragments arranged by size: 2447 2142 751 740 496 268 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6421 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4444 5205 6233 1540 Size: 275 186 2443 761 1028 2301 Fragments arranged by size: 2443 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2466 3143 4089 4139 5055 5140 6211 2466 Size: 677 946 50 916 85 1071 3249 Fragments arranged by size: 3249 1071 946 916 677 85 50 BsmI GAATG_Cn' Cuts at: 3123 3691 4505 4598 3123 Size: 568 814 93 5519 Fragments arranged by size: 5519 814 568 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3842 3842 Size: 6994 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2466 3143 4089 4139 4313 5055 Size: 399 355 321 677 946 50 174 742 Cuts at: 5055 5140 6211 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 946 742 677 399 355 321 174 85 50 BspEI T'CCGG_A Cuts at: 6657 6657 Size: 6994 BspHI T'CATG_A Cuts at: 4883 6613 4883 Size: 1730 5264 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3043 3908 4083 4349 844 Size: 2199 865 175 266 3489 Fragments arranged by size: 3489 2199 865 266 175 BsrBI CCG'CTC Cuts at: 1394 1823 4881 1394 Size: 429 3058 3507 Fragments arranged by size: 3507 3058 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2547 3054 3573 3664 4011 5489 5663 Size: 1183 1299 507 519 91 347 1478 174 Cuts at: 5663 65 Size: 1396 Fragments arranged by size: 1478 1396 1299 1183 519 507 347 174 91 BsrFI r'CCGG_y Cuts at: 1700 1810 5638 5825 1700 Size: 110 3828 187 2869 Fragments arranged by size: 3828 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6994 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6994 BssSI C'ACGA_G Cuts at: 5054 6066 5054 Size: 1012 5982 BstAPI GCAn_nnn'nTGC Cuts at: 794 2981 3970 794 Size: 2187 989 3818 Fragments arranged by size: 3818 2187 989 BstBI TT'CG_AA Cuts at: 5798 5798 Size: 6994 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6994 BstXI CCAn_nnnn'nTGG Cuts at: 4051 4051 Size: 6994 BstYI r'GATC_y Cuts at: 1972 2808 3723 3776 4005 4324 4691 5076 5093 Size: 836 915 53 229 319 367 385 17 Cuts at: 5093 6545 6631 6643 6989 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 915 836 385 367 346 319 229 86 53 17 12 Bsu36I CC'TnA_GG Cuts at: 2028 2028 Size: 6994 BtgI C'CryG_G Cuts at: 513 1065 2097 2970 4050 513 Size: 552 1032 873 1080 3457 Fragments arranged by size: 3457 1080 1032 873 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4030 487 Size: 683 56 2804 3451 Fragments arranged by size: 3451 2804 683 56 BtsI GCAGTG_nn' Cuts at: 4017 4510 5304 5324 4017 Size: 493 794 20 5687 Fragments arranged by size: 5687 794 493 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6994 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3568 3603 529 Size: 35 3004 35 3920 Fragments arranged by size: 3920 3004 35 35 DraI TTT'AAA Cuts at: 4298 4298 Size: 6994 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6001 809 Size: 507 4685 1802 Fragments arranged by size: 4685 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2239 2724 4441 Size: 54 1425 50 275 18 409 485 1717 Cuts at: 4441 5330 6959 8 Size: 889 1629 43 Fragments arranged by size: 1717 1629 1425 889 485 409 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4441 1537 Size: 275 2629 4090 Fragments arranged by size: 4090 2629 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 2454 4170 4224 4924 1353 Size: 768 333 1716 54 700 3423 Fragments arranged by size: 3423 1716 768 700 333 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5581 5955 6101 387 Size: 1064 138 3992 374 146 1280 Fragments arranged by size: 3992 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2464 2464 Size: 6994 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2783 2892 2899 3045 3309 3527 3630 Size: 393 1151 109 7 146 264 218 103 Cuts at: 3630 3875 4059 5057 5654 6441 6709 1239 Size: 245 184 998 597 787 268 1524 Fragments arranged by size: 1524 1151 998 787 597 393 268 264 245 218 184 146 109 103 7 EcoO109I rG'GnC_Cy Cuts at: 3284 3284 Size: 6994 EcoRI G'AATT_C Cuts at: 4303 4681 4303 Size: 378 6616 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2064 2549 3113 2064 Size: 485 564 5945 Fragments arranged by size: 5945 564 485 FspI TGC'GCA Cuts at: 3926 3953 5500 3926 Size: 27 1547 5420 Fragments arranged by size: 5420 1547 27 HaeII r_GCGC'y Cuts at: 1678 6141 1678 Size: 4463 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2360 2392 2886 2918 3209 Size: 32 135 32 788 32 494 32 291 Cuts at: 3209 3241 5635 5667 5709 5741 1373 Size: 32 2394 32 42 32 2626 Fragments arranged by size: 2626 2394 788 494 291 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2434 4338 4584 669 Size: 988 281 496 1904 246 3079 Fragments arranged by size: 3079 1904 988 496 281 246 HindIII A'AGCT_T Cuts at: 748 2531 748 Size: 1783 5211 HpaI GTT'AAC Cuts at: 4584 4584 Size: 6994 KpnI G_GTAC'C Cuts at: 4319 4319 Size: 6994 MfeI C'AATT_G Cuts at: 4593 4593 Size: 6994 MluI A'CGCG_T Cuts at: 6870 6870 Size: 6994 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3037 3439 3780 6108 6292 1305 Size: 1732 402 341 2328 184 2007 Fragments arranged by size: 2328 2007 1732 402 341 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2241 2726 10 Size: 54 1425 343 409 485 4278 Fragments arranged by size: 4278 1425 485 409 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2615 4049 4952 5311 5470 518 Size: 648 1449 1434 903 359 159 2042 Fragments arranged by size: 2042 1449 1434 903 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2629 2650 2827 2882 2956 2978 4410 5087 Size: 1023 21 177 55 74 22 1432 677 Cuts at: 5087 6235 6480 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1432 1148 1023 677 245 177 74 55 22 21 NcoI C'CATG_G Cuts at: 513 1065 2097 2970 4050 513 Size: 552 1032 873 1080 3457 Fragments arranged by size: 3457 1080 1032 873 552 NdeI CA'TA_TG Cuts at: 387 3252 387 Size: 2865 4129 NheI G'CTAG_C Cuts at: 1052 3040 1052 Size: 1988 5006 NotI GC'GGCC_GC Cuts at: 4441 4441 Size: 6994 NspI r_CATG'y Cuts at: 1132 1229 2171 3748 4352 5897 1132 Size: 97 942 1577 604 1545 2229 Fragments arranged by size: 2229 1577 1545 942 604 97 PasI CC'CwG_GG Cuts at: 1348 2389 1348 Size: 1041 5953 PciI A'CATG_T Cuts at: 5893 5893 Size: 6994 PflMI CCAn_nnn'nTGG Cuts at: 1108 4115 1108 Size: 3007 3987 PfoI T'CCnGG_A Cuts at: 3061 3061 Size: 6994 PmeI GTTT'AAAC Cuts at: 4298 4298 Size: 6994 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5063 5095 6576 6608 5063 Size: 32 1481 32 5449 Fragments arranged by size: 5449 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6994 PsiI TTA'TAA Cuts at: 4564 4564 Size: 6994 PstI C_TGCA'G Cuts at: 830 2881 3021 4346 830 Size: 2051 140 1325 3478 Fragments arranged by size: 3478 2051 1325 140 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6994 PvuII CAG'CTG Cuts at: 2629 2650 2827 2882 2956 2978 2629 Size: 21 177 55 74 22 6645 Fragments arranged by size: 6645 177 74 55 22 21 SacI G_AGCT'C Cuts at: 2466 2466 Size: 6994 SalI G'TCGA_C Cuts at: 1936 4336 1936 Size: 2400 4594 SapI GCTCTTCn'nnn_ Cuts at: 2454 2454 Size: 6994 SbfI CC_TGCA'GG Cuts at: 4346 4346 Size: 6994 ScaI AGT'ACT Cuts at: 3980 5242 6954 3980 Size: 1262 1712 4020 Fragments arranged by size: 4020 1712 1262 SfcI C'TryA_G Cuts at: 826 1045 2771 2877 3017 4342 5477 6158 6349 Size: 219 1726 106 140 1325 1135 681 191 Cuts at: 6349 826 Size: 1471 Fragments arranged by size: 1726 1471 1325 1135 681 219 191 140 106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6994 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6994 SmaI CCC'GGG Cuts at: 1872 4321 1872 Size: 2449 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4090 5098 5999 6261 820 Size: 197 926 2147 1008 901 262 1553 Fragments arranged by size: 2147 1553 1008 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4785 493 Size: 4292 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6994 SphI G_CATG'C Cuts at: 1132 4352 1132 Size: 3220 3774 SspI AAT'ATT Cuts at: 5 52 4918 5 Size: 47 4866 2081 Fragments arranged by size: 4866 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6994 StyI C'CwwG_G Cuts at: 513 1065 2097 2970 3088 3097 3375 4050 4433 Size: 552 1032 873 118 9 278 675 383 Cuts at: 4433 513 Size: 3074 Fragments arranged by size: 3074 1032 873 675 552 383 278 118 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3172 3244 3253 5021 5038 5191 5376 Size: 1255 1592 72 9 1768 17 153 185 Cuts at: 5376 5821 325 Size: 445 1498 Fragments arranged by size: 1768 1592 1498 1255 445 185 153 72 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2381 3876 3978 Size: 274 80 33 51 1443 404 1495 102 Cuts at: 3978 5240 6952 96 Size: 1262 1712 138 Fragments arranged by size: 1712 1495 1443 1262 404 274 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4249 4281 6954 6986 4249 Size: 32 2673 32 4257 Fragments arranged by size: 4257 2673 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6994 XbaI T'CTAG_A Cuts at: 4330 4330 Size: 6994 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3095 3095 Size: 6994 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6994 XmaI C'CCGG_G Cuts at: 1870 4319 1870 Size: 2449 4545 XmnI GAAnn'nnTTC Cuts at: 3689 5123 3689 Size: 1434 5560 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4802 276 Size: 53 83 186 936 3268 2468 Fragments arranged by size: 3268 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI