(Linear) (Six-base) MAPSORT of: KIBB6257.seq Check: 9399 from: 1 to: 2289 KIBB6257.orf With 182 enzymes: SgfI * July 3, 2012 16:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1903 2078 2289 Size: 1903 175 211 Fragments arranged by size: 1903 211 175 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 894 1304 2289 Size: 894 410 985 Fragments arranged by size: 985 894 410 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1040 1933 2289 Size: 1040 893 356 Fragments arranged by size: 1040 893 356 AlwNI CAG_nnn'CTG Cuts at: 0 976 1182 1521 1848 2110 2289 Size: 976 206 339 327 262 179 Fragments arranged by size: 976 339 327 262 206 179 ApoI r'AATT_y Cuts at: 0 196 1549 1622 2201 2289 Size: 196 1353 73 579 88 Fragments arranged by size: 1353 579 196 88 73 AseI AT'TA_AT Cuts at: 0 832 2289 Size: 832 1457 AvaI C'yCGr_G Cuts at: 0 574 1637 2289 Size: 574 1063 652 Fragments arranged by size: 1063 652 574 BanII G_rGCy'C Cuts at: 0 140 461 2289 Size: 140 321 1828 Fragments arranged by size: 1828 321 140 BbsI GAAGACnn'nnnn_ Cuts at: 0 35 287 2289 Size: 35 252 2002 Fragments arranged by size: 2002 252 35 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 524 558 2289 Size: 524 34 1731 Fragments arranged by size: 1731 524 34 BclI T'GATC_A Cuts at: 0 614 855 2289 Size: 614 241 1434 Fragments arranged by size: 1434 614 241 BlpI GC'TnA_GC Cuts at: 0 582 2289 Size: 582 1707 BmrI ACTGGGnnnn_n' Cuts at: 0 482 2289 Size: 482 1807 BmtI G_CTAG'C Cuts at: 0 1039 2289 Size: 1039 1250 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1529 2289 Size: 1529 760 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 778 887 1040 1522 1625 1870 2054 2289 Size: 778 109 153 482 103 245 184 235 Fragments arranged by size: 778 482 245 235 184 153 109 103 Bpu10I CC'TnA_GC Cuts at: 0 996 2289 Size: 996 1293 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2070 2289 Size: 2070 219 BsaI GGTCTCn'nnnn_ Cuts at: 0 1468 2289 Size: 1468 821 BsaBI GATnn'nnATC Cuts at: 0 1574 2289 Size: 1574 715 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2244 2274 2289 Size: 2244 30 15 Fragments arranged by size: 2244 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 139 886 1834 2167 2289 Size: 139 747 948 333 122 Fragments arranged by size: 948 747 333 139 122 BseYI C'CCAG_C Cuts at: 0 141 881 1031 1299 2050 2289 Size: 141 740 150 268 751 239 Fragments arranged by size: 751 740 268 239 150 141 BsiHKAI G_wGCw'C Cuts at: 0 461 1138 2084 2134 2289 Size: 461 677 946 50 155 Fragments arranged by size: 946 677 461 155 50 BsmI GAATG_Cn' Cuts at: 0 1118 1686 2289 Size: 1118 568 603 Fragments arranged by size: 1118 603 568 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1837 2289 Size: 1837 452 Bsp1286I G_dGCh'C Cuts at: 0 140 461 1138 2084 2134 2289 Size: 140 321 677 946 50 155 Fragments arranged by size: 946 677 321 155 140 50 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1038 1903 2078 2289 Size: 1038 865 175 211 Fragments arranged by size: 1038 865 211 175 BsrDI GCAATG_nn' Cuts at: 0 542 1049 1568 1659 2006 2289 Size: 542 507 519 91 347 283 Fragments arranged by size: 542 519 507 347 283 91 BstAPI GCAn_nnn'nTGC Cuts at: 0 976 1965 2289 Size: 976 989 324 Fragments arranged by size: 989 976 324 BstXI CCAn_nnnn'nTGG Cuts at: 0 2046 2289 Size: 2046 243 BstYI r'GATC_y Cuts at: 0 803 1718 1771 2000 2289 Size: 803 915 53 229 289 Fragments arranged by size: 915 803 289 229 53 Bsu36I CC'TnA_GG Cuts at: 0 23 2289 Size: 23 2266 BtgI C'CryG_G Cuts at: 0 92 965 2045 2289 Size: 92 873 1080 244 Fragments arranged by size: 1080 873 244 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2025 2289 Size: 2025 264 BtsI GCAGTG_nn' Cuts at: 0 2012 2289 Size: 2012 277 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1563 1598 2289 Size: 1563 35 691 Fragments arranged by size: 1563 691 35 EaeI y'GGCC_r Cuts at: 0 234 719 2289 Size: 234 485 1570 Fragments arranged by size: 1570 485 234 EarI CTCTTCn'nnn_ Cuts at: 0 116 449 2165 2219 2289 Size: 116 333 1716 54 70 Fragments arranged by size: 1716 333 116 70 54 EcoICRI GAG'CTC Cuts at: 0 459 2289 Size: 459 1830 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 778 887 894 1040 1304 1522 1625 1870 Size: 778 109 7 146 264 218 103 245 Cuts at: 1870 2054 2289 Size: 184 235 Fragments arranged by size: 778 264 245 235 218 184 146 109 103 7 EcoO109I rG'GnC_Cy Cuts at: 0 1279 2289 Size: 1279 1010 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 59 544 1108 2289 Size: 59 485 564 1181 Fragments arranged by size: 1181 564 485 59 FspI TGC'GCA Cuts at: 0 1921 1948 2289 Size: 1921 27 341 Fragments arranged by size: 1921 341 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 355 387 881 913 1204 1236 2289 Size: 355 32 494 32 291 32 1053 Fragments arranged by size: 1053 494 355 291 32 32 32 HincII GTy'rAC Cuts at: 0 429 2289 Size: 429 1860 HindIII A'AGCT_T Cuts at: 0 526 2289 Size: 526 1763 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1032 1434 1775 2289 Size: 1032 402 341 514 Fragments arranged by size: 1032 514 402 341 MscI TGG'CCA Cuts at: 0 236 721 2289 Size: 236 485 1568 Fragments arranged by size: 1568 485 236 MslI CAynn'nnrTG Cuts at: 0 610 2044 2289 Size: 610 1434 245 Fragments arranged by size: 1434 610 245 MspA1I CmG'CkG Cuts at: 0 624 645 822 877 951 973 2289 Size: 624 21 177 55 74 22 1316 Fragments arranged by size: 1316 624 177 74 55 22 21 NcoI C'CATG_G Cuts at: 0 92 965 2045 2289 Size: 92 873 1080 244 Fragments arranged by size: 1080 873 244 92 NdeI CA'TA_TG Cuts at: 0 1247 2289 Size: 1247 1042 NheI G'CTAG_C Cuts at: 0 1035 2289 Size: 1035 1254 NspI r_CATG'y Cuts at: 0 166 1743 2289 Size: 166 1577 546 Fragments arranged by size: 1577 546 166 PasI CC'CwG_GG Cuts at: 0 384 2289 Size: 384 1905 PflMI CCAn_nnn'nTGG Cuts at: 0 2110 2289 Size: 2110 179 PfoI T'CCnGG_A Cuts at: 0 1056 2289 Size: 1056 1233 PstI C_TGCA'G Cuts at: 0 876 1016 2289 Size: 876 140 1273 Fragments arranged by size: 1273 876 140 PvuII CAG'CTG Cuts at: 0 624 645 822 877 951 973 2289 Size: 624 21 177 55 74 22 1316 Fragments arranged by size: 1316 624 177 74 55 22 21 SacI G_AGCT'C Cuts at: 0 461 2289 Size: 461 1828 SapI GCTCTTCn'nnn_ Cuts at: 0 449 2289 Size: 449 1840 ScaI AGT'ACT Cuts at: 0 1975 2289 Size: 1975 314 SfcI C'TryA_G Cuts at: 0 766 872 1012 2289 Size: 766 106 140 1277 Fragments arranged by size: 1277 766 140 106 SmlI C'TyrA_G Cuts at: 0 2085 2289 Size: 2085 204 StyI C'CwwG_G Cuts at: 0 92 965 1083 1092 1370 2045 2289 Size: 92 873 118 9 278 675 244 Fragments arranged by size: 873 675 278 244 118 92 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1167 1239 1248 2289 Size: 1167 72 9 1041 Fragments arranged by size: 1167 1041 72 9 TatI w'GTAC_w Cuts at: 0 376 1871 1973 2289 Size: 376 1495 102 316 Fragments arranged by size: 1495 376 316 102 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2244 2276 2289 Size: 2244 32 13 Fragments arranged by size: 2244 32 13 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1090 2289 Size: 1090 1199 XmnI GAAnn'nnTTC Cuts at: 0 1684 2289 Size: 1684 605 Enzymes that do cut: AarI AcuI AlfI AlwNI ApoI AseI AvaI BanII BbsI BcgI BclI BlpI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I FalI FspI Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NdeI NheI NspI PasI PflMI PfoI PstI PvuII SacI SapI ScaI SfcI SmlI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BfrBI BglI BglII Bme1580I BmgBI BsaAI BsaHI BsaWI BsgI BsiEI BsiWI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI