Restriction map for
pFN21AB7278
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AflII AflIII
AhdI AocI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BseAI BsgI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MroI MseI MunI
NcoI NheI NruI NsiI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SalI ScaI SfiI SfuI SnaBI
SphI SspBI SspI StuI SwaI
TfiI Tru9I TspRI Tth111I XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AclI 485 AACGTT AA^CGTT
AcsI 943 AAATTC [GA]^AATT[CT]
AgeI 1287 ACCGGT A^CCGGT
Alw44I 1047 GTGCAC G^TGCAC
Aor51HI 550 AGCGCT AGC^GCT
ApaLI 1047 GTGCAC G^TGCAC
ApoI 943 AAATTC [GA]^AATT[CT]
BalI 791 TGGCCA TGG^CCA
BciVI 6 GGATAC GTATCC(6/5)
BsaI 253 GGTCTC GGTCTC(1/5)
BsaWI 1287 ACCGGT [AT]^CCGG[AT]
BsmBI 1129 GAGACG CGTCTC(1/5)
BspMI 94 ACCTGC ACCTGC(4/8)
BssHII 1562 GCGCGC G^CGCGC
BstAPI 821 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III 550 AGCGCT AGC^GCT
EcoNI 1191 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeII 486 ACGT A^CGT
MluNI 791 TGGCCA TGG^CCA
MscI 791 TGGCCA TGG^CCA
MslI 1380 CACTTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1927 CATATG CA^TATG
NspI 466 ACATGC [GA]CATG^[CT]
PinAI 1287 ACCGGT A^CCGGT
Psp1406I 485 AACGTT AA^CGTT
SacI 1752 GAGCTC GAGCT^C
SapI 295 GAAGAGC GCTCTTC(1/4)
SbfI 1191 CCTGCAGG CCTGCA^GG
SexAI 1916 ACCTGGT A^CC[AT]GGT
SpeI 378 ACTAGT A^CTAGT
Sse8387I 1191 CCTGCAGG CCTGCA^GG
Tsp45I 1284 GTCAC ^GT[GC]AC
Tsp509I 944 AATT ^AATT
XbaI 427 TCTAGA T^CTAGA
XcmI 1031 CCAAGGAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 183 GTCTAC GT^[AC][GT]AC
220 GTAGAC
AlwI 431 GATCC GGATC(4/5)
1038 GGATC
AlwNI 822 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1552 CAGAAGCTG
AspHI 1047 GTGCAC G[AT]GC[AT]^C
1752 GAGCTC
BbvCI 227 CCTCAGC CCTCAGC(-5/-2)
846 CCTCAGC
BcgI 1076 GCAGCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1148 GCAGCGCCTTCG
BglI 1177 GCCCGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1335 GCCCAGCCGGC
BpmI 1645 CTCCAG CTGGAG(16/14)
2043 CTCCAG
BsiHKAI 1047 GTGCAC G[AT]GC[AT]^C
1752 GAGCTC
BsmI 111 GAATGC GAATGC(1/-1)
475 GAATGC
BsrDI 1889 GCAATG GCAATG(2/0)
2012 GCAATG
BstXI 794 CCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1239 CCAGGACGCTGG
BstYI 430 AGATCC [GA]^GATC[CT]
1038 GGATCT
DpnII 431 GATC ^GATC
1039 GATC
MboI 431 GATC ^GATC
1039 GATC
MflI 430 AGATCC [GA]^GATC[CT]
1038 GGATCT
NdeII 431 GATC ^GATC
1039 GATC
NotI 1276 GCGGCCGC GC^GGCCGC
1786 GCGGCCGC
PaeR7I 1345 CTCGAG C^TCGAG
1567 CTCGAG
PflMI 783 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1551 CCAGAAGCTGG
PpuMI 669 GGGTCCC [GA]G^G[AT]CC[CT]
1678 GGGTCCC
Sau3AI 431 GATC ^GATC
1039 GATC
SmaI 666 CCCGGG CCC^GGG
812 CCCGGG
TaqI 1346 TCGA T^CGA
1568 TCGA
TthHB8I 1346 TCGA T^CGA
1568 TCGA
Van91I 783 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1551 CCAGAAGCTGG
XhoI 1345 CTCGAG C^TCGAG
1567 CTCGAG
XhoII 430 AGATCC [GA]^GATC[CT]
1038 GGATCT
XmaCI 666 CCCGGG C^CCGGG
812 CCCGGG
XmaI 666 CCCGGG C^CCGGG
812 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AvaI AvaII BanI BanII
BbeI BbvI BcnI BfaI BmyI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NgoMIV NlaIII NlaIV PleI
PstI PvuII RsaI SacII Sau96I
ScrFI SfaNI SfcI SfoI SgrAI
SmlI StyI TseI